Genomic and serological detection of bat coronavirus from bats in the Philippines
Identifieur interne : 002009 ( Main/Merge ); précédent : 002008; suivant : 002010Genomic and serological detection of bat coronavirus from bats in the Philippines
Auteurs : Shumpei Tsuda [Japon] ; Shumpei Watanabe [Japon] ; Joseph S. Masangkay [Philippines] ; Tetsuya Mizutani [Japon] ; Phillip Alviola [Philippines] ; Naoya Ueda [Japon] ; Koichiro Iha [Japon] ; Satoshi Taniguchi [Japon] ; Hikaru Fujii [Japon] ; Kentaro Kato [Japon] ; Taisuke Horimoto [Japon] ; Shigeru Kyuwa [Japon] ; Yasuhiro Yoshikawa [Japon] ; Hiroomi Akashi [Japon]Source :
- Archives of virology [ 0304-8608 ] ; 2012.
Descripteurs français
- Pascal (Inist)
- Wicri :
- geographic : Philippines.
English descriptors
- KwdEn :
Abstract
Bat coronavirus (BtCoV) is assumed to be a progenitor of severe acute respiratory syndrome (SARS)-related coronaviruses. To explore the distribution of BtCoVs in the Philippines, we collected 179 bats and detected viral RNA from intestinal or fecal samples by RT-PCR. The overall prevalence of BtCoVs among bats was 29.6 %. Phylogenetic analysis of the partial RNA-dependent RNA polymerase gene suggested that one of the detected BtCoVs was a novel alphacoronavirus, while the others belonged to the genus Betacoronavirus. Western blotting revealed that 66.5 % of bat sera had antibodies to BtCoV. These surveys suggested the endemic presence of BtCoVs in the Philippines.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Genomic and serological detection of bat coronavirus from bats in the Philippines</title><author><name sortKey="Tsuda, Shumpei" sort="Tsuda, Shumpei" uniqKey="Tsuda S" first="Shumpei" last="Tsuda">Shumpei Tsuda</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences. The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Watanabe, Shumpei" sort="Watanabe, Shumpei" uniqKey="Watanabe S" first="Shumpei" last="Watanabe">Shumpei Watanabe</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences. The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Masangkay, Joseph S" sort="Masangkay, Joseph S" uniqKey="Masangkay J" first="Joseph S." last="Masangkay">Joseph S. Masangkay</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>College of Veterinary Medicine, University of the Philippines Los Baños, College</s1><s2>4031 Laguna</s2><s3>PHL</s3><sZ>3 aut.</sZ></inist:fA14><country>Philippines</country><wicri:noRegion>4031 Laguna</wicri:noRegion></affiliation></author><author><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Alviola, Phillip" sort="Alviola, Phillip" uniqKey="Alviola P" first="Phillip" last="Alviola">Phillip Alviola</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Museum of National History, University of the Philippines Los Banos, College</s1><s2>4031 Laguna</s2><s3>PHL</s3><sZ>5 aut.</sZ></inist:fA14><country>Philippines</country><wicri:noRegion>4031 Laguna</wicri:noRegion></affiliation></author><author><name sortKey="Ueda, Naoya" sort="Ueda, Naoya" uniqKey="Ueda N" first="Naoya" last="Ueda">Naoya Ueda</name><affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>6 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Iha, Koichiro" sort="Iha, Koichiro" uniqKey="Iha K" first="Koichiro" last="Iha">Koichiro Iha</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Taniguchi, Satoshi" sort="Taniguchi, Satoshi" uniqKey="Taniguchi S" first="Satoshi" last="Taniguchi">Satoshi Taniguchi</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Fujii, Hikaru" sort="Fujii, Hikaru" uniqKey="Fujii H" first="Hikaru" last="Fujii">Hikaru Fujii</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences. The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kato, Kentaro" sort="Kato, Kentaro" uniqKey="Kato K" first="Kentaro" last="Kato">Kentaro Kato</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences. The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Horimoto, Taisuke" sort="Horimoto, Taisuke" uniqKey="Horimoto T" first="Taisuke" last="Horimoto">Taisuke Horimoto</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences. The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kyuwa, Shigeru" sort="Kyuwa, Shigeru" uniqKey="Kyuwa S" first="Shigeru" last="Kyuwa">Shigeru Kyuwa</name><affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>6 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yoshikawa, Yasuhiro" sort="Yoshikawa, Yasuhiro" uniqKey="Yoshikawa Y" first="Yasuhiro" last="Yoshikawa">Yasuhiro Yoshikawa</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Laboratory of Zoonosis, School of Veterinary Medicine, Kitasato University, Higashi 23-bancho</s1><s2>Towada, Aomori 034-8628</s2><s3>JPN</s3><sZ>13 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Towada, Aomori 034-8628</wicri:noRegion></affiliation></author><author><name sortKey="Akashi, Hiroomi" sort="Akashi, Hiroomi" uniqKey="Akashi H" first="Hiroomi" last="Akashi">Hiroomi Akashi</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences. The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">13-0026617</idno><date when="2012">2012</date><idno type="stanalyst">PASCAL 13-0026617 INIST</idno><idno type="RBID">Pascal:13-0026617</idno><idno type="wicri:Area/PascalFrancis/Corpus">000030</idno><idno type="wicri:Area/PascalFrancis/Curation">000957</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000058</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000058</idno><idno type="wicri:doubleKey">0304-8608:2012:Tsuda S:genomic:and:serological</idno><idno type="wicri:Area/Main/Merge">002009</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Genomic and serological detection of bat coronavirus from bats in the Philippines</title><author><name sortKey="Tsuda, Shumpei" sort="Tsuda, Shumpei" uniqKey="Tsuda S" first="Shumpei" last="Tsuda">Shumpei Tsuda</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences. The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Watanabe, Shumpei" sort="Watanabe, Shumpei" uniqKey="Watanabe S" first="Shumpei" last="Watanabe">Shumpei Watanabe</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences. The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Masangkay, Joseph S" sort="Masangkay, Joseph S" uniqKey="Masangkay J" first="Joseph S." last="Masangkay">Joseph S. Masangkay</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>College of Veterinary Medicine, University of the Philippines Los Baños, College</s1><s2>4031 Laguna</s2><s3>PHL</s3><sZ>3 aut.</sZ></inist:fA14><country>Philippines</country><wicri:noRegion>4031 Laguna</wicri:noRegion></affiliation></author><author><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Alviola, Phillip" sort="Alviola, Phillip" uniqKey="Alviola P" first="Phillip" last="Alviola">Phillip Alviola</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Museum of National History, University of the Philippines Los Banos, College</s1><s2>4031 Laguna</s2><s3>PHL</s3><sZ>5 aut.</sZ></inist:fA14><country>Philippines</country><wicri:noRegion>4031 Laguna</wicri:noRegion></affiliation></author><author><name sortKey="Ueda, Naoya" sort="Ueda, Naoya" uniqKey="Ueda N" first="Naoya" last="Ueda">Naoya Ueda</name><affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>6 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Iha, Koichiro" sort="Iha, Koichiro" uniqKey="Iha K" first="Koichiro" last="Iha">Koichiro Iha</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Taniguchi, Satoshi" sort="Taniguchi, Satoshi" uniqKey="Taniguchi S" first="Satoshi" last="Taniguchi">Satoshi Taniguchi</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Virology 1, National Institute of Infectious Diseases, Gakuen 4-7-1</s1><s2>Musashimurayama, Tokyo 208-0011</s2><s3>JPN</s3><sZ>4 aut.</sZ><sZ>7 aut.</sZ><sZ>8 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Fujii, Hikaru" sort="Fujii, Hikaru" uniqKey="Fujii H" first="Hikaru" last="Fujii">Hikaru Fujii</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences. The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kato, Kentaro" sort="Kato, Kentaro" uniqKey="Kato K" first="Kentaro" last="Kato">Kentaro Kato</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences. The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Horimoto, Taisuke" sort="Horimoto, Taisuke" uniqKey="Horimoto T" first="Taisuke" last="Horimoto">Taisuke Horimoto</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences. The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Kyuwa, Shigeru" sort="Kyuwa, Shigeru" uniqKey="Kyuwa S" first="Shigeru" last="Kyuwa">Shigeru Kyuwa</name><affiliation wicri:level="4"><inist:fA14 i1="05"><s1>Department of Biomedical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>6 aut.</sZ><sZ>12 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Yoshikawa, Yasuhiro" sort="Yoshikawa, Yasuhiro" uniqKey="Yoshikawa Y" first="Yasuhiro" last="Yoshikawa">Yasuhiro Yoshikawa</name><affiliation wicri:level="1"><inist:fA14 i1="06"><s1>Laboratory of Zoonosis, School of Veterinary Medicine, Kitasato University, Higashi 23-bancho</s1><s2>Towada, Aomori 034-8628</s2><s3>JPN</s3><sZ>13 aut.</sZ></inist:fA14><country>Japon</country><wicri:noRegion>Towada, Aomori 034-8628</wicri:noRegion></affiliation></author><author><name sortKey="Akashi, Hiroomi" sort="Akashi, Hiroomi" uniqKey="Akashi H" first="Hiroomi" last="Akashi">Hiroomi Akashi</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Department of Veterinary Microbiology, Graduate School of Agricultural and Life Sciences. The University of Tokyo, 1-1-1 Yayoi</s1><s2>Bunkyo-ku, Tokyo 113-8657</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>9 aut.</sZ><sZ>10 aut.</sZ><sZ>11 aut.</sZ><sZ>14 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName><orgName type="university">Université de Tokyo</orgName><placeName><settlement type="city">Tokyo</settlement><region type="province">Région de Kantō</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Archives of virology</title><title level="j" type="abbreviated">Arch. virol.</title><idno type="ISSN">0304-8608</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Archives of virology</title><title level="j" type="abbreviated">Arch. virol.</title><idno type="ISSN">0304-8608</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term><term>Detection</term><term>Genomics</term><term>Philippines</term><term>Serology</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Génomique</term><term>Sérologie</term><term>Détection</term><term>Philippines</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Philippines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bat coronavirus (BtCoV) is assumed to be a progenitor of severe acute respiratory syndrome (SARS)-related coronaviruses. To explore the distribution of BtCoVs in the Philippines, we collected 179 bats and detected viral RNA from intestinal or fecal samples by RT-PCR. The overall prevalence of BtCoVs among bats was 29.6 %. Phylogenetic analysis of the partial RNA-dependent RNA polymerase gene suggested that one of the detected BtCoVs was a novel alphacoronavirus, while the others belonged to the genus Betacoronavirus. Western blotting revealed that 66.5 % of bat sera had antibodies to BtCoV. These surveys suggested the endemic presence of BtCoVs in the Philippines.</div></front></TEI><affiliations><list><country><li>Japon</li><li>Philippines</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement><orgName><li>Université de Tokyo</li></orgName></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Tsuda, Shumpei" sort="Tsuda, Shumpei" uniqKey="Tsuda S" first="Shumpei" last="Tsuda">Shumpei Tsuda</name></region><name sortKey="Akashi, Hiroomi" sort="Akashi, Hiroomi" uniqKey="Akashi H" first="Hiroomi" last="Akashi">Hiroomi Akashi</name><name sortKey="Fujii, Hikaru" sort="Fujii, Hikaru" uniqKey="Fujii H" first="Hikaru" last="Fujii">Hikaru Fujii</name><name sortKey="Horimoto, Taisuke" sort="Horimoto, Taisuke" uniqKey="Horimoto T" first="Taisuke" last="Horimoto">Taisuke Horimoto</name><name sortKey="Iha, Koichiro" sort="Iha, Koichiro" uniqKey="Iha K" first="Koichiro" last="Iha">Koichiro Iha</name><name sortKey="Kato, Kentaro" sort="Kato, Kentaro" uniqKey="Kato K" first="Kentaro" last="Kato">Kentaro Kato</name><name sortKey="Kyuwa, Shigeru" sort="Kyuwa, Shigeru" uniqKey="Kyuwa S" first="Shigeru" last="Kyuwa">Shigeru Kyuwa</name><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><name sortKey="Taniguchi, Satoshi" sort="Taniguchi, Satoshi" uniqKey="Taniguchi S" first="Satoshi" last="Taniguchi">Satoshi Taniguchi</name><name sortKey="Ueda, Naoya" sort="Ueda, Naoya" uniqKey="Ueda N" first="Naoya" last="Ueda">Naoya Ueda</name><name sortKey="Watanabe, Shumpei" sort="Watanabe, Shumpei" uniqKey="Watanabe S" first="Shumpei" last="Watanabe">Shumpei Watanabe</name><name sortKey="Yoshikawa, Yasuhiro" sort="Yoshikawa, Yasuhiro" uniqKey="Yoshikawa Y" first="Yasuhiro" last="Yoshikawa">Yasuhiro Yoshikawa</name></country><country name="Philippines"><noRegion><name sortKey="Masangkay, Joseph S" sort="Masangkay, Joseph S" uniqKey="Masangkay J" first="Joseph S." last="Masangkay">Joseph S. Masangkay</name></noRegion><name sortKey="Alviola, Phillip" sort="Alviola, Phillip" uniqKey="Alviola P" first="Phillip" last="Alviola">Phillip Alviola</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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