SrasV1, Main, Merge, bibRecord, 000D68

Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA

Identifieur interne : 000D68 ( Main/Merge ); précédent : 000D67; suivant : 000D69

Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA

Auteurs : François Ferron [France] ; Lorenzo Subissi [France] ; Ana Theresa Silveira De Morais [France] ; Nhung Thi Tuyet Le [France] ; Marion Sevajol [France] ; Laure Gluais [France] ; Etienne Decroly [France] ; Clemens Vonrhein ; Gérard Bricogne ; Bruno Canard [France] ; Isabelle Imbert [France]

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2017.

RBID : PMC:5777078

Descripteurs français

English descriptors

KwdEn :
- Antiviral Agents (metabolism), Antiviral Agents (pharmacology), Coronavirus (drug effects), Coronavirus (genetics), Coronavirus (metabolism), Crystallography, X-Ray, Exoribonucleases (chemistry), Exoribonucleases (genetics), Exoribonucleases (metabolism), Humans, Methyltransferases (chemistry), Methyltransferases (genetics), Methyltransferases (metabolism), Models, Molecular, Protein Binding, Protein Domains, RNA, Viral (genetics), RNA, Viral (metabolism), Ribavirin (metabolism), Ribavirin (pharmacology), Severe Acute Respiratory Syndrome (virology), Viral Nonstructural Proteins (chemistry), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism), Virus Replication.
MESH :
- chemical , chemistry : Exoribonucleases, Methyltransferases, Viral Nonstructural Proteins.
- chemical , genetics : Exoribonucleases, Methyltransferases, RNA, Viral, Viral Nonstructural Proteins.
- chemical , metabolism : Antiviral Agents, Exoribonucleases, Methyltransferases, RNA, Viral, Ribavirin, Viral Nonstructural Proteins.
- chemical , pharmacology : Antiviral Agents, Ribavirin.
- drug effects : Coronavirus.
- genetics : Coronavirus.
- metabolism : Coronavirus.
- virology : Severe Acute Respiratory Syndrome.
- Crystallography, X-Ray, Humans, Models, Molecular, Protein Binding, Protein Domains, Virus Replication.
mix :
- RNA virus, fold evolution, proofreading, ribavirin, virus replication.

Abstract

Significance

Emerging coronaviruses (CoVs; severe acute respiratory syndrome-CoV and Middle East respiratory syndrome-CoV) pose serious health threats globally, with no specific antiviral treatments available. These viruses are able to faithfully synthesize their large genomic RNA. We report, however, that their main RNA polymerase, nsp12, is not accurate. To achieve accuracy, CoVs have acquired nsp14, a bifunctional enzyme able to methylate the viral RNA cap [methyltransferase (MTase)] and excise erroneous mutagenic nucleotides inserted by nsp12. Strikingly, ribavirin can be excised from the viral genome, thus showing no antiviral activity. The crystal structure of nsp14 shows that it is unique, having been replaced by other MTase types during evolution. This unprecedented RNA correction machinery has allowed RNA genome size expansion, but also provided potential nucleoside drug resistance to these deadly pathogens.

Url:

DOI: 10.1073/pnas.1718806115
PubMed: 29279395
PubMed Central: 5777078

Links to Exploration step

PMC:5777078

Le document en format XML

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<front><div type="abstract" xml:lang="en"><title>Significance</title>
<p>Emerging coronaviruses (CoVs; severe acute respiratory syndrome-CoV and Middle East respiratory syndrome-CoV) pose serious health threats globally, with no specific antiviral treatments available. These viruses are able to faithfully synthesize their large genomic RNA. We report, however, that their main RNA polymerase, nsp12, is not accurate. To achieve accuracy, CoVs have acquired nsp14, a bifunctional enzyme able to methylate the viral RNA cap [methyltransferase (MTase)] and excise erroneous mutagenic nucleotides inserted by nsp12. Strikingly, ribavirin can be excised from the viral genome, thus showing no antiviral activity. The crystal structure of nsp14 shows that it is unique, having been replaced by other MTase types during evolution. This unprecedented RNA correction machinery has allowed RNA genome size expansion, but also provided potential nucleoside drug resistance to these deadly pathogens.</p>
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<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Drake, Jw" uniqKey="Drake J">JW Drake</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Belshaw, R" uniqKey="Belshaw R">R Belshaw</name>
</author>
<author><name sortKey="Gardner, A" uniqKey="Gardner A">A Gardner</name>
</author>
<author><name sortKey="Rambaut, A" uniqKey="Rambaut A">A Rambaut</name>
</author>
<author><name sortKey="Pybus, Og" uniqKey="Pybus O">OG Pybus</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sanjuan, R" uniqKey="Sanjuan R">R Sanjuán</name>
</author>
<author><name sortKey="Nebot, Mr" uniqKey="Nebot M">MR Nebot</name>
</author>
<author><name sortKey="Chirico, N" uniqKey="Chirico N">N Chirico</name>
</author>
<author><name sortKey="Mansky, Lm" uniqKey="Mansky L">LM Mansky</name>
</author>
<author><name sortKey="Belshaw, R" uniqKey="Belshaw R">R Belshaw</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sidwell, Rw" uniqKey="Sidwell R">RW Sidwell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Parker, Wb" uniqKey="Parker W">WB Parker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crotty, S" uniqKey="Crotty S">S Crotty</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mchutchison, Jg" uniqKey="Mchutchison J">JG McHutchison</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hall, Cb" uniqKey="Hall C">CB Hall</name>
</author>
<author><name sortKey="Walsh, Ee" uniqKey="Walsh E">EE Walsh</name>
</author>
<author><name sortKey="Hruska, Jf" uniqKey="Hruska J">JF Hruska</name>
</author>
<author><name sortKey="Betts, Rf" uniqKey="Betts R">RF Betts</name>
</author>
<author><name sortKey="Hall, Wj" uniqKey="Hall W">WJ Hall</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mccormick, Jb" uniqKey="Mccormick J">JB McCormick</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stockman, Lj" uniqKey="Stockman L">LJ Stockman</name>
</author>
<author><name sortKey="Bellamy, R" uniqKey="Bellamy R">R Bellamy</name>
</author>
<author><name sortKey="Garner, P" uniqKey="Garner P">P Garner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Al Tawfiq, Ja" uniqKey="Al Tawfiq J">JA Al-Tawfiq</name>
</author>
<author><name sortKey="Memish, Za" uniqKey="Memish Z">ZA Memish</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stadler, K" uniqKey="Stadler K">K Stadler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zaki, Am" uniqKey="Zaki A">AM Zaki</name>
</author>
<author><name sortKey="Van Boheemen, S" uniqKey="Van Boheemen S">S van Boheemen</name>
</author>
<author><name sortKey="Bestebroer, Tm" uniqKey="Bestebroer T">TM Bestebroer</name>
</author>
<author><name sortKey="Osterhaus, Adme" uniqKey="Osterhaus A">ADME Osterhaus</name>
</author>
<author><name sortKey="Fouchier, Ram" uniqKey="Fouchier R">RAM Fouchier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Perlman, S" uniqKey="Perlman S">S Perlman</name>
</author>
<author><name sortKey="Netland, J" uniqKey="Netland J">J Netland</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Subissi, L" uniqKey="Subissi L">L Subissi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bouvet, M" uniqKey="Bouvet M">M Bouvet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ricagno, S" uniqKey="Ricagno S">S Ricagno</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kang, H" uniqKey="Kang H">H Kang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kindler, E" uniqKey="Kindler E">E Kindler</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Minskaia, E" uniqKey="Minskaia E">E Minskaia</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Martinez Sobrido, L" uniqKey="Martinez Sobrido L">L Martínez-Sobrido</name>
</author>
<author><name sortKey="Zu Iga, Ei" uniqKey="Zu Iga E">EI Zúñiga</name>
</author>
<author><name sortKey="Rosario, D" uniqKey="Rosario D">D Rosario</name>
</author>
<author><name sortKey="Garcia Sastre, A" uniqKey="Garcia Sastre A">A García-Sastre</name>
</author>
<author><name sortKey="De La Torre, Jc" uniqKey="De La Torre J">JC de la Torre</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Qi, X" uniqKey="Qi X">X Qi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gorbalenya, Ae" uniqKey="Gorbalenya A">AE Gorbalenya</name>
</author>
<author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name>
</author>
<author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J Ziebuhr</name>
</author>
<author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lauber, C" uniqKey="Lauber C">C Lauber</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Eckerle, Ld" uniqKey="Eckerle L">LD Eckerle</name>
</author>
<author><name sortKey="Lu, X" uniqKey="Lu X">X Lu</name>
</author>
<author><name sortKey="Sperry, Sm" uniqKey="Sperry S">SM Sperry</name>
</author>
<author><name sortKey="Choi, L" uniqKey="Choi L">L Choi</name>
</author>
<author><name sortKey="Denison, Mr" uniqKey="Denison M">MR Denison</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Eckerle, Ld" uniqKey="Eckerle L">LD Eckerle</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Smith, Ec" uniqKey="Smith E">EC Smith</name>
</author>
<author><name sortKey="Blanc, H" uniqKey="Blanc H">H Blanc</name>
</author>
<author><name sortKey="Surdel, Mc" uniqKey="Surdel M">MC Surdel</name>
</author>
<author><name sortKey="Vignuzzi, M" uniqKey="Vignuzzi M">M Vignuzzi</name>
</author>
<author><name sortKey="Denison, Mr" uniqKey="Denison M">MR Denison</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bouvet, M" uniqKey="Bouvet M">M Bouvet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ma, Y" uniqKey="Ma Y">Y Ma</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Joseph, Js" uniqKey="Joseph J">JS Joseph</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Su, D" uniqKey="Su D">D Su</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Decroly, E" uniqKey="Decroly E">E Decroly</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Derbyshire, V" uniqKey="Derbyshire V">V Derbyshire</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cheng, Y" uniqKey="Cheng Y">Y Cheng</name>
</author>
<author><name sortKey="Patel, Dj" uniqKey="Patel D">DJ Patel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zuo, Y" uniqKey="Zuo Y">Y Zuo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rauscher, S" uniqKey="Rauscher S">S Rauscher</name>
</author>
<author><name sortKey="Pomes, R" uniqKey="Pomes R">R Pomès</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, Y" uniqKey="Chen Y">Y Chen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Byszewska, M" uniqKey="Byszewska M">M Byszewska</name>
</author>
<author><name sortKey=" Mieta Ski, M" uniqKey=" Mieta Ski M">M Śmietański</name>
</author>
<author><name sortKey="Purta, E" uniqKey="Purta E">E Purta</name>
</author>
<author><name sortKey="Bujnicki, Jm" uniqKey="Bujnicki J">JM Bujnicki</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rao, St" uniqKey="Rao S">ST Rao</name>
</author>
<author><name sortKey="Rossmann, Mg" uniqKey="Rossmann M">MG Rossmann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hanukoglu, I" uniqKey="Hanukoglu I">I Hanukoglu</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Imbert, I" uniqKey="Imbert I">I Imbert</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bouvet, M" uniqKey="Bouvet M">M Bouvet</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jin, Z" uniqKey="Jin Z">Z Jin</name>
</author>
<author><name sortKey="Deval, J" uniqKey="Deval J">J Deval</name>
</author>
<author><name sortKey="Johnson, Ka" uniqKey="Johnson K">KA Johnson</name>
</author>
<author><name sortKey="Swinney, Dc" uniqKey="Swinney D">DC Swinney</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Drake, Jw" uniqKey="Drake J">JW Drake</name>
</author>
<author><name sortKey="Charlesworth, B" uniqKey="Charlesworth B">B Charlesworth</name>
</author>
<author><name sortKey="Charlesworth, D" uniqKey="Charlesworth D">D Charlesworth</name>
</author>
<author><name sortKey="Crow, Jf" uniqKey="Crow J">JF Crow</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name>
</author>
<author><name sortKey="Decroly, E" uniqKey="Decroly E">E Decroly</name>
</author>
<author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J Ziebuhr</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Becares, M" uniqKey="Becares M">M Becares</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Warren, Tk" uniqKey="Warren T">TK Warren</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sheahan, Tp" uniqKey="Sheahan T">TP Sheahan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vonrhein, C" uniqKey="Vonrhein C">C Vonrhein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vonrhein, C" uniqKey="Vonrhein C">C Vonrhein</name>
</author>
<author><name sortKey="Blanc, E" uniqKey="Blanc E">E Blanc</name>
</author>
<author><name sortKey="Roversi, P" uniqKey="Roversi P">P Roversi</name>
</author>
<author><name sortKey="Bricogne, G" uniqKey="Bricogne G">G Bricogne</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cowtan, K" uniqKey="Cowtan K">K Cowtan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Emsley, P" uniqKey="Emsley P">P Emsley</name>
</author>
<author><name sortKey="Cowtan, K" uniqKey="Cowtan K">K Cowtan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Blanc, E" uniqKey="Blanc E">E Blanc</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Dimaio, F" uniqKey="Dimaio F">F DiMaio</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chen, Vb" uniqKey="Chen V">VB Chen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pettersen, Ef" uniqKey="Pettersen E">EF Pettersen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bugl, H" uniqKey="Bugl H">H Bügl</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Potisopon, S" uniqKey="Potisopon S">S Potisopon</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
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<double doi="10.1073/pnas.1718806115"><HAL><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA</title>
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<author><name sortKey="Decroly, Etienne" sort="Decroly, Etienne" uniqKey="Decroly E" first="Etienne" last="Decroly">Etienne Decroly</name>
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</author>
<author><name sortKey="Vonrhein, Clemens" sort="Vonrhein, Clemens" uniqKey="Vonrhein C" first="Clemens" last="Vonrhein">Clemens Vonrhein</name>
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<author><name sortKey="Bricogne, Gerard" sort="Bricogne, Gerard" uniqKey="Bricogne G" first="Gérard" last="Bricogne">Gérard Bricogne</name>
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<country>Royaume-Uni</country>
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<author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name>
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          <orgName type="acronym">INRA</orgName>
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<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Imbert, Isabelle" sort="Imbert, Isabelle" uniqKey="Imbert I" first="Isabelle" last="Imbert">Isabelle Imbert</name>
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</titleStmt>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA</title>
<author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="Francois" last="Ferron">Francois Ferron</name>
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          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
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          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
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          <orgName>Aix Marseille Université</orgName>
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<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
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<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Subissi, Lorenzo" sort="Subissi, Lorenzo" uniqKey="Subissi L" first="Lorenzo" last="Subissi">Lorenzo Subissi</name>
</author>
<author><name sortKey="Silveira De Morais, Ana Theresa" sort="Silveira De Morais, Ana Theresa" uniqKey="Silveira De Morais A" first="Ana Theresa" last="Silveira De Morais">Ana Theresa Silveira De Morais</name>
</author>
<author><name sortKey="Le, Nhung Thi Tuyet" sort="Le, Nhung Thi Tuyet" uniqKey="Le N" first="Nhung Thi Tuyet" last="Le">Nhung Thi Tuyet Le</name>
</author>
<author><name sortKey="Sevajol, Marion" sort="Sevajol, Marion" uniqKey="Sevajol M" first="Marion" last="Sevajol">Marion Sevajol</name>
</author>
<author><name sortKey="Gluais, Laure" sort="Gluais, Laure" uniqKey="Gluais L" first="Laure" last="Gluais">Laure Gluais</name>
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          <orgName type="acronym">AMU ODONTO</orgName>
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            </address>
            <ref type="url">http://odontologie.univ-amu.fr/</ref>
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<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Decroly, Etienne" sort="Decroly, Etienne" uniqKey="Decroly E" first="Etienne" last="Decroly">Etienne Decroly</name>
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          <idno type="RNSR">201220260Z</idno>
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          <orgName type="acronym">AFMB</orgName>
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          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
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          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
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            </address>
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<tutelle name="UMR7257" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID">          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
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<tutelle name="UMR7257" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID">          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
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            </address>
            <ref type="url">http://www.cnrs.fr/</ref>
          </desc>
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</hal:affiliation>
<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Vonrhein, Clemens" sort="Vonrhein, Clemens" uniqKey="Vonrhein C" first="Clemens" last="Vonrhein">Clemens Vonrhein</name>
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            </address>
          </desc>
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</tutelles>
</hal:affiliation>
<country>Royaume-Uni</country>
</affiliation>
</author>
<author><name sortKey="Bricogne, Gerard" sort="Bricogne, Gerard" uniqKey="Bricogne G" first="Gérard" last="Bricogne">Gérard Bricogne</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-135565" status="INCOMING">          <orgName>Global Phasing Ltd</orgName>
          <desc>            <address>              <addrLine>Sheraton House, Castle Park, Cambridge CB3 0AX</addrLine>
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        <tutelles><tutelle active="#struct-325932" type="direct"><org type="institution" xml:id="struct-325932" status="INCOMING">          <orgName>Global Phasing</orgName>
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            </address>
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</tutelles>
</hal:affiliation>
<country>Royaume-Uni</country>
</affiliation>
</author>
<author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name>
<affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-199349" status="OLD">          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>            <address>              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy13288 Marseille Cedex 09</addrLine>
              <country key="FR"></country>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
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          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
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            </address>
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          <idno type="ISNI">0000 0001 2176 4817</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>            <address>              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"></country>
            </address>
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          <orgName type="acronym">CNRS</orgName>
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<country>France</country>
<placeName><settlement type="city">Marseille</settlement>
<region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region>
</placeName>
<orgName type="university">Université d'Aix-Marseille</orgName>
</affiliation>
</author>
<author><name sortKey="Imbert, Isabelle" sort="Imbert, Isabelle" uniqKey="Imbert I" first="Isabelle" last="Imbert">Isabelle Imbert</name>
</author>
</analytic>
<idno type="DOI">10.1073/pnas.1718806115</idno>
<series><title level="j">Proceedings of the National Academy of Sciences of the United States of America </title>
<idno type="ISSN">0027-8424</idno>
<imprint><date type="datePub">2018-01-09</date>
</imprint>
</series>
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<profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>RNA virus</term>
<term>fold evolution</term>
<term>proofreading</term>
<term>ribavirin</term>
<term>virus replication</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">              <p>Coronaviruses (CoVs) stand out among RNA viruses because of their unusually large genomes (∼30 kb) associated with low mutation rates. CoVs code for nsp14, a bifunctional enzyme carrying RNA cap guanine N7-methyltransferase (MTase) and 3'-5' exoribonuclease (ExoN) activities. ExoN excises nucleotide mismatches at the RNA 3'-end in vitro, and its inactivation in vivo jeopardizes viral genetic stability. Here, we demonstrate for severe acute respiratory syndrome (SARS)-CoV an RNA synthesis and proofreading pathway through association of nsp14 with the low-fidelity nsp12 viral RNA polymerase. Through this pathway, the antiviral compound ribavirin 5'-monophosphate is significantly incorporated but also readily excised from RNA, which may explain its limited efficacy in vivo. The crystal structure at 3.38 Å resolution of SARS-CoV nsp14 in complex with its cofactor nsp10 adds to the uniqueness of CoVs among RNA viruses: The MTase domain presents a new fold that differs sharply from the canonical Rossmann fold.</p>
            </div>
</front>
</TEI>
</HAL>
<PMC><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA</title>
<author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Subissi, Lorenzo" sort="Subissi, Lorenzo" uniqKey="Subissi L" first="Lorenzo" last="Subissi">Lorenzo Subissi</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Silveira De Morais, Ana Theresa" sort="Silveira De Morais, Ana Theresa" uniqKey="Silveira De Morais A" first="Ana Theresa" last="Silveira De Morais">Ana Theresa Silveira De Morais</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Le, Nhung Thi Tuyet" sort="Le, Nhung Thi Tuyet" uniqKey="Le N" first="Nhung Thi Tuyet" last="Le">Nhung Thi Tuyet Le</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Sevajol, Marion" sort="Sevajol, Marion" uniqKey="Sevajol M" first="Marion" last="Sevajol">Marion Sevajol</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Gluais, Laure" sort="Gluais, Laure" uniqKey="Gluais L" first="Laure" last="Gluais">Laure Gluais</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Decroly, Etienne" sort="Decroly, Etienne" uniqKey="Decroly E" first="Etienne" last="Decroly">Etienne Decroly</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Vonrhein, Clemens" sort="Vonrhein, Clemens" uniqKey="Vonrhein C" first="Clemens" last="Vonrhein">Clemens Vonrhein</name>
<affiliation><nlm:aff id="aff2"><institution>Global Phasing Ltd.</institution>
, Cambridge CB3 0AX,<country>England</country>
</nlm:aff>
<wicri:noCountry code="nlm country">England</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Bricogne, Gerard" sort="Bricogne, Gerard" uniqKey="Bricogne G" first="Gérard" last="Bricogne">Gérard Bricogne</name>
<affiliation><nlm:aff id="aff2"><institution>Global Phasing Ltd.</institution>
, Cambridge CB3 0AX,<country>England</country>
</nlm:aff>
<wicri:noCountry code="nlm country">England</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Imbert, Isabelle" sort="Imbert, Isabelle" uniqKey="Imbert I" first="Isabelle" last="Imbert">Isabelle Imbert</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA</title>
<author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Subissi, Lorenzo" sort="Subissi, Lorenzo" uniqKey="Subissi L" first="Lorenzo" last="Subissi">Lorenzo Subissi</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Silveira De Morais, Ana Theresa" sort="Silveira De Morais, Ana Theresa" uniqKey="Silveira De Morais A" first="Ana Theresa" last="Silveira De Morais">Ana Theresa Silveira De Morais</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Le, Nhung Thi Tuyet" sort="Le, Nhung Thi Tuyet" uniqKey="Le N" first="Nhung Thi Tuyet" last="Le">Nhung Thi Tuyet Le</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Sevajol, Marion" sort="Sevajol, Marion" uniqKey="Sevajol M" first="Marion" last="Sevajol">Marion Sevajol</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Gluais, Laure" sort="Gluais, Laure" uniqKey="Gluais L" first="Laure" last="Gluais">Laure Gluais</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Decroly, Etienne" sort="Decroly, Etienne" uniqKey="Decroly E" first="Etienne" last="Decroly">Etienne Decroly</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Vonrhein, Clemens" sort="Vonrhein, Clemens" uniqKey="Vonrhein C" first="Clemens" last="Vonrhein">Clemens Vonrhein</name>
<affiliation><nlm:aff id="aff2"><institution>Global Phasing Ltd.</institution>
, Cambridge CB3 0AX,<country>England</country>
</nlm:aff>
<wicri:noCountry code="nlm country">England</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Bricogne, Gerard" sort="Bricogne, Gerard" uniqKey="Bricogne G" first="Gérard" last="Bricogne">Gérard Bricogne</name>
<affiliation><nlm:aff id="aff2"><institution>Global Phasing Ltd.</institution>
, Cambridge CB3 0AX,<country>England</country>
</nlm:aff>
<wicri:noCountry code="nlm country">England</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Imbert, Isabelle" sort="Imbert, Isabelle" uniqKey="Imbert I" first="Isabelle" last="Imbert">Isabelle Imbert</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Centre National de la Recherche Scientifique,<institution>Aix-Marseille Université</institution>
, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title>
<idno type="ISSN">0027-8424</idno>
<idno type="eISSN">1091-6490</idno>
<imprint><date when="2017">2017</date>
</imprint>
</series>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antiviral Agents (metabolism)</term>
<term>Antiviral Agents (pharmacology)</term>
<term>Coronavirus (drug effects)</term>
<term>Coronavirus (genetics)</term>
<term>Coronavirus (metabolism)</term>
<term>Crystallography, X-Ray</term>
<term>Exoribonucleases (chemistry)</term>
<term>Exoribonucleases (genetics)</term>
<term>Exoribonucleases (metabolism)</term>
<term>Humans</term>
<term>Methyltransferases (chemistry)</term>
<term>Methyltransferases (genetics)</term>
<term>Methyltransferases (metabolism)</term>
<term>Models, Molecular</term>
<term>Protein Binding</term>
<term>Protein Domains</term>
<term>RNA, Viral (genetics)</term>
<term>RNA, Viral (metabolism)</term>
<term>Ribavirin (metabolism)</term>
<term>Ribavirin (pharmacology)</term>
<term>Severe Acute Respiratory Syndrome (virology)</term>
<term>Viral Nonstructural Proteins (chemistry)</term>
<term>Viral Nonstructural Proteins (genetics)</term>
<term>Viral Nonstructural Proteins (metabolism)</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term>
<term>ARN viral (métabolisme)</term>
<term>Antiviraux (métabolisme)</term>
<term>Antiviraux (pharmacologie)</term>
<term>Coronavirus ()</term>
<term>Coronavirus (génétique)</term>
<term>Coronavirus (métabolisme)</term>
<term>Cristallographie aux rayons X</term>
<term>Domaines protéiques</term>
<term>Exoribonucleases ()</term>
<term>Exoribonucleases (génétique)</term>
<term>Exoribonucleases (métabolisme)</term>
<term>Humains</term>
<term>Liaison aux protéines</term>
<term>Methyltransferases ()</term>
<term>Methyltransferases (génétique)</term>
<term>Methyltransferases (métabolisme)</term>
<term>Modèles moléculaires</term>
<term>Protéines virales non structurales ()</term>
<term>Protéines virales non structurales (génétique)</term>
<term>Protéines virales non structurales (métabolisme)</term>
<term>Ribavirine (métabolisme)</term>
<term>Ribavirine (pharmacologie)</term>
<term>Réplication virale</term>
<term>Syndrome respiratoire aigu sévère (virologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Exoribonucleases</term>
<term>Methyltransferases</term>
<term>Viral Nonstructural Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Exoribonucleases</term>
<term>Methyltransferases</term>
<term>RNA, Viral</term>
<term>Viral Nonstructural Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antiviral Agents</term>
<term>Exoribonucleases</term>
<term>Methyltransferases</term>
<term>RNA, Viral</term>
<term>Ribavirin</term>
<term>Viral Nonstructural Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term>
<term>Ribavirin</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term>
<term>Coronavirus</term>
<term>Exoribonucleases</term>
<term>Methyltransferases</term>
<term>Protéines virales non structurales</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term>
<term>Antiviraux</term>
<term>Coronavirus</term>
<term>Exoribonucleases</term>
<term>Methyltransferases</term>
<term>Protéines virales non structurales</term>
<term>Ribavirine</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term>
<term>Ribavirine</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Crystallography, X-Ray</term>
<term>Humans</term>
<term>Models, Molecular</term>
<term>Protein Binding</term>
<term>Protein Domains</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Coronavirus</term>
<term>Cristallographie aux rayons X</term>
<term>Domaines protéiques</term>
<term>Exoribonucleases</term>
<term>Humains</term>
<term>Liaison aux protéines</term>
<term>Methyltransferases</term>
<term>Modèles moléculaires</term>
<term>Protéines virales non structurales</term>
<term>Réplication virale</term>
</keywords>
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<front><div type="abstract" xml:lang="en"><title>Significance</title>
<p>Emerging coronaviruses (CoVs; severe acute respiratory syndrome-CoV and Middle East respiratory syndrome-CoV) pose serious health threats globally, with no specific antiviral treatments available. These viruses are able to faithfully synthesize their large genomic RNA. We report, however, that their main RNA polymerase, nsp12, is not accurate. To achieve accuracy, CoVs have acquired nsp14, a bifunctional enzyme able to methylate the viral RNA cap [methyltransferase (MTase)] and excise erroneous mutagenic nucleotides inserted by nsp12. Strikingly, ribavirin can be excised from the viral genome, thus showing no antiviral activity. The crystal structure of nsp14 shows that it is unique, having been replaced by other MTase types during evolution. This unprecedented RNA correction machinery has allowed RNA genome size expansion, but also provided potential nucleoside drug resistance to these deadly pathogens.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Drake, Jw" uniqKey="Drake J">JW Drake</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Belshaw, R" uniqKey="Belshaw R">R Belshaw</name>
</author>
<author><name sortKey="Gardner, A" uniqKey="Gardner A">A Gardner</name>
</author>
<author><name sortKey="Rambaut, A" uniqKey="Rambaut A">A Rambaut</name>
</author>
<author><name sortKey="Pybus, Og" uniqKey="Pybus O">OG Pybus</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sanjuan, R" uniqKey="Sanjuan R">R Sanjuán</name>
</author>
<author><name sortKey="Nebot, Mr" uniqKey="Nebot M">MR Nebot</name>
</author>
<author><name sortKey="Chirico, N" uniqKey="Chirico N">N Chirico</name>
</author>
<author><name sortKey="Mansky, Lm" uniqKey="Mansky L">LM Mansky</name>
</author>
<author><name sortKey="Belshaw, R" uniqKey="Belshaw R">R Belshaw</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sidwell, Rw" uniqKey="Sidwell R">RW Sidwell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Parker, Wb" uniqKey="Parker W">WB Parker</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crotty, S" uniqKey="Crotty S">S Crotty</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mchutchison, Jg" uniqKey="Mchutchison J">JG McHutchison</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Hall, Cb" uniqKey="Hall C">CB Hall</name>
</author>
<author><name sortKey="Walsh, Ee" uniqKey="Walsh E">EE Walsh</name>
</author>
<author><name sortKey="Hruska, Jf" uniqKey="Hruska J">JF Hruska</name>
</author>
<author><name sortKey="Betts, Rf" uniqKey="Betts R">RF Betts</name>
</author>
<author><name sortKey="Hall, Wj" uniqKey="Hall W">WJ Hall</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mccormick, Jb" uniqKey="Mccormick J">JB McCormick</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stockman, Lj" uniqKey="Stockman L">LJ Stockman</name>
</author>
<author><name sortKey="Bellamy, R" uniqKey="Bellamy R">R Bellamy</name>
</author>
<author><name sortKey="Garner, P" uniqKey="Garner P">P Garner</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Al Tawfiq, Ja" uniqKey="Al Tawfiq J">JA Al-Tawfiq</name>
</author>
<author><name sortKey="Memish, Za" uniqKey="Memish Z">ZA Memish</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Stadler, K" uniqKey="Stadler K">K Stadler</name>
</author>
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<author><name sortKey="Rosario, D" uniqKey="Rosario D">D Rosario</name>
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</author>
<author><name sortKey="Snijder, Ej" uniqKey="Snijder E">EJ Snijder</name>
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</author>
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</author>
<author><name sortKey="Deval, J" uniqKey="Deval J">J Deval</name>
</author>
<author><name sortKey="Johnson, Ka" uniqKey="Johnson K">KA Johnson</name>
</author>
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<author><name sortKey="Charlesworth, D" uniqKey="Charlesworth D">D Charlesworth</name>
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<author><name sortKey="Crow, Jf" uniqKey="Crow J">JF Crow</name>
</author>
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</author>
<author><name sortKey="Decroly, E" uniqKey="Decroly E">E Decroly</name>
</author>
<author><name sortKey="Ziebuhr, J" uniqKey="Ziebuhr J">J Ziebuhr</name>
</author>
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</author>
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</author>
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</author>
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<biblStruct><analytic><author><name sortKey="Vonrhein, C" uniqKey="Vonrhein C">C Vonrhein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vonrhein, C" uniqKey="Vonrhein C">C Vonrhein</name>
</author>
<author><name sortKey="Blanc, E" uniqKey="Blanc E">E Blanc</name>
</author>
<author><name sortKey="Roversi, P" uniqKey="Roversi P">P Roversi</name>
</author>
<author><name sortKey="Bricogne, G" uniqKey="Bricogne G">G Bricogne</name>
</author>
</analytic>
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</author>
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</author>
<author><name sortKey="Cowtan, K" uniqKey="Cowtan K">K Cowtan</name>
</author>
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</author>
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</author>
</analytic>
</biblStruct>
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</author>
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</author>
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</biblStruct>
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</div1>
</back>
</TEI>
</PMC>
</double>
</record>

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Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA

Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA

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