Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR
Identifieur interne : 004E42 ( Main/Merge ); précédent : 004E41; suivant : 004E43Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR
Auteurs : Maria Cristina Keightley [États-Unis] ; Peter Sillekens [Pays-Bas] ; Wim Schippers [Pays-Bas] ; Charles Rinaldo [États-Unis] ; Kirsten St. George [États-Unis]Source :
- Journal of Medical Virology [ 0146-6615 ] ; 2005-11.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- diagnostic : Syndrome respiratoire aigu sévère.
- isolement et purification : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Humains, RT-PCR, Techniques d'amplification d'acides nucléiques.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- diagnosis : Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- methods : Nucleic Acid Amplification Techniques.
- virology : Severe Acute Respiratory Syndrome.
- Teeft :
- Assay, Chan, Clin, Clin microbiol, Clinical presentation, Coronavirus, Dean erdman, Different combinations, Dilution series, Disease control, Disease onset, Drosten, Early detection, Early diagnosis, Emery, European quality assurance, Genome, Greater sensitivity, Greatest sensitivity, High titer, Hong kong, Humans, Leader sequence, Lower case, Microbiol, Molecular beacons, Molecular tests, Nasba, Nasba application, Nasba assay, Nasba detection, Novel coronavirus, Nucleic, Nucleic acid, Peiris, Polymerase, Polymerase gene, Primer, Primer sets, Respiratory syndrome, Respiratory syndrome coronavirus, Respiratory syndrome diagnosis, Reverse Transcriptase Polymerase Chain Reaction, Sars, Sars coronavirus, Sars patients, Syndrome, Taqman, Taqman sars, Taqman test, Urbani, Urbani strain, Viral, Viral load, Whole genome urbani strain, World health organization multicentre.
Abstract
Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS‐CoV) RNA using real time reverse transcription polymerase chain reaction (RT‐PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real‐time nucleic acid sequence‐based amplification (NASBA) test for SARS‐CoV. A number of primer/beacon sets were designed to target different regions of the SARS‐CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT‐PCR assays. A multi‐target real‐time NASBA application was developed for detection of SARS‐CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real‐time NASBA assay demonstrates equivalent sensitivity when compared to testing by real‐time RT‐PCR. A multi‐target real‐time NASBA assay has been successfully developed for the sensitive detection of SARS‐CoV. J. Med. Virol. 77:602–608, 2005. © 2005 Wiley‐Liss, inc.
Url:
DOI: 10.1002/jmv.20498
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titer</term><term>Hong kong</term><term>Humans</term><term>Leader sequence</term><term>Lower case</term><term>Microbiol</term><term>Molecular beacons</term><term>Molecular tests</term><term>Nasba</term><term>Nasba application</term><term>Nasba assay</term><term>Nasba detection</term><term>Novel coronavirus</term><term>Nucleic</term><term>Nucleic acid</term><term>Peiris</term><term>Polymerase</term><term>Polymerase gene</term><term>Primer</term><term>Primer sets</term><term>Respiratory syndrome</term><term>Respiratory syndrome coronavirus</term><term>Respiratory syndrome diagnosis</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sars</term><term>Sars coronavirus</term><term>Sars patients</term><term>Syndrome</term><term>Taqman</term><term>Taqman sars</term><term>Taqman test</term><term>Urbani</term><term>Urbani strain</term><term>Viral</term><term>Viral load</term><term>Whole genome urbani strain</term><term>World health organization multicentre</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>RT-PCR</term><term>Techniques d'amplification d'acides nucléiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS‐CoV) RNA using real time reverse transcription polymerase chain reaction (RT‐PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real‐time nucleic acid sequence‐based amplification (NASBA) test for SARS‐CoV. A number of primer/beacon sets were designed to target different regions of the SARS‐CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT‐PCR assays. A multi‐target real‐time NASBA application was developed for detection of SARS‐CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real‐time NASBA assay demonstrates equivalent sensitivity when compared to testing by real‐time RT‐PCR. A multi‐target real‐time NASBA assay has been successfully developed for the sensitive detection of SARS‐CoV. J. Med. Virol. 77:602–608, 2005. © 2005 Wiley‐Liss, inc.</div></front></TEI><double doi="10.1002/jmv.20498"><ISTEX><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR</title><author><name sortKey="Keightley, Maria Cristina" sort="Keightley, Maria Cristina" uniqKey="Keightley M" first="Maria Cristina" last="Keightley">Maria Cristina Keightley</name></author><author><name sortKey="Sillekens, Peter" sort="Sillekens, Peter" uniqKey="Sillekens P" first="Peter" last="Sillekens">Peter Sillekens</name></author><author><name sortKey="Schippers, Wim" sort="Schippers, Wim" uniqKey="Schippers W" first="Wim" last="Schippers">Wim Schippers</name></author><author><name sortKey="Rinaldo, Charles" sort="Rinaldo, Charles" uniqKey="Rinaldo C" first="Charles" last="Rinaldo">Charles Rinaldo</name></author><author><name sortKey="George, Kirsten St" sort="George, 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reverse transcription‐PCR</title><author><name sortKey="Keightley, Maria Cristina" sort="Keightley, Maria Cristina" uniqKey="Keightley M" first="Maria Cristina" last="Keightley">Maria Cristina Keightley</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology, University of Pittsburgh, Pittsburgh</wicri:cityArea></affiliation></author><author><name sortKey="Sillekens, Peter" sort="Sillekens, Peter" uniqKey="Sillekens P" first="Peter" last="Sillekens">Peter Sillekens</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of bioMérieux, Boxtel</wicri:regionArea><wicri:noRegion>Boxtel</wicri:noRegion></affiliation></author><author><name sortKey="Schippers, Wim" sort="Schippers, Wim" uniqKey="Schippers W" first="Wim" last="Schippers">Wim Schippers</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of bioMérieux, Boxtel</wicri:regionArea><wicri:noRegion>Boxtel</wicri:noRegion></affiliation></author><author><name sortKey="Rinaldo, Charles" sort="Rinaldo, Charles" uniqKey="Rinaldo C" first="Charles" last="Rinaldo">Charles Rinaldo</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology, University of Pittsburgh, Pittsburgh</wicri:cityArea></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Correspondence address: Clinical Virology, UPMC Presbyterian, A‐912, 200 Lothrop St., Pittsburgh</wicri:cityArea></affiliation></author><author><name sortKey="George, Kirsten St" sort="George, Kirsten St" uniqKey="George K" first="Kirsten St." last="George">Kirsten St. George</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Pennsylvanie</region></placeName><wicri:cityArea>Department of Pathology, University of Pittsburgh, Pittsburgh</wicri:cityArea></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">77</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="602">602</biblScope><biblScope unit="page" to="608">608</biblScope><biblScope unit="page-count">7</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2005-11">2005-11</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Assay</term><term>Chan</term><term>Clin</term><term>Clin microbiol</term><term>Clinical presentation</term><term>Coronavirus</term><term>Dean erdman</term><term>Different combinations</term><term>Dilution series</term><term>Disease control</term><term>Disease onset</term><term>Drosten</term><term>Early detection</term><term>Early diagnosis</term><term>Emery</term><term>European quality assurance</term><term>Genome</term><term>Greater sensitivity</term><term>Greatest sensitivity</term><term>High titer</term><term>Hong kong</term><term>Leader sequence</term><term>Lower case</term><term>Microbiol</term><term>Molecular beacons</term><term>Molecular tests</term><term>Nasba</term><term>Nasba application</term><term>Nasba assay</term><term>Nasba detection</term><term>Novel coronavirus</term><term>Nucleic</term><term>Nucleic acid</term><term>Peiris</term><term>Polymerase</term><term>Polymerase gene</term><term>Primer</term><term>Primer sets</term><term>Respiratory syndrome</term><term>Respiratory syndrome coronavirus</term><term>Respiratory syndrome diagnosis</term><term>Sars</term><term>Sars coronavirus</term><term>Sars patients</term><term>Syndrome</term><term>Taqman</term><term>Taqman sars</term><term>Taqman test</term><term>Urbani</term><term>Urbani strain</term><term>Viral</term><term>Viral load</term><term>Whole genome urbani strain</term><term>World health organization multicentre</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS‐CoV) RNA using real time reverse transcription polymerase chain reaction (RT‐PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real‐time nucleic acid sequence‐based amplification (NASBA) test for SARS‐CoV. A number of primer/beacon sets were designed to target different regions of the SARS‐CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT‐PCR assays. A multi‐target real‐time NASBA application was developed for detection of SARS‐CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real‐time NASBA assay demonstrates equivalent sensitivity when compared to testing by real‐time RT‐PCR. A multi‐target real‐time NASBA assay has been successfully developed for the sensitive detection of SARS‐CoV. J. Med. Virol. 77:602–608, 2005. © 2005 Wiley‐Liss, inc.</div></front></TEI></ISTEX><PubMed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR.</title><author><name sortKey="Keightley, Maria Cristina" sort="Keightley, Maria Cristina" uniqKey="Keightley M" first="Maria Cristina" last="Keightley">Maria Cristina Keightley</name><affiliation wicri:level="1"><nlm:affiliation>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213</wicri:regionArea><wicri:noRegion>Pennsylvania 15213</wicri:noRegion></affiliation></author><author><name sortKey="Sillekens, Peter" sort="Sillekens, Peter" uniqKey="Sillekens P" first="Peter" last="Sillekens">Peter Sillekens</name></author><author><name sortKey="Schippers, Wim" sort="Schippers, Wim" uniqKey="Schippers W" first="Wim" last="Schippers">Wim Schippers</name></author><author><name sortKey="Rinaldo, Charles" sort="Rinaldo, Charles" uniqKey="Rinaldo C" first="Charles" last="Rinaldo">Charles Rinaldo</name></author><author><name sortKey="George, Kirsten St" sort="George, Kirsten St" uniqKey="George K" first="Kirsten St" last="George">Kirsten St George</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16254971</idno><idno type="pmid">16254971</idno><idno type="doi">10.1002/jmv.20498</idno><idno type="wicri:Area/PubMed/Corpus">002473</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002473</idno><idno type="wicri:Area/PubMed/Curation">002473</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002473</idno><idno type="wicri:Area/PubMed/Checkpoint">002547</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002547</idno><idno type="wicri:Area/Ncbi/Merge">001235</idno><idno type="wicri:Area/Ncbi/Curation">001235</idno><idno type="wicri:Area/Ncbi/Checkpoint">001235</idno><idno type="wicri:doubleKey">0146-6615:2005:Keightley M:real:time:nasba</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Real-time NASBA detection of SARS-associated coronavirus and comparison with real-time reverse transcription-PCR.</title><author><name sortKey="Keightley, Maria Cristina" sort="Keightley, Maria Cristina" uniqKey="Keightley M" first="Maria Cristina" last="Keightley">Maria Cristina Keightley</name><affiliation wicri:level="1"><nlm:affiliation>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania 15213</wicri:regionArea><wicri:noRegion>Pennsylvania 15213</wicri:noRegion></affiliation></author><author><name sortKey="Sillekens, Peter" sort="Sillekens, Peter" uniqKey="Sillekens P" first="Peter" last="Sillekens">Peter Sillekens</name></author><author><name sortKey="Schippers, Wim" sort="Schippers, Wim" uniqKey="Schippers W" first="Wim" last="Schippers">Wim Schippers</name></author><author><name sortKey="Rinaldo, Charles" sort="Rinaldo, Charles" uniqKey="Rinaldo C" first="Charles" last="Rinaldo">Charles Rinaldo</name></author><author><name sortKey="George, Kirsten St" sort="George, Kirsten St" uniqKey="George K" first="Kirsten St" last="George">Kirsten St George</name></author></analytic><series><title level="j">Journal of medical virology</title><idno type="ISSN">0146-6615</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term><term>Nucleic Acid Amplification Techniques (methods)</term><term>RNA, Viral (analysis)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (isolation & purification)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>Humains</term><term>RT-PCR</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Techniques d'amplification d'acides nucléiques ()</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Nucleic Acid Amplification Techniques</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>RT-PCR</term><term>Techniques d'amplification d'acides nucléiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS-CoV) RNA using real time reverse transcription polymerase chain reaction (RT-PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real-time nucleic acid sequence-based amplification (NASBA) test for SARS-CoV. A number of primer/beacon sets were designed to target different regions of the SARS-CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT-PCR assays. A multi-target real-time NASBA application was developed for detection of SARS-CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real-time NASBA assay demonstrates equivalent sensitivity when compared to testing by real-time RT-PCR. A multi-target real-time NASBA assay has been successfully developed for the sensitive detection of SARS-CoV.</div></front></TEI></PubMed></double></record>Pour manipuler ce document sous Unix (Dilib)
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