Detection of severe acute respiratory syndrome coronavirus RNA in plasma during the course of infection
Identifieur interne : 005195 ( Main/Merge ); précédent : 005194; suivant : 005196Detection of severe acute respiratory syndrome coronavirus RNA in plasma during the course of infection
Auteurs : Wei-Kung Wang [Taïwan] ; Chi-Tai Fang [Taïwan] ; Hui-Ling Chen [Taïwan] ; Chao-Fu Yang [Taïwan] ; Yee-Chun Chen [Taïwan] ; Mei-Ling Chen [Taïwan] ; Shey-Ying Chen [Taïwan] ; Jyh-Yuan Yang [Taïwan] ; Jih-Hui Lin [Taïwan] ; Pan-Chyr Yang [Taïwan] ; Shan-Chwen Chang [Taïwan]Source :
- Journal of clinical microbiology : (Print) [ 0095-1137 ] ; 2005.
Descripteurs français
- Pascal (Inist)
English descriptors
Abstract
We examined severe acute respiratory syndrome-associated coronavirus (SARS-CoV) RNA in plasma of 32 patients (probable SARS cases) by a quantitative real-time reverse transcription-PCR assay and reported that the highest detection rate, 75%, was found between day 5 and day 7 of illness, followed by rates of 64, 50, and 38% found between day 8 and day 11, day 2 and day 4, and day 12 and day 16, respectively. Analysis of sequential SARS-CoV load in plasma from six cases revealed different patterns of viremia, with the peak between day 4 and day 8. Our findings of the high detection rate of SARS-CoV RNA in plasma before day 11, together with the relative convenience of collecting and handling plasma, suggest that plasma can be used for early diagnosis of SARS.
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<front><div type="abstract" xml:lang="en">We examined severe acute respiratory syndrome-associated coronavirus (SARS-CoV) RNA in plasma of 32 patients (probable SARS cases) by a quantitative real-time reverse transcription-PCR assay and reported that the highest detection rate, 75%, was found between day 5 and day 7 of illness, followed by rates of 64, 50, and 38% found between day 8 and day 11, day 2 and day 4, and day 12 and day 16, respectively. Analysis of sequential SARS-CoV load in plasma from six cases revealed different patterns of viremia, with the peak between day 4 and day 8. Our findings of the high detection rate of SARS-CoV RNA in plasma before day 11, together with the relative convenience of collecting and handling plasma, suggest that plasma can be used for early diagnosis of SARS.</div>
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<name sortKey="Chen, Hui Ling" sort="Chen, Hui Ling" uniqKey="Chen H" first="Hui-Ling" last="Chen">Hui-Ling Chen</name>
<name sortKey="Chen, Mei Ling" sort="Chen, Mei Ling" uniqKey="Chen M" first="Mei-Ling" last="Chen">Mei-Ling Chen</name>
<name sortKey="Chen, Shey Ying" sort="Chen, Shey Ying" uniqKey="Chen S" first="Shey-Ying" last="Chen">Shey-Ying Chen</name>
<name sortKey="Chen, Yee Chun" sort="Chen, Yee Chun" uniqKey="Chen Y" first="Yee-Chun" last="Chen">Yee-Chun Chen</name>
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<name sortKey="Lin, Jih Hui" sort="Lin, Jih Hui" uniqKey="Lin J" first="Jih-Hui" last="Lin">Jih-Hui Lin</name>
<name sortKey="Wang, Wei Kung" sort="Wang, Wei Kung" uniqKey="Wang W" first="Wei-Kung" last="Wang">Wei-Kung Wang</name>
<name sortKey="Yang, Chao Fu" sort="Yang, Chao Fu" uniqKey="Yang C" first="Chao-Fu" last="Yang">Chao-Fu Yang</name>
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