Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein.
Identifieur interne : 002B09 ( Main/Merge ); précédent : 002B08; suivant : 002B10Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein.
Auteurs : Lijie Tang [République populaire de Chine] ; Yijing LiSource :
- Virus genes [ 1572-994X ] ; 2009.
Descripteurs français
- KwdFr :
- Administration par voie orale, Animaux, Anticorps antiviraux (immunologie), Anticorps neutralisants (immunologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Lactococcus lactis (génétique), Muqueuse intestinale (immunologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Souris, Souris de lignée BALB C, Sérum (immunologie), Test ELISA, Tests de neutralisation, Vaccins antiviraux (administration et posologie), Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Vaccins synthétiques (génétique), Vaccins synthétiques (immunologie), Vecteurs de médicaments, Vecteurs génétiques, Virus de la gastroentérite transmissible (génétique), Virus de la gastroentérite transmissible (immunologie).
- MESH :
- administration et posologie : Vaccins antiviraux.
- génétique : Glycoprotéines membranaires, Lactococcus lactis, Protéines de l'enveloppe virale, Protéines recombinantes, Vaccins antiviraux, Vaccins synthétiques, Virus de la gastroentérite transmissible.
- immunologie : Anticorps antiviraux, Anticorps neutralisants, Glycoprotéines membranaires, Muqueuse intestinale, Protéines de l'enveloppe virale, Protéines recombinantes, Sérum, Vaccins antiviraux, Vaccins synthétiques, Virus de la gastroentérite transmissible.
- Administration par voie orale, Animaux, Glycoprotéine de spicule des coronavirus, Souris, Souris de lignée BALB C, Test ELISA, Tests de neutralisation, Vecteurs de médicaments, Vecteurs génétiques.
English descriptors
- KwdEn :
- Administration, Oral, Animals, Antibodies, Neutralizing (immunology), Antibodies, Viral (immunology), Drug Carriers, Enzyme-Linked Immunosorbent Assay, Genetic Vectors, Intestinal Mucosa (immunology), Lactococcus lactis (genetics), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Neutralization Tests, Recombinant Proteins (genetics), Recombinant Proteins (immunology), Serum (immunology), Spike Glycoprotein, Coronavirus, Transmissible gastroenteritis virus (genetics), Transmissible gastroenteritis virus (immunology), Vaccines, Synthetic (genetics), Vaccines, Synthetic (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (genetics), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Viral Vaccines.
- chemical , genetics : Membrane Glycoproteins, Recombinant Proteins, Vaccines, Synthetic, Viral Envelope Proteins, Viral Vaccines.
- chemical , immunology : Antibodies, Neutralizing, Antibodies, Viral, Membrane Glycoproteins, Recombinant Proteins, Vaccines, Synthetic, Viral Envelope Proteins, Viral Vaccines.
- genetics : Lactococcus lactis, Transmissible gastroenteritis virus.
- immunology : Intestinal Mucosa, Serum, Transmissible gastroenteritis virus.
- Administration, Oral, Animals, Drug Carriers, Enzyme-Linked Immunosorbent Assay, Genetic Vectors, Mice, Mice, Inbred BALB C, Neutralization Tests, Spike Glycoprotein, Coronavirus.
Abstract
Lactococcus lactis NZ9000 was selected as an antigen delivery vehicle for mucosal immunization against porcine transmissible gastroenteritis virus (TGEV) infection. An approximately 70 kDa fragment of the N-terminal globular domain of the spike (S) protein (SN protein) from the coronavirus TGEV was used as the transmissible gastroenteritis virus antigen model. Recombinant L. lactis, expressing the SN protein, was constructed with the pNZ8112 plasmid. Expression and localization of the transcribed SN protein from the recombinant LNZ9000- rTGEV-SN were detected via SDS-PAGE, Western blot, and immunofluorescence. BALB/c mice, orally immunized with LNZ9000-rTGEV-SN, produced local mucosal immune responses against TGEV. The induced antibodies demonstrated neutralizing effects on TGEV infection. These data indicated that the recombinant L. lactis could be a valuable tool in the development of future vaccines against TGEV.
DOI: 10.1007/s11262-009-0390-x
PubMed: 19629668
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001858
- to stream PubMed, to step Curation: 001858
- to stream PubMed, to step Checkpoint: 001824
- to stream Ncbi, to step Merge: 001F70
- to stream Ncbi, to step Curation: 001F70
- to stream Ncbi, to step Checkpoint: 001F70
Links to Exploration step
pubmed:19629668Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein.</title>
<author><name sortKey="Tang, Lijie" sort="Tang, Lijie" uniqKey="Tang L" first="Lijie" last="Tang">Lijie Tang</name>
<affiliation wicri:level="1"><nlm:affiliation>Life Science Department, Northeast Agricultural University, Harbin 150030, People's Republic of China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Life Science Department, Northeast Agricultural University, Harbin 150030</wicri:regionArea>
<wicri:noRegion>Harbin 150030</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Yijing" sort="Li, Yijing" uniqKey="Li Y" first="Yijing" last="Li">Yijing Li</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2009">2009</date>
<idno type="RBID">pubmed:19629668</idno>
<idno type="pmid">19629668</idno>
<idno type="doi">10.1007/s11262-009-0390-x</idno>
<idno type="wicri:Area/PubMed/Corpus">001858</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001858</idno>
<idno type="wicri:Area/PubMed/Curation">001858</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001858</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001824</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001824</idno>
<idno type="wicri:Area/Ncbi/Merge">001F70</idno>
<idno type="wicri:Area/Ncbi/Curation">001F70</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">001F70</idno>
<idno type="wicri:Area/Main/Merge">002B09</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein.</title>
<author><name sortKey="Tang, Lijie" sort="Tang, Lijie" uniqKey="Tang L" first="Lijie" last="Tang">Lijie Tang</name>
<affiliation wicri:level="1"><nlm:affiliation>Life Science Department, Northeast Agricultural University, Harbin 150030, People's Republic of China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Life Science Department, Northeast Agricultural University, Harbin 150030</wicri:regionArea>
<wicri:noRegion>Harbin 150030</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Yijing" sort="Li, Yijing" uniqKey="Li Y" first="Yijing" last="Li">Yijing Li</name>
</author>
</analytic>
<series><title level="j">Virus genes</title>
<idno type="eISSN">1572-994X</idno>
<imprint><date when="2009" type="published">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Administration, Oral</term>
<term>Animals</term>
<term>Antibodies, Neutralizing (immunology)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Drug Carriers</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Genetic Vectors</term>
<term>Intestinal Mucosa (immunology)</term>
<term>Lactococcus lactis (genetics)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Neutralization Tests</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Serum (immunology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Transmissible gastroenteritis virus (genetics)</term>
<term>Transmissible gastroenteritis virus (immunology)</term>
<term>Vaccines, Synthetic (genetics)</term>
<term>Vaccines, Synthetic (immunology)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Vaccines (administration & dosage)</term>
<term>Viral Vaccines (genetics)</term>
<term>Viral Vaccines (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Administration par voie orale</term>
<term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps neutralisants (immunologie)</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Lactococcus lactis (génétique)</term>
<term>Muqueuse intestinale (immunologie)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Sérum (immunologie)</term>
<term>Test ELISA</term>
<term>Tests de neutralisation</term>
<term>Vaccins antiviraux (administration et posologie)</term>
<term>Vaccins antiviraux (génétique)</term>
<term>Vaccins antiviraux (immunologie)</term>
<term>Vaccins synthétiques (génétique)</term>
<term>Vaccins synthétiques (immunologie)</term>
<term>Vecteurs de médicaments</term>
<term>Vecteurs génétiques</term>
<term>Virus de la gastroentérite transmissible (génétique)</term>
<term>Virus de la gastroentérite transmissible (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Vaccines, Synthetic</term>
<term>Viral Envelope Proteins</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Neutralizing</term>
<term>Antibodies, Viral</term>
<term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Vaccines, Synthetic</term>
<term>Viral Envelope Proteins</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Vaccins antiviraux</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Lactococcus lactis</term>
<term>Transmissible gastroenteritis virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Lactococcus lactis</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
<term>Vaccins antiviraux</term>
<term>Vaccins synthétiques</term>
<term>Virus de la gastroentérite transmissible</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Anticorps neutralisants</term>
<term>Glycoprotéines membranaires</term>
<term>Muqueuse intestinale</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
<term>Sérum</term>
<term>Vaccins antiviraux</term>
<term>Vaccins synthétiques</term>
<term>Virus de la gastroentérite transmissible</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Intestinal Mucosa</term>
<term>Serum</term>
<term>Transmissible gastroenteritis virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Administration, Oral</term>
<term>Animals</term>
<term>Drug Carriers</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Genetic Vectors</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Neutralization Tests</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Administration par voie orale</term>
<term>Animaux</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Test ELISA</term>
<term>Tests de neutralisation</term>
<term>Vecteurs de médicaments</term>
<term>Vecteurs génétiques</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Lactococcus lactis NZ9000 was selected as an antigen delivery vehicle for mucosal immunization against porcine transmissible gastroenteritis virus (TGEV) infection. An approximately 70 kDa fragment of the N-terminal globular domain of the spike (S) protein (SN protein) from the coronavirus TGEV was used as the transmissible gastroenteritis virus antigen model. Recombinant L. lactis, expressing the SN protein, was constructed with the pNZ8112 plasmid. Expression and localization of the transcribed SN protein from the recombinant LNZ9000- rTGEV-SN were detected via SDS-PAGE, Western blot, and immunofluorescence. BALB/c mice, orally immunized with LNZ9000-rTGEV-SN, produced local mucosal immune responses against TGEV. The induced antibodies demonstrated neutralizing effects on TGEV infection. These data indicated that the recombinant L. lactis could be a valuable tool in the development of future vaccines against TGEV.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002B09 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 002B09 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Merge |type= RBID |clé= pubmed:19629668 |texte= Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:19629668" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |