Surface ultrastructure of SARS coronavirus revealed by atomic force microscopy.
Identifieur interne : 004857 ( Main/Merge ); précédent : 004856; suivant : 004858Surface ultrastructure of SARS coronavirus revealed by atomic force microscopy.
Auteurs : Shiming Lin [Taïwan] ; Chih-Kung Lee ; Shih-Yuan Lee ; Chuan-Liang Kao ; Chii-Wann Lin ; An-Bang Wang ; Su-Ming Hsu ; Long-Sun HuangSource :
- Cellular microbiology [ 1462-5814 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , ultrastructure : Hemagglutinins, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- chemistry : SARS Virus.
- ultrastructure : SARS Virus, Virion.
- Microscopy, Atomic Force, Spike Glycoprotein, Coronavirus.
Abstract
Atomic force microscopy has been used to probe the surface nanostructures of severe acute respiratory syndrome coronavirus (SARS-CoV). Single crown-like virion was directly visualized and quantitative measurements of the dimensions for the structural proteins were provided. A corona of large, distinctive spikes in the envelope was measured after treatment with hydroxyoctanoic acid. High-resolution images revealed that the surface of each single SARS-CoV was surrounded with at least 15 spherical spikes having a diameter of 7.29 +/- 0.73 nm, which is in close agreement with that of S glycoproteins earlier predicted through the genomes of SARS-CoV. This study represents the first direct characterization of the surface ultrastructures of SARS-CoV particles at the nanometre scale and offers new prospects for mapping viral surface properties.
DOI: 10.1111/j.1462-5822.2005.00593.x
PubMed: 16309462
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002431
- to stream PubMed, to step Curation: 002431
- to stream PubMed, to step Checkpoint: 002453
- to stream Ncbi, to step Merge: 001281
- to stream Ncbi, to step Curation: 001281
- to stream Ncbi, to step Checkpoint: 001281
Links to Exploration step
pubmed:16309462Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Surface ultrastructure of SARS coronavirus revealed by atomic force microscopy.</title><author><name sortKey="Lin, Shiming" sort="Lin, Shiming" uniqKey="Lin S" first="Shiming" last="Lin">Shiming Lin</name><affiliation wicri:level="1"><nlm:affiliation>Center for Optoelectronic Biomedicine, National Taiwan University, Taipei, Taiwan. sml@ntumc.org</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Center for Optoelectronic Biomedicine, National Taiwan University, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Chih Kung" sort="Lee, Chih Kung" uniqKey="Lee C" first="Chih-Kung" last="Lee">Chih-Kung Lee</name></author><author><name sortKey="Lee, Shih Yuan" sort="Lee, Shih Yuan" uniqKey="Lee S" first="Shih-Yuan" last="Lee">Shih-Yuan Lee</name></author><author><name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name></author><author><name sortKey="Lin, Chii Wann" sort="Lin, Chii Wann" uniqKey="Lin C" first="Chii-Wann" last="Lin">Chii-Wann Lin</name></author><author><name sortKey="Wang, An Bang" sort="Wang, An Bang" uniqKey="Wang A" first="An-Bang" last="Wang">An-Bang Wang</name></author><author><name sortKey="Hsu, Su Ming" sort="Hsu, Su Ming" uniqKey="Hsu S" first="Su-Ming" last="Hsu">Su-Ming Hsu</name></author><author><name sortKey="Huang, Long Sun" sort="Huang, Long Sun" uniqKey="Huang L" first="Long-Sun" last="Huang">Long-Sun Huang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16309462</idno><idno type="pmid">16309462</idno><idno type="doi">10.1111/j.1462-5822.2005.00593.x</idno><idno type="wicri:Area/PubMed/Corpus">002431</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002431</idno><idno type="wicri:Area/PubMed/Curation">002431</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002431</idno><idno type="wicri:Area/PubMed/Checkpoint">002453</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002453</idno><idno type="wicri:Area/Ncbi/Merge">001281</idno><idno type="wicri:Area/Ncbi/Curation">001281</idno><idno type="wicri:Area/Ncbi/Checkpoint">001281</idno><idno type="wicri:doubleKey">1462-5814:2005:Lin S:surface:ultrastructure:of</idno><idno type="wicri:Area/Main/Merge">004857</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Surface ultrastructure of SARS coronavirus revealed by atomic force microscopy.</title><author><name sortKey="Lin, Shiming" sort="Lin, Shiming" uniqKey="Lin S" first="Shiming" last="Lin">Shiming Lin</name><affiliation wicri:level="1"><nlm:affiliation>Center for Optoelectronic Biomedicine, National Taiwan University, Taipei, Taiwan. sml@ntumc.org</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Center for Optoelectronic Biomedicine, National Taiwan University, Taipei</wicri:regionArea><wicri:noRegion>Taipei</wicri:noRegion></affiliation></author><author><name sortKey="Lee, Chih Kung" sort="Lee, Chih Kung" uniqKey="Lee C" first="Chih-Kung" last="Lee">Chih-Kung Lee</name></author><author><name sortKey="Lee, Shih Yuan" sort="Lee, Shih Yuan" uniqKey="Lee S" first="Shih-Yuan" last="Lee">Shih-Yuan Lee</name></author><author><name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name></author><author><name sortKey="Lin, Chii Wann" sort="Lin, Chii Wann" uniqKey="Lin C" first="Chii-Wann" last="Lin">Chii-Wann Lin</name></author><author><name sortKey="Wang, An Bang" sort="Wang, An Bang" uniqKey="Wang A" first="An-Bang" last="Wang">An-Bang Wang</name></author><author><name sortKey="Hsu, Su Ming" sort="Hsu, Su Ming" uniqKey="Hsu S" first="Su-Ming" last="Hsu">Su-Ming Hsu</name></author><author><name sortKey="Huang, Long Sun" sort="Huang, Long Sun" uniqKey="Huang L" first="Long-Sun" last="Huang">Long-Sun Huang</name></author></analytic><series><title level="j">Cellular microbiology</title><idno type="ISSN">1462-5814</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Hemagglutinins, Viral (ultrastructure)</term><term>Membrane Glycoproteins (ultrastructure)</term><term>Microscopy, Atomic Force</term><term>SARS Virus (chemistry)</term><term>SARS Virus (ultrastructure)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (ultrastructure)</term><term>Virion (ultrastructure)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (ultrastructure)</term><term>Hémagglutinines virales (ultrastructure)</term><term>Microscopie à force atomique</term><term>Protéines de l'enveloppe virale (ultrastructure)</term><term>Virion (ultrastructure)</term><term>Virus du SRAS ()</term><term>Virus du SRAS (ultrastructure)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="ultrastructure" xml:lang="en"><term>Hemagglutinins, Viral</term><term>Membrane Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>SARS Virus</term><term>Virion</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Microscopy, Atomic Force</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Hémagglutinines virales</term><term>Microscopie à force atomique</term><term>Protéines de l'enveloppe virale</term><term>Virion</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Atomic force microscopy has been used to probe the surface nanostructures of severe acute respiratory syndrome coronavirus (SARS-CoV). Single crown-like virion was directly visualized and quantitative measurements of the dimensions for the structural proteins were provided. A corona of large, distinctive spikes in the envelope was measured after treatment with hydroxyoctanoic acid. High-resolution images revealed that the surface of each single SARS-CoV was surrounded with at least 15 spherical spikes having a diameter of 7.29 +/- 0.73 nm, which is in close agreement with that of S glycoproteins earlier predicted through the genomes of SARS-CoV. This study represents the first direct characterization of the surface ultrastructures of SARS-CoV particles at the nanometre scale and offers new prospects for mapping viral surface properties.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004857 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 004857 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Merge
|type= RBID
|clé= pubmed:16309462
|texte= Surface ultrastructure of SARS coronavirus revealed by atomic force microscopy.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:16309462" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |