Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-XL protein
Identifieur interne : 003D18 ( Main/Merge ); précédent : 003D17; suivant : 003D19Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-XL protein
Auteurs : Ying-Xim Tan [Singapour] ; Timothy H. P. Tan [Singapour] ; Marvin J.-R. Lee [Singapour] ; Puay-Yoke Tham [Singapour] ; Vithiagaran Gunalan [Singapour] ; Julian Druce [Australie] ; Chris Birch [Australie] ; Mike Catton [Australie] ; NAI YANG FU [Singapour] ; Victor C. Yu [Singapour] ; Yee-Joo Tan [Singapour]Source :
- Journal of virology [ 0022-538X ] ; 2007.
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- Pascal (Inist)
English descriptors
Abstract
The severe acute respiratory syndrome coronavirus (SARS-CoV) 7a protein, which is not expressed by other known coronaviruses, can induce apoptosis in various cell lines. In this study, we show that the overexpression of Bcl-XL, a prosurvival member of the Bcl-2 family, blocks 7a-induced apoptosis, suggesting that the mechanism for apoptosis induction by 7a is at the level of or upstream from the Bcl-2 family. Coimmunoprecipitation experiments showed that 7a interacts with Bcl-XL and other prosurvival proteins (Bcl-2, Bcl-w, Mcl-1, and Al) but not with the proapoptotic proteins (Bax, Bak, Bad, and Bid). A good correlation between the abilities of 7a deletion mutants to induce apoptosis and to interact with Bcl-XL was observed, suggesting that 7a triggers apoptosis by interfering directly with the prosurvival function of Bcl-XL. Interestingly, amino acids 224 and 225 within the C-terminal transmembrane domain of Bcl-XL are essential for the interaction with the 7a protein, although the BH3 domain of Bcl-XL also contributes to this interaction. In addition, fractionation experiments showed that 7a colocalized with Bcl-XL at the endoplasmic reticulum as well as the mitochondria, suggesting that they may form complexes in different membranous compartments.
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Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Lee, Marvin J R" sort="Lee, Marvin J R" uniqKey="Lee M" first="Marvin J.-R." last="Lee">Marvin J.-R. Lee</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Gunalan, Vithiagaran" sort="Gunalan, Vithiagaran" uniqKey="Gunalan V" first="Vithiagaran" last="Gunalan">Vithiagaran Gunalan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Druce, Julian" sort="Druce, Julian" uniqKey="Druce J" first="Julian" last="Druce">Julian Druce</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Victorian Infectious Diseases Reference Laboratory</s1><s2>North Melbourne, Victoria</s2><s3>AUS</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Victorian Infectious Diseases Reference Laboratory</wicri:noRegion></affiliation></author><author><name sortKey="Birch, Chris" sort="Birch, Chris" uniqKey="Birch C" first="Chris" last="Birch">Chris Birch</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Victorian Infectious Diseases Reference Laboratory</s1><s2>North Melbourne, Victoria</s2><s3>AUS</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Victorian Infectious Diseases Reference Laboratory</wicri:noRegion></affiliation></author><author><name sortKey="Catton, Mike" sort="Catton, Mike" uniqKey="Catton M" first="Mike" last="Catton">Mike Catton</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Victorian Infectious Diseases Reference Laboratory</s1><s2>North Melbourne, Victoria</s2><s3>AUS</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Victorian Infectious Diseases Reference Laboratory</wicri:noRegion></affiliation></author><author><name sortKey="Nai Yang Fu" sort="Nai Yang Fu" uniqKey="Nai Yang Fu" last="Nai Yang Fu">NAI YANG FU</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Mechanisms of Apoptosis in Mammalian Cells Group, Institute of Molecular and Cell Biology</s1><s2>Singapore</s2><s3>SGP</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Victor C" sort="Yu, Victor C" uniqKey="Yu V" first="Victor C." last="Yu">Victor C. Yu</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Mechanisms of Apoptosis in Mammalian Cells Group, Institute of Molecular and Cell Biology</s1><s2>Singapore</s2><s3>SGP</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">07-0315403</idno><date when="2007">2007</date><idno type="stanalyst">PASCAL 07-0315403 INIST</idno><idno type="RBID">Pascal:07-0315403</idno><idno type="wicri:Area/PascalFrancis/Corpus">000363</idno><idno type="wicri:Area/PascalFrancis/Curation">000626</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000371</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000371</idno><idno type="wicri:doubleKey">0022-538X:2007:Tan Y:induction:of:apoptosis</idno><idno type="wicri:Area/Main/Merge">003D18</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Induction of apoptosis by the severe acute respiratory syndrome coronavirus 7a protein is dependent on its interaction with the Bcl-X<sub>L</sub> protein</title><author><name sortKey="Tan, Ying Xim" sort="Tan, Ying Xim" uniqKey="Tan Y" first="Ying-Xim" last="Tan">Ying-Xim Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Lee, Marvin J R" sort="Lee, Marvin J R" uniqKey="Lee M" first="Marvin J.-R." last="Lee">Marvin J.-R. Lee</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Gunalan, Vithiagaran" sort="Gunalan, Vithiagaran" uniqKey="Gunalan V" first="Vithiagaran" last="Gunalan">Vithiagaran Gunalan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author><author><name sortKey="Druce, Julian" sort="Druce, Julian" uniqKey="Druce J" first="Julian" last="Druce">Julian Druce</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Victorian Infectious Diseases Reference Laboratory</s1><s2>North Melbourne, Victoria</s2><s3>AUS</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Victorian Infectious Diseases Reference Laboratory</wicri:noRegion></affiliation></author><author><name sortKey="Birch, Chris" sort="Birch, Chris" uniqKey="Birch C" first="Chris" last="Birch">Chris Birch</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Victorian Infectious Diseases Reference Laboratory</s1><s2>North Melbourne, Victoria</s2><s3>AUS</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Victorian Infectious Diseases Reference Laboratory</wicri:noRegion></affiliation></author><author><name sortKey="Catton, Mike" sort="Catton, Mike" uniqKey="Catton M" first="Mike" last="Catton">Mike Catton</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Victorian Infectious Diseases Reference Laboratory</s1><s2>North Melbourne, Victoria</s2><s3>AUS</s3><sZ>6 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Australie</country><wicri:noRegion>Victorian Infectious Diseases Reference Laboratory</wicri:noRegion></affiliation></author><author><name sortKey="Nai Yang Fu" sort="Nai Yang Fu" uniqKey="Nai Yang Fu" last="Nai Yang Fu">NAI YANG FU</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Mechanisms of Apoptosis in Mammalian Cells Group, Institute of Molecular and Cell Biology</s1><s2>Singapore</s2><s3>SGP</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Victor C" sort="Yu, Victor C" uniqKey="Yu V" first="Victor C." last="Yu">Victor C. Yu</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Mechanisms of Apoptosis in Mammalian Cells Group, Institute of Molecular and Cell Biology</s1><s2>Singapore</s2><s3>SGP</s3><sZ>9 aut.</sZ><sZ>10 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore</wicri:noRegion></affiliation></author><author><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Collaborative Anti-Viral Research Group, Institute of Molecular and Cell Biology</s1><s2>Singapore </s2><s3>SGP</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ><sZ>5 aut.</sZ><sZ>11 aut.</sZ></inist:fA14><country>Singapour</country><wicri:noRegion>Singapore </wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Apoptosis</term><term>Cell death</term><term>Coronavirus</term><term>Protein</term><term>Severe acute respiratory syndrome</term><term>Virology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Apoptose</term><term>Mort cellulaire</term><term>Protéine</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome coronavirus (SARS-CoV) 7a protein, which is not expressed by other known coronaviruses, can induce apoptosis in various cell lines. In this study, we show that the overexpression of Bcl-X<sub>L</sub>, a prosurvival member of the Bcl-2 family, blocks 7a-induced apoptosis, suggesting that the mechanism for apoptosis induction by 7a is at the level of or upstream from the Bcl-2 family. Coimmunoprecipitation experiments showed that 7a interacts with Bcl-X<sub>L</sub> and other prosurvival proteins (Bcl-2, Bcl-w, Mcl-1, and Al) but not with the proapoptotic proteins (Bax, Bak, Bad, and Bid). A good correlation between the abilities of 7a deletion mutants to induce apoptosis and to interact with Bcl-X<sub>L</sub> was observed, suggesting that 7a triggers apoptosis by interfering directly with the prosurvival function of Bcl-X<sub>L</sub>. Interestingly, amino acids 224 and 225 within the C-terminal transmembrane domain of Bcl-X<sub>L</sub> are essential for the interaction with the 7a protein, although the BH3 domain of Bcl-X<sub>L</sub> also contributes to this interaction. In addition, fractionation experiments showed that 7a colocalized with Bcl-X<sub>L</sub> at the endoplasmic reticulum as well as the mitochondria, suggesting that they may form complexes in different membranous compartments.</div></front></TEI><affiliations><list><country><li>Australie</li><li>Singapour</li></country></list><tree><country name="Singapour"><noRegion><name sortKey="Tan, Ying Xim" sort="Tan, Ying Xim" uniqKey="Tan Y" first="Ying-Xim" last="Tan">Ying-Xim Tan</name></noRegion><name sortKey="Gunalan, Vithiagaran" sort="Gunalan, Vithiagaran" uniqKey="Gunalan V" first="Vithiagaran" last="Gunalan">Vithiagaran Gunalan</name><name sortKey="Lee, Marvin J R" sort="Lee, Marvin J R" uniqKey="Lee M" first="Marvin J.-R." last="Lee">Marvin J.-R. Lee</name><name sortKey="Nai Yang Fu" sort="Nai Yang Fu" uniqKey="Nai Yang Fu" last="Nai Yang Fu">NAI YANG FU</name><name sortKey="Tan, Timothy H P" sort="Tan, Timothy H P" uniqKey="Tan T" first="Timothy H. P." last="Tan">Timothy H. P. Tan</name><name sortKey="Tan, Yee Joo" sort="Tan, Yee Joo" uniqKey="Tan Y" first="Yee-Joo" last="Tan">Yee-Joo Tan</name><name sortKey="Tham, Puay Yoke" sort="Tham, Puay Yoke" uniqKey="Tham P" first="Puay-Yoke" last="Tham">Puay-Yoke Tham</name><name sortKey="Yu, Victor C" sort="Yu, Victor C" uniqKey="Yu V" first="Victor C." last="Yu">Victor C. Yu</name></country><country name="Australie"><noRegion><name sortKey="Druce, Julian" sort="Druce, Julian" uniqKey="Druce J" first="Julian" last="Druce">Julian Druce</name></noRegion><name sortKey="Birch, Chris" sort="Birch, Chris" uniqKey="Birch C" first="Chris" last="Birch">Chris Birch</name><name sortKey="Catton, Mike" sort="Catton, Mike" uniqKey="Catton M" first="Mike" last="Catton">Mike Catton</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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