Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein.
Identifieur interne : 004860 ( Main/Merge ); précédent : 004859; suivant : 004861Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein.
Auteurs : Jaehwan You [Royaume-Uni] ; Brian K. Dove [Royaume-Uni] ; Luis Enjuanes [Espagne] ; Marta L. Dediego [Espagne] ; Enrique Alvarez [Espagne] ; Gareth Howell [Royaume-Uni] ; Paul Heinen [Royaume-Uni] ; Maria Zambon [Royaume-Uni] ; Julian A. Hiscox [Royaume-Uni]Source :
- The Journal of general virology [ 0022-1317 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Biologie informatique, Cellules Vero, Cytoplasme (), Données de séquences moléculaires, Délétion de séquence, Microscopie confocale, Nucléole (), Protéines nucléocapside (), Protéines nucléocapside (analyse), Protéines nucléocapside (génétique), Signaux de localisation nucléaire, Similitude de séquences, Structure tertiaire des protéines, Séquence d'acides aminés, Virus du SRAS (génétique), Virus du SRAS (physiologie).
- MESH :
- analyse : Protéines nucléocapside.
- génétique : Protéines nucléocapside, Virus du SRAS.
- physiologie : Virus du SRAS.
- Animaux, Biologie informatique, Cellules Vero, Cytoplasme, Données de séquences moléculaires, Délétion de séquence, Microscopie confocale, Nucléole, Protéines nucléocapside, Signaux de localisation nucléaire, Similitude de séquences, Structure tertiaire des protéines, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Cell Nucleolus (chemistry), Chlorocebus aethiops, Computational Biology, Cytoplasm (chemistry), Microscopy, Confocal, Molecular Sequence Data, Nuclear Localization Signals, Nucleocapsid Proteins (analysis), Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Protein Structure, Tertiary, SARS Virus (genetics), SARS Virus (physiology), Sequence Deletion, Sequence Homology, Vero Cells.
- MESH :
- chemical , analysis : Nucleocapsid Proteins.
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , genetics : Nucleocapsid Proteins.
- chemical : Nuclear Localization Signals.
- chemistry : Cell Nucleolus, Cytoplasm.
- genetics : SARS Virus.
- physiology : SARS Virus.
- Amino Acid Sequence, Animals, Chlorocebus aethiops, Computational Biology, Microscopy, Confocal, Molecular Sequence Data, Protein Structure, Tertiary, Sequence Deletion, Sequence Homology, Vero Cells.
Abstract
The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein.
DOI: 10.1099/vir.0.81076-0
PubMed: 16298975
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002441
- to stream PubMed, to step Curation: 002441
- to stream PubMed, to step Checkpoint: 002455
- to stream Ncbi, to step Merge: 001271
- to stream Ncbi, to step Curation: 001271
- to stream Ncbi, to step Checkpoint: 001271
Links to Exploration step
pubmed:16298975Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein.</title>
<author><name sortKey="You, Jaehwan" sort="You, Jaehwan" uniqKey="You J" first="Jaehwan" last="You">Jaehwan You</name>
<affiliation wicri:level="4"><nlm:affiliation>Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds, Leeds LS2 9JT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds, Leeds LS2 9JT</wicri:regionArea>
<orgName type="university">Université de Leeds</orgName>
<placeName><settlement type="city">Leeds</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Yorkshire-et-Humber</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dove, Brian K" sort="Dove, Brian K" uniqKey="Dove B" first="Brian K" last="Dove">Brian K. Dove</name>
<affiliation wicri:level="4"><nlm:affiliation>Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds, Leeds LS2 9JT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds, Leeds LS2 9JT</wicri:regionArea>
<orgName type="university">Université de Leeds</orgName>
<placeName><settlement type="city">Leeds</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Yorkshire-et-Humber</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L" last="Dediego">Marta L. Dediego</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Alvarez, Enrique" sort="Alvarez, Enrique" uniqKey="Alvarez E" first="Enrique" last="Alvarez">Enrique Alvarez</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Howell, Gareth" sort="Howell, Gareth" uniqKey="Howell G" first="Gareth" last="Howell">Gareth Howell</name>
<affiliation wicri:level="4"><nlm:affiliation>Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT</wicri:regionArea>
<orgName type="university">Université de Leeds</orgName>
<placeName><settlement type="city">Leeds</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Yorkshire-et-Humber</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Heinen, Paul" sort="Heinen, Paul" uniqKey="Heinen P" first="Paul" last="Heinen">Paul Heinen</name>
<affiliation wicri:level="1"><nlm:affiliation>Health Protection Agency, London NW9 5HT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Health Protection Agency, London NW9 5HT</wicri:regionArea>
<wicri:noRegion>London NW9 5HT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name>
<affiliation wicri:level="1"><nlm:affiliation>Health Protection Agency, London NW9 5HT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Health Protection Agency, London NW9 5HT</wicri:regionArea>
<wicri:noRegion>London NW9 5HT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hiscox, Julian A" sort="Hiscox, Julian A" uniqKey="Hiscox J" first="Julian A" last="Hiscox">Julian A. Hiscox</name>
<affiliation wicri:level="4"><nlm:affiliation>Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT</wicri:regionArea>
<orgName type="university">Université de Leeds</orgName>
<placeName><settlement type="city">Leeds</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Yorkshire-et-Humber</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2005">2005</date>
<idno type="RBID">pubmed:16298975</idno>
<idno type="pmid">16298975</idno>
<idno type="doi">10.1099/vir.0.81076-0</idno>
<idno type="wicri:Area/PubMed/Corpus">002441</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002441</idno>
<idno type="wicri:Area/PubMed/Curation">002441</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002441</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002455</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002455</idno>
<idno type="wicri:Area/Ncbi/Merge">001271</idno>
<idno type="wicri:Area/Ncbi/Curation">001271</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001271</idno>
<idno type="wicri:doubleKey">0022-1317:2005:You J:subcellular:localization:of</idno>
<idno type="wicri:Area/Main/Merge">004860</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein.</title>
<author><name sortKey="You, Jaehwan" sort="You, Jaehwan" uniqKey="You J" first="Jaehwan" last="You">Jaehwan You</name>
<affiliation wicri:level="4"><nlm:affiliation>Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds, Leeds LS2 9JT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds, Leeds LS2 9JT</wicri:regionArea>
<orgName type="university">Université de Leeds</orgName>
<placeName><settlement type="city">Leeds</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Yorkshire-et-Humber</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dove, Brian K" sort="Dove, Brian K" uniqKey="Dove B" first="Brian K" last="Dove">Brian K. Dove</name>
<affiliation wicri:level="4"><nlm:affiliation>Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds, Leeds LS2 9JT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds, Leeds LS2 9JT</wicri:regionArea>
<orgName type="university">Université de Leeds</orgName>
<placeName><settlement type="city">Leeds</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Yorkshire-et-Humber</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L" last="Dediego">Marta L. Dediego</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Alvarez, Enrique" sort="Alvarez, Enrique" uniqKey="Alvarez E" first="Enrique" last="Alvarez">Enrique Alvarez</name>
<affiliation wicri:level="2"><nlm:affiliation>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid, Spain.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid</wicri:regionArea>
<placeName><region nuts="2" type="communauté">Communauté de Madrid</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Howell, Gareth" sort="Howell, Gareth" uniqKey="Howell G" first="Gareth" last="Howell">Gareth Howell</name>
<affiliation wicri:level="4"><nlm:affiliation>Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT</wicri:regionArea>
<orgName type="university">Université de Leeds</orgName>
<placeName><settlement type="city">Leeds</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Yorkshire-et-Humber</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Heinen, Paul" sort="Heinen, Paul" uniqKey="Heinen P" first="Paul" last="Heinen">Paul Heinen</name>
<affiliation wicri:level="1"><nlm:affiliation>Health Protection Agency, London NW9 5HT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Health Protection Agency, London NW9 5HT</wicri:regionArea>
<wicri:noRegion>London NW9 5HT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name>
<affiliation wicri:level="1"><nlm:affiliation>Health Protection Agency, London NW9 5HT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Health Protection Agency, London NW9 5HT</wicri:regionArea>
<wicri:noRegion>London NW9 5HT</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hiscox, Julian A" sort="Hiscox, Julian A" uniqKey="Hiscox J" first="Julian A" last="Hiscox">Julian A. Hiscox</name>
<affiliation wicri:level="4"><nlm:affiliation>Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.</nlm:affiliation>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea>Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT</wicri:regionArea>
<orgName type="university">Université de Leeds</orgName>
<placeName><settlement type="city">Leeds</settlement>
<region type="country">Angleterre</region>
<region type="région" nuts="1">Yorkshire-et-Humber</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">The Journal of general virology</title>
<idno type="ISSN">0022-1317</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Cell Nucleolus (chemistry)</term>
<term>Chlorocebus aethiops</term>
<term>Computational Biology</term>
<term>Cytoplasm (chemistry)</term>
<term>Microscopy, Confocal</term>
<term>Molecular Sequence Data</term>
<term>Nuclear Localization Signals</term>
<term>Nucleocapsid Proteins (analysis)</term>
<term>Nucleocapsid Proteins (chemistry)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Protein Structure, Tertiary</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (physiology)</term>
<term>Sequence Deletion</term>
<term>Sequence Homology</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Biologie informatique</term>
<term>Cellules Vero</term>
<term>Cytoplasme ()</term>
<term>Données de séquences moléculaires</term>
<term>Délétion de séquence</term>
<term>Microscopie confocale</term>
<term>Nucléole ()</term>
<term>Protéines nucléocapside ()</term>
<term>Protéines nucléocapside (analyse)</term>
<term>Protéines nucléocapside (génétique)</term>
<term>Signaux de localisation nucléaire</term>
<term>Similitude de séquences</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Nuclear Localization Signals</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Cell Nucleolus</term>
<term>Cytoplasm</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines nucléocapside</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Computational Biology</term>
<term>Microscopy, Confocal</term>
<term>Molecular Sequence Data</term>
<term>Protein Structure, Tertiary</term>
<term>Sequence Deletion</term>
<term>Sequence Homology</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Biologie informatique</term>
<term>Cellules Vero</term>
<term>Cytoplasme</term>
<term>Données de séquences moléculaires</term>
<term>Délétion de séquence</term>
<term>Microscopie confocale</term>
<term>Nucléole</term>
<term>Protéines nucléocapside</term>
<term>Signaux de localisation nucléaire</term>
<term>Similitude de séquences</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004860 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 004860 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Merge |type= RBID |clé= pubmed:16298975 |texte= Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:16298975" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
This area was generated with Dilib version V0.6.33. |