Detection of severe acute respiratory syndrome coronavirus in stool specimens by commercially available real-time reverse transcriptase PCR assays.
Identifieur interne : 002106 ( PubMed/Corpus ); précédent : 002105; suivant : 002107Detection of severe acute respiratory syndrome coronavirus in stool specimens by commercially available real-time reverse transcriptase PCR assays.
Auteurs : L. Louie ; A E Simor ; S. Chong ; K. Luinstra ; A. Petrich ; J. Mahony ; M. Smieja ; G. Johnson ; F. Gharabaghi ; R. Tellier ; B M Willey ; S. Poutanen ; T. Mazzulli ; G. Broukhanski ; F. Jamieson ; M. Louie ; S. RichardsonSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2006.
English descriptors
- KwdEn :
- MESH :
- chemical , isolation & purification : RNA, Viral.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Feces.
- Humans, Sensitivity and Specificity.
Abstract
Three commercially available real-time reverse transcriptase PCR assays (the Artus RealArt HPA coronavirus LightCycler, the Artus RealArt HPA coronavirus Rotor-Gene, and the EraGen severe acute respiratory syndrome coronavirus POL assay) and three RNA extraction methodologies were evaluated for the detection of severe acute respiratory syndrome coronavirus RNA from 91 stool specimens. The assays' sensitivities were highest (58% to 75%) for specimens obtained 8 to 21 days after symptom onset. The assays were less sensitive when specimens were obtained less than 8 days or more than 21 days after the onset of symptoms. All assays were 100% specific.
DOI: 10.1128/JCM.01202-06
PubMed: 16943352
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pubmed:16943352Le document en format XML
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Luinstra</name></author><author><name sortKey="Petrich, A" sort="Petrich, A" uniqKey="Petrich A" first="A" last="Petrich">A. Petrich</name></author><author><name sortKey="Mahony, J" sort="Mahony, J" uniqKey="Mahony J" first="J" last="Mahony">J. Mahony</name></author><author><name sortKey="Smieja, M" sort="Smieja, M" uniqKey="Smieja M" first="M" last="Smieja">M. Smieja</name></author><author><name sortKey="Johnson, G" sort="Johnson, G" uniqKey="Johnson G" first="G" last="Johnson">G. Johnson</name></author><author><name sortKey="Gharabaghi, F" sort="Gharabaghi, F" uniqKey="Gharabaghi F" first="F" last="Gharabaghi">F. Gharabaghi</name></author><author><name sortKey="Tellier, R" sort="Tellier, R" uniqKey="Tellier R" first="R" last="Tellier">R. Tellier</name></author><author><name sortKey="Willey, B M" sort="Willey, B M" uniqKey="Willey B" first="B M" last="Willey">B M Willey</name></author><author><name sortKey="Poutanen, S" sort="Poutanen, S" uniqKey="Poutanen S" first="S" last="Poutanen">S. Poutanen</name></author><author><name sortKey="Mazzulli, T" sort="Mazzulli, T" uniqKey="Mazzulli T" first="T" last="Mazzulli">T. Mazzulli</name></author><author><name sortKey="Broukhanski, G" sort="Broukhanski, G" uniqKey="Broukhanski G" first="G" last="Broukhanski">G. Broukhanski</name></author><author><name sortKey="Jamieson, F" sort="Jamieson, F" uniqKey="Jamieson F" first="F" last="Jamieson">F. Jamieson</name></author><author><name sortKey="Louie, M" sort="Louie, M" uniqKey="Louie M" first="M" last="Louie">M. Louie</name></author><author><name sortKey="Richardson, S" sort="Richardson, S" uniqKey="Richardson S" first="S" last="Richardson">S. Richardson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16943352</idno><idno type="pmid">16943352</idno><idno type="doi">10.1128/JCM.01202-06</idno><idno type="wicri:Area/PubMed/Corpus">002106</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002106</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Detection of severe acute respiratory syndrome coronavirus in stool specimens by commercially available real-time reverse transcriptase PCR assays.</title><author><name sortKey="Louie, L" sort="Louie, L" uniqKey="Louie L" first="L" last="Louie">L. Louie</name><affiliation><nlm:affiliation>Department of Microbiology, Sunnybrook Health Sciences Centre, B121-2075 Bayview Ave., Toronto, Ontario, Canada M4N 3M5. lisa.louie@sunnybrook.ca</nlm:affiliation></affiliation></author><author><name sortKey="Simor, A E" sort="Simor, A E" uniqKey="Simor A" first="A E" last="Simor">A E Simor</name></author><author><name sortKey="Chong, S" sort="Chong, S" uniqKey="Chong S" first="S" last="Chong">S. Chong</name></author><author><name sortKey="Luinstra, K" sort="Luinstra, K" uniqKey="Luinstra K" first="K" last="Luinstra">K. Luinstra</name></author><author><name sortKey="Petrich, A" sort="Petrich, A" uniqKey="Petrich A" first="A" last="Petrich">A. Petrich</name></author><author><name sortKey="Mahony, J" sort="Mahony, J" uniqKey="Mahony J" first="J" last="Mahony">J. Mahony</name></author><author><name sortKey="Smieja, M" sort="Smieja, M" uniqKey="Smieja M" first="M" last="Smieja">M. Smieja</name></author><author><name sortKey="Johnson, G" sort="Johnson, G" uniqKey="Johnson G" first="G" last="Johnson">G. Johnson</name></author><author><name sortKey="Gharabaghi, F" sort="Gharabaghi, F" uniqKey="Gharabaghi F" first="F" last="Gharabaghi">F. Gharabaghi</name></author><author><name sortKey="Tellier, R" sort="Tellier, R" uniqKey="Tellier R" first="R" last="Tellier">R. Tellier</name></author><author><name sortKey="Willey, B M" sort="Willey, B M" uniqKey="Willey B" first="B M" last="Willey">B M Willey</name></author><author><name sortKey="Poutanen, S" sort="Poutanen, S" uniqKey="Poutanen S" first="S" last="Poutanen">S. Poutanen</name></author><author><name sortKey="Mazzulli, T" sort="Mazzulli, T" uniqKey="Mazzulli T" first="T" last="Mazzulli">T. Mazzulli</name></author><author><name sortKey="Broukhanski, G" sort="Broukhanski, G" uniqKey="Broukhanski G" first="G" last="Broukhanski">G. 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Richardson</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Feces (virology)</term><term>Humans</term><term>RNA, Viral (isolation & purification)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Three commercially available real-time reverse transcriptase PCR assays (the Artus RealArt HPA coronavirus LightCycler, the Artus RealArt HPA coronavirus Rotor-Gene, and the EraGen severe acute respiratory syndrome coronavirus POL assay) and three RNA extraction methodologies were evaluated for the detection of severe acute respiratory syndrome coronavirus RNA from 91 stool specimens. The assays' sensitivities were highest (58% to 75%) for specimens obtained 8 to 21 days after symptom onset. The assays were less sensitive when specimens were obtained less than 8 days or more than 21 days after the onset of symptoms. All assays were 100% specific.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16943352</PMID><DateCompleted><Year>2006</Year><Month>12</Month><Day>22</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0095-1137</ISSN><JournalIssue CitedMedium="Print"><Volume>44</Volume><Issue>11</Issue><PubDate><Year>2006</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Detection of severe acute respiratory syndrome coronavirus in stool specimens by commercially available real-time reverse transcriptase PCR assays.</ArticleTitle><Pagination><MedlinePgn>4193-6</MedlinePgn></Pagination><Abstract><AbstractText>Three commercially available real-time reverse transcriptase PCR assays (the Artus RealArt HPA coronavirus LightCycler, the Artus RealArt HPA coronavirus Rotor-Gene, and the EraGen severe acute respiratory syndrome coronavirus POL assay) and three RNA extraction methodologies were evaluated for the detection of severe acute respiratory syndrome coronavirus RNA from 91 stool specimens. The assays' sensitivities were highest (58% to 75%) for specimens obtained 8 to 21 days after symptom onset. The assays were less sensitive when specimens were obtained less than 8 days or more than 21 days after the onset of symptoms. All assays were 100% specific.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Louie</LastName><ForeName>L</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Sunnybrook Health Sciences Centre, B121-2075 Bayview Ave., Toronto, Ontario, Canada M4N 3M5. lisa.louie@sunnybrook.ca</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Simor</LastName><ForeName>A E</ForeName><Initials>AE</Initials></Author><Author ValidYN="Y"><LastName>Chong</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Luinstra</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Petrich</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Mahony</LastName><ForeName>J</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Smieja</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Johnson</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Gharabaghi</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Tellier</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Willey</LastName><ForeName>B M</ForeName><Initials>BM</Initials></Author><Author ValidYN="Y"><LastName>Poutanen</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Mazzulli</LastName><ForeName>T</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Broukhanski</LastName><ForeName>G</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Jamieson</LastName><ForeName>F</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>Louie</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Richardson</LastName><ForeName>S</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><CollectiveName>Ontario Laboratory Working Group for the Rapid Diagnosis of Emerging Pathogens</CollectiveName></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2006</Year><Month>08</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Clin Microbiol</MedlineTA><NlmUniqueID>7505564</NlmUniqueID><ISSNLinking>0095-1137</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D005243" 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