Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles.
Identifieur interne : 002772 ( PubMed/Corpus ); précédent : 002771; suivant : 002773Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles.
Auteurs : Ping Zhao ; Zhao-Ling Qin ; Jin-Shan Ke ; Yang Lu ; Min Liu ; Wei Pan ; Lan-Juan Zhao ; Jie Cao ; Zhong-Tian QiSource :
- FEBS letters [ 0014-5793 ] ; 2005.
English descriptors
- KwdEn :
- Animals, Cell Line, Cricetinae, Gene Expression (genetics), Genetic Vectors (genetics), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), Humans, Mice, Muscles (metabolism), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), Promoter Regions, Genetic (genetics), RNA, Small Interfering (genetics), RNA, Small Interfering (metabolism), RNA, Small Nuclear (genetics), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), SARS Virus (genetics), SARS Virus (metabolism).
- MESH :
- chemical , genetics : Green Fluorescent Proteins, Nucleocapsid Proteins, RNA, Small Interfering, RNA, Small Nuclear, Recombinant Proteins.
- genetics : Gene Expression, Genetic Vectors, Promoter Regions, Genetic, SARS Virus.
- chemical , metabolism : Green Fluorescent Proteins, Muscles, Nucleocapsid Proteins, RNA, Small Interfering, Recombinant Proteins, SARS Virus.
- Animals, Cell Line, Cricetinae, Humans, Mice.
Abstract
SARS-CoV is a newly identified coronavirus that causes severe acute respiratory syndrome (SARS). Currently, there is no effective method available for prophylaxis and treatment of SARS-CoV infections. In the present study, the influence of small interfering RNA (siRNA) on SARS-CoV nucleocapsid (N) protein expression was detected in cultured cells and mouse muscles. Four siRNA expression cassettes driven by mouse U6 promoter targeting SARS-CoV N gene were prepared, and their inhibitory effects on expression of N and enhanced green fluorescence protein (EGFP) fusion protein were observed. A candidate siRNA was proved to down-regulate N and EGFP expression actively in a sequence-specific manner. The expression vector of this siRNA was constructed and confirmed to reduce N and EGFP expression efficiently in both cultured cells and adult mouse muscles. Our findings suggest that the siRNA should provide the basis for prophylaxis and therapy of SARS-CoV infection in human.
DOI: 10.1016/j.febslet.2005.02.080
PubMed: 15848179
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles.</title><author><name sortKey="Zhao, Ping" sort="Zhao, Ping" uniqKey="Zhao P" first="Ping" last="Zhao">Ping Zhao</name><affiliation><nlm:affiliation>Department of Microbiology, Second Military Medical University, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Qin, Zhao Ling" sort="Qin, Zhao Ling" uniqKey="Qin Z" first="Zhao-Ling" last="Qin">Zhao-Ling Qin</name></author><author><name sortKey="Ke, Jin Shan" sort="Ke, Jin Shan" uniqKey="Ke J" first="Jin-Shan" last="Ke">Jin-Shan Ke</name></author><author><name sortKey="Lu, Yang" sort="Lu, Yang" uniqKey="Lu Y" first="Yang" last="Lu">Yang Lu</name></author><author><name sortKey="Liu, Min" sort="Liu, Min" uniqKey="Liu M" first="Min" last="Liu">Min Liu</name></author><author><name sortKey="Pan, Wei" sort="Pan, Wei" uniqKey="Pan W" first="Wei" last="Pan">Wei Pan</name></author><author><name sortKey="Zhao, Lan Juan" sort="Zhao, Lan Juan" uniqKey="Zhao L" first="Lan-Juan" last="Zhao">Lan-Juan Zhao</name></author><author><name sortKey="Cao, Jie" sort="Cao, Jie" uniqKey="Cao J" first="Jie" last="Cao">Jie Cao</name></author><author><name sortKey="Qi, Zhong Tian" sort="Qi, Zhong Tian" uniqKey="Qi Z" first="Zhong-Tian" last="Qi">Zhong-Tian Qi</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15848179</idno><idno type="pmid">15848179</idno><idno type="doi">10.1016/j.febslet.2005.02.080</idno><idno type="wicri:Area/PubMed/Corpus">002772</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002772</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles.</title><author><name sortKey="Zhao, Ping" sort="Zhao, Ping" uniqKey="Zhao P" first="Ping" last="Zhao">Ping Zhao</name><affiliation><nlm:affiliation>Department of Microbiology, Second Military Medical University, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Qin, Zhao Ling" sort="Qin, Zhao Ling" uniqKey="Qin Z" first="Zhao-Ling" last="Qin">Zhao-Ling Qin</name></author><author><name sortKey="Ke, Jin Shan" sort="Ke, Jin Shan" uniqKey="Ke J" first="Jin-Shan" last="Ke">Jin-Shan Ke</name></author><author><name sortKey="Lu, Yang" sort="Lu, Yang" uniqKey="Lu Y" first="Yang" last="Lu">Yang Lu</name></author><author><name sortKey="Liu, Min" sort="Liu, Min" uniqKey="Liu M" first="Min" last="Liu">Min Liu</name></author><author><name sortKey="Pan, Wei" sort="Pan, Wei" uniqKey="Pan W" first="Wei" last="Pan">Wei Pan</name></author><author><name sortKey="Zhao, Lan Juan" sort="Zhao, Lan Juan" uniqKey="Zhao L" first="Lan-Juan" last="Zhao">Lan-Juan Zhao</name></author><author><name sortKey="Cao, Jie" sort="Cao, Jie" uniqKey="Cao J" first="Jie" last="Cao">Jie Cao</name></author><author><name sortKey="Qi, Zhong Tian" sort="Qi, Zhong Tian" uniqKey="Qi Z" first="Zhong-Tian" last="Qi">Zhong-Tian Qi</name></author></analytic><series><title level="j">FEBS letters</title><idno type="ISSN">0014-5793</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Cricetinae</term><term>Gene Expression (genetics)</term><term>Genetic Vectors (genetics)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>Humans</term><term>Mice</term><term>Muscles (metabolism)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (metabolism)</term><term>Promoter Regions, Genetic (genetics)</term><term>RNA, Small Interfering (genetics)</term><term>RNA, Small Interfering (metabolism)</term><term>RNA, Small Nuclear (genetics)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Nucleocapsid Proteins</term><term>RNA, Small Interfering</term><term>RNA, Small Nuclear</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression</term><term>Genetic Vectors</term><term>Promoter Regions, Genetic</term><term>SARS Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Muscles</term><term>Nucleocapsid Proteins</term><term>RNA, Small Interfering</term><term>Recombinant Proteins</term><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Cricetinae</term><term>Humans</term><term>Mice</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">SARS-CoV is a newly identified coronavirus that causes severe acute respiratory syndrome (SARS). Currently, there is no effective method available for prophylaxis and treatment of SARS-CoV infections. In the present study, the influence of small interfering RNA (siRNA) on SARS-CoV nucleocapsid (N) protein expression was detected in cultured cells and mouse muscles. Four siRNA expression cassettes driven by mouse U6 promoter targeting SARS-CoV N gene were prepared, and their inhibitory effects on expression of N and enhanced green fluorescence protein (EGFP) fusion protein were observed. A candidate siRNA was proved to down-regulate N and EGFP expression actively in a sequence-specific manner. The expression vector of this siRNA was constructed and confirmed to reduce N and EGFP expression efficiently in both cultured cells and adult mouse muscles. Our findings suggest that the siRNA should provide the basis for prophylaxis and therapy of SARS-CoV infection in human.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15848179</PMID><DateCompleted><Year>2005</Year><Month>05</Month><Day>31</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>18</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0014-5793</ISSN><JournalIssue CitedMedium="Print"><Volume>579</Volume><Issue>11</Issue><PubDate><Year>2005</Year><Month>Apr</Month><Day>25</Day></PubDate></JournalIssue><Title>FEBS letters</Title><ISOAbbreviation>FEBS Lett.</ISOAbbreviation></Journal><ArticleTitle>Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles.</ArticleTitle><Pagination><MedlinePgn>2404-10</MedlinePgn></Pagination><Abstract><AbstractText>SARS-CoV is a newly identified coronavirus that causes severe acute respiratory syndrome (SARS). Currently, there is no effective method available for prophylaxis and treatment of SARS-CoV infections. In the present study, the influence of small interfering RNA (siRNA) on SARS-CoV nucleocapsid (N) protein expression was detected in cultured cells and mouse muscles. Four siRNA expression cassettes driven by mouse U6 promoter targeting SARS-CoV N gene were prepared, and their inhibitory effects on expression of N and enhanced green fluorescence protein (EGFP) fusion protein were observed. A candidate siRNA was proved to down-regulate N and EGFP expression actively in a sequence-specific manner. The expression vector of this siRNA was constructed and confirmed to reduce N and EGFP expression efficiently in both cultured cells and adult mouse muscles. Our findings suggest that the siRNA should provide the basis for prophylaxis and therapy of SARS-CoV infection in human.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Ping</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Second Military Medical University, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qin</LastName><ForeName>Zhao-Ling</ForeName><Initials>ZL</Initials></Author><Author ValidYN="Y"><LastName>Ke</LastName><ForeName>Jin-Shan</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Yang</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Min</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Pan</LastName><ForeName>Wei</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Lan-Juan</ForeName><Initials>LJ</Initials></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Jie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Qi</LastName><ForeName>Zhong-Tian</ForeName><Initials>ZT</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>FEBS Lett</MedlineTA><NlmUniqueID>0155157</NlmUniqueID><ISSNLinking>0014-5793</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D034741">RNA, Small 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MajorTopicYN="N">Cricetinae</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049452" MajorTopicYN="N">Green Fluorescent Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009132" MajorTopicYN="N">Muscles</DescriptorName><QualifierName UI="Q000378" 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