A single immunization with a rhabdovirus-based vector expressing severe acute respiratory syndrome coronavirus (SARS-CoV) S protein results in the production of high levels of SARs-CoV-neutralizing antibodies
Identifieur interne : 005237 ( Main/Merge ); précédent : 005236; suivant : 005238A single immunization with a rhabdovirus-based vector expressing severe acute respiratory syndrome coronavirus (SARS-CoV) S protein results in the production of high levels of SARs-CoV-neutralizing antibodies
Auteurs : Milosz Faber [États-Unis] ; Elaine W. Lamirande [États-Unis] ; Anjeanette Roberts [États-Unis] ; Amy B. Rice [États-Unis] ; Hilary Koprowski [États-Unis] ; Bernhard Dietzschold [États-Unis] ; Matthias J. Schnell [États-Unis]Source :
- Journal of general virology [ 0022-1317 ] ; 2005.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Vaccin.
English descriptors
- KwdEn :
Abstract
Foreign viral proteins expressed by rabies virus (RV) have been shown to induce potent humoral and cellular immune responses in immunized animals. In addition, highly attenuated and, therefore, very safe RV-based vectors have been constructed. Here, an RV-based vaccine vehicle was utilized as a novel vaccine against severe acute respiratory syndrome coronavirus (SARS-CoV). For this approach, the SARS-CoV nucleocapsid protein (N) or envelope spike protein (S) genes were cloned between the RV glycoprotein G and polymerase L genes. Recombinant vectors expressing SARS-CoV N or S protein were recovered and their immunogenicity was studied in mice. A single inoculation with the RV-based vaccine expressing SARS-CoV S protein induced a strong SARS-CoV-neutralizing antibody response. The ability of the RV-SARS-CoV S vector to confer immunity after a single inoculation makes this live vaccine a promising candidate for eradication of SARS-CoV in animal reservoirs, thereby reducing the risk of transmitting the infection to humans.
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Pascal:05-0229124Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Immunization</term>
<term>Microbiology</term>
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<term>Severe acute respiratory syndrome virus</term>
<term>Vaccine</term>
<term>Vector</term>
<term>Virology</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term>
<term>Immunisation</term>
<term>Vaccin</term>
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<term>Protéine</term>
<term>Anticorps neutralisant</term>
<term>Microbiologie</term>
<term>Virologie</term>
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<front><div type="abstract" xml:lang="en">Foreign viral proteins expressed by rabies virus (RV) have been shown to induce potent humoral and cellular immune responses in immunized animals. In addition, highly attenuated and, therefore, very safe RV-based vectors have been constructed. Here, an RV-based vaccine vehicle was utilized as a novel vaccine against severe acute respiratory syndrome coronavirus (SARS-CoV). For this approach, the SARS-CoV nucleocapsid protein (N) or envelope spike protein (S) genes were cloned between the RV glycoprotein G and polymerase L genes. Recombinant vectors expressing SARS-CoV N or S protein were recovered and their immunogenicity was studied in mice. A single inoculation with the RV-based vaccine expressing SARS-CoV S protein induced a strong SARS-CoV-neutralizing antibody response. The ability of the RV-SARS-CoV S vector to confer immunity after a single inoculation makes this live vaccine a promising candidate for eradication of SARS-CoV in animal reservoirs, thereby reducing the risk of transmitting the infection to humans.</div>
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<tree><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Faber, Milosz" sort="Faber, Milosz" uniqKey="Faber M" first="Milosz" last="Faber">Milosz Faber</name>
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<name sortKey="Schnell, Matthias J" sort="Schnell, Matthias J" uniqKey="Schnell M" first="Matthias J." last="Schnell">Matthias J. Schnell</name>
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