Amino terminus of the SARS coronavirus protein 3a elicits strong, potentially protective humoral responses in infected patients
Identifieur interne : 004717 ( Main/Merge ); précédent : 004716; suivant : 004718Amino terminus of the SARS coronavirus protein 3a elicits strong, potentially protective humoral responses in infected patients
Auteurs : XIAOFEN ZHONG [Hong Kong] ; ZUFENG GUO [Hong Kong] ; HUANGHAO YANG [Hong Kong] ; LISHENG PENG [Hong Kong] ; YONG XIE [Hong Kong] ; Tin-Yau Wong [Hong Kong] ; Sik-To Lai [Hong Kong] ; ZHIHONG GUO [Hong Kong]Source :
- Journal of general virology [ 0022-1317 ] ; 2006.
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Abstract
The 3a protein of severe acute respiratory syndrome (SARS)-associated coronavirus is expressed and transported to the plasma membrane in tissue cells of infected patients. Its short N-terminal ectodomain was found to elicit strong humoral responses in half of the patients who had recovered from SARS. The ectodomain-specific antibodies from the convalescent-phase plasma readily recognized and induced destruction of 3a-expressing cells in the presence of the human complement system, demonstrating their potential ability to provide immune protection by recognizing and eliminating SARS coronavirus-infected cells that express the target protein. In addition, when coupled to a carrier protein, the ectodomain peptide elicited 3a-specific antibodies in mice and rabbit at high titres. These results showed that the N terminus of the 3a protein is highly immunogenic and elicits potentially protective humoral responses in infected patients. Therefore, the short extracellular domain may be a valuable immunogen in the development of a vaccine for infectious SARS.
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University of Science and Technology</s1><s2>Clear Water Bay, Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Zufeng Guo" sort="Zufeng Guo" uniqKey="Zufeng Guo" last="Zufeng Guo">ZUFENG GUO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1><s2>Clear Water Bay, Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Biotechnology Research Institute, The Hong Kong University of Science and Technology</s1><s2>Clear Water Bay, Kowloon</s2><s3>HKG</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Huanghao Yang" sort="Huanghao Yang" uniqKey="Huanghao Yang" last="Huanghao Yang">HUANGHAO YANG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1><s2>Clear Water Bay, Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Biotechnology Research Institute, The Hong Kong University of Science and Technology</s1><s2>Clear Water Bay, Kowloon</s2><s3>HKG</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Lisheng Peng" sort="Lisheng Peng" uniqKey="Lisheng Peng" last="Lisheng Peng">LISHENG PENG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1><s2>Clear Water Bay, Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Yong Xie" sort="Yong Xie" uniqKey="Yong Xie" last="Yong Xie">YONG XIE</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Biology, The Hong Kong University of Science and Technology</s1><s2>Clear Water Bay, Kowloon</s2><s3>HKG</s3><sZ>5 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Tin Yau" sort="Wong, Tin Yau" uniqKey="Wong T" first="Tin-Yau" last="Wong">Tin-Yau Wong</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Princess Margaret Hospital</s1><s3>HKG</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Princess Margaret Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Lai, Sik To" sort="Lai, Sik To" uniqKey="Lai S" first="Sik-To" last="Lai">Sik-To Lai</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Princess Margaret Hospital</s1><s3>HKG</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Princess Margaret Hospital</wicri:noRegion></affiliation></author><author><name sortKey="Zhihong Guo" sort="Zhihong Guo" uniqKey="Zhihong Guo" last="Zhihong Guo">ZHIHONG GUO</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, The Hong Kong University of Science and Technology</s1><s2>Clear Water Bay, Kowloon</s2><s3>HKG</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion></affiliation><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Biotechnology Research Institute, The Hong Kong University of Science and Technology</s1><s2>Clear Water Bay, Kowloon</s2><s3>HKG</s3><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Hong Kong</country><wicri:noRegion>Clear Water Bay, Kowloon</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of general virology</title><title level="j" type="abbreviated">J. gen. virol.</title><idno type="ISSN">0022-1317</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of general virology</title><title level="j" type="abbreviated">J. gen. virol.</title><idno type="ISSN">0022-1317</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Human</term><term>Humoral immunity</term><term>Infection</term><term>Microbiology</term><term>Protein</term><term>Severe acute respiratory syndrome virus</term><term>Virology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term><term>Homme</term><term>Protéine</term><term>Immunité humorale</term><term>Microbiologie</term><term>Virologie</term><term>Infection</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Homme</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The 3a protein of severe acute respiratory syndrome (SARS)-associated coronavirus is expressed and transported to the plasma membrane in tissue cells of infected patients. Its short N-terminal ectodomain was found to elicit strong humoral responses in half of the patients who had recovered from SARS. The ectodomain-specific antibodies from the convalescent-phase plasma readily recognized and induced destruction of 3a-expressing cells in the presence of the human complement system, demonstrating their potential ability to provide immune protection by recognizing and eliminating SARS coronavirus-infected cells that express the target protein. In addition, when coupled to a carrier protein, the ectodomain peptide elicited 3a-specific antibodies in mice and rabbit at high titres. These results showed that the N terminus of the 3a protein is highly immunogenic and elicits potentially protective humoral responses in infected patients. Therefore, the short extracellular domain may be a valuable immunogen in the development of a vaccine for infectious SARS.</div></front></TEI><affiliations><list><country><li>Hong Kong</li></country></list><tree><country name="Hong Kong"><noRegion><name sortKey="Xiaofen Zhong" sort="Xiaofen Zhong" uniqKey="Xiaofen Zhong" last="Xiaofen Zhong">XIAOFEN ZHONG</name></noRegion><name sortKey="Huanghao Yang" sort="Huanghao Yang" uniqKey="Huanghao Yang" last="Huanghao Yang">HUANGHAO YANG</name><name sortKey="Huanghao Yang" sort="Huanghao Yang" uniqKey="Huanghao Yang" last="Huanghao Yang">HUANGHAO YANG</name><name sortKey="Lai, Sik To" sort="Lai, Sik To" uniqKey="Lai S" first="Sik-To" last="Lai">Sik-To Lai</name><name sortKey="Lisheng Peng" sort="Lisheng Peng" uniqKey="Lisheng Peng" last="Lisheng Peng">LISHENG PENG</name><name sortKey="Wong, Tin Yau" sort="Wong, Tin Yau" uniqKey="Wong T" first="Tin-Yau" last="Wong">Tin-Yau Wong</name><name sortKey="Yong Xie" sort="Yong Xie" uniqKey="Yong Xie" last="Yong Xie">YONG XIE</name><name sortKey="Zhihong Guo" sort="Zhihong Guo" uniqKey="Zhihong Guo" last="Zhihong Guo">ZHIHONG GUO</name><name sortKey="Zhihong Guo" sort="Zhihong Guo" uniqKey="Zhihong Guo" last="Zhihong Guo">ZHIHONG GUO</name><name sortKey="Zufeng Guo" sort="Zufeng Guo" uniqKey="Zufeng Guo" last="Zufeng Guo">ZUFENG GUO</name><name sortKey="Zufeng Guo" sort="Zufeng Guo" uniqKey="Zufeng Guo" last="Zufeng Guo">ZUFENG GUO</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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