SrasV1, PubMed, Curation, bibRecord, 002272

[Enhanced-real time PCR: a highly sensitive method for SARS-coronavirus detection].

Identifieur interne : 002272 ( PubMed/Curation ); précédent : 002271; suivant : 002273

[Enhanced-real time PCR: a highly sensitive method for SARS-coronavirus detection].

Auteurs : Chang-Hai Yu [République populaire de Chine] ; Le-Ting Liu ; Shuang Liu ; Yan-Yun Feng ; Chen-Ran Wang ; Hui-Li Li ; Chen Wang ; Ji-Sheng Han

Source :

Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences [ 1671-167X ] ; 2006.

RBID : pubmed:16617369

Descripteurs français

KwdFr :
- ARN viral (analyse), Humains, Limite de détection, Réaction de polymérisation en chaine en temps réel (), Sensibilité et spécificité, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
MESH :
- analyse : ARN viral.
- génétique : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- Humains, Limite de détection, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité.

English descriptors

KwdEn :
- Humans, Limit of Detection, RNA, Viral (analysis), Real-Time Polymerase Chain Reaction (methods), SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity.
MESH :
- chemical , analysis : RNA, Viral.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Real-Time Polymerase Chain Reaction.
- Humans, Limit of Detection, Sensitivity and Specificity.

Abstract

An enhanced real-time polymerase chain reaction (ERT-PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-Cov) has been designed for detection of SARS-Cov with high sensitivity and easy-to-interpret results, in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. The limit of detection of the ERT-PCR method was 10(-2) higher than the standard real-time PCR assay and 10(-7) higher than conventional PCR methods. The increased sensitivity of the assay would have major benefits in screening suspected SARS patients rapidly and efficiently and may help control the spread of SARS and other infectious diseases during future outbreak.

PubMed: 16617369

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pubmed:16617369

Le document en format XML

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<author><name sortKey="Yu, Chang Hai" sort="Yu, Chang Hai" uniqKey="Yu C" first="Chang-Hai" last="Yu">Chang-Hai Yu</name>
<affiliation wicri:level="1"><nlm:affiliation>Neuroscience Research Institue, Peking University, Beijing 100083, China. achy@bjmu.edu.cn</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Neuroscience Research Institue, Peking University, Beijing 100083</wicri:regionArea>
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</author>
<author><name sortKey="Liu, Le Ting" sort="Liu, Le Ting" uniqKey="Liu L" first="Le-Ting" last="Liu">Le-Ting Liu</name>
</author>
<author><name sortKey="Liu, Shuang" sort="Liu, Shuang" uniqKey="Liu S" first="Shuang" last="Liu">Shuang Liu</name>
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<author><name sortKey="Feng, Yan Yun" sort="Feng, Yan Yun" uniqKey="Feng Y" first="Yan-Yun" last="Feng">Yan-Yun Feng</name>
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<author><name sortKey="Wang, Chen Ran" sort="Wang, Chen Ran" uniqKey="Wang C" first="Chen-Ran" last="Wang">Chen-Ran Wang</name>
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<author><name sortKey="Li, Hui Li" sort="Li, Hui Li" uniqKey="Li H" first="Hui-Li" last="Li">Hui-Li Li</name>
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<author><name sortKey="Wang, Chen" sort="Wang, Chen" uniqKey="Wang C" first="Chen" last="Wang">Chen Wang</name>
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<author><name sortKey="Han, Ji Sheng" sort="Han, Ji Sheng" uniqKey="Han J" first="Ji-Sheng" last="Han">Ji-Sheng Han</name>
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<author><name sortKey="Yu, Chang Hai" sort="Yu, Chang Hai" uniqKey="Yu C" first="Chang-Hai" last="Yu">Chang-Hai Yu</name>
<affiliation wicri:level="1"><nlm:affiliation>Neuroscience Research Institue, Peking University, Beijing 100083, China. achy@bjmu.edu.cn</nlm:affiliation>
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<author><name sortKey="Feng, Yan Yun" sort="Feng, Yan Yun" uniqKey="Feng Y" first="Yan-Yun" last="Feng">Yan-Yun Feng</name>
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<author><name sortKey="Wang, Chen" sort="Wang, Chen" uniqKey="Wang C" first="Chen" last="Wang">Chen Wang</name>
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<author><name sortKey="Han, Ji Sheng" sort="Han, Ji Sheng" uniqKey="Han J" first="Ji-Sheng" last="Han">Ji-Sheng Han</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Humans</term>
<term>Limit of Detection</term>
<term>RNA, Viral (analysis)</term>
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<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term>
<term>Humains</term>
<term>Limite de détection</term>
<term>Réaction de polymérisation en chaine en temps réel ()</term>
<term>Sensibilité et spécificité</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (isolement et purification)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term>
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</keywords>
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<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
<term>Limit of Detection</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Humains</term>
<term>Limite de détection</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
<term>Sensibilité et spécificité</term>
</keywords>
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<front><div type="abstract" xml:lang="en">An enhanced real-time polymerase chain reaction (ERT-PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-Cov) has been designed for detection of SARS-Cov with high sensitivity and easy-to-interpret results, in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. The limit of detection of the ERT-PCR method was 10(-2) higher than the standard real-time PCR assay and 10(-7) higher than conventional PCR methods. The increased sensitivity of the assay would have major benefits in screening suspected SARS patients rapidly and efficiently and may help control the spread of SARS and other infectious diseases during future outbreak.</div>
</front>
</TEI>
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<Month>03</Month>
<Day>20</Day>
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<DateRevised><Year>2017</Year>
<Month>11</Month>
<Day>16</Day>
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<Title>Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences</Title>
<ISOAbbreviation>Beijing Da Xue Xue Bao</ISOAbbreviation>
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<ArticleTitle>[Enhanced-real time PCR: a highly sensitive method for SARS-coronavirus detection].</ArticleTitle>
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<Abstract><AbstractText>An enhanced real-time polymerase chain reaction (ERT-PCR) assay to detect the coronavirus associated with severe acute respiratory syndrome (SARS-Cov) has been designed for detection of SARS-Cov with high sensitivity and easy-to-interpret results, in which a target gene pre-amplification step preceded TaqMan real-time fluorescent PCR. The limit of detection of the ERT-PCR method was 10(-2) higher than the standard real-time PCR assay and 10(-7) higher than conventional PCR methods. The increased sensitivity of the assay would have major benefits in screening suspected SARS patients rapidly and efficiently and may help control the spread of SARS and other infectious diseases during future outbreak.</AbstractText>
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<ForeName>Chang-hai</ForeName>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
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<MeshHeading><DescriptorName UI="D057230" MajorTopicYN="N">Limit of Detection</DescriptorName>
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[Enhanced-real time PCR: a highly sensitive method for SARS-coronavirus detection].

[Enhanced-real time PCR: a highly sensitive method for SARS-coronavirus detection].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki