Detection of severe acute respiratory syndrome coronavirus in stool specimens by commercially available real-time reverse transcriptase PCR assays.
Identifieur interne : 004127 ( Main/Merge ); précédent : 004126; suivant : 004128Detection of severe acute respiratory syndrome coronavirus in stool specimens by commercially available real-time reverse transcriptase PCR assays.
Auteurs : L. Louie [Canada] ; A E Simor ; S. Chong ; K. Luinstra ; A. Petrich ; J. Mahony ; M. Smieja ; G. Johnson ; F. Gharabaghi ; R. Tellier ; B M Willey ; S. Poutanen ; T. Mazzulli ; G. Broukhanski ; F. Jamieson ; M. Louie ; S. RichardsonSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2006.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Virus du SRAS.
- isolement et purification : ARN viral, Virus du SRAS.
- virologie : Fèces.
- Humains, RT-PCR, Sensibilité et spécificité.
English descriptors
- KwdEn :
- MESH :
- chemical , isolation & purification : RNA, Viral.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Feces.
- Humans, Sensitivity and Specificity.
Abstract
Three commercially available real-time reverse transcriptase PCR assays (the Artus RealArt HPA coronavirus LightCycler, the Artus RealArt HPA coronavirus Rotor-Gene, and the EraGen severe acute respiratory syndrome coronavirus POL assay) and three RNA extraction methodologies were evaluated for the detection of severe acute respiratory syndrome coronavirus RNA from 91 stool specimens. The assays' sensitivities were highest (58% to 75%) for specimens obtained 8 to 21 days after symptom onset. The assays were less sensitive when specimens were obtained less than 8 days or more than 21 days after the onset of symptoms. All assays were 100% specific.
DOI: 10.1128/JCM.01202-06
PubMed: 16943352
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pubmed:16943352Le document en format XML
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Louie</name><affiliation wicri:level="1"><nlm:affiliation>Department of Microbiology, Sunnybrook Health Sciences Centre, B121-2075 Bayview Ave., Toronto, Ontario, Canada M4N 3M5. lisa.louie@sunnybrook.ca</nlm:affiliation><country>Canada</country><wicri:regionArea>Department of Microbiology, Sunnybrook Health Sciences Centre, B121-2075 Bayview Ave., Toronto, Ontario</wicri:regionArea><wicri:noRegion>Ontario</wicri:noRegion></affiliation></author><author><name sortKey="Simor, A E" sort="Simor, A E" uniqKey="Simor A" first="A E" last="Simor">A E Simor</name></author><author><name sortKey="Chong, S" sort="Chong, S" uniqKey="Chong S" first="S" last="Chong">S. Chong</name></author><author><name sortKey="Luinstra, K" sort="Luinstra, K" uniqKey="Luinstra K" first="K" last="Luinstra">K. Luinstra</name></author><author><name sortKey="Petrich, A" sort="Petrich, A" uniqKey="Petrich A" first="A" last="Petrich">A. 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Richardson</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Feces (virology)</term><term>Humans</term><term>RNA, Viral (isolation & purification)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (isolement et purification)</term><term>Fèces (virologie)</term><term>Humains</term><term>RT-PCR ()</term><term>Sensibilité et spécificité</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fèces</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>RT-PCR</term><term>Sensibilité et spécificité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Three commercially available real-time reverse transcriptase PCR assays (the Artus RealArt HPA coronavirus LightCycler, the Artus RealArt HPA coronavirus Rotor-Gene, and the EraGen severe acute respiratory syndrome coronavirus POL assay) and three RNA extraction methodologies were evaluated for the detection of severe acute respiratory syndrome coronavirus RNA from 91 stool specimens. The assays' sensitivities were highest (58% to 75%) for specimens obtained 8 to 21 days after symptom onset. The assays were less sensitive when specimens were obtained less than 8 days or more than 21 days after the onset of symptoms. All assays were 100% specific.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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