SrasV1, Main, Merge, bibRecord, 003B18

Loop‐mediated isothermal amplification method for detection of human papillomavirus type 6, 11, 16, and 18

Identifieur interne : 003B18 ( Main/Merge ); précédent : 003B17; suivant : 003B19

Loop‐mediated isothermal amplification method for detection of human papillomavirus type 6, 11, 16, and 18

Auteurs : Masanori Hagiwara [Japon] ; Hajime Sasaki [Japon] ; Koma Matsuo [Japon] ; Mariko Honda [Japon] ; Masaaki Kawase [Japon] ; Hidemi Nakagawa [Japon]

Source :

Journal of Medical Virology [ 0146-6615 ] ; 2007-05.

RBID : ISTEX:2538B069494CB2B7137759C43A80E9358BCC941D

English descriptors

Teeft :
- Additional advantage, Agarose, Assay, Average reaction time, Blot hybridization, Bowenoid, Bowenoid papuloses, Bowenoid papulosis, Cervical cancer, Cervical carcinoma, Clin, Clin microbiol, Clinical samples, Condyloma, Condyloma acuminatum, Condylomata acuminata, Consensus primers, Copy number, Detection limit, Eiken chemical, Epidermolytic acanthoma, Ethidium bromide, Genital lesions, Genome quantity, Hairy nymphae, Herpes simplex virus, High copy numbers, Histologic diagnoses, Human papillomavirus, Human papillomavirus type, Human papillomavirus types, Human papillomaviruses, Isothermal, Isothermal method, Jikei aoto hospital, Jikei university school, Lamp method, Lamp primers, Lamp products, Lamp reaction, Linear correlation, Loop primers, Measles virus, Microbiol, Notomi, Papillomavirus, Papulosis, Polymerase chain reaction, Polypoid lesions, Positive samples, Primer, Quantitative method, Rapid detection, Rapid diagnosis, Reaction time, Respective type, Seborrheic keratosis, Serial dilutions, Turbidity, Turbidity assay, Valuable tool, Various types, Virol, Virological testing, Virus isolation, Yoshikawa.

Abstract

A new method was developed for detection of human papillomavirus (HPV) by loop‐mediated isothermal amplification (LAMP), which was compared with the polymerase chain reaction (PCR), and real‐time PCR for specificity and sensitivity. All initial validation studies with the control DNA proved to be type‐specific. In order to evaluate the reliability of HPV type‐specific LAMP detecting HPV DNA from clinical samples, tissue specimens were obtained from 27 patients with external genital polypoid lesions. The histologic diagnoses included condyloma acuminatum (n = 21), bowenoid papulosis (n = 2), seborrheic keratosis (n = 2), epidermolytic acanthoma (n = 1), and hairy nymphae (n = 1). HPV‐6 DNA and HPV‐11 DNA were detected in 18 and 3 of 21 condylomata acuminata, respectively, and there was no simultaneous infection. HPV‐16 DNA was detected in one of two bowenoid papuloses. HPV DNA was not detected in the seborrheic keratoses, epidermolytic acanthoma, and hairy nymphae. These results correlated perfectly with those from real‐time PCR analysis. Most positive samples contained high copy numbers of HPV DNA. HPV‐11 DNA was detected in one case that could not be detected by PCR. The average reaction time was about 59 min. There was a linear correlation between the genome quantity and reaction time to reach the threshold. The LAMP method has an additional advantage as a quantitative method, and is superior in terms of sensitivity, specificity, rapidity, and simplicity, and can potentially be a valuable tool for the detection of HPV DNA. J. Med. Virol. 79:605–615, 2007. © 2007 Wiley‐Liss, Inc.

Url:

https://api.istex.fr/ark:/67375/WNG-PKC2DLGJ-G/fulltext.pdf

DOI: 10.1002/jmv.20858

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<term>Agarose</term>
<term>Assay</term>
<term>Average reaction time</term>
<term>Blot hybridization</term>
<term>Bowenoid</term>
<term>Bowenoid papuloses</term>
<term>Bowenoid papulosis</term>
<term>Cervical cancer</term>
<term>Cervical carcinoma</term>
<term>Clin</term>
<term>Clin microbiol</term>
<term>Clinical samples</term>
<term>Condyloma</term>
<term>Condyloma acuminatum</term>
<term>Condylomata acuminata</term>
<term>Consensus primers</term>
<term>Copy number</term>
<term>Detection limit</term>
<term>Eiken chemical</term>
<term>Epidermolytic acanthoma</term>
<term>Ethidium bromide</term>
<term>Genital lesions</term>
<term>Genome quantity</term>
<term>Hairy nymphae</term>
<term>Herpes simplex virus</term>
<term>High copy numbers</term>
<term>Histologic diagnoses</term>
<term>Human papillomavirus</term>
<term>Human papillomavirus type</term>
<term>Human papillomavirus types</term>
<term>Human papillomaviruses</term>
<term>Isothermal</term>
<term>Isothermal method</term>
<term>Jikei aoto hospital</term>
<term>Jikei university school</term>
<term>Lamp method</term>
<term>Lamp primers</term>
<term>Lamp products</term>
<term>Lamp reaction</term>
<term>Linear correlation</term>
<term>Loop primers</term>
<term>Measles virus</term>
<term>Microbiol</term>
<term>Notomi</term>
<term>Papillomavirus</term>
<term>Papulosis</term>
<term>Polymerase chain reaction</term>
<term>Polypoid lesions</term>
<term>Positive samples</term>
<term>Primer</term>
<term>Quantitative method</term>
<term>Rapid detection</term>
<term>Rapid diagnosis</term>
<term>Reaction time</term>
<term>Respective type</term>
<term>Seborrheic keratosis</term>
<term>Serial dilutions</term>
<term>Turbidity</term>
<term>Turbidity assay</term>
<term>Valuable tool</term>
<term>Various types</term>
<term>Virol</term>
<term>Virological testing</term>
<term>Virus isolation</term>
<term>Yoshikawa</term>
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<front><div type="abstract" xml:lang="en">A new method was developed for detection of human papillomavirus (HPV) by loop‐mediated isothermal amplification (LAMP), which was compared with the polymerase chain reaction (PCR), and real‐time PCR for specificity and sensitivity. All initial validation studies with the control DNA proved to be type‐specific. In order to evaluate the reliability of HPV type‐specific LAMP detecting HPV DNA from clinical samples, tissue specimens were obtained from 27 patients with external genital polypoid lesions. The histologic diagnoses included condyloma acuminatum (n = 21), bowenoid papulosis (n = 2), seborrheic keratosis (n = 2), epidermolytic acanthoma (n = 1), and hairy nymphae (n = 1). HPV‐6 DNA and HPV‐11 DNA were detected in 18 and 3 of 21 condylomata acuminata, respectively, and there was no simultaneous infection. HPV‐16 DNA was detected in one of two bowenoid papuloses. HPV DNA was not detected in the seborrheic keratoses, epidermolytic acanthoma, and hairy nymphae. These results correlated perfectly with those from real‐time PCR analysis. Most positive samples contained high copy numbers of HPV DNA. HPV‐11 DNA was detected in one case that could not be detected by PCR. The average reaction time was about 59 min. There was a linear correlation between the genome quantity and reaction time to reach the threshold. The LAMP method has an additional advantage as a quantitative method, and is superior in terms of sensitivity, specificity, rapidity, and simplicity, and can potentially be a valuable tool for the detection of HPV DNA. J. Med. Virol. 79:605–615, 2007. © 2007 Wiley‐Liss, Inc.</div>
</front>
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Loop‐mediated isothermal amplification method for detection of human papillomavirus type 6, 11, 16, and 18

Loop‐mediated isothermal amplification method for detection of human papillomavirus type 6, 11, 16, and 18

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri