Amino terminus of the SARS coronavirus protein 3a elicits strong, potentially protective humoral responses in infected patients
Identifieur interne : 004717 ( Main/Merge ); précédent : 004716; suivant : 004718Amino terminus of the SARS coronavirus protein 3a elicits strong, potentially protective humoral responses in infected patients
Auteurs : XIAOFEN ZHONG [Hong Kong] ; ZUFENG GUO [Hong Kong] ; HUANGHAO YANG [Hong Kong] ; LISHENG PENG [Hong Kong] ; YONG XIE [Hong Kong] ; Tin-Yau Wong [Hong Kong] ; Sik-To Lai [Hong Kong] ; ZHIHONG GUO [Hong Kong]Source :
- Journal of general virology [ 0022-1317 ] ; 2006.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Homme.
English descriptors
- KwdEn :
Abstract
The 3a protein of severe acute respiratory syndrome (SARS)-associated coronavirus is expressed and transported to the plasma membrane in tissue cells of infected patients. Its short N-terminal ectodomain was found to elicit strong humoral responses in half of the patients who had recovered from SARS. The ectodomain-specific antibodies from the convalescent-phase plasma readily recognized and induced destruction of 3a-expressing cells in the presence of the human complement system, demonstrating their potential ability to provide immune protection by recognizing and eliminating SARS coronavirus-infected cells that express the target protein. In addition, when coupled to a carrier protein, the ectodomain peptide elicited 3a-specific antibodies in mice and rabbit at high titres. These results showed that the N terminus of the 3a protein is highly immunogenic and elicits potentially protective humoral responses in infected patients. Therefore, the short extracellular domain may be a valuable immunogen in the development of a vaccine for infectious SARS.
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000552
- to stream PascalFrancis, to step Curation: 000438
- to stream PascalFrancis, to step Checkpoint: 000516
Links to Exploration step
Pascal:06-0099007Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Amino terminus of the SARS coronavirus protein 3a elicits strong, potentially protective humoral responses in infected patients</title>
<author><name sortKey="Xiaofen Zhong" sort="Xiaofen Zhong" uniqKey="Xiaofen Zhong" last="Xiaofen Zhong">XIAOFEN ZHONG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zufeng Guo" sort="Zufeng Guo" uniqKey="Zufeng Guo" last="Zufeng Guo">ZUFENG GUO</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Biotechnology Research Institute, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Huanghao Yang" sort="Huanghao Yang" uniqKey="Huanghao Yang" last="Huanghao Yang">HUANGHAO YANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Biotechnology Research Institute, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lisheng Peng" sort="Lisheng Peng" uniqKey="Lisheng Peng" last="Lisheng Peng">LISHENG PENG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yong Xie" sort="Yong Xie" uniqKey="Yong Xie" last="Yong Xie">YONG XIE</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biology, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wong, Tin Yau" sort="Wong, Tin Yau" uniqKey="Wong T" first="Tin-Yau" last="Wong">Tin-Yau Wong</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Princess Margaret Hospital</s1>
<s3>HKG</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Princess Margaret Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lai, Sik To" sort="Lai, Sik To" uniqKey="Lai S" first="Sik-To" last="Lai">Sik-To Lai</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Princess Margaret Hospital</s1>
<s3>HKG</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Princess Margaret Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhihong Guo" sort="Zhihong Guo" uniqKey="Zhihong Guo" last="Zhihong Guo">ZHIHONG GUO</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Biotechnology Research Institute, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">06-0099007</idno>
<date when="2006">2006</date>
<idno type="stanalyst">PASCAL 06-0099007 INIST</idno>
<idno type="RBID">Pascal:06-0099007</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000552</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000438</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000516</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000516</idno>
<idno type="wicri:doubleKey">0022-1317:2006:Xiaofen Zhong:amino:terminus:of</idno>
<idno type="wicri:Area/Main/Merge">004717</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Amino terminus of the SARS coronavirus protein 3a elicits strong, potentially protective humoral responses in infected patients</title>
<author><name sortKey="Xiaofen Zhong" sort="Xiaofen Zhong" uniqKey="Xiaofen Zhong" last="Xiaofen Zhong">XIAOFEN ZHONG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zufeng Guo" sort="Zufeng Guo" uniqKey="Zufeng Guo" last="Zufeng Guo">ZUFENG GUO</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Biotechnology Research Institute, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Huanghao Yang" sort="Huanghao Yang" uniqKey="Huanghao Yang" last="Huanghao Yang">HUANGHAO YANG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Biotechnology Research Institute, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lisheng Peng" sort="Lisheng Peng" uniqKey="Lisheng Peng" last="Lisheng Peng">LISHENG PENG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yong Xie" sort="Yong Xie" uniqKey="Yong Xie" last="Yong Xie">YONG XIE</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biology, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>5 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wong, Tin Yau" sort="Wong, Tin Yau" uniqKey="Wong T" first="Tin-Yau" last="Wong">Tin-Yau Wong</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Princess Margaret Hospital</s1>
<s3>HKG</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Princess Margaret Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lai, Sik To" sort="Lai, Sik To" uniqKey="Lai S" first="Sik-To" last="Lai">Sik-To Lai</name>
<affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Princess Margaret Hospital</s1>
<s3>HKG</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Princess Margaret Hospital</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhihong Guo" sort="Zhihong Guo" uniqKey="Zhihong Guo" last="Zhihong Guo">ZHIHONG GUO</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Biotechnology Research Institute, The Hong Kong University of Science and Technology</s1>
<s2>Clear Water Bay, Kowloon</s2>
<s3>HKG</s3>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Hong Kong</country>
<wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Journal of general virology</title>
<title level="j" type="abbreviated">J. gen. virol.</title>
<idno type="ISSN">0022-1317</idno>
<imprint><date when="2006">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Journal of general virology</title>
<title level="j" type="abbreviated">J. gen. virol.</title>
<idno type="ISSN">0022-1317</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Human</term>
<term>Humoral immunity</term>
<term>Infection</term>
<term>Microbiology</term>
<term>Protein</term>
<term>Severe acute respiratory syndrome virus</term>
<term>Virology</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term>
<term>Homme</term>
<term>Protéine</term>
<term>Immunité humorale</term>
<term>Microbiologie</term>
<term>Virologie</term>
<term>Infection</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The 3a protein of severe acute respiratory syndrome (SARS)-associated coronavirus is expressed and transported to the plasma membrane in tissue cells of infected patients. Its short N-terminal ectodomain was found to elicit strong humoral responses in half of the patients who had recovered from SARS. The ectodomain-specific antibodies from the convalescent-phase plasma readily recognized and induced destruction of 3a-expressing cells in the presence of the human complement system, demonstrating their potential ability to provide immune protection by recognizing and eliminating SARS coronavirus-infected cells that express the target protein. In addition, when coupled to a carrier protein, the ectodomain peptide elicited 3a-specific antibodies in mice and rabbit at high titres. These results showed that the N terminus of the 3a protein is highly immunogenic and elicits potentially protective humoral responses in infected patients. Therefore, the short extracellular domain may be a valuable immunogen in the development of a vaccine for infectious SARS.</div>
</front>
</TEI>
<affiliations><list><country><li>Hong Kong</li>
</country>
</list>
<tree><country name="Hong Kong"><noRegion><name sortKey="Xiaofen Zhong" sort="Xiaofen Zhong" uniqKey="Xiaofen Zhong" last="Xiaofen Zhong">XIAOFEN ZHONG</name>
</noRegion>
<name sortKey="Huanghao Yang" sort="Huanghao Yang" uniqKey="Huanghao Yang" last="Huanghao Yang">HUANGHAO YANG</name>
<name sortKey="Huanghao Yang" sort="Huanghao Yang" uniqKey="Huanghao Yang" last="Huanghao Yang">HUANGHAO YANG</name>
<name sortKey="Lai, Sik To" sort="Lai, Sik To" uniqKey="Lai S" first="Sik-To" last="Lai">Sik-To Lai</name>
<name sortKey="Lisheng Peng" sort="Lisheng Peng" uniqKey="Lisheng Peng" last="Lisheng Peng">LISHENG PENG</name>
<name sortKey="Wong, Tin Yau" sort="Wong, Tin Yau" uniqKey="Wong T" first="Tin-Yau" last="Wong">Tin-Yau Wong</name>
<name sortKey="Yong Xie" sort="Yong Xie" uniqKey="Yong Xie" last="Yong Xie">YONG XIE</name>
<name sortKey="Zhihong Guo" sort="Zhihong Guo" uniqKey="Zhihong Guo" last="Zhihong Guo">ZHIHONG GUO</name>
<name sortKey="Zhihong Guo" sort="Zhihong Guo" uniqKey="Zhihong Guo" last="Zhihong Guo">ZHIHONG GUO</name>
<name sortKey="Zufeng Guo" sort="Zufeng Guo" uniqKey="Zufeng Guo" last="Zufeng Guo">ZUFENG GUO</name>
<name sortKey="Zufeng Guo" sort="Zufeng Guo" uniqKey="Zufeng Guo" last="Zufeng Guo">ZUFENG GUO</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004717 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 004717 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Merge |type= RBID |clé= Pascal:06-0099007 |texte= Amino terminus of the SARS coronavirus protein 3a elicits strong, potentially protective humoral responses in infected patients }}
![]() | This area was generated with Dilib version V0.6.33. | ![]() |