Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles.
Identifieur interne : 000F14 ( Ncbi/Checkpoint ); précédent : 000F13; suivant : 000F15Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles.
Auteurs : Ping Zhao [République populaire de Chine] ; Zhao-Ling Qin ; Jin-Shan Ke ; Yang Lu ; Min Liu ; Wei Pan ; Lan-Juan Zhao ; Jie Cao ; Zhong-Tian QiSource :
- FEBS letters [ 0014-5793 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cricetinae, Expression des gènes (génétique), Humains, Lignée cellulaire, Muscles (métabolisme), Petit ARN interférent (génétique), Petit ARN interférent (métabolisme), Petit ARN nucléaire (génétique), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Régions promotrices (génétique) (génétique), Souris, Vecteurs génétiques (génétique), Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
- génétique : Expression des gènes, Petit ARN interférent, Petit ARN nucléaire, Protéines nucléocapside, Protéines recombinantes, Protéines à fluorescence verte, Régions promotrices (génétique), Vecteurs génétiques, Virus du SRAS.
- métabolisme : Muscles, Petit ARN interférent, Protéines nucléocapside, Protéines recombinantes, Protéines à fluorescence verte, Virus du SRAS.
- Animaux, Cricetinae, Humains, Lignée cellulaire, Souris.
English descriptors
- KwdEn :
- Animals, Cell Line, Cricetinae, Gene Expression (genetics), Genetic Vectors (genetics), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Humans, Mice, Muscles (metabolism), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), Promoter Regions, Genetic (genetics), RNA, Small Interfering (genetics), RNA, Small Interfering (metabolism), RNA, Small Nuclear (genetics), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), SARS Virus (genetics), SARS Virus (metabolism).
- MESH :
- chemical , genetics : Green Fluorescent Proteins, Nucleocapsid Proteins, RNA, Small Interfering, RNA, Small Nuclear, Recombinant Proteins.
- genetics : Gene Expression, Genetic Vectors, Promoter Regions, Genetic, SARS Virus.
- chemical , metabolism : Green Fluorescent Proteins, Muscles, Nucleocapsid Proteins, RNA, Small Interfering, Recombinant Proteins, SARS Virus.
- Animals, Cell Line, Cricetinae, Humans, Mice.
Abstract
SARS-CoV is a newly identified coronavirus that causes severe acute respiratory syndrome (SARS). Currently, there is no effective method available for prophylaxis and treatment of SARS-CoV infections. In the present study, the influence of small interfering RNA (siRNA) on SARS-CoV nucleocapsid (N) protein expression was detected in cultured cells and mouse muscles. Four siRNA expression cassettes driven by mouse U6 promoter targeting SARS-CoV N gene were prepared, and their inhibitory effects on expression of N and enhanced green fluorescence protein (EGFP) fusion protein were observed. A candidate siRNA was proved to down-regulate N and EGFP expression actively in a sequence-specific manner. The expression vector of this siRNA was constructed and confirmed to reduce N and EGFP expression efficiently in both cultured cells and adult mouse muscles. Our findings suggest that the siRNA should provide the basis for prophylaxis and therapy of SARS-CoV infection in human.
DOI: 10.1016/j.febslet.2005.02.080
PubMed: 15848179
Affiliations:
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pubmed:15848179Le document en format XML
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of Microbiology, Second Military Medical University, Shanghai</wicri:regionArea><wicri:noRegion>Shanghai</wicri:noRegion></affiliation></author><author><name sortKey="Qin, Zhao Ling" sort="Qin, Zhao Ling" uniqKey="Qin Z" first="Zhao-Ling" last="Qin">Zhao-Ling Qin</name></author><author><name sortKey="Ke, Jin Shan" sort="Ke, Jin Shan" uniqKey="Ke J" first="Jin-Shan" last="Ke">Jin-Shan Ke</name></author><author><name sortKey="Lu, Yang" sort="Lu, Yang" uniqKey="Lu Y" first="Yang" last="Lu">Yang Lu</name></author><author><name sortKey="Liu, Min" sort="Liu, Min" uniqKey="Liu M" first="Min" last="Liu">Min Liu</name></author><author><name sortKey="Pan, Wei" sort="Pan, Wei" uniqKey="Pan W" first="Wei" last="Pan">Wei Pan</name></author><author><name sortKey="Zhao, Lan Juan" sort="Zhao, Lan Juan" uniqKey="Zhao L" first="Lan-Juan" last="Zhao">Lan-Juan Zhao</name></author><author><name sortKey="Cao, Jie" sort="Cao, Jie" uniqKey="Cao J" first="Jie" last="Cao">Jie Cao</name></author><author><name sortKey="Qi, Zhong Tian" sort="Qi, Zhong Tian" uniqKey="Qi Z" first="Zhong-Tian" last="Qi">Zhong-Tian Qi</name></author></analytic><series><title level="j">FEBS letters</title><idno type="ISSN">0014-5793</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Cricetinae</term><term>Gene Expression (genetics)</term><term>Genetic Vectors (genetics)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Humans</term><term>Mice</term><term>Muscles (metabolism)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (metabolism)</term><term>Promoter Regions, Genetic (genetics)</term><term>RNA, Small Interfering (genetics)</term><term>RNA, Small Interfering (metabolism)</term><term>RNA, Small Nuclear (genetics)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cricetinae</term><term>Expression des gènes (génétique)</term><term>Humains</term><term>Lignée cellulaire</term><term>Muscles (métabolisme)</term><term>Petit ARN interférent (génétique)</term><term>Petit ARN interférent (métabolisme)</term><term>Petit ARN nucléaire (génétique)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (métabolisme)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Protéines à fluorescence verte (génétique)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Régions promotrices (génétique) (génétique)</term><term>Souris</term><term>Vecteurs génétiques (génétique)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Nucleocapsid Proteins</term><term>RNA, Small Interfering</term><term>RNA, Small Nuclear</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression</term><term>Genetic Vectors</term><term>Promoter Regions, Genetic</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Expression des gènes</term><term>Petit ARN interférent</term><term>Petit ARN nucléaire</term><term>Protéines nucléocapside</term><term>Protéines recombinantes</term><term>Protéines à fluorescence verte</term><term>Régions promotrices (génétique)</term><term>Vecteurs génétiques</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Muscles</term><term>Nucleocapsid Proteins</term><term>RNA, Small Interfering</term><term>Recombinant Proteins</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Muscles</term><term>Petit ARN interférent</term><term>Protéines nucléocapside</term><term>Protéines recombinantes</term><term>Protéines à fluorescence verte</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Cricetinae</term><term>Humans</term><term>Mice</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cricetinae</term><term>Humains</term><term>Lignée cellulaire</term><term>Souris</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">SARS-CoV is a newly identified coronavirus that causes severe acute respiratory syndrome (SARS). Currently, there is no effective method available for prophylaxis and treatment of SARS-CoV infections. In the present study, the influence of small interfering RNA (siRNA) on SARS-CoV nucleocapsid (N) protein expression was detected in cultured cells and mouse muscles. Four siRNA expression cassettes driven by mouse U6 promoter targeting SARS-CoV N gene were prepared, and their inhibitory effects on expression of N and enhanced green fluorescence protein (EGFP) fusion protein were observed. A candidate siRNA was proved to down-regulate N and EGFP expression actively in a sequence-specific manner. The expression vector of this siRNA was constructed and confirmed to reduce N and EGFP expression efficiently in both cultured cells and adult mouse muscles. Our findings suggest that the siRNA should provide the basis for prophylaxis and therapy of SARS-CoV infection in human.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Cao, Jie" sort="Cao, Jie" uniqKey="Cao J" first="Jie" last="Cao">Jie Cao</name><name sortKey="Ke, Jin Shan" sort="Ke, Jin Shan" uniqKey="Ke J" first="Jin-Shan" last="Ke">Jin-Shan Ke</name><name sortKey="Liu, Min" sort="Liu, Min" uniqKey="Liu M" first="Min" last="Liu">Min Liu</name><name sortKey="Lu, Yang" sort="Lu, Yang" uniqKey="Lu Y" first="Yang" last="Lu">Yang Lu</name><name sortKey="Pan, Wei" sort="Pan, Wei" uniqKey="Pan W" first="Wei" last="Pan">Wei Pan</name><name sortKey="Qi, Zhong Tian" sort="Qi, Zhong Tian" uniqKey="Qi Z" first="Zhong-Tian" last="Qi">Zhong-Tian Qi</name><name sortKey="Qin, Zhao Ling" sort="Qin, Zhao Ling" uniqKey="Qin Z" first="Zhao-Ling" last="Qin">Zhao-Ling Qin</name><name sortKey="Zhao, Lan Juan" sort="Zhao, Lan Juan" uniqKey="Zhao L" first="Lan-Juan" last="Zhao">Lan-Juan Zhao</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zhao, Ping" sort="Zhao, Ping" uniqKey="Zhao P" first="Ping" last="Zhao">Ping Zhao</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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