Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus.
Identifieur interne : 002856 ( PubMed/Curation ); précédent : 002855; suivant : 002857Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus.
Auteurs : Maofeng Qiu [République populaire de Chine] ; Jin Wang ; Hongxia Wang ; Zeliang Chen ; Erhei Dai ; Zhaobiao Guo ; Xiaoyi Wang ; Xin Pang ; Baoxing Fan ; Jie Wen ; Jian Wang ; Ruifu YangSource :
- Clinical and diagnostic laboratory immunology [ 1071-412X ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Anticorps antiviraux, Nucléocapside.
- diagnostic : Syndrome de détresse respiratoire de l'adulte.
- isolement et purification : Coronavirus.
- normes : Test ELISA.
- Coronavirus, Humains, Sensibilité et spécificité, Sites de fixation, Test ELISA, Tests sérologiques.
English descriptors
- KwdEn :
- Antibodies, Viral (analysis), Binding Sites, Coronavirus (chemistry), Coronavirus (isolation & purification), Enzyme-Linked Immunosorbent Assay (methods), Enzyme-Linked Immunosorbent Assay (standards), Humans, Nucleocapsid (analysis), Respiratory Distress Syndrome, Adult (diagnosis), Sensitivity and Specificity, Serologic Tests (methods).
- MESH :
- chemical , analysis : Antibodies, Viral.
- analysis : Nucleocapsid.
- chemistry : Coronavirus.
- diagnosis : Respiratory Distress Syndrome, Adult.
- isolation & purification : Coronavirus.
- methods : Enzyme-Linked Immunosorbent Assay, Serologic Tests.
- standards : Enzyme-Linked Immunosorbent Assay.
- Binding Sites, Humans, Sensitivity and Specificity.
Abstract
Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.
DOI: 10.1128/CDLI.12.3.474-476.2005
PubMed: 15753261
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syndrome coronavirus.</title><author><name sortKey="Qiu, Maofeng" sort="Qiu, Maofeng" uniqKey="Qiu M" first="Maofeng" last="Qiu">Maofeng Qiu</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, No. 20, Dongdajie, Fengtai District, Beijing 100071</wicri:regionArea></affiliation></author><author><name sortKey="Wang, Jin" sort="Wang, Jin" uniqKey="Wang J" first="Jin" last="Wang">Jin Wang</name></author><author><name sortKey="Wang, Hongxia" sort="Wang, Hongxia" uniqKey="Wang H" first="Hongxia" last="Wang">Hongxia Wang</name></author><author><name sortKey="Chen, Zeliang" sort="Chen, Zeliang" uniqKey="Chen Z" first="Zeliang" last="Chen">Zeliang Chen</name></author><author><name sortKey="Dai, Erhei" sort="Dai, Erhei" uniqKey="Dai E" first="Erhei" last="Dai">Erhei Dai</name></author><author><name sortKey="Guo, Zhaobiao" sort="Guo, Zhaobiao" uniqKey="Guo Z" first="Zhaobiao" last="Guo">Zhaobiao Guo</name></author><author><name sortKey="Wang, Xiaoyi" sort="Wang, Xiaoyi" uniqKey="Wang X" first="Xiaoyi" last="Wang">Xiaoyi Wang</name></author><author><name sortKey="Pang, Xin" sort="Pang, Xin" uniqKey="Pang X" first="Xin" last="Pang">Xin Pang</name></author><author><name sortKey="Fan, Baoxing" sort="Fan, Baoxing" uniqKey="Fan B" first="Baoxing" last="Fan">Baoxing Fan</name></author><author><name sortKey="Wen, Jie" sort="Wen, Jie" uniqKey="Wen J" first="Jie" last="Wen">Jie Wen</name></author><author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name></author><author><name sortKey="Yang, Ruifu" sort="Yang, Ruifu" uniqKey="Yang R" first="Ruifu" last="Yang">Ruifu Yang</name></author></analytic><series><title level="j">Clinical and diagnostic laboratory immunology</title><idno type="ISSN">1071-412X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (analysis)</term><term>Binding Sites</term><term>Coronavirus (chemistry)</term><term>Coronavirus (isolation & purification)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Enzyme-Linked Immunosorbent Assay (standards)</term><term>Humans</term><term>Nucleocapsid (analysis)</term><term>Respiratory Distress Syndrome, Adult (diagnosis)</term><term>Sensitivity and Specificity</term><term>Serologic Tests (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Anticorps antiviraux (analyse)</term><term>Coronavirus ()</term><term>Coronavirus (isolement et purification)</term><term>Humains</term><term>Nucléocapside 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portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15753261</PMID><DateCompleted><Year>2005</Year><Month>08</Month><Day>19</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1071-412X</ISSN><JournalIssue CitedMedium="Print"><Volume>12</Volume><Issue>3</Issue><PubDate><Year>2005</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Clinical and diagnostic laboratory immunology</Title><ISOAbbreviation>Clin. 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Immunol.</ISOAbbreviation></Journal><ArticleTitle>Use of the COOH portion of the nucleocapsid protein in an antigen-capturing enzyme-linked immunosorbent assay for specific and sensitive detection of severe acute respiratory syndrome coronavirus.</ArticleTitle><Pagination><MedlinePgn>474-6</MedlinePgn></Pagination><Abstract><AbstractText>Antibody detection with a recombinant COOH portion of the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein, N13 (amino acids 221 to 422), was demonstrated to be more specific and sensitive than that with the full-length N protein, and an N13-based antigen-capturing enzyme-linked immunosorbent assay providing a convenient and specific test for serodiagnosis and epidemiological study of SARS was developed.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Qiu</LastName><ForeName>Maofeng</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute of Microbiology and Epidemiology, 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