Molecular Diagnosis of Severe Acute Respiratory Syndrome
Identifieur interne : 003F97 ( Main/Merge ); précédent : 003F96; suivant : 003F98Molecular Diagnosis of Severe Acute Respiratory Syndrome
Auteurs :Source :
- Clinical Applications of PCR ; 2006.
Descripteurs français
- KwdFr :
- ARN (métabolisme), ARN (sang), ARN viral (métabolisme), Humains, Modèles génétiques, RT-PCR, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (génétique), Syndrome respiratoire aigu sévère (virologie), Techniques d'amplification d'acides nucléiques, Techniques de diagnostic moléculaire (), Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Syndrome respiratoire aigu sévère, Virus du SRAS.
- métabolisme : ARN, ARN viral, Virus du SRAS.
- sang : ARN.
- virologie : Syndrome respiratoire aigu sévère.
- Humains, Modèles génétiques, RT-PCR, Techniques d'amplification d'acides nucléiques, Techniques de diagnostic moléculaire.
English descriptors
- KwdEn :
- Humans, Models, Genetic, Molecular Diagnostic Techniques (methods), Nucleic Acid Amplification Techniques, RNA (blood), RNA (metabolism), RNA, Viral (metabolism), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (metabolism), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (genetics), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , blood : RNA.
- chemical , metabolism : RNA, RNA, Viral.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus, Severe Acute Respiratory Syndrome.
- metabolism : SARS Virus.
- methods : Molecular Diagnostic Techniques.
- virology : Severe Acute Respiratory Syndrome.
- Humans, Models, Genetic, Nucleic Acid Amplification Techniques, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
The etiologic agent of severe acute respiratory syndrome (SARS) has been identified as a new type of coronavirus, known as SARS-coronavirus (SARS-CoV). Although the SARS epidemic has subsided, many authorities, including the World Health Organization (WHO) and the Centers for Disease Control and Prevention (CDC), have warned of the possible re-emergence of this highly infectious disease. Although antibody-based diagnosis of SARS has been demonstrated to be a reliable proof of SARS infection, it is not sensitive enough for detection during the early phase of the disease. To date, based on the publicly released full genomic sequences of SARS-CoV, various molecular detection methods based on reverse-transcription polymerase chain reaction (RT-PCR) have been developed. Although most of the assays have initially been focused on RNA extracted from nasopharyngeal aspirates, urine, and stools, several of the more recently developed assays have been based on the analysis of RNA extracted from plasma and serum. Such assays allow the more standardized quantitative expression of viral loads and are potentially useful for early SARS diagnosis. In this chapter, two real-time quantitative RT-PCR systems for the quantification of SARS-CoV RNA in serum are discussed. The two RT-PCR systems, one aimed toward the nucleocapsid region and the other toward the polymerase region of the virus genome, have a detection rate of up to 80% during the first week of illness. These quantitative systems are potentially useful for the early diagnosis of SARS and can also provide viral load information that might assist clinicians in making a prognostic evaluation of an infected individual.
Url:
DOI: 10.1385/1-59745-074-X:163
PubMed: 16916262
PubMed Central: 7121026
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000098
- to stream Pmc, to step Curation: 000098
- to stream Pmc, to step Checkpoint: 001097
- to stream PubMed, to step Corpus: 002121
- to stream PubMed, to step Curation: 002121
- to stream PubMed, to step Checkpoint: 002106
- to stream Ncbi, to step Merge: 001625
- to stream Ncbi, to step Curation: 001625
- to stream Ncbi, to step Checkpoint: 001625
Links to Exploration step
PMC:7121026Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Molecular Diagnosis of Severe Acute Respiratory Syndrome</title></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16916262</idno><idno type="pmc">7121026</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7121026</idno><idno type="RBID">PMC:7121026</idno><idno type="doi">10.1385/1-59745-074-X:163</idno><date when="2006">2006</date><idno type="wicri:Area/Pmc/Corpus">000098</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000098</idno><idno type="wicri:Area/Pmc/Curation">000098</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000098</idno><idno type="wicri:Area/Pmc/Checkpoint">001097</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001097</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:16916262</idno><idno type="wicri:Area/PubMed/Corpus">002121</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002121</idno><idno type="wicri:Area/PubMed/Curation">002121</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002121</idno><idno type="wicri:Area/PubMed/Checkpoint">002106</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002106</idno><idno type="wicri:Area/Ncbi/Merge">001625</idno><idno type="wicri:Area/Ncbi/Curation">001625</idno><idno type="wicri:Area/Ncbi/Checkpoint">001625</idno><idno type="wicri:Area/Main/Merge">003F97</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Molecular Diagnosis of Severe Acute Respiratory Syndrome</title></analytic><series><title level="j">Clinical Applications of PCR</title><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term><term>Models, Genetic</term><term>Molecular Diagnostic Techniques (methods)</term><term>Nucleic Acid Amplification Techniques</term><term>RNA (blood)</term><term>RNA (metabolism)</term><term>RNA, Viral (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (genetics)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN (métabolisme)</term><term>ARN (sang)</term><term>ARN viral (métabolisme)</term><term>Humains</term><term>Modèles génétiques</term><term>RT-PCR</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (génétique)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Techniques d'amplification d'acides nucléiques</term><term>Techniques de diagnostic moléculaire ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>RNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>RNA</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN</term><term>ARN viral</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>ARN</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Models, Genetic</term><term>Nucleic Acid Amplification Techniques</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Modèles génétiques</term><term>RT-PCR</term><term>Techniques d'amplification d'acides nucléiques</term><term>Techniques de diagnostic moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The etiologic agent of severe acute respiratory syndrome (SARS) has been identified as a new type of coronavirus, known as SARS-coronavirus (SARS-CoV). Although the SARS epidemic has subsided, many authorities, including the World Health Organization (WHO) and the Centers for Disease Control and Prevention (CDC), have warned of the possible re-emergence of this highly infectious disease. Although antibody-based diagnosis of SARS has been demonstrated to be a reliable proof of SARS infection, it is not sensitive enough for detection during the early phase of the disease. To date, based on the publicly released full genomic sequences of SARS-CoV, various molecular detection methods based on reverse-transcription polymerase chain reaction (RT-PCR) have been developed. Although most of the assays have initially been focused on RNA extracted from nasopharyngeal aspirates, urine, and stools, several of the more recently developed assays have been based on the analysis of RNA extracted from plasma and serum. Such assays allow the more standardized quantitative expression of viral loads and are potentially useful for early SARS diagnosis. In this chapter, two real-time quantitative RT-PCR systems for the quantification of SARS-CoV RNA in serum are discussed. The two RT-PCR systems, one aimed toward the nucleocapsid region and the other toward the polymerase region of the virus genome, have a detection rate of up to 80% during the first week of illness. These quantitative systems are potentially useful for the early diagnosis of SARS and can also provide viral load information that might assist clinicians in making a prognostic evaluation of an infected individual.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J. S. Peiris</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y. Guan</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K. Y. Yuen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C. Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S. Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W. Preiser</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J. S. Peiris</name></author><author><name sortKey="Lai, S T" uniqKey="Lai S">S. T. Lai</name></author><author><name sortKey="Poon, L L M" uniqKey="Poon L">L. L. M. Poon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poutanen, S M" uniqKey="Poutanen S">S. M. Poutanen</name></author><author><name sortKey="Low, D E" uniqKey="Low D">D. E. Low</name></author><author><name sortKey="Henry, B" uniqKey="Henry B">B. Henry</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fouchier, R A" uniqKey="Fouchier R">R. A. Fouchier</name></author><author><name sortKey="Kuiken, T" uniqKey="Kuiken T">T. Kuiken</name></author><author><name sortKey="Schutten, M" uniqKey="Schutten M">M. Schutten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuiken, T" uniqKey="Kuiken T">T. Kuiken</name></author><author><name sortKey="Fouchier, R A M" uniqKey="Fouchier R">R. A. M. Fouchier</name></author><author><name sortKey="Schutten, M" uniqKey="Schutten M">M. Schutten</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J. S. Peiris</name></author><author><name sortKey="Chu, C M" uniqKey="Chu C">C. M. Chu</name></author><author><name sortKey="Cheng, V C" uniqKey="Cheng V">V. C. Cheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tsui, S K W" uniqKey="Tsui S">S. K. W. Tsui</name></author><author><name sortKey="Chim, S S C" uniqKey="Chim S">S. S. C. Chim</name></author><author><name sortKey="Lo, Y M D" uniqKey="Lo Y">Y. M. D. Lo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rota, P A" uniqKey="Rota P">P. A. Rota</name></author><author><name sortKey="Oberste, M S" uniqKey="Oberste M">M. S. Oberste</name></author><author><name sortKey="Monroe, S S" uniqKey="Monroe S">S. S. Monroe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marra, M A" uniqKey="Marra M">M. A. Marra</name></author><author><name sortKey="Jones, S J" uniqKey="Jones S">S. J. Jones</name></author><author><name sortKey="Astell, C R" uniqKey="Astell C">C. R. Astell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poon, L L M" uniqKey="Poon L">L. L. M. Poon</name></author><author><name sortKey="Wong, O K" uniqKey="Wong O">O. K. Wong</name></author><author><name sortKey="Luk, W" uniqKey="Luk W">W. Luk</name></author><author><name sortKey="Yuen, K Y" uniqKey="Yuen K">K. Y. Yuen</name></author><author><name sortKey="Peiris, J S" uniqKey="Peiris J">J. S. Peiris</name></author><author><name sortKey="Guan, Y" uniqKey="Guan Y">Y. Guan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poon, L L M" uniqKey="Poon L">L. L. M. Poon</name></author><author><name sortKey="Chan, K H" uniqKey="Chan K">K. H. Chan</name></author><author><name sortKey="Wong, O K" uniqKey="Wong O">O. K. Wong</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ng, E K O" uniqKey="Ng E">E. K. O. Ng</name></author><author><name sortKey="Hui, D S C" uniqKey="Hui D">D. S. C. Hui</name></author><author><name sortKey="Chan, K C A" uniqKey="Chan K">K. C. A. Chan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ng, E K O" uniqKey="Ng E">E. K. O. Ng</name></author><author><name sortKey="Ng, P C" uniqKey="Ng P">P. C. Ng</name></author><author><name sortKey="Hon, K L" uniqKey="Hon K">K. L. Hon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hung, E C W" uniqKey="Hung E">E. C. W. Hung</name></author><author><name sortKey="Chim, S S C" uniqKey="Chim S">S. S. C. Chim</name></author><author><name sortKey="Chan, P K" uniqKey="Chan P">P. K. Chan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhao, J R" uniqKey="Zhao J">J. R. Zhao</name></author><author><name sortKey="Bai, Y J" uniqKey="Bai Y">Y. J. Bai</name></author><author><name sortKey="Zhang, Q H" uniqKey="Zhang Q">Q. H. Zhang</name></author><author><name sortKey="Wan, Y" uniqKey="Wan Y">Y. Wan</name></author><author><name sortKey="Li, D" uniqKey="Li D">D. Li</name></author><author><name sortKey="Yan, X J" uniqKey="Yan X">X. J. Yan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Castelain, S" uniqKey="Castelain S">S. Castelain</name></author><author><name sortKey="Descamps, V" uniqKey="Descamps V">V. Descamps</name></author><author><name sortKey="Thibault, V" uniqKey="Thibault V">V. Thibault</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bustin, S A" uniqKey="Bustin S">S. A. Bustin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ng, E K O" uniqKey="Ng E">E. K. O. Ng</name></author><author><name sortKey="Tsui, N B Y" uniqKey="Tsui N">N. B. Y. Tsui</name></author><author><name sortKey="Lau, T K" uniqKey="Lau T">T. K. Lau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chiu, W K" uniqKey="Chiu W">W. K. Chiu</name></author><author><name sortKey="Cheung, P C" uniqKey="Cheung P">P. C. Cheung</name></author><author><name sortKey="Ng, K I" uniqKey="Ng K">K. I. Ng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hon, K L" uniqKey="Hon K">K. L. Hon</name></author><author><name sortKey="Leung, C W" uniqKey="Leung C">C. W. Leung</name></author><author><name sortKey="Cheng, W T" uniqKey="Cheng W">W. T. Cheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Borst, A" uniqKey="Borst A">A. Borst</name></author><author><name sortKey="Box, A T A" uniqKey="Box A">A. T. A. Box</name></author><author><name sortKey="Fluit, A C" uniqKey="Fluit A">A. C. Fluit</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Longo, M C" uniqKey="Longo M">M. C. Longo</name></author><author><name sortKey="Berninger, M S" uniqKey="Berninger M">M. S. Berninger</name></author><author><name sortKey="Hartley, J L" uniqKey="Hartley J">J. L. Hartley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gibson, U E" uniqKey="Gibson U">U. E. Gibson</name></author><author><name sortKey="Heid, C A" uniqKey="Heid C">C. A. Heid</name></author><author><name sortKey="Williams, P M" uniqKey="Williams P">P. M. Williams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Myers, T W" uniqKey="Myers T">T. W. Myers</name></author><author><name sortKey="Gelfand, D H" uniqKey="Gelfand D">D. H. Gelfand</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tsui, N B Y" uniqKey="Tsui N">N. B. Y. Tsui</name></author><author><name sortKey="Ng, E K O" uniqKey="Ng E">E. K. O. Ng</name></author><author><name sortKey="Lo, Y M D" uniqKey="Lo Y">Y. M. D. Lo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poddar, S K" uniqKey="Poddar S">S. K. Poddar</name></author><author><name sortKey="Sawyer, M H" uniqKey="Sawyer M">M. H. Sawyer</name></author><author><name sortKey="Connor, J D" uniqKey="Connor J">J. D. Connor</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003F97 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 003F97 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Merge
|type= RBID
|clé= PMC:7121026
|texte= Molecular Diagnosis of Severe Acute Respiratory Syndrome
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:16916262" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |