[Enhanced-real time PCR: a highly sensitive method for SARS-coronavirus detection].
Identifieur interne : 003D78 ( Main/Merge ); précédent : 003D77; suivant : 003D79[Enhanced-real time PCR: a highly sensitive method for SARS-coronavirus detection].
Auteurs : Chang-Hai Yu [République populaire de Chine] ; Le-Ting Liu ; Shuang Liu ; Yan-Yun Feng ; Chen-Ran Wang ; Hui-Li Li ; Chen Wang ; Ji-Sheng HanSource :
- Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences [ 1671-167X ] ; 2006.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ARN viral.
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- Humains, Limite de détection, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Real-Time Polymerase Chain Reaction.
- Humans, Limit of Detection, Sensitivity and Specificity.
Abstract
An enhanced real-time polymerase chain reaction (ERT-PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-Cov) has been designed for detection of SARS-Cov with high sensitivity and easy-to-interpret results, in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. The limit of detection of the ERT-PCR method was 10(-2) higher than the standard real-time PCR assay and 10(-7) higher than conventional PCR methods. The increased sensitivity of the assay would have major benefits in screening suspected SARS patients rapidly and efficiently and may help control the spread of SARS and other infectious diseases during future outbreak.
PubMed: 16617369
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pubmed:16617369Le document en format XML
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<author><name sortKey="Yu, Chang Hai" sort="Yu, Chang Hai" uniqKey="Yu C" first="Chang-Hai" last="Yu">Chang-Hai Yu</name>
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<author><name sortKey="Liu, Le Ting" sort="Liu, Le Ting" uniqKey="Liu L" first="Le-Ting" last="Liu">Le-Ting Liu</name>
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<author><name sortKey="Liu, Shuang" sort="Liu, Shuang" uniqKey="Liu S" first="Shuang" last="Liu">Shuang Liu</name>
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<author><name sortKey="Wang, Chen Ran" sort="Wang, Chen Ran" uniqKey="Wang C" first="Chen-Ran" last="Wang">Chen-Ran Wang</name>
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<author><name sortKey="Li, Hui Li" sort="Li, Hui Li" uniqKey="Li H" first="Hui-Li" last="Li">Hui-Li Li</name>
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<author><name sortKey="Wang, Chen" sort="Wang, Chen" uniqKey="Wang C" first="Chen" last="Wang">Chen Wang</name>
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<author><name sortKey="Liu, Shuang" sort="Liu, Shuang" uniqKey="Liu S" first="Shuang" last="Liu">Shuang Liu</name>
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<author><name sortKey="Feng, Yan Yun" sort="Feng, Yan Yun" uniqKey="Feng Y" first="Yan-Yun" last="Feng">Yan-Yun Feng</name>
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<author><name sortKey="Wang, Chen Ran" sort="Wang, Chen Ran" uniqKey="Wang C" first="Chen-Ran" last="Wang">Chen-Ran Wang</name>
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<author><name sortKey="Li, Hui Li" sort="Li, Hui Li" uniqKey="Li H" first="Hui-Li" last="Li">Hui-Li Li</name>
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<author><name sortKey="Wang, Chen" sort="Wang, Chen" uniqKey="Wang C" first="Chen" last="Wang">Chen Wang</name>
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<author><name sortKey="Han, Ji Sheng" sort="Han, Ji Sheng" uniqKey="Han J" first="Ji-Sheng" last="Han">Ji-Sheng Han</name>
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<series><title level="j">Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences</title>
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<term>Real-Time Polymerase Chain Reaction (methods)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Sensitivity and Specificity</term>
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<term>Limite de détection</term>
<term>Réaction de polymérisation en chaine en temps réel ()</term>
<term>Sensibilité et spécificité</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (isolement et purification)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term>
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<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
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<term>Limit of Detection</term>
<term>Sensitivity and Specificity</term>
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<term>Limite de détection</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
<term>Sensibilité et spécificité</term>
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<front><div type="abstract" xml:lang="en">An enhanced real-time polymerase chain reaction (ERT-PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-Cov) has been designed for detection of SARS-Cov with high sensitivity and easy-to-interpret results, in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. The limit of detection of the ERT-PCR method was 10(-2) higher than the standard real-time PCR assay and 10(-7) higher than conventional PCR methods. The increased sensitivity of the assay would have major benefits in screening suspected SARS patients rapidly and efficiently and may help control the spread of SARS and other infectious diseases during future outbreak.</div>
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