Retroviruses pseudotyped with the severe acute respiratory syndrome coronavirus spike protein efficiently infect cells expressing angiotensin-converting enzyme 2.
Identifieur interne : 000A74 ( Ncbi/Checkpoint ); précédent : 000A73; suivant : 000A75Retroviruses pseudotyped with the severe acute respiratory syndrome coronavirus spike protein efficiently infect cells expressing angiotensin-converting enzyme 2.
Auteurs : Michael J. Moore [États-Unis] ; Tatyana Dorfman ; Wenhui Li ; Swee Kee Wong ; Yanhan Li ; Jens H. Kuhn ; James Coderre ; Natalya Vasilieva ; Zhongchao Han ; Thomas C. Greenough ; Michael Farzan ; Hyeryun ChoeSource :
- Journal of virology [ 0022-538X ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Carboxypeptidases (génétique), Carboxypeptidases (métabolisme), Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Humains, Lignée cellulaire, Peptidyl-Dipeptidase A, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Récepteurs viraux (métabolisme), Réplication virale, Séquence d'acides aminés, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique), Virion (), Virion (métabolisme), Virus de l'immunodéficience simienne (génétique), Virus de l'immunodéficience simienne (métabolisme), Virus de l'immunodéficience simienne (physiologie), Virus de la leucémie murine (génétique), Virus de la leucémie murine (métabolisme), Virus de la leucémie murine (physiologie), Virus du SRAS (génétique).
- MESH :
- génétique : Carboxypeptidases, Glycoprotéines membranaires, Protéines de l'enveloppe virale, VIH-1 (Virus de l'Immunodéficience Humaine de type 1), Virus de l'immunodéficience simienne, Virus de la leucémie murine, Virus du SRAS.
- métabolisme : Carboxypeptidases, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Récepteurs viraux, Virion, Virus de l'immunodéficience simienne, Virus de la leucémie murine.
- physiologie : Virus de l'immunodéficience simienne, Virus de la leucémie murine.
- Animaux, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Peptidyl-Dipeptidase A, Réplication virale, Séquence d'acides aminés, Virion.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Carboxypeptidases (genetics), Carboxypeptidases (metabolism), Cell Line, HIV-1 (genetics), Humans, Leukemia Virus, Murine (genetics), Leukemia Virus, Murine (metabolism), Leukemia Virus, Murine (physiology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Molecular Sequence Data, Peptidyl-Dipeptidase A, Receptors, Virus (metabolism), SARS Virus (genetics), Simian Immunodeficiency Virus (genetics), Simian Immunodeficiency Virus (metabolism), Simian Immunodeficiency Virus (physiology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Virion (chemistry), Virion (metabolism), Virus Replication.
- MESH :
- chemical , genetics : Carboxypeptidases, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : Carboxypeptidases, Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins.
- chemistry : Virion.
- genetics : HIV-1, Leukemia Virus, Murine, SARS Virus, Simian Immunodeficiency Virus.
- metabolism : Leukemia Virus, Murine, Simian Immunodeficiency Virus, Virion.
- physiology : Leukemia Virus, Murine, Simian Immunodeficiency Virus.
- Amino Acid Sequence, Animals, Cell Line, Humans, Molecular Sequence Data, Peptidyl-Dipeptidase A, Spike Glycoprotein, Coronavirus, Virus Replication.
Abstract
Infection of receptor-bearing cells by coronaviruses is mediated by their spike (S) proteins. The coronavirus (SARS-CoV) that causes severe acute respiratory syndrome (SARS) infects cells expressing the receptor angiotensin-converting enzyme 2 (ACE2). Here we show that codon optimization of the SARS-CoV S-protein gene substantially enhanced S-protein expression. We also found that two retroviruses, simian immunodeficiency virus (SIV) and murine leukemia virus, both expressing green fluorescent protein and pseudotyped with SARS-CoV S protein or S-protein variants, efficiently infected HEK293T cells stably expressing ACE2. Infection mediated by an S-protein variant whose cytoplasmic domain had been truncated and altered to include a fragment of the cytoplasmic tail of the human immunodeficiency virus type 1 envelope glycoprotein was, in both cases, substantially more efficient than that mediated by wild-type S protein. Using S-protein-pseudotyped SIV, we found that the enzymatic activity of ACE2 made no contribution to S-protein-mediated infection. Finally, we show that a soluble and catalytically inactive form of ACE2 potently blocked infection by S-protein-pseudotyped retrovirus and by SARS-CoV. These results permit studies of SARS-CoV entry inhibitors without the use of live virus and suggest a candidate therapy for SARS.
DOI: 10.1128/JVI.78.19.10628-10635.2004
PubMed: 15367630
Affiliations:
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Moore</name><affiliation wicri:level="2"><nlm:affiliation>Partners AIDS Research Center, Brigham and Women's Hospital, and Department of Medicine, Harvard Medical School, Boston, MA, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Partners AIDS Research Center, Brigham and Women's Hospital, and Department of Medicine, Harvard Medical School, Boston, MA</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Dorfman, Tatyana" sort="Dorfman, Tatyana" uniqKey="Dorfman T" first="Tatyana" last="Dorfman">Tatyana Dorfman</name></author><author><name sortKey="Li, Wenhui" sort="Li, Wenhui" uniqKey="Li W" first="Wenhui" last="Li">Wenhui Li</name></author><author><name sortKey="Wong, Swee Kee" sort="Wong, Swee Kee" uniqKey="Wong S" first="Swee Kee" last="Wong">Swee Kee Wong</name></author><author><name sortKey="Li, Yanhan" sort="Li, Yanhan" uniqKey="Li Y" first="Yanhan" last="Li">Yanhan Li</name></author><author><name sortKey="Kuhn, Jens H" sort="Kuhn, Jens H" uniqKey="Kuhn J" first="Jens H" last="Kuhn">Jens H. Kuhn</name></author><author><name sortKey="Coderre, James" sort="Coderre, James" uniqKey="Coderre J" first="James" last="Coderre">James Coderre</name></author><author><name sortKey="Vasilieva, Natalya" sort="Vasilieva, Natalya" uniqKey="Vasilieva N" first="Natalya" last="Vasilieva">Natalya Vasilieva</name></author><author><name sortKey="Han, Zhongchao" sort="Han, Zhongchao" uniqKey="Han Z" first="Zhongchao" last="Han">Zhongchao Han</name></author><author><name sortKey="Greenough, Thomas C" sort="Greenough, Thomas C" uniqKey="Greenough T" first="Thomas C" last="Greenough">Thomas C. Greenough</name></author><author><name sortKey="Farzan, Michael" sort="Farzan, Michael" uniqKey="Farzan M" first="Michael" last="Farzan">Michael Farzan</name></author><author><name sortKey="Choe, Hyeryun" sort="Choe, Hyeryun" uniqKey="Choe H" first="Hyeryun" last="Choe">Hyeryun Choe</name></author></analytic><series><title level="j">Journal of virology</title><idno type="ISSN">0022-538X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Carboxypeptidases (genetics)</term><term>Carboxypeptidases (metabolism)</term><term>Cell Line</term><term>HIV-1 (genetics)</term><term>Humans</term><term>Leukemia Virus, Murine (genetics)</term><term>Leukemia Virus, Murine (metabolism)</term><term>Leukemia Virus, Murine (physiology)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (metabolism)</term><term>Molecular Sequence Data</term><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus (metabolism)</term><term>SARS Virus (genetics)</term><term>Simian Immunodeficiency Virus (genetics)</term><term>Simian Immunodeficiency Virus (metabolism)</term><term>Simian Immunodeficiency Virus (physiology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (metabolism)</term><term>Virion (chemistry)</term><term>Virion (metabolism)</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Carboxypeptidases (génétique)</term><term>Carboxypeptidases (métabolisme)</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Lignée cellulaire</term><term>Peptidyl-Dipeptidase A</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Récepteurs viraux (métabolisme)</term><term>Réplication virale</term><term>Séquence d'acides aminés</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term><term>Virion ()</term><term>Virion (métabolisme)</term><term>Virus de l'immunodéficience simienne (génétique)</term><term>Virus de l'immunodéficience simienne (métabolisme)</term><term>Virus de l'immunodéficience simienne (physiologie)</term><term>Virus de la leucémie murine (génétique)</term><term>Virus de la leucémie murine (métabolisme)</term><term>Virus de la leucémie murine (physiologie)</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carboxypeptidases</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carboxypeptidases</term><term>Membrane Glycoproteins</term><term>Receptors, Virus</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Virion</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV-1</term><term>Leukemia Virus, Murine</term><term>SARS Virus</term><term>Simian Immunodeficiency Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Carboxypeptidases</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term><term>Virus de l'immunodéficience simienne</term><term>Virus de la leucémie murine</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Leukemia Virus, Murine</term><term>Simian Immunodeficiency Virus</term><term>Virion</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Carboxypeptidases</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Récepteurs viraux</term><term>Virion</term><term>Virus de l'immunodéficience simienne</term><term>Virus de la leucémie murine</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus de l'immunodéficience simienne</term><term>Virus de la leucémie murine</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Leukemia Virus, Murine</term><term>Simian Immunodeficiency Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cell Line</term><term>Humans</term><term>Molecular Sequence Data</term><term>Peptidyl-Dipeptidase A</term><term>Spike Glycoprotein, Coronavirus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Lignée cellulaire</term><term>Peptidyl-Dipeptidase A</term><term>Réplication virale</term><term>Séquence d'acides aminés</term><term>Virion</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Infection of receptor-bearing cells by coronaviruses is mediated by their spike (S) proteins. The coronavirus (SARS-CoV) that causes severe acute respiratory syndrome (SARS) infects cells expressing the receptor angiotensin-converting enzyme 2 (ACE2). Here we show that codon optimization of the SARS-CoV S-protein gene substantially enhanced S-protein expression. We also found that two retroviruses, simian immunodeficiency virus (SIV) and murine leukemia virus, both expressing green fluorescent protein and pseudotyped with SARS-CoV S protein or S-protein variants, efficiently infected HEK293T cells stably expressing ACE2. Infection mediated by an S-protein variant whose cytoplasmic domain had been truncated and altered to include a fragment of the cytoplasmic tail of the human immunodeficiency virus type 1 envelope glycoprotein was, in both cases, substantially more efficient than that mediated by wild-type S protein. Using S-protein-pseudotyped SIV, we found that the enzymatic activity of ACE2 made no contribution to S-protein-mediated infection. Finally, we show that a soluble and catalytically inactive form of ACE2 potently blocked infection by S-protein-pseudotyped retrovirus and by SARS-CoV. These results permit studies of SARS-CoV entry inhibitors without the use of live virus and suggest a candidate therapy for SARS.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region></list><tree><noCountry><name sortKey="Choe, Hyeryun" sort="Choe, Hyeryun" uniqKey="Choe H" first="Hyeryun" last="Choe">Hyeryun Choe</name><name sortKey="Coderre, James" sort="Coderre, James" uniqKey="Coderre J" first="James" last="Coderre">James Coderre</name><name sortKey="Dorfman, Tatyana" sort="Dorfman, Tatyana" uniqKey="Dorfman T" first="Tatyana" last="Dorfman">Tatyana Dorfman</name><name sortKey="Farzan, Michael" sort="Farzan, Michael" uniqKey="Farzan M" first="Michael" last="Farzan">Michael Farzan</name><name sortKey="Greenough, Thomas C" sort="Greenough, Thomas C" uniqKey="Greenough T" first="Thomas C" last="Greenough">Thomas C. Greenough</name><name sortKey="Han, Zhongchao" sort="Han, Zhongchao" uniqKey="Han Z" first="Zhongchao" last="Han">Zhongchao Han</name><name sortKey="Kuhn, Jens H" sort="Kuhn, Jens H" uniqKey="Kuhn J" first="Jens H" last="Kuhn">Jens H. Kuhn</name><name sortKey="Li, Wenhui" sort="Li, Wenhui" uniqKey="Li W" first="Wenhui" last="Li">Wenhui Li</name><name sortKey="Li, Yanhan" sort="Li, Yanhan" uniqKey="Li Y" first="Yanhan" last="Li">Yanhan Li</name><name sortKey="Vasilieva, Natalya" sort="Vasilieva, Natalya" uniqKey="Vasilieva N" first="Natalya" last="Vasilieva">Natalya Vasilieva</name><name sortKey="Wong, Swee Kee" sort="Wong, Swee Kee" uniqKey="Wong S" first="Swee Kee" last="Wong">Swee Kee Wong</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Moore, Michael J" sort="Moore, Michael J" uniqKey="Moore M" first="Michael J" last="Moore">Michael J. Moore</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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