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Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein.

Identifieur interne : 001F70 ( Ncbi/Checkpoint ); précédent : 001F69; suivant : 001F71

Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein.

Auteurs : Lijie Tang [République populaire de Chine] ; Yijing Li

Source :

Virus genes [ 1572-994X ] ; 2009.

RBID : pubmed:19629668

Descripteurs français

English descriptors

KwdEn :
- Administration, Oral, Animals, Antibodies, Neutralizing (immunology), Antibodies, Viral (immunology), Drug Carriers, Enzyme-Linked Immunosorbent Assay, Genetic Vectors, Intestinal Mucosa (immunology), Lactococcus lactis (genetics), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Neutralization Tests, Recombinant Proteins (genetics), Recombinant Proteins (immunology), Serum (immunology), Spike Glycoprotein, Coronavirus, Transmissible gastroenteritis virus (genetics), Transmissible gastroenteritis virus (immunology), Vaccines, Synthetic (genetics), Vaccines, Synthetic (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (genetics), Viral Vaccines (immunology).
MESH :
- chemical , administration & dosage : Viral Vaccines.
- chemical , genetics : Membrane Glycoproteins, Recombinant Proteins, Vaccines, Synthetic, Viral Envelope Proteins, Viral Vaccines.
- chemical , immunology : Antibodies, Neutralizing, Antibodies, Viral, Membrane Glycoproteins, Recombinant Proteins, Vaccines, Synthetic, Viral Envelope Proteins, Viral Vaccines.
- genetics : Lactococcus lactis, Transmissible gastroenteritis virus.
- immunology : Intestinal Mucosa, Serum, Transmissible gastroenteritis virus.
- Administration, Oral, Animals, Drug Carriers, Enzyme-Linked Immunosorbent Assay, Genetic Vectors, Mice, Mice, Inbred BALB C, Neutralization Tests, Spike Glycoprotein, Coronavirus.

Abstract

Lactococcus lactis NZ9000 was selected as an antigen delivery vehicle for mucosal immunization against porcine transmissible gastroenteritis virus (TGEV) infection. An approximately 70 kDa fragment of the N-terminal globular domain of the spike (S) protein (SN protein) from the coronavirus TGEV was used as the transmissible gastroenteritis virus antigen model. Recombinant L. lactis, expressing the SN protein, was constructed with the pNZ8112 plasmid. Expression and localization of the transcribed SN protein from the recombinant LNZ9000- rTGEV-SN were detected via SDS-PAGE, Western blot, and immunofluorescence. BALB/c mice, orally immunized with LNZ9000-rTGEV-SN, produced local mucosal immune responses against TGEV. The induced antibodies demonstrated neutralizing effects on TGEV infection. These data indicated that the recombinant L. lactis could be a valuable tool in the development of future vaccines against TGEV.

DOI: 10.1007/s11262-009-0390-x
PubMed: 19629668

Affiliations:

République populaire de Chine

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pubmed:19629668

Le document en format XML

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<term>Membrane Glycoproteins (immunology)</term>
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<term>Mice, Inbred BALB C</term>
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<term>Glycoprotéines membranaires (immunologie)</term>
<term>Lactococcus lactis (génétique)</term>
<term>Muqueuse intestinale (immunologie)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (immunologie)</term>
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<term>Souris de lignée BALB C</term>
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<term>Viral Envelope Proteins</term>
<term>Viral Vaccines</term>
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<term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Vaccines, Synthetic</term>
<term>Viral Envelope Proteins</term>
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<term>Transmissible gastroenteritis virus</term>
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<term>Glycoprotéine de spicule des coronavirus</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
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<front><div type="abstract" xml:lang="en">Lactococcus lactis NZ9000 was selected as an antigen delivery vehicle for mucosal immunization against porcine transmissible gastroenteritis virus (TGEV) infection. An approximately 70 kDa fragment of the N-terminal globular domain of the spike (S) protein (SN protein) from the coronavirus TGEV was used as the transmissible gastroenteritis virus antigen model. Recombinant L. lactis, expressing the SN protein, was constructed with the pNZ8112 plasmid. Expression and localization of the transcribed SN protein from the recombinant LNZ9000- rTGEV-SN were detected via SDS-PAGE, Western blot, and immunofluorescence. BALB/c mice, orally immunized with LNZ9000-rTGEV-SN, produced local mucosal immune responses against TGEV. The induced antibodies demonstrated neutralizing effects on TGEV infection. These data indicated that the recombinant L. lactis could be a valuable tool in the development of future vaccines against TGEV.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
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<tree><noCountry><name sortKey="Li, Yijing" sort="Li, Yijing" uniqKey="Li Y" first="Yijing" last="Li">Yijing Li</name>
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Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein.

Oral immunization of mice with recombinant Lactococcus lactis expressing porcine transmissible gastroenteritis virus spike glycoprotein.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki