[Prokaryotic expression of S2 extracellular domain of SARS coronavirus spike protein and its fusion with Hela cell membrane].
Identifieur interne : 002A07 ( Main/Merge ); précédent : 002A06; suivant : 002A08[Prokaryotic expression of S2 extracellular domain of SARS coronavirus spike protein and its fusion with Hela cell membrane].
Auteurs : Yun Liu [République populaire de Chine] ; Ai-Hua Liu ; Peng Deng ; Xiang-Ling Wu ; Tao Li ; Ya-Wei Liu ; Jia Xu ; Yong JiangSource :
- Nan fang yi ke da xue xue bao = Journal of Southern Medical University [ 1673-4254 ] ; 2009.
Descripteurs français
- KwdFr :
- Cellules HeLa, Escherichia coli (génétique), Escherichia coli (métabolisme), Fusion membranaire (), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (biosynthèse), Glycoprotéines membranaires (génétique), Humains, Protéines de fusion membranaire (biosynthèse), Protéines de fusion membranaire (isolement et purification), Protéines de fusion recombinantes (biosynthèse), Protéines de fusion recombinantes (génétique), Protéines de fusion recombinantes (isolement et purification), Protéines de l'enveloppe virale (biosynthèse), Protéines de l'enveloppe virale (génétique), Protéines à fluorescence verte (biosynthèse), Protéines à fluorescence verte (génétique), Protéines à fluorescence verte (métabolisme), Virus du SRAS (génétique).
- MESH :
- biosynthèse : Glycoprotéines membranaires, Protéines de fusion membranaire, Protéines de fusion recombinantes, Protéines de l'enveloppe virale, Protéines à fluorescence verte.
- génétique : Escherichia coli, Glycoprotéines membranaires, Protéines de fusion recombinantes, Protéines de l'enveloppe virale, Protéines à fluorescence verte, Virus du SRAS.
- isolement et purification : Protéines de fusion membranaire, Protéines de fusion recombinantes.
- métabolisme : Escherichia coli, Protéines à fluorescence verte.
- Cellules HeLa, Fusion membranaire, Glycoprotéine de spicule des coronavirus, Humains.
English descriptors
- KwdEn :
- Escherichia coli (genetics), Escherichia coli (metabolism), Green Fluorescent Proteins (biosynthesis), Green Fluorescent Proteins (genetics), Green Fluorescent Proteins (metabolism), HeLa Cells, Humans, Membrane Fusion (drug effects), Membrane Fusion Proteins (biosynthesis), Membrane Fusion Proteins (isolation & purification), Membrane Glycoproteins (biosynthesis), Membrane Glycoproteins (genetics), Recombinant Fusion Proteins (biosynthesis), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (isolation & purification), SARS Virus (genetics), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (genetics).
- MESH :
- chemical , biosynthesis : Green Fluorescent Proteins, Membrane Fusion Proteins, Membrane Glycoproteins, Recombinant Fusion Proteins, Viral Envelope Proteins.
- drug effects : Membrane Fusion.
- genetics : Escherichia coli, Green Fluorescent Proteins, Membrane Glycoproteins, Recombinant Fusion Proteins, SARS Virus, Viral Envelope Proteins.
- chemical , isolation & purification : Membrane Fusion Proteins, Recombinant Fusion Proteins.
- metabolism : Escherichia coli, Green Fluorescent Proteins.
- HeLa Cells, Humans, Spike Glycoprotein, Coronavirus.
Abstract
To construct the expression plasmid of S2 extracellular domain (S2ED) of SARS-coronavirus (SARS- Cov) spike protein (S protein) and enhanced green fluorescent protein (EGFP) to obtain the fusion protein expressed in prokaryotic cells.
PubMed: 19304506
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pubmed:19304506Le document en format XML
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wicri:level="3"><nlm:affiliation>Department of Pathophysiology and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou, China. sysliuyun@126.com</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathophysiology and Key Laboratory of Proteomics of Guangdong Province, Southern Medical University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Liu, Ai Hua" sort="Liu, Ai Hua" uniqKey="Liu A" first="Ai-Hua" last="Liu">Ai-Hua Liu</name></author><author><name sortKey="Deng, Peng" sort="Deng, Peng" uniqKey="Deng P" first="Peng" last="Deng">Peng Deng</name></author><author><name sortKey="Wu, Xiang Ling" sort="Wu, Xiang Ling" uniqKey="Wu X" first="Xiang-Ling" last="Wu">Xiang-Ling Wu</name></author><author><name sortKey="Li, Tao" sort="Li, Tao" uniqKey="Li T" first="Tao" last="Li">Tao Li</name></author><author><name sortKey="Liu, Ya Wei" sort="Liu, Ya Wei" uniqKey="Liu Y" first="Ya-Wei" last="Liu">Ya-Wei Liu</name></author><author><name sortKey="Xu, Jia" sort="Xu, Jia" uniqKey="Xu J" first="Jia" last="Xu">Jia Xu</name></author><author><name sortKey="Jiang, Yong" sort="Jiang, Yong" uniqKey="Jiang Y" first="Yong" last="Jiang">Yong Jiang</name></author></analytic><series><title level="j">Nan fang yi ke da xue xue bao = Journal of Southern Medical University</title><idno type="ISSN">1673-4254</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Green Fluorescent Proteins (biosynthesis)</term><term>Green Fluorescent Proteins (genetics)</term><term>Green Fluorescent Proteins (metabolism)</term><term>HeLa Cells</term><term>Humans</term><term>Membrane Fusion (drug effects)</term><term>Membrane Fusion Proteins (biosynthesis)</term><term>Membrane Fusion Proteins (isolation & purification)</term><term>Membrane Glycoproteins (biosynthesis)</term><term>Membrane Glycoproteins (genetics)</term><term>Recombinant Fusion Proteins (biosynthesis)</term><term>Recombinant Fusion Proteins (genetics)</term><term>Recombinant Fusion Proteins (isolation & purification)</term><term>SARS Virus (genetics)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cellules HeLa</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Fusion membranaire ()</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (biosynthèse)</term><term>Glycoprotéines membranaires (génétique)</term><term>Humains</term><term>Protéines de fusion membranaire (biosynthèse)</term><term>Protéines de fusion membranaire (isolement et purification)</term><term>Protéines de fusion recombinantes (biosynthèse)</term><term>Protéines de fusion recombinantes (génétique)</term><term>Protéines de fusion recombinantes (isolement et purification)</term><term>Protéines de l'enveloppe virale (biosynthèse)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines à fluorescence verte (biosynthèse)</term><term>Protéines à fluorescence verte (génétique)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Membrane Fusion Proteins</term><term>Membrane Glycoproteins</term><term>Recombinant Fusion Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" 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expression plasmid of S2 extracellular domain (S2ED) of SARS-coronavirus (SARS- Cov) spike protein (S protein) and enhanced green fluorescent protein (EGFP) to obtain the fusion protein expressed in prokaryotic cells.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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