Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus.
Identifieur interne : 004806 ( Main/Merge ); précédent : 004805; suivant : 004807Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus.
Auteurs : Maofeng Qiu [République populaire de Chine] ; Jin Wang ; Hongxia Wang ; Zeliang Chen ; Erhei Dai ; Zhaobiao Guo ; Xiaoyi Wang ; Xin Pang ; Baoxing Fan ; Jie Wen ; Jian Wang ; Ruifu YangSource :
- Clinical and diagnostic laboratory immunology [ 1071-412X ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Anticorps antiviraux, Nucléocapside.
- diagnostic : Syndrome de détresse respiratoire de l'adulte.
- isolement et purification : Coronavirus.
- normes : Test ELISA.
- Coronavirus, Humains, Sensibilité et spécificité, Sites de fixation, Test ELISA, Tests sérologiques.
English descriptors
- KwdEn :
- Antibodies, Viral (analysis), Binding Sites, Coronavirus (chemistry), Coronavirus (isolation & purification), Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (standards), Humans, Nucleocapsid (analysis), Respiratory Distress Syndrome, Adult (diagnosis), Sensitivity and Specificity, Serologic Tests (methods).
- MESH :
- chemical , analysis : Antibodies, Viral.
- analysis : Nucleocapsid.
- chemistry : Coronavirus.
- diagnosis : Respiratory Distress Syndrome, Adult.
- isolation & purification : Coronavirus.
- methods : Enzyme-Linked Immunosorbent Assay, Serologic Tests.
- standards : Enzyme-Linked Immunosorbent Assay.
- Binding Sites, Humans, Sensitivity and Specificity.
Abstract
Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.
DOI: 10.1128/CDLI.12.3.474-476.2005
PubMed: 15753261
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- to stream PubMed, to step Checkpoint: 002404
- to stream Ncbi, to step Merge: 000E18
- to stream Ncbi, to step Curation: 000E18
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Links to Exploration step
pubmed:15753261Le document en format XML
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<term>Coronavirus (isolation & purification)</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Enzyme-Linked Immunosorbent Assay (standards)</term>
<term>Humans</term>
<term>Nucleocapsid (analysis)</term>
<term>Respiratory Distress Syndrome, Adult (diagnosis)</term>
<term>Sensitivity and Specificity</term>
<term>Serologic Tests (methods)</term>
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<term>Coronavirus ()</term>
<term>Coronavirus (isolement et purification)</term>
<term>Humains</term>
<term>Nucléocapside (analyse)</term>
<term>Sensibilité et spécificité</term>
<term>Sites de fixation</term>
<term>Syndrome de détresse respiratoire de l'adulte (diagnostic)</term>
<term>Test ELISA ()</term>
<term>Test ELISA (normes)</term>
<term>Tests sérologiques ()</term>
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<term>Nucléocapside</term>
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<front><div type="abstract" xml:lang="en">Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.</div>
</front>
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