Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR
Identifieur interne : 000394 ( Istex/Corpus ); précédent : 000393; suivant : 000395Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR
Auteurs : Maria Cristina Keightley ; Peter Sillekens ; Wim Schippers ; Charles Rinaldo ; Kirsten St. GeorgeSource :
- Journal of Medical Virology [ 0146-6615 ] ; 2005-11.
English descriptors
- Teeft :
- Assay, Chan, Clin, Clin microbiol, Clinical presentation, Coronavirus, Dean erdman, Different combinations, Dilution series, Disease control, Disease onset, Drosten, Early detection, Early diagnosis, Emery, European quality assurance, Genome, Greater sensitivity, Greatest sensitivity, High titer, Hong kong, Leader sequence, Lower case, Microbiol, Molecular beacons, Molecular tests, Nasba, Nasba application, Nasba assay, Nasba detection, Novel coronavirus, Nucleic, Nucleic acid, Peiris, Polymerase, Polymerase gene, Primer, Primer sets, Respiratory syndrome, Respiratory syndrome coronavirus, Respiratory syndrome diagnosis, Sars, Sars coronavirus, Sars patients, Syndrome, Taqman, Taqman sars, Taqman test, Urbani, Urbani strain, Viral, Viral load, Whole genome urbani strain, World health organization multicentre.
Abstract
Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS‐CoV) RNA using real time reverse transcription polymerase chain reaction (RT‐PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real‐time nucleic acid sequence‐based amplification (NASBA) test for SARS‐CoV. A number of primer/beacon sets were designed to target different regions of the SARS‐CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT‐PCR assays. A multi‐target real‐time NASBA application was developed for detection of SARS‐CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real‐time NASBA assay demonstrates equivalent sensitivity when compared to testing by real‐time RT‐PCR. A multi‐target real‐time NASBA assay has been successfully developed for the sensitive detection of SARS‐CoV. J. Med. Virol. 77:602–608, 2005. © 2005 Wiley‐Liss, inc.
Url:
DOI: 10.1002/jmv.20498
Links to Exploration step
ISTEX:098DFCB2D53357E19C5E683F42475B33A4D27C1ELe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR</title><author><name sortKey="Keightley, Maria Cristina" sort="Keightley, Maria Cristina" uniqKey="Keightley M" first="Maria Cristina" last="Keightley">Maria Cristina Keightley</name><affiliation><mods:affiliation>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</mods:affiliation></affiliation></author><author><name sortKey="Sillekens, Peter" sort="Sillekens, Peter" uniqKey="Sillekens P" first="Peter" last="Sillekens">Peter Sillekens</name><affiliation><mods:affiliation>Department of bioMérieux, Boxtel, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Schippers, Wim" sort="Schippers, Wim" uniqKey="Schippers W" first="Wim" last="Schippers">Wim Schippers</name><affiliation><mods:affiliation>Department of bioMérieux, Boxtel, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Rinaldo, Charles" sort="Rinaldo, Charles" uniqKey="Rinaldo C" first="Charles" last="Rinaldo">Charles Rinaldo</name><affiliation><mods:affiliation>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: rinaldocr@upmc.edu</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Clinical Virology, UPMC Presbyterian, A‐912, 200 Lothrop St., Pittsburgh, PA 15213.===</mods:affiliation></affiliation></author><author><name sortKey="George, Kirsten St" sort="George, Kirsten St" uniqKey="George K" first="Kirsten St." last="George">Kirsten St. George</name><affiliation><mods:affiliation>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</mods:affiliation></affiliation><affiliation><mods:affiliation>Current Address: Wadsworth Center, New York State Department of Health, Albany, NY.</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:098DFCB2D53357E19C5E683F42475B33A4D27C1E</idno><date when="2005" year="2005">2005</date><idno type="doi">10.1002/jmv.20498</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-ZD79ZC6B-F/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000394</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000394</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR</title><author><name sortKey="Keightley, Maria Cristina" sort="Keightley, Maria Cristina" uniqKey="Keightley M" first="Maria Cristina" last="Keightley">Maria Cristina Keightley</name><affiliation><mods:affiliation>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</mods:affiliation></affiliation></author><author><name sortKey="Sillekens, Peter" sort="Sillekens, Peter" uniqKey="Sillekens P" first="Peter" last="Sillekens">Peter Sillekens</name><affiliation><mods:affiliation>Department of bioMérieux, Boxtel, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Schippers, Wim" sort="Schippers, Wim" uniqKey="Schippers W" first="Wim" last="Schippers">Wim Schippers</name><affiliation><mods:affiliation>Department of bioMérieux, Boxtel, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Rinaldo, Charles" sort="Rinaldo, Charles" uniqKey="Rinaldo C" first="Charles" last="Rinaldo">Charles Rinaldo</name><affiliation><mods:affiliation>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: rinaldocr@upmc.edu</mods:affiliation></affiliation><affiliation><mods:affiliation>Correspondence address: Clinical Virology, UPMC Presbyterian, A‐912, 200 Lothrop St., Pittsburgh, PA 15213.===</mods:affiliation></affiliation></author><author><name sortKey="George, Kirsten St" sort="George, Kirsten St" uniqKey="George K" first="Kirsten St." last="George">Kirsten St. George</name><affiliation><mods:affiliation>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</mods:affiliation></affiliation><affiliation><mods:affiliation>Current Address: Wadsworth Center, New York State Department of Health, Albany, NY.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><biblScope unit="vol">77</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="602">602</biblScope><biblScope unit="page" to="608">608</biblScope><biblScope unit="page-count">7</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2005-11">2005-11</date></imprint><idno type="ISSN">0146-6615</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Assay</term><term>Chan</term><term>Clin</term><term>Clin microbiol</term><term>Clinical presentation</term><term>Coronavirus</term><term>Dean erdman</term><term>Different combinations</term><term>Dilution series</term><term>Disease control</term><term>Disease onset</term><term>Drosten</term><term>Early detection</term><term>Early diagnosis</term><term>Emery</term><term>European quality assurance</term><term>Genome</term><term>Greater sensitivity</term><term>Greatest sensitivity</term><term>High titer</term><term>Hong kong</term><term>Leader sequence</term><term>Lower case</term><term>Microbiol</term><term>Molecular beacons</term><term>Molecular tests</term><term>Nasba</term><term>Nasba application</term><term>Nasba assay</term><term>Nasba detection</term><term>Novel coronavirus</term><term>Nucleic</term><term>Nucleic acid</term><term>Peiris</term><term>Polymerase</term><term>Polymerase gene</term><term>Primer</term><term>Primer sets</term><term>Respiratory syndrome</term><term>Respiratory syndrome coronavirus</term><term>Respiratory syndrome diagnosis</term><term>Sars</term><term>Sars coronavirus</term><term>Sars patients</term><term>Syndrome</term><term>Taqman</term><term>Taqman sars</term><term>Taqman test</term><term>Urbani</term><term>Urbani strain</term><term>Viral</term><term>Viral load</term><term>Whole genome urbani strain</term><term>World health organization multicentre</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS‐CoV) RNA using real time reverse transcription polymerase chain reaction (RT‐PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real‐time nucleic acid sequence‐based amplification (NASBA) test for SARS‐CoV. A number of primer/beacon sets were designed to target different regions of the SARS‐CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT‐PCR assays. A multi‐target real‐time NASBA application was developed for detection of SARS‐CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real‐time NASBA assay demonstrates equivalent sensitivity when compared to testing by real‐time RT‐PCR. A multi‐target real‐time NASBA assay has been successfully developed for the sensitive detection of SARS‐CoV. J. Med. Virol. 77:602–608, 2005. © 2005 Wiley‐Liss, inc.</div></front></TEI><istex><corpusName>wiley</corpusName><keywords><teeft><json:string>nasba</json:string><json:string>sars</json:string><json:string>primer</json:string><json:string>coronavirus</json:string><json:string>assay</json:string><json:string>respiratory syndrome</json:string><json:string>taqman</json:string><json:string>clin</json:string><json:string>urbani</json:string><json:string>emery</json:string><json:string>drosten</json:string><json:string>chan</json:string><json:string>genome</json:string><json:string>polymerase</json:string><json:string>peiris</json:string><json:string>microbiol</json:string><json:string>viral</json:string><json:string>novel coronavirus</json:string><json:string>nasba assay</json:string><json:string>syndrome</json:string><json:string>sars coronavirus</json:string><json:string>early detection</json:string><json:string>dilution series</json:string><json:string>nucleic</json:string><json:string>nucleic acid</json:string><json:string>viral load</json:string><json:string>respiratory syndrome diagnosis</json:string><json:string>nasba detection</json:string><json:string>world health organization multicentre</json:string><json:string>urbani strain</json:string><json:string>dean erdman</json:string><json:string>early diagnosis</json:string><json:string>molecular beacons</json:string><json:string>taqman sars</json:string><json:string>nasba application</json:string><json:string>different combinations</json:string><json:string>european quality assurance</json:string><json:string>lower case</json:string><json:string>clinical presentation</json:string><json:string>whole genome urbani strain</json:string><json:string>hong kong</json:string><json:string>primer sets</json:string><json:string>leader sequence</json:string><json:string>polymerase gene</json:string><json:string>taqman test</json:string><json:string>greater sensitivity</json:string><json:string>sars patients</json:string><json:string>molecular tests</json:string><json:string>disease onset</json:string><json:string>respiratory syndrome coronavirus</json:string><json:string>greatest sensitivity</json:string><json:string>disease control</json:string><json:string>clin microbiol</json:string><json:string>high titer</json:string></teeft></keywords><author><json:item><name>Maria Cristina Keightley</name><affiliations><json:string>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</json:string><json:string>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</json:string></affiliations></json:item><json:item><name>Peter Sillekens</name><affiliations><json:string>Department of bioMérieux, Boxtel, The Netherlands</json:string></affiliations></json:item><json:item><name>Wim Schippers</name><affiliations><json:string>Department of bioMérieux, Boxtel, The Netherlands</json:string></affiliations></json:item><json:item><name>Charles Rinaldo</name><affiliations><json:string>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</json:string><json:string>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</json:string><json:string>E-mail: rinaldocr@upmc.edu</json:string><json:string>Correspondence address: Clinical Virology, UPMC Presbyterian, A‐912, 200 Lothrop St., Pittsburgh, PA 15213.===</json:string></affiliations></json:item><json:item><name>Kirsten St. George</name><affiliations><json:string>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</json:string><json:string>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</json:string><json:string>Current Address: Wadsworth Center, New York State Department of Health, Albany, NY.</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>SARS</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>NASBA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>RT‐PCR</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>coronavirus</value></json:item></subject><articleId><json:string>JMV20498</json:string></articleId><arkIstex>ark:/67375/WNG-ZD79ZC6B-F</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS‐CoV) RNA using real time reverse transcription polymerase chain reaction (RT‐PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real‐time nucleic acid sequence‐based amplification (NASBA) test for SARS‐CoV. A number of primer/beacon sets were designed to target different regions of the SARS‐CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT‐PCR assays. A multi‐target real‐time NASBA application was developed for detection of SARS‐CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real‐time NASBA assay demonstrates equivalent sensitivity when compared to testing by real‐time RT‐PCR. A multi‐target real‐time NASBA assay has been successfully developed for the sensitive detection of SARS‐CoV. J. Med. Virol. 77:602–608, 2005. © 2005 Wiley‐Liss, inc.</abstract><qualityIndicators><score>8.4</score><pdfWordCount>4012</pdfWordCount><pdfCharCount>25725</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>7</pdfPageCount><pdfPageSize>594 x 792 pts</pdfPageSize><pdfWordsPerPage>573</pdfWordsPerPage><pdfText>true</pdfText><refBibsNative>true</refBibsNative><abstractWordCount>199</abstractWordCount><abstractCharCount>1398</abstractCharCount><keywordCount>4</keywordCount></qualityIndicators><title>Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR</title><pmid><json:string>16254971</json:string></pmid><genre><json:string>article</json:string></genre><host><title>Journal of Medical Virology</title><language><json:string>unknown</json:string></language><doi><json:string>10.1002/(ISSN)1096-9071</json:string></doi><issn><json:string>0146-6615</json:string></issn><eissn><json:string>1096-9071</json:string></eissn><publisherId><json:string>JMV</json:string></publisherId><volume>77</volume><issue>4</issue><pages><first>602</first><last>608</last><total>7</total></pages><genre><json:string>journal</json:string></genre><subject><json:item><value>Research Article</value></json:item></subject></host><namedEntities><unitex><date><json:string>2005</json:string></date><geogName></geogName><orgName><json:string>World Health Organization Multicentre Collaborative Network for Severe Acute Respiratory Syndrome Diagnosis</json:string><json:string>Bernhard-Nocht Institute for Tropical Medicine</json:string><json:string>BNITM</json:string><json:string>Netherlands Department</json:string><json:string>Wiley-Liss, Inc</json:string><json:string>LISS, INC.</json:string><json:string>University of Pittsburgh Medical Center</json:string><json:string>American Type Culture Collection</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Peter Sillekens</json:string><json:string>Hong Kong</json:string><json:string>J. Med</json:string><json:string>Molecular</json:string><json:string>Kirsten St. George</json:string><json:string>Dean Erdman</json:string><json:string>Data</json:string><json:string>Charles Rinaldo</json:string><json:string>Isolates</json:string><json:string>I. Primers</json:string><json:string>Christian Drosten</json:string></persName><placeName><json:string>Durham</json:string><json:string>Foster City</json:string><json:string>Germany</json:string><json:string>Germantown</json:string><json:string>GA</json:string><json:string>Manassas</json:string><json:string>VA</json:string><json:string>Hamburg</json:string><json:string>NC</json:string><json:string>Atlanta</json:string><json:string>MD</json:string><json:string>Frankfurt</json:string><json:string>Rotterdam</json:string><json:string>CA</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Rest and Mindell, 2003</json:string><json:string>Poon et al., 2004</json:string><json:string>Ksiazek et al., 2003</json:string><json:string>Ginocchio et al., 2003</json:string><json:string>Brown and Tetro, 2003</json:string><json:string>Drosten et al., 2003</json:string><json:string>Emery et al., 2004</json:string><json:string>Ruan et al. [2003]</json:string><json:string>Fouchier et al., 2003</json:string><json:string>November, 2002</json:string><json:string>Bressler and Nolte, 2004</json:string><json:string>Lanciotti and Kerst, 2001</json:string><json:string>Chen et al., 2004</json:string><json:string>Peiris et al., 2003</json:string><json:string>Yam et al., 2003</json:string><json:string>Marra et al., 2003</json:string><json:string>Lau et al., 2004</json:string><json:string>Jiang et al., 2004</json:string><json:string>World Health Organization Multicentre Collaborative Network for Severe Acute Respiratory Syndrome Diagnosis, 2003</json:string><json:string>Keightley et al.</json:string><json:string>Zhang et al., 2004</json:string><json:string>Mahony et al., 2004</json:string><json:string>Drosten et al., 2004</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/WNG-ZD79ZC6B-F</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Infectious Diseases</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - invertebres</json:string></inist></categories><publicationDate>2005</publicationDate><copyrightDate>2005</copyrightDate><doi><json:string>10.1002/jmv.20498</json:string></doi><id>098DFCB2D53357E19C5E683F42475B33A4D27C1E</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-ZD79ZC6B-F/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-ZD79ZC6B-F/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/WNG-ZD79ZC6B-F/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main">Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR</title><title level="a" type="short" xml:lang="en">Real‐Time NASBA for SARS</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><availability><licence>Copyright © 2005 Wiley‐Liss, Inc.</licence></availability><date type="published" when="2005-11"></date></publicationStmt><notesStmt><note type="content-type" subtype="article" source="article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</note><note type="publication-type" subtype="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="article"><analytic><title level="a" type="main">Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR</title><title level="a" type="short" xml:lang="en">Real‐Time NASBA for SARS</title><author xml:id="author-0000"><persName><forename type="first">Maria Cristina</forename><surname>Keightley</surname></persName><affiliation><orgName type="laboratory">Clinical Virology Laboratory</orgName><orgName type="institution">University of Pittsburgh Medical Center</orgName><address><addrLine>Pittsburgh</addrLine><addrLine>Pennsylvania</addrLine><country key="US" xml:lang="en">UNITED STATES</country></address></affiliation><affiliation><orgName type="department">Department of Pathology</orgName><orgName type="institution">University of Pittsburgh</orgName><address><addrLine>Pittsburgh</addrLine><addrLine>Pennsylvania</addrLine><country key="US" xml:lang="en">UNITED STATES</country></address></affiliation></author><author xml:id="author-0001"><persName><forename type="first">Peter</forename><surname>Sillekens</surname></persName><affiliation><orgName type="division">Department of bioMérieux</orgName><address><addrLine>Boxtel</addrLine><addrLine>The Netherlands</addrLine><country key="NL" xml:lang="en">THE NETHERLANDS</country></address></affiliation></author><author xml:id="author-0002"><persName><forename type="first">Wim</forename><surname>Schippers</surname></persName><affiliation><orgName type="division">Department of bioMérieux</orgName><address><addrLine>Boxtel</addrLine><addrLine>The Netherlands</addrLine><country key="NL" xml:lang="en">THE NETHERLANDS</country></address></affiliation></author><author xml:id="author-0003" role="corresp"><persName><forename type="first">Charles</forename><surname>Rinaldo</surname></persName><email>rinaldocr@upmc.edu</email><affiliation><orgName type="laboratory">Clinical Virology Laboratory</orgName><orgName type="institution">University of Pittsburgh Medical Center</orgName><address><addrLine>Pittsburgh</addrLine><addrLine>Pennsylvania</addrLine><country key="US" xml:lang="en">UNITED STATES</country></address></affiliation><affiliation><orgName type="department">Department of Pathology</orgName><orgName type="institution">University of Pittsburgh</orgName><address><addrLine>Pittsburgh</addrLine><addrLine>Pennsylvania</addrLine><country key="US" xml:lang="en">UNITED STATES</country></address></affiliation></author><author xml:id="author-0004"><persName><forename type="first">Kirsten St.</forename><surname>George</surname></persName><affiliation><orgName type="laboratory">Clinical Virology Laboratory</orgName><orgName type="institution">University of Pittsburgh Medical Center</orgName><address><addrLine>Pittsburgh</addrLine><addrLine>Pennsylvania</addrLine><country key="US" xml:lang="en">UNITED STATES</country></address></affiliation><affiliation><orgName type="department">Department of Pathology</orgName><orgName type="institution">University of Pittsburgh</orgName><address><addrLine>Pittsburgh</addrLine><addrLine>Pennsylvania</addrLine><country key="US" xml:lang="en">UNITED STATES</country></address></affiliation><affiliation><orgName type="institution">Wadsworth Center</orgName><orgName type="division">New York State Department of Health</orgName><address><addrLine>Albany</addrLine><addrLine>NY.</addrLine><country key="US" xml:lang="en">UNITED STATES</country></address></affiliation></author><idno type="istex">098DFCB2D53357E19C5E683F42475B33A4D27C1E</idno><idno type="ark">ark:/67375/WNG-ZD79ZC6B-F</idno><idno type="DOI">10.1002/jmv.20498</idno><idno type="unit">JMV20498</idno><idno type="toTypesetVersion">file:JMV.JMV20498.pdf</idno></analytic><monogr><title level="j" type="main">Journal of Medical Virology</title><title level="j" type="alt">JOURNAL OF MEDICAL VIROLOGY</title><idno type="pISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><idno type="book-DOI">10.1002/(ISSN)1096-9071</idno><idno type="book-part-DOI">10.1002/jmv.v77:4</idno><idno type="product">JMV</idno><imprint><biblScope unit="vol">77</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="602">602</biblScope><biblScope unit="page" to="608">608</biblScope><biblScope unit="page-count">7</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2005-11"></date></imprint></monogr></biblStruct></sourceDesc></fileDesc><encodingDesc><schemaRef type="ODD" url="https://xml-schema.delivery.istex.fr/tei-istex.odd"></schemaRef><appInfo><application ident="pub2tei" version="1.0.10" when="2019-12-20"><label>pub2TEI-ISTEX</label><desc>A set of style sheets for converting XML documents encoded in various scientific publisher formats into a common TEI format.
<ref target="http://www.tei-c.org/">We use TEI</ref></desc></application></appInfo></encodingDesc><profileDesc><abstract xml:lang="en" style="main"><head>Abstract</head><p>Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS‐CoV) RNA using real time reverse transcription polymerase chain reaction (RT‐PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real‐time nucleic acid sequence‐based amplification (NASBA) test for SARS‐CoV. A number of primer/beacon sets were designed to target different regions of the SARS‐CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT‐PCR assays. A multi‐target real‐time NASBA application was developed for detection of SARS‐CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real‐time NASBA assay demonstrates equivalent sensitivity when compared to testing by real‐time RT‐PCR. A multi‐target real‐time NASBA assay has been successfully developed for the sensitive detection of SARS‐CoV. <hi rend="italic">J. Med. Virol.</hi> 77:602–608, 2005. © 2005 Wiley‐Liss, inc.</p></abstract><textClass><keywords xml:lang="en"><term xml:id="kwd1">SARS</term><term xml:id="kwd2">NASBA</term><term xml:id="kwd3">RT‐PCR</term><term xml:id="kwd4">coronavirus</term></keywords><keywords rend="articleCategory"><term>Research Article</term></keywords></textClass><langUsage><language ident="en"></language></langUsage></profileDesc><revisionDesc><change when="2019-12-20" who="#istex" xml:id="pub2tei">formatting</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-ZD79ZC6B-F/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Subscription Services, Inc., A Wiley Company</publisherName><publisherLoc>Hoboken</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1096-9071</doi><issn type="print">0146-6615</issn><issn type="electronic">1096-9071</issn><idGroup><id type="product" value="JMV"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="JOURNAL OF MEDICAL VIROLOGY">Journal of Medical Virology</title><title type="short">J. Med. Virol.</title></titleGroup></publicationMeta><publicationMeta level="part" position="40"><doi origin="wiley" registered="yes">10.1002/jmv.v77:4</doi><numberingGroup><numbering type="journalVolume" number="77">77</numbering><numbering type="journalIssue">4</numbering></numberingGroup><coverDate startDate="2005-11">November 2005</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="230" status="forIssue"><doi origin="wiley" registered="yes">10.1002/jmv.20498</doi><idGroup><id type="unit" value="JMV20498"></id></idGroup><countGroup><count type="pageTotal" number="7"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title></titleGroup><copyright ownership="publisher">Copyright © 2005 Wiley‐Liss, Inc.</copyright><eventGroup><event type="manuscriptAccepted" date="2005-08-19"></event><event type="firstOnline" date="2005-10-27"></event><event type="publishedOnlineFinalForm" date="2005-10-27"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-15"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-20"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst">602</numbering><numbering type="pageLast">608</numbering></numberingGroup><correspondenceTo>Clinical Virology, UPMC Presbyterian, A‐912, 200 Lothrop St., Pittsburgh, PA 15213.===</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JMV.JMV20498.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="2"></count><count type="tableTotal" number="4"></count><count type="referenceTotal" number="25"></count><count type="wordTotal" number="1144"></count></countGroup><titleGroup><title type="main" xml:lang="en">Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR</title><title type="short" xml:lang="en">Real‐Time NASBA for SARS</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1 #af2"><personName><givenNames>Maria Cristina</givenNames><familyName>Keightley</familyName></personName></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Peter</givenNames><familyName>Sillekens</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af3"><personName><givenNames>Wim</givenNames><familyName>Schippers</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af1 #af2" corresponding="yes"><personName><givenNames>Charles</givenNames><familyName>Rinaldo</familyName></personName><contactDetails><email>rinaldocr@upmc.edu</email></contactDetails></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af1 #af2" currentRef="#curr1"><personName><givenNames>Kirsten St.</givenNames><familyName>George</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="US" type="organization"><unparsedAffiliation>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="US" type="organization"><unparsedAffiliation>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="NL" type="organization"><unparsedAffiliation>Department of bioMérieux, Boxtel, The Netherlands</unparsedAffiliation></affiliation><affiliation xml:id="curr1" countryCode="US"><unparsedAffiliation>Wadsworth Center, New York State Department of Health, Albany, NY.</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">SARS</keyword><keyword xml:id="kwd2">NASBA</keyword><keyword xml:id="kwd3">RT‐PCR</keyword><keyword xml:id="kwd4">coronavirus</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS‐CoV) RNA using real time reverse transcription polymerase chain reaction (RT‐PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real‐time nucleic acid sequence‐based amplification (NASBA) test for SARS‐CoV. A number of primer/beacon sets were designed to target different regions of the SARS‐CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT‐PCR assays. A multi‐target real‐time NASBA application was developed for detection of SARS‐CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real‐time NASBA assay demonstrates equivalent sensitivity when compared to testing by real‐time RT‐PCR. A multi‐target real‐time NASBA assay has been successfully developed for the sensitive detection of SARS‐CoV. <i>J. Med. Virol.</i> 77:602–608, 2005. © 2005 Wiley‐Liss, inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Real‐Time NASBA for SARS</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR</title></titleInfo><name type="personal"><namePart type="given">Maria Cristina</namePart><namePart type="family">Keightley</namePart><affiliation>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</affiliation><affiliation>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Peter</namePart><namePart type="family">Sillekens</namePart><affiliation>Department of bioMérieux, Boxtel, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wim</namePart><namePart type="family">Schippers</namePart><affiliation>Department of bioMérieux, Boxtel, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Charles</namePart><namePart type="family">Rinaldo</namePart><affiliation>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</affiliation><affiliation>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</affiliation><affiliation>E-mail: rinaldocr@upmc.edu</affiliation><affiliation>Correspondence address: Clinical Virology, UPMC Presbyterian, A‐912, 200 Lothrop St., Pittsburgh, PA 15213.===</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kirsten St.</namePart><namePart type="family">George</namePart><affiliation>Clinical Virology Laboratory, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania</affiliation><affiliation>Department of Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania</affiliation><affiliation>Current Address: Wadsworth Center, New York State Department of Health, Albany, NY.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-6N5SZHKN-D">article</genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2005-11</dateIssued><dateValid encoding="w3cdtf">2005-08-19</dateValid><copyrightDate encoding="w3cdtf">2005</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><extent unit="figures">2</extent><extent unit="tables">4</extent><extent unit="references">25</extent><extent unit="words">1144</extent></physicalDescription><abstract lang="en">Severe acute respiratory syndrome (SARS) exhibits a high mortality rate and the potential for rapid epidemic spread. Additionally, it has a poorly defined clinical presentation, and no known treatment or prevention methods. Collectively, these factors underscore the need for early diagnosis. Molecular tests have been developed to detect SARS coronavirus (SARS‐CoV) RNA using real time reverse transcription polymerase chain reaction (RT‐PCR) with varying levels of sensitivity. However, RNA amplification methods have been demonstrated to be more sensitive for the detection of some RNA viruses. We therefore developed a real‐time nucleic acid sequence‐based amplification (NASBA) test for SARS‐CoV. A number of primer/beacon sets were designed to target different regions of the SARS‐CoV genome, and were tested for sensitivity and specificity. The performance of the assays was compared with RT‐PCR assays. A multi‐target real‐time NASBA application was developed for detection of SARS‐CoV polymerase (Pol) and nucleocapsid (N) genes. The N targets were found to be consistently more sensitive than the Pol targets, and the real‐time NASBA assay demonstrates equivalent sensitivity when compared to testing by real‐time RT‐PCR. A multi‐target real‐time NASBA assay has been successfully developed for the sensitive detection of SARS‐CoV. J. Med. Virol. 77:602–608, 2005. © 2005 Wiley‐Liss, inc.</abstract><subject lang="en"><genre>keywords</genre><topic>SARS</topic><topic>NASBA</topic><topic>RT‐PCR</topic><topic>coronavirus</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Medical Virology</title></titleInfo><titleInfo type="abbreviated"><title>J. Med. Virol.</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">0146-6615</identifier><identifier type="eISSN">1096-9071</identifier><identifier type="DOI">10.1002/(ISSN)1096-9071</identifier><identifier type="PublisherID">JMV</identifier><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>77</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>602</start><end>608</end><total>7</total></extent></part></relatedItem><relatedItem type="references" displayLabel="cit1"><titleInfo><title>Preclinical evaluation of two real‐time, reverse transcription‐PCR assays for detection of the severe acute respiratory syndrome coronavirus</title></titleInfo><name type="personal"><namePart type="given">AM</namePart><namePart type="family">Bressler</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">FS</namePart><namePart type="family">Nolte</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Bressler AM, Nolte FS. 2004. Preclinical evaluation of two real‐time, reverse transcription‐PCR assays for detection of the severe acute respiratory syndrome coronavirus. J Clin Microbiol 42: 987–991.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>42</number></detail><extent unit="pages"><start>987</start><end>991</end></extent></part><relatedItem type="host"><titleInfo><title>J Clin Microbiol</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>42</number></detail><extent unit="pages"><start>987</start><end>991</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit2"><titleInfo><title>Comparative analysis of the SARS coronavirus genome: A good start to a long journey</title></titleInfo><name type="personal"><namePart type="given">EG</namePart><namePart type="family">Brown</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JA</namePart><namePart type="family">Tetro</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Brown EG, Tetro JA. 2003. Comparative analysis of the SARS coronavirus genome: A good start to a long journey. Lancet 361: 1756–1757.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>361</number></detail><extent unit="pages"><start>1756</start><end>1757</end></extent></part><relatedItem type="host"><titleInfo><title>Lancet</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>361</number></detail><extent unit="pages"><start>1756</start><end>1757</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit3"><titleInfo><title>Human metapneumovirus detection in patients with severe acute respiratory syndrome</title></titleInfo><name type="personal"><namePart type="given">PK</namePart><namePart type="family">Chan</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Chan PK. 2003. Human metapneumovirus detection in patients with severe acute respiratory syndrome. Emerg Infect Dis 9: 1058–1063.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>9</number></detail><extent unit="pages"><start>1058</start><end>1063</end></extent></part><relatedItem type="host"><titleInfo><title>Emerg Infect Dis</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>9</number></detail><extent unit="pages"><start>1058</start><end>1063</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit4"><titleInfo><title>Serology of severe acute respiratory syndrome: Implications for surveillance and outcome</title></titleInfo><name type="personal"><namePart type="given">X</namePart><namePart type="family">Chen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B</namePart><namePart type="family">Zhou</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Li</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">X</namePart><namePart type="family">Liang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H</namePart><namePart type="family">Wang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G</namePart><namePart type="family">Yang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H</namePart><namePart type="family">Wang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">X</namePart><namePart type="family">Le</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Chen X, Zhou B, Li M, Liang X, Wang H, Yang G, Wang H, Le X. 2004. Serology of severe acute respiratory syndrome: Implications for surveillance and outcome. J Infect Dis 189: 1158–1163.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>189</number></detail><extent unit="pages"><start>1158</start><end>1163</end></extent></part><relatedItem type="host"><titleInfo><title>J Infect Dis</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>189</number></detail><extent unit="pages"><start>1158</start><end>1163</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit5"><titleInfo><title>Identification of a novel coronavirus in patients with severe acute respiratory syndrome</title></titleInfo><name type="personal"><namePart type="given">C</namePart><namePart type="family">Drosten</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Gunther</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W</namePart><namePart type="family">Preiser</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">WS</namePart><namePart type="family">van der</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">HR</namePart><namePart type="family">Brodt</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Becker</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H</namePart><namePart type="family">Rabenau</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Panning</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L</namePart><namePart type="family">Kolesnikova</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">RA</namePart><namePart type="family">Fouchier</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Berger</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AM</namePart><namePart type="family">Burguiere</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J</namePart><namePart type="family">Cinatl</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Eickmann</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N</namePart><namePart type="family">Escriou</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K</namePart><namePart type="family">Grywna</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Kramme</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JC</namePart><namePart type="family">Manuguerra</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Muller</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">V</namePart><namePart type="family">Rickerts</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Sturmer</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Vieth</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">HD</namePart><namePart type="family">Klenk</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AD</namePart><namePart type="family">Osterhaus</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H</namePart><namePart type="family">Schmitz</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">HW</namePart><namePart type="family">Doerr</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Drosten C, Gunther S, Preiser W, van der WS, Brodt HR, Becker S, Rabenau H, Panning M, Kolesnikova L, Fouchier RA, Berger A, Burguiere AM, Cinatl J, Eickmann M, Escriou N, Grywna K, Kramme S, Manuguerra JC, Muller S, Rickerts V, Sturmer M, Vieth S, Klenk HD, Osterhaus AD, Schmitz H, Doerr HW. 2003. Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Engl J Med 348: 1967–1976.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>348</number></detail><extent unit="pages"><start>1967</start><end>1976</end></extent></part><relatedItem type="host"><titleInfo><title>N Engl J Med</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>348</number></detail><extent unit="pages"><start>1967</start><end>1976</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit6"><titleInfo><title>Evaluation of advanced reverse transcription‐PCR assays and an alternative PCR target region for detection of severe acute respiratory syndrome‐associated coronavirus</title></titleInfo><name type="personal"><namePart type="given">C</namePart><namePart type="family">Drosten</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L‐L</namePart><namePart type="family">Chiu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Panning</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">HN</namePart><namePart type="family">Leong</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W</namePart><namePart type="family">Preiser</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JS</namePart><namePart type="family">Tam</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Gunther</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Kramme</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P</namePart><namePart type="family">Emmerich</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">WL</namePart><namePart type="family">Ng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H</namePart><namePart type="family">Schmitz</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">ESC</namePart><namePart type="family">Koay</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Drosten C, Chiu L‐L, Panning M, Leong HN, Preiser W, Tam JS, Gunther S, Kramme S, Emmerich P, Ng WL, Schmitz H, Koay ESC. 2004. Evaluation of advanced reverse transcription‐PCR assays and an alternative PCR target region for detection of severe acute respiratory syndrome‐associated coronavirus. J Clin Microbiol 42: 2043–2047.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>42</number></detail><extent unit="pages"><start>2043</start><end>2047</end></extent></part><relatedItem type="host"><titleInfo><title>J Clin Microbiol</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>42</number></detail><extent unit="pages"><start>2043</start><end>2047</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit7"><titleInfo><title>Real‐time reverse transcription‐polymerase chain reaction assay for SARS‐associated coronavirus</title></titleInfo><name type="personal"><namePart type="given">SL</namePart><namePart type="family">Emery</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">DD</namePart><namePart type="family">Erdman</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">MD</namePart><namePart type="family">Bowen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">BR</namePart><namePart type="family">Newton</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JM</namePart><namePart type="family">Winchell</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">RF</namePart><namePart type="family">Meyer</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Tong</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">BT</namePart><namePart type="family">Cook</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">BP</namePart><namePart type="family">Holloway</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KA</namePart><namePart type="family">McCaustland</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">PA</namePart><namePart type="family">Rota</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B</namePart><namePart type="family">Benkamp</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">LE</namePart><namePart type="family">Lowe</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">TG</namePart><namePart type="family">Ksiazek</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">WJ</namePart><namePart type="family">Bellini</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">LJ</namePart><namePart type="family">Anderson</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Emery SL, Erdman DD, Bowen MD, Newton BR, Winchell JM, Meyer RF, Tong S, Cook BT, Holloway BP, McCaustland KA, Rota PA, Benkamp B, Lowe LE, Ksiazek TG, Bellini WJ, Anderson LJ. 2004. Real‐time reverse transcription‐polymerase chain reaction assay for SARS‐associated coronavirus. Emerg Infect Dis 10: 311–316.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>10</number></detail><extent unit="pages"><start>311</start><end>316</end></extent></part><relatedItem type="host"><titleInfo><title>Emerg Infect Dis</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>10</number></detail><extent unit="pages"><start>311</start><end>316</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit8"><titleInfo><title>Aetiology: Koch's postulates fulfilled for SARS virus</title></titleInfo><name type="personal"><namePart type="given">RA</namePart><namePart type="family">Fouchier</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T</namePart><namePart type="family">Kuiken</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Schutten</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G</namePart><namePart type="family">van Amerongen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">GJ</namePart><namePart type="family">van Doornum</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">BG</namePart><namePart type="family">van den Hoogen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Peiris</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W</namePart><namePart type="family">Lim</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K</namePart><namePart type="family">Stohr</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AD</namePart><namePart type="family">Osterhaus</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Fouchier RA, Kuiken T, Schutten M, van Amerongen G, van Doornum GJ, van den Hoogen BG, Peiris M, Lim W, Stohr K, Osterhaus AD. 2003. Aetiology: Koch's postulates fulfilled for SARS virus. Nature 423: 240.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>423</number></detail><extent unit="pages"><start>240</start></extent></part><relatedItem type="host"><titleInfo><title>Nature</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>423</number></detail><extent unit="pages"><start>240</start></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit9"><titleInfo><title>Multicenter evaluation of the performance characteristics of the NucliSens HIV‐1 QT assay used for quantitation of human immunodeficiency virus type 1 RNA</title></titleInfo><name type="personal"><namePart type="given">CC</namePart><namePart type="family">Ginocchio</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Kemper</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KA</namePart><namePart type="family">Stellrecht</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">DJ</namePart><namePart type="family">Witt</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Ginocchio CC, Kemper M, Stellrecht KA, Witt DJ. 2003. Multicenter evaluation of the performance characteristics of the NucliSens HIV‐1 QT assay used for quantitation of human immunodeficiency virus type 1 RNA. J Clin Microbiol 41: 164–173.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>41</number></detail><extent unit="pages"><start>164</start><end>173</end></extent></part><relatedItem type="host"><titleInfo><title>J Clin Microbiol</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>41</number></detail><extent unit="pages"><start>164</start><end>173</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit10"><titleInfo><title>Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real‐time nested polymerase chain reaction</title></titleInfo><name type="personal"><namePart type="given">SS</namePart><namePart type="family">Jiang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">TC</namePart><namePart type="family">Chen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JY</namePart><namePart type="family">Yang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">CA</namePart><namePart type="family">Hsiung</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">IJ</namePart><namePart type="family">Su</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">YL</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">PC</namePart><namePart type="family">Chen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JL</namePart><namePart type="family">Juang</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Jiang SS, Chen TC, Yang JY, Hsiung CA, Su IJ, Liu YL, Chen PC, Juang JL. 2004. Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real‐time nested polymerase chain reaction. Clin Infect Dis 38: 293–296.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>38</number></detail><extent unit="pages"><start>293</start><end>296</end></extent></part><relatedItem type="host"><titleInfo><title>Clin Infect Dis</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>38</number></detail><extent unit="pages"><start>293</start><end>296</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit11"><titleInfo><title>A novel coronavirus associated with severe acute respiratory syndrome</title></titleInfo><name type="personal"><namePart type="given">TG</namePart><namePart type="family">Ksiazek</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D</namePart><namePart type="family">Erdman</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">CS</namePart><namePart type="family">Goldsmith</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SR</namePart><namePart type="family">Zaki</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T</namePart><namePart type="family">Peret</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Emery</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Tong</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C</namePart><namePart type="family">Urbani</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JA</namePart><namePart type="family">Comer</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">W</namePart><namePart type="family">Lim</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">PE</namePart><namePart type="family">Rollin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SF</namePart><namePart type="family">Dowell</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AE</namePart><namePart type="family">Ling</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">CD</namePart><namePart type="family">Humphrey</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">WJ</namePart><namePart type="family">Shieh</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J</namePart><namePart type="family">Guarner</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">CD</namePart><namePart type="family">Paddock</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P</namePart><namePart type="family">Rota</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B</namePart><namePart type="family">Fields</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J</namePart><namePart type="family">DeRisi</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JY</namePart><namePart type="family">Yang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N</namePart><namePart type="family">Cox</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JM</namePart><namePart type="family">Hughes</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JW</namePart><namePart type="family">LeDuc</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">WJ</namePart><namePart type="family">Bellini</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">LJ</namePart><namePart type="family">Anderson</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S, Tong S, Urbani C, Comer JA, Lim W, Rollin PE, Dowell SF, Ling AE, Humphrey CD, Shieh WJ, Guarner J, Paddock CD, Rota P, Fields B, DeRisi J, Yang JY, Cox N, Hughes JM, LeDuc JW, Bellini WJ, Anderson LJ. 2003. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med 348: 1953–1966.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>348</number></detail><extent unit="pages"><start>1953</start><end>1966</end></extent></part><relatedItem type="host"><titleInfo><title>N Engl J Med</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>348</number></detail><extent unit="pages"><start>1953</start><end>1966</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit12"><titleInfo><title>Nucleic acid sequence‐based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses</title></titleInfo><name type="personal"><namePart type="given">RS</namePart><namePart type="family">Lanciotti</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AJ</namePart><namePart type="family">Kerst</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Lanciotti RS, Kerst AJ. 2001. Nucleic acid sequence‐based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses. J Clin Microbiol 39: 4506–4513.</note><part><date>2001</date><detail type="volume"><caption>vol.</caption><number>39</number></detail><extent unit="pages"><start>4506</start><end>4513</end></extent></part><relatedItem type="host"><titleInfo><title>J Clin Microbiol</title></titleInfo><part><date>2001</date><detail type="volume"><caption>vol.</caption><number>39</number></detail><extent unit="pages"><start>4506</start><end>4513</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit13"><titleInfo><title>Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme‐linked immunosorbent assay</title></titleInfo><name type="personal"><namePart type="given">SK</namePart><namePart type="family">Lau</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">PC</namePart><namePart type="family">Woo</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">BH</namePart><namePart type="family">Wong</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">HW</namePart><namePart type="family">Tsoi</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">GK</namePart><namePart type="family">Woo</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">RW</namePart><namePart type="family">Poon</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KH</namePart><namePart type="family">Chan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">WI</namePart><namePart type="family">Wei</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JS</namePart><namePart type="family">Peiris</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KY</namePart><namePart type="family">Yuen</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Lau SK, Woo PC, Wong BH, Tsoi HW, Woo GK, Poon RW, Chan KH, Wei WI, Peiris JS, Yuen KY. 2004. Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme‐linked immunosorbent assay. J Clin Microbiol 42: 2884–2889.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>42</number></detail><extent unit="pages"><start>2884</start><end>2889</end></extent></part><relatedItem type="host"><titleInfo><title>J Clin Microbiol</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>42</number></detail><extent unit="pages"><start>2884</start><end>2889</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit14"><titleInfo><title>Performance and Cost evaluation of one commercial and six in‐house conventional and real‐time reverse transcription‐PCR assays for detection of severe acute respiratory syndrome coronavirus</title></titleInfo><name type="personal"><namePart type="given">JB</namePart><namePart type="family">Mahony</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Petrich</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L</namePart><namePart type="family">Louie</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">X</namePart><namePart type="family">Song</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Chong</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Smieja</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Chernesky</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Loeb</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Richardson</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Mahony JB, Petrich A, Louie L, Song X, Chong S, Smieja M, Chernesky M, Loeb M, Richardson S. 2004. Performance and Cost evaluation of one commercial and six in‐house conventional and real‐time reverse transcription‐PCR assays for detection of severe acute respiratory syndrome coronavirus. J Clin Microbiol 42: 1471–1476.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>42</number></detail><extent unit="pages"><start>1471</start><end>1476</end></extent></part><relatedItem type="host"><titleInfo><title>J Clin Microbiol</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>42</number></detail><extent unit="pages"><start>1471</start><end>1476</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit15"><titleInfo><title>The Genome sequence of the SARS‐associated coronavirus</title></titleInfo><name type="personal"><namePart type="given">MA</namePart><namePart type="family">Marra</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SJ</namePart><namePart type="family">Jones</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">CR</namePart><namePart type="family">Astell</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">RA</namePart><namePart type="family">Holt</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Brooks‐Wilson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">YS</namePart><namePart type="family">Butterfield</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J</namePart><namePart type="family">Khattra</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JK</namePart><namePart type="family">Asano</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SA</namePart><namePart type="family">Barber</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SY</namePart><namePart type="family">Chan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Cloutier</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SM</namePart><namePart type="family">Coughlin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D</namePart><namePart type="family">Freeman</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N</namePart><namePart type="family">Girn</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">OL</namePart><namePart type="family">Griffith</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SR</namePart><namePart type="family">Leach</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Mayo</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H</namePart><namePart type="family">McDonald</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SB</namePart><namePart type="family">Montgomery</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">PK</namePart><namePart type="family">Pandoh</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AS</namePart><namePart type="family">Petrescu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AG</namePart><namePart type="family">Robertson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JE</namePart><namePart type="family">Schein</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Siddiqui</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">DE</namePart><namePart type="family">Smailus</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JM</namePart><namePart type="family">Stott</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">GS</namePart><namePart type="family">Yang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">F</namePart><namePart type="family">Plummer</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Andonov</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H</namePart><namePart type="family">Artsob</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N</namePart><namePart type="family">Bastien</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K</namePart><namePart type="family">Bernard</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">TF</namePart><namePart type="family">Booth</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D</namePart><namePart type="family">Bowness</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Czub</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Drebot</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L</namePart><namePart type="family">Fernando</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R</namePart><namePart type="family">Flick</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Garbutt</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Gray</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Grolla</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Jones</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H</namePart><namePart type="family">Feldmann</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Meyers</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Kabani</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y</namePart><namePart type="family">Li</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Normand</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">U</namePart><namePart type="family">Stroher</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">GA</namePart><namePart type="family">Tipples</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Tyler</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R</namePart><namePart type="family">Vogrig</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D</namePart><namePart type="family">Ward</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B</namePart><namePart type="family">Watson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">RC</namePart><namePart type="family">Brunham</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Krajden</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Petric</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">DM</namePart><namePart type="family">Skowronski</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C</namePart><namePart type="family">Upton</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">RL</namePart><namePart type="family">Roper</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Marra MA, Jones SJ, Astell CR, Holt RA, Brooks‐Wilson A, Butterfield YS, Khattra J, Asano JK, Barber SA, Chan SY, Cloutier A, Coughlin SM, Freeman D, Girn N, Griffith OL, Leach SR, Mayo M, McDonald H, Montgomery SB, Pandoh PK, Petrescu AS, Robertson AG, Schein JE, Siddiqui A, Smailus DE, Stott JM, Yang GS, Plummer F, Andonov A, Artsob H, Bastien N, Bernard K, Booth TF, Bowness D, Czub M, Drebot M, Fernando L, Flick R, Garbutt M, Gray M, Grolla A, Jones S, Feldmann H, Meyers A, Kabani A, Li Y, Normand S, Stroher U, Tipples GA, Tyler S, Vogrig R, Ward D, Watson B, Brunham RC, Krajden M, Petric M, Skowronski DM, Upton C, Roper RL. 2003. The Genome sequence of the SARS‐associated coronavirus. Science 300: 1399–1404.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>300</number></detail><extent unit="pages"><start>1399</start><end>1404</end></extent></part><relatedItem type="host"><titleInfo><title>Science</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>300</number></detail><extent unit="pages"><start>1399</start><end>1404</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit16"><titleInfo><title>Clinical progression and viral load in a community outbreak of coronavirus‐associated SARS pneumonia: A prospective study</title></titleInfo><name type="personal"><namePart type="given">JS</namePart><namePart type="family">Peiris</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">CM</namePart><namePart type="family">Chu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">VC</namePart><namePart type="family">Cheng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KS</namePart><namePart type="family">Chan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">IF</namePart><namePart type="family">Hung</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">LL</namePart><namePart type="family">Poon</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KI</namePart><namePart type="family">Law</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">BS</namePart><namePart type="family">Tang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">TY</namePart><namePart type="family">Hon</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">CS</namePart><namePart type="family">Chan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KH</namePart><namePart type="family">Chan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JS</namePart><namePart type="family">Ng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">BJ</namePart><namePart type="family">Zheng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">WL</namePart><namePart type="family">Ng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">RW</namePart><namePart type="family">Lai</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y</namePart><namePart type="family">Guan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KY</namePart><namePart type="family">Yuen</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Peiris JS, Chu CM, Cheng VC, Chan KS, Hung IF, Poon LL, Law KI, Tang BS, Hon TY, Chan CS, Chan KH, Ng JS, Zheng BJ, Ng WL, Lai RW, Guan Y, Yuen KY. 2003. Clinical progression and viral load in a community outbreak of coronavirus‐associated SARS pneumonia: A prospective study. Lancet 361: 1767–1772.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>361</number></detail><extent unit="pages"><start>1767</start><end>1772</end></extent></part><relatedItem type="host"><titleInfo><title>Lancet</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>361</number></detail><extent unit="pages"><start>1767</start><end>1772</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit17"><titleInfo><title>Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real‐time quantitative reverse transcription‐PCR assays</title></titleInfo><name type="personal"><namePart type="given">LL</namePart><namePart type="family">Poon</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KH</namePart><namePart type="family">Chan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">OK</namePart><namePart type="family">Wong</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">TK</namePart><namePart type="family">Cheung</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I</namePart><namePart type="family">Ng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B</namePart><namePart type="family">Zheng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">WH</namePart><namePart type="family">Seto</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KY</namePart><namePart type="family">Yuen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y</namePart><namePart type="family">Guan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JS</namePart><namePart type="family">Peiris</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Poon LL, Chan KH, Wong OK, Cheung TK, Ng I, Zheng B, Seto WH, Yuen KY, Guan Y, Peiris JS. 2004. Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real‐time quantitative reverse transcription‐PCR assays. Clin Chem 50: 67–72.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>50</number></detail><extent unit="pages"><start>67</start><end>72</end></extent></part><relatedItem type="host"><titleInfo><title>Clin Chem</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>50</number></detail><extent unit="pages"><start>67</start><end>72</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit18"><titleInfo><title>Identification of severe acute respiratory syndrome in Canada</title></titleInfo><name type="personal"><namePart type="given">SM</namePart><namePart type="family">Poutanen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">DE</namePart><namePart type="family">Low</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B</namePart><namePart type="family">Henry</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Finkelstein</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D</namePart><namePart type="family">Rose</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K</namePart><namePart type="family">Green</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R</namePart><namePart type="family">Tellier</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R</namePart><namePart type="family">Draker</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D</namePart><namePart type="family">Adachi</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Ayers</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AK</namePart><namePart type="family">Chan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">DM</namePart><namePart type="family">Skowronski</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">I</namePart><namePart type="family">Salit</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AE</namePart><namePart type="family">Simor</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AS</namePart><namePart type="family">Slutsky</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">PW</namePart><namePart type="family">Doyle</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Krajden</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Petric</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">RC</namePart><namePart type="family">Brunham</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AJ</namePart><namePart type="family">McGeer</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Poutanen SM, Low DE, Henry B, Finkelstein S, Rose D, Green K, Tellier R, Draker R, Adachi D, Ayers M, Chan AK, Skowronski DM, Salit I, Simor AE, Slutsky AS, Doyle PW, Krajden M, Petric M, Brunham RC, McGeer AJ. 2003. Identification of severe acute respiratory syndrome in Canada. N Engl J Med 348: 1995–2005.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>348</number></detail><extent unit="pages"><start>1995</start><end>2005</end></extent></part><relatedItem type="host"><titleInfo><title>N Engl J Med</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>348</number></detail><extent unit="pages"><start>1995</start><end>2005</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit19"><titleInfo><title>SARS associated coronavirus has a recombinant polymerase and coronaviruses have a history of host‐shifting</title></titleInfo><name type="personal"><namePart type="given">JS</namePart><namePart type="family">Rest</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">DP</namePart><namePart type="family">Mindell</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Rest JS, Mindell DP. 2003. SARS associated coronavirus has a recombinant polymerase and coronaviruses have a history of host‐shifting. Infect Genet Evol 3: 219–225.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>3</number></detail><extent unit="pages"><start>219</start><end>225</end></extent></part><relatedItem type="host"><titleInfo><title>Infect Genet Evol</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>3</number></detail><extent unit="pages"><start>219</start><end>225</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit20"><titleInfo><title>Characterization of a novel coronavirus associated with severe acute respiratory syndrome</title></titleInfo><name type="personal"><namePart type="given">PA</namePart><namePart type="family">Rota</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">MS</namePart><namePart type="family">Oberste</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SS</namePart><namePart type="family">Monroe</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">WA</namePart><namePart type="family">Nix</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R</namePart><namePart type="family">Campagnoli</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JP</namePart><namePart type="family">Icenogle</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Penaranda</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B</namePart><namePart type="family">Bankamp</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K</namePart><namePart type="family">Maher</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">MH</namePart><namePart type="family">Chen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Tong</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Tamin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L</namePart><namePart type="family">Lowe</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Frace</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JL</namePart><namePart type="family">DeRisi</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Q</namePart><namePart type="family">Chen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D</namePart><namePart type="family">Wang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">DD</namePart><namePart type="family">Erdman</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">TC</namePart><namePart type="family">Peret</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C</namePart><namePart type="family">Burns</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">TG</namePart><namePart type="family">Ksiazek</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">PE</namePart><namePart type="family">Rollin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">A</namePart><namePart type="family">Sanchez</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Liffick</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B</namePart><namePart type="family">Holloway</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">J</namePart><namePart type="family">Limor</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K</namePart><namePart type="family">McCaustland</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M</namePart><namePart type="family">Olsen‐Rasmussen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">R</namePart><namePart type="family">Fouchier</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">Gunther</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AD</namePart><namePart type="family">Osterhaus</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C</namePart><namePart type="family">Drosten</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">MA</namePart><namePart type="family">Pallansch</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">LJ</namePart><namePart type="family">Anderson</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">WJ</namePart><namePart type="family">Bellini</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Rota PA, Oberste MS, Monroe SS, Nix WA, Campagnoli R, Icenogle JP, Penaranda S, Bankamp B, Maher K, Chen MH, Tong S, Tamin A, Lowe L, Frace M, DeRisi JL, Chen Q, Wang D, Erdman DD, Peret TC, Burns C, Ksiazek TG, Rollin PE, Sanchez A, Liffick S, Holloway B, Limor J, McCaustland K, Olsen‐Rasmussen M, Fouchier R, Gunther S, Osterhaus AD, Drosten C, Pallansch MA, Anderson LJ, Bellini WJ. 2003. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 300: 1394–1399.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>300</number></detail><extent unit="pages"><start>1394</start><end>1399</end></extent></part><relatedItem type="host"><titleInfo><title>Science</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>300</number></detail><extent unit="pages"><start>1394</start><end>1399</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit21"><titleInfo><title>Comparative full‐length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection</title></titleInfo><name type="personal"><namePart type="given">YJ</namePart><namePart type="family">Ruan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">CL</namePart><namePart type="family">Wei</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">AL</namePart><namePart type="family">Ee</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">VB</namePart><namePart type="family">Vega</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H</namePart><namePart type="family">Thoreau</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">ST</namePart><namePart type="family">Su</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JM</namePart><namePart type="family">Chia</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P</namePart><namePart type="family">Ng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KP</namePart><namePart type="family">Chiu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L</namePart><namePart type="family">Lim</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T</namePart><namePart type="family">Zhang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">CK</namePart><namePart type="family">Peng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">EO</namePart><namePart type="family">Lin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">NM</namePart><namePart type="family">Lee</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SL</namePart><namePart type="family">Yee</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">LF</namePart><namePart type="family">Ng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">RE</namePart><namePart type="family">Chee</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">LW</namePart><namePart type="family">Stanton</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">PM</namePart><namePart type="family">Long</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">ET</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Ruan YJ, Wei CL, Ee AL, Vega VB, Thoreau H, Su ST, Chia JM, Ng P, Chiu KP, Lim L, Zhang T, Peng CK, Lin EO, Lee NM, Yee SL, Ng LF, Chee RE, Stanton LW, Long PM, Liu ET. 2003. Comparative full‐length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection. Lancet 361: 1779–1785.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>361</number></detail><extent unit="pages"><start>1779</start><end>1785</end></extent></part><relatedItem type="host"><titleInfo><title>Lancet</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>361</number></detail><extent unit="pages"><start>1779</start><end>1785</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit22"><titleInfo><title>A multicentre collaboration to investigate the cause of severe acute respiratory syndrome</title></titleInfo><name type="corporate"><namePart>World Health Organization Multicentre Collaborative Network for Severe Acute Respiratory Syndrome Diagnosis</namePart></name><genre>journal-article</genre><note type="citation/reference">World Health Organization Multicentre Collaborative Network for Severe Acute Respiratory Syndrome Diagnosis. 2003. A multicentre collaboration to investigate the cause of severe acute respiratory syndrome. Lancet 361: 1730–1733.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>361</number></detail><extent unit="pages"><start>1730</start><end>1733</end></extent></part><relatedItem type="host"><titleInfo><title>Lancet</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>361</number></detail><extent unit="pages"><start>1730</start><end>1733</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit23"><titleInfo><title>Serologic and Molecular Biologic Methods for SARS‐associated Coronavirus Infection, Taiwan</title></titleInfo><name type="personal"><namePart type="given">HS</namePart><namePart type="family">Wu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SC</namePart><namePart type="family">Chiu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">TC</namePart><namePart type="family">Tseng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SF</namePart><namePart type="family">Lin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JH</namePart><namePart type="family">Lin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">YH</namePart><namePart type="family">Hsu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">MC</namePart><namePart type="family">Wang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">TL</namePart><namePart type="family">Lin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">WZ</namePart><namePart type="family">Yang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">TL</namePart><namePart type="family">Ferng</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KH</namePart><namePart type="family">Huang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">LC</namePart><namePart type="family">Hsu</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">LL</namePart><namePart type="family">Lee</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JY</namePart><namePart type="family">Yang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">HY</namePart><namePart type="family">Chen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SP</namePart><namePart type="family">Su</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SY</namePart><namePart type="family">Yang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">SY</namePart><namePart type="family">Lin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">TH</namePart><namePart type="family">Lin</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">IS</namePart><namePart type="family">Su</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Wu HS, Chiu SC, Tseng TC, Lin SF, Lin JH, Hsu YH, Wang MC, Lin TL, Yang WZ, Ferng TL, Huang KH, Hsu LC, Lee LL, Yang JY, Chen HY, Su SP, Yang SY, Lin SY, Lin TH, Su IS. 2004. Serologic and Molecular Biologic Methods for SARS‐associated Coronavirus Infection, Taiwan. Emerg Infect Dis 10: 304–310.</note><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>10</number></detail><extent unit="pages"><start>304</start><end>310</end></extent></part><relatedItem type="host"><titleInfo><title>Emerg Infect Dis</title></titleInfo><part><date>2004</date><detail type="volume"><caption>vol.</caption><number>10</number></detail><extent unit="pages"><start>304</start><end>310</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit24"><titleInfo><title>Evaluation of reverse transcription‐PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus</title></titleInfo><name type="personal"><namePart type="given">WC</namePart><namePart type="family">Yam</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KH</namePart><namePart type="family">Chan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">LL</namePart><namePart type="family">Poon</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y</namePart><namePart type="family">Guan</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">KY</namePart><namePart type="family">Yuen</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">WH</namePart><namePart type="family">Seto</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">JS</namePart><namePart type="family">Peiris</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>journal-article</genre><note type="citation/reference">Yam WC, Chan KH, Poon LL, Guan Y, Yuen KY, Seto WH, Peiris JS. 2003. Evaluation of reverse transcription‐PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus. J Clin Microbiol 41: 4521–4524.</note><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>41</number></detail><extent unit="pages"><start>4521</start><end>4524</end></extent></part><relatedItem type="host"><titleInfo><title>J Clin Microbiol</title></titleInfo><part><date>2003</date><detail type="volume"><caption>vol.</caption><number>41</number></detail><extent unit="pages"><start>4521</start><end>4524</end></extent></part></relatedItem></relatedItem><relatedItem type="references" displayLabel="cit25"><titleInfo><title>Real time detection of SARS related coronavirus using nucleic acid sequence based amplification (NASBA) and molecular beacons</title></titleInfo><name type="personal"><namePart type="given">F</namePart><namePart type="family">Zhang</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">S</namePart><namePart type="family">van der Laar</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">D</namePart><namePart type="family">Zimmerman</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P</namePart><namePart type="family">Sillekens</namePart><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">CC</namePart><namePart type="family">Ginocchio</namePart><role><roleTerm type="text">author</roleTerm></role></name><genre>other</genre><part><date>2004</date></part></relatedItem><identifier type="istex">098DFCB2D53357E19C5E683F42475B33A4D27C1E</identifier><identifier type="ark">ark:/67375/WNG-ZD79ZC6B-F</identifier><identifier type="DOI">10.1002/jmv.20498</identifier><identifier type="ArticleID">JMV20498</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2005 Wiley‐Liss, Inc.</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-L0C46X92-X">wiley</recordContentSource><recordOrigin>Converted from (version ) to MODS version 3.6.</recordOrigin><recordCreationDate encoding="w3cdtf">2019-11-15</recordCreationDate></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/WNG-ZD79ZC6B-F/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000394 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000394 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:098DFCB2D53357E19C5E683F42475B33A4D27C1E
|texte= Real‐time NASBA detection of SARS‐associated coronavirus and comparison with real‐time reverse transcription‐PCR
}}
| This area was generated with Dilib version V0.6.33. |  |