Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein.
Identifieur interne : 001271 ( Ncbi/Merge ); précédent : 001270; suivant : 001272Subcellular localization of the severe acute respiratory syndrome coronavirus nucleocapsid protein.
Auteurs : Jaehwan You [Royaume-Uni] ; Brian K. Dove [Royaume-Uni] ; Luis Enjuanes [Espagne] ; Marta L. Dediego [Espagne] ; Enrique Alvarez [Espagne] ; Gareth Howell [Royaume-Uni] ; Paul Heinen [Royaume-Uni] ; Maria Zambon [Royaume-Uni] ; Julian A. Hiscox [Royaume-Uni]Source :
- The Journal of general virology [ 0022-1317 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Biologie informatique, Cellules Vero, Cytoplasme (), Données de séquences moléculaires, Délétion de séquence, Microscopie confocale, Nucléole (), Protéines nucléocapside (), Protéines nucléocapside (analyse), Protéines nucléocapside (génétique), Signaux de localisation nucléaire, Similitude de séquences, Structure tertiaire des protéines, Séquence d'acides aminés, Virus du SRAS (génétique), Virus du SRAS (physiologie).
- MESH :
- analyse : Protéines nucléocapside.
- génétique : Protéines nucléocapside, Virus du SRAS.
- physiologie : Virus du SRAS.
- Animaux, Biologie informatique, Cellules Vero, Cytoplasme, Données de séquences moléculaires, Délétion de séquence, Microscopie confocale, Nucléole, Protéines nucléocapside, Signaux de localisation nucléaire, Similitude de séquences, Structure tertiaire des protéines, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Cell Nucleolus (chemistry), Chlorocebus aethiops, Computational Biology, Cytoplasm (chemistry), Microscopy, Confocal, Molecular Sequence Data, Nuclear Localization Signals, Nucleocapsid Proteins (analysis), Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Protein Structure, Tertiary, SARS Virus (genetics), SARS Virus (physiology), Sequence Deletion, Sequence Homology, Vero Cells.
- MESH :
- chemical , analysis : Nucleocapsid Proteins.
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , genetics : Nucleocapsid Proteins.
- chemical : Nuclear Localization Signals.
- chemistry : Cell Nucleolus, Cytoplasm.
- genetics : SARS Virus.
- physiology : SARS Virus.
- Amino Acid Sequence, Animals, Chlorocebus aethiops, Computational Biology, Microscopy, Confocal, Molecular Sequence Data, Protein Structure, Tertiary, Sequence Deletion, Sequence Homology, Vero Cells.
Abstract
The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein.
DOI: 10.1099/vir.0.81076-0
PubMed: 16298975
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pubmed:16298975Le document en format XML
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<term>Séquence d'acides aminés</term>
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<front><div type="abstract" xml:lang="en">The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein.</div>
</front>
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<Abstract><AbstractText>The coronavirus nucleocapsid (N) protein is a viral RNA-binding protein with multiple functions in terms of virus replication and modulating cell signalling pathways. N protein is composed of three distinct regions containing RNA-binding motif(s), and appropriate signals for modulating cell signalling. The subcellular localization of severe acute respiratory syndrome coronavirus (SARS-CoV) N protein was studied. In infected cells, SARS-CoV N protein localized exclusively to the cytoplasm. In contrast to the avian coronavirus N protein, overexpressed SARS-CoV N protein remained principally localized to the cytoplasm, with very few cells exhibiting nucleolar localization. Bioinformatic analysis and deletion mutagenesis coupled to confocal microscopy and live-cell imaging, revealed that SARS-CoV N protein regions I and III contained nuclear localization signals and region II contained a nucleolar retention signal. However, cytoplasmic localization was directed by region III and was the dominant localization signal in the protein.</AbstractText>
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<AffiliationInfo><Affiliation>Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB, CSIC), Campus Univ. Autonoma, 3 Darwin Street, Cantoblanco, 28049 Madrid, Spain.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Howell</LastName>
<ForeName>Gareth</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Heinen</LastName>
<ForeName>Paul</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>Health Protection Agency, London NW9 5HT, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zambon</LastName>
<ForeName>Maria</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Health Protection Agency, London NW9 5HT, UK.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hiscox</LastName>
<ForeName>Julian A</ForeName>
<Initials>JA</Initials>
<AffiliationInfo><Affiliation>Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds LS2 9JT, UK.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Institute of Molecular and Cellular Biology, Faculty of Biological Sciences, Garstang Building, University of Leeds, Leeds LS2 9JT, UK.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><Agency>Wellcome Trust</Agency>
<Country>United Kingdom</Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>J Gen Virol</MedlineTA>
<NlmUniqueID>0077340</NlmUniqueID>
<ISSNLinking>0022-1317</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019913">Nuclear Localization Signals</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C099602">nucleocapsid protein, Coronavirus</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002466" MajorTopicYN="N">Cell Nucleolus</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019295" MajorTopicYN="N">Computational Biology</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003593" MajorTopicYN="N">Cytoplasm</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018613" MajorTopicYN="N">Microscopy, Confocal</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019913" MajorTopicYN="N">Nuclear Localization Signals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017434" MajorTopicYN="N">Protein Structure, Tertiary</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017384" MajorTopicYN="N">Sequence Deletion</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017385" MajorTopicYN="N">Sequence Homology</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>11</Month>
<Day>22</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2005</Year>
<Month>12</Month>
<Day>21</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2005</Year>
<Month>11</Month>
<Day>22</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16298975</ArticleId>
<ArticleId IdType="doi">10.1099/vir.0.81076-0</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Espagne</li>
<li>Royaume-Uni</li>
</country>
<region><li>Angleterre</li>
<li>Communauté de Madrid</li>
<li>Yorkshire-et-Humber</li>
</region>
<settlement><li>Leeds</li>
</settlement>
<orgName><li>Université de Leeds</li>
</orgName>
</list>
<tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="You, Jaehwan" sort="You, Jaehwan" uniqKey="You J" first="Jaehwan" last="You">Jaehwan You</name>
</region>
<name sortKey="Dove, Brian K" sort="Dove, Brian K" uniqKey="Dove B" first="Brian K" last="Dove">Brian K. Dove</name>
<name sortKey="Heinen, Paul" sort="Heinen, Paul" uniqKey="Heinen P" first="Paul" last="Heinen">Paul Heinen</name>
<name sortKey="Hiscox, Julian A" sort="Hiscox, Julian A" uniqKey="Hiscox J" first="Julian A" last="Hiscox">Julian A. Hiscox</name>
<name sortKey="Howell, Gareth" sort="Howell, Gareth" uniqKey="Howell G" first="Gareth" last="Howell">Gareth Howell</name>
<name sortKey="Zambon, Maria" sort="Zambon, Maria" uniqKey="Zambon M" first="Maria" last="Zambon">Maria Zambon</name>
</country>
<country name="Espagne"><region name="Communauté de Madrid"><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name>
</region>
<name sortKey="Alvarez, Enrique" sort="Alvarez, Enrique" uniqKey="Alvarez E" first="Enrique" last="Alvarez">Enrique Alvarez</name>
<name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L" last="Dediego">Marta L. Dediego</name>
</country>
</tree>
</affiliations>
</record>
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