Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA.
Identifieur interne : 000A39 ( PubMed/Corpus ); précédent : 000A38; suivant : 000A40Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA.
Auteurs : François Ferron ; Lorenzo Subissi ; Ana Theresa Silveira De Morais ; Nhung Thi Tuyet Le ; Marion Sevajol ; Laure Gluais ; Etienne Decroly ; Clemens Vonrhein ; Gérard Bricogne ; Bruno Canard ; Isabelle ImbertSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 1091-6490 ] ; 2018.
English descriptors
- KwdEn :
- Antiviral Agents (metabolism), Antiviral Agents (pharmacology), Coronavirus (drug effects), Coronavirus (genetics), Coronavirus (metabolism), Crystallography, X-Ray, Exoribonucleases (chemistry), Exoribonucleases (genetics), Exoribonucleases (metabolism), Humans, Methyltransferases (chemistry), Methyltransferases (genetics), Methyltransferases (metabolism), Models, Molecular, Protein Binding, Protein Domains, RNA, Viral (genetics), RNA, Viral (metabolism), Ribavirin (metabolism), Ribavirin (pharmacology), Severe Acute Respiratory Syndrome (virology), Viral Nonstructural Proteins (chemistry), Viral Nonstructural Proteins (genetics), Viral Nonstructural Proteins (metabolism), Virus Replication.
- MESH :
- chemical , chemistry : Exoribonucleases, Methyltransferases, Viral Nonstructural Proteins.
- chemical , genetics : Exoribonucleases, Methyltransferases, RNA, Viral, Viral Nonstructural Proteins.
- chemical , metabolism : Antiviral Agents, Exoribonucleases, Methyltransferases, RNA, Viral, Ribavirin, Viral Nonstructural Proteins.
- chemical , pharmacology : Antiviral Agents, Ribavirin.
- drug effects : Coronavirus.
- genetics : Coronavirus.
- metabolism : Coronavirus.
- virology : Severe Acute Respiratory Syndrome.
- Crystallography, X-Ray, Humans, Models, Molecular, Protein Binding, Protein Domains, Virus Replication.
Abstract
Coronaviruses (CoVs) stand out among RNA viruses because of their unusually large genomes (∼30 kb) associated with low mutation rates. CoVs code for nsp14, a bifunctional enzyme carrying RNA cap guanine N7-methyltransferase (MTase) and 3'-5' exoribonuclease (ExoN) activities. ExoN excises nucleotide mismatches at the RNA 3'-end in vitro, and its inactivation in vivo jeopardizes viral genetic stability. Here, we demonstrate for severe acute respiratory syndrome (SARS)-CoV an RNA synthesis and proofreading pathway through association of nsp14 with the low-fidelity nsp12 viral RNA polymerase. Through this pathway, the antiviral compound ribavirin 5'-monophosphate is significantly incorporated but also readily excised from RNA, which may explain its limited efficacy in vivo. The crystal structure at 3.38 Å resolution of SARS-CoV nsp14 in complex with its cofactor nsp10 adds to the uniqueness of CoVs among RNA viruses: The MTase domain presents a new fold that differs sharply from the canonical Rossmann fold.
DOI: 10.1073/pnas.1718806115
PubMed: 29279395
Links to Exploration step
pubmed:29279395Le document en format XML
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isabelle.imbert@univ-amu.fr.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29279395</idno><idno type="pmid">29279395</idno><idno type="doi">10.1073/pnas.1718806115</idno><idno type="wicri:Area/PubMed/Corpus">000A39</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000A39</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA.</title><author><name sortKey="Ferron, Francois" sort="Ferron, Francois" uniqKey="Ferron F" first="François" last="Ferron">François Ferron</name><affiliation><nlm:affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Subissi, Lorenzo" sort="Subissi, Lorenzo" uniqKey="Subissi L" first="Lorenzo" last="Subissi">Lorenzo Subissi</name><affiliation><nlm:affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Silveira De Morais, Ana Theresa" sort="Silveira De Morais, Ana Theresa" uniqKey="Silveira De Morais A" first="Ana Theresa" last="Silveira De Morais">Ana Theresa Silveira De Morais</name><affiliation><nlm:affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Le, Nhung Thi Tuyet" sort="Le, Nhung Thi Tuyet" uniqKey="Le N" first="Nhung Thi Tuyet" last="Le">Nhung Thi Tuyet Le</name><affiliation><nlm:affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Sevajol, Marion" sort="Sevajol, Marion" uniqKey="Sevajol M" first="Marion" last="Sevajol">Marion Sevajol</name><affiliation><nlm:affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Gluais, Laure" sort="Gluais, Laure" uniqKey="Gluais L" first="Laure" last="Gluais">Laure Gluais</name><affiliation><nlm:affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Decroly, Etienne" sort="Decroly, Etienne" uniqKey="Decroly E" first="Etienne" last="Decroly">Etienne Decroly</name><affiliation><nlm:affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Vonrhein, Clemens" sort="Vonrhein, Clemens" uniqKey="Vonrhein C" first="Clemens" last="Vonrhein">Clemens Vonrhein</name><affiliation><nlm:affiliation>Global Phasing Ltd., Cambridge CB3 0AX, England.</nlm:affiliation></affiliation></author><author><name sortKey="Bricogne, Gerard" sort="Bricogne, Gerard" uniqKey="Bricogne G" first="Gérard" last="Bricogne">Gérard Bricogne</name><affiliation><nlm:affiliation>Global Phasing Ltd., Cambridge CB3 0AX, England.</nlm:affiliation></affiliation></author><author><name sortKey="Canard, Bruno" sort="Canard, Bruno" uniqKey="Canard B" first="Bruno" last="Canard">Bruno Canard</name><affiliation><nlm:affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</nlm:affiliation></affiliation></author><author><name sortKey="Imbert, Isabelle" sort="Imbert, Isabelle" uniqKey="Imbert I" first="Isabelle" last="Imbert">Isabelle Imbert</name><affiliation><nlm:affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France; isabelle.imbert@univ-amu.fr.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="eISSN">1091-6490</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antiviral Agents (metabolism)</term><term>Antiviral Agents (pharmacology)</term><term>Coronavirus (drug effects)</term><term>Coronavirus (genetics)</term><term>Coronavirus (metabolism)</term><term>Crystallography, X-Ray</term><term>Exoribonucleases (chemistry)</term><term>Exoribonucleases (genetics)</term><term>Exoribonucleases (metabolism)</term><term>Humans</term><term>Methyltransferases (chemistry)</term><term>Methyltransferases (genetics)</term><term>Methyltransferases (metabolism)</term><term>Models, Molecular</term><term>Protein Binding</term><term>Protein Domains</term><term>RNA, Viral (genetics)</term><term>RNA, Viral (metabolism)</term><term>Ribavirin (metabolism)</term><term>Ribavirin (pharmacology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Viral Nonstructural Proteins (chemistry)</term><term>Viral Nonstructural Proteins (genetics)</term><term>Viral Nonstructural Proteins (metabolism)</term><term>Virus Replication</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Exoribonucleases</term><term>Methyltransferases</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Exoribonucleases</term><term>Methyltransferases</term><term>RNA, Viral</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antiviral Agents</term><term>Exoribonucleases</term><term>Methyltransferases</term><term>RNA, Viral</term><term>Ribavirin</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Ribavirin</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Crystallography, X-Ray</term><term>Humans</term><term>Models, Molecular</term><term>Protein Binding</term><term>Protein Domains</term><term>Virus Replication</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Coronaviruses (CoVs) stand out among RNA viruses because of their unusually large genomes (∼30 kb) associated with low mutation rates. CoVs code for nsp14, a bifunctional enzyme carrying RNA cap guanine N7-methyltransferase (MTase) and 3'-5' exoribonuclease (ExoN) activities. ExoN excises nucleotide mismatches at the RNA 3'-end in vitro, and its inactivation in vivo jeopardizes viral genetic stability. Here, we demonstrate for severe acute respiratory syndrome (SARS)-CoV an RNA synthesis and proofreading pathway through association of nsp14 with the low-fidelity nsp12 viral RNA polymerase. Through this pathway, the antiviral compound ribavirin 5'-monophosphate is significantly incorporated but also readily excised from RNA, which may explain its limited efficacy in vivo. The crystal structure at 3.38 Å resolution of SARS-CoV nsp14 in complex with its cofactor nsp10 adds to the uniqueness of CoVs among RNA viruses: The MTase domain presents a new fold that differs sharply from the canonical Rossmann fold.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29279395</PMID><DateCompleted><Year>2018</Year><Month>07</Month><Day>17</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1091-6490</ISSN><JournalIssue CitedMedium="Internet"><Volume>115</Volume><Issue>2</Issue><PubDate><Year>2018</Year><Month>01</Month><Day>09</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc. Natl. Acad. Sci. U.S.A.</ISOAbbreviation></Journal><ArticleTitle>Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA.</ArticleTitle><Pagination><MedlinePgn>E162-E171</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1073/pnas.1718806115</ELocationID><Abstract><AbstractText>Coronaviruses (CoVs) stand out among RNA viruses because of their unusually large genomes (∼30 kb) associated with low mutation rates. CoVs code for nsp14, a bifunctional enzyme carrying RNA cap guanine N7-methyltransferase (MTase) and 3'-5' exoribonuclease (ExoN) activities. ExoN excises nucleotide mismatches at the RNA 3'-end in vitro, and its inactivation in vivo jeopardizes viral genetic stability. Here, we demonstrate for severe acute respiratory syndrome (SARS)-CoV an RNA synthesis and proofreading pathway through association of nsp14 with the low-fidelity nsp12 viral RNA polymerase. Through this pathway, the antiviral compound ribavirin 5'-monophosphate is significantly incorporated but also readily excised from RNA, which may explain its limited efficacy in vivo. The crystal structure at 3.38 Å resolution of SARS-CoV nsp14 in complex with its cofactor nsp10 adds to the uniqueness of CoVs among RNA viruses: The MTase domain presents a new fold that differs sharply from the canonical Rossmann fold.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ferron</LastName><ForeName>François</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Subissi</LastName><ForeName>Lorenzo</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Silveira De Morais</LastName><ForeName>Ana Theresa</ForeName><Initials>AT</Initials><AffiliationInfo><Affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Le</LastName><ForeName>Nhung Thi Tuyet</ForeName><Initials>NTT</Initials><AffiliationInfo><Affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sevajol</LastName><ForeName>Marion</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gluais</LastName><ForeName>Laure</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Decroly</LastName><ForeName>Etienne</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Vonrhein</LastName><ForeName>Clemens</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Global Phasing Ltd., Cambridge CB3 0AX, England.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bricogne</LastName><ForeName>Gérard</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Global Phasing Ltd., Cambridge CB3 0AX, England.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Canard</LastName><ForeName>Bruno</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Imbert</LastName><ForeName>Isabelle</ForeName><Initials>I</Initials><Identifier Source="ORCID">0000-0001-5630-9945</Identifier><AffiliationInfo><Affiliation>Centre National de la Recherche Scientifique, Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 13009 Marseille, France; isabelle.imbert@univ-amu.fr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>PDB</DataBankName><AccessionNumberList><AccessionNumber>5NFY</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>12</Month><Day>26</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000998">Antiviral Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C558878">Nsp10 protein, SARS virus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017361">Viral Nonstructural Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>49717AWG6K</RegistryNumber><NameOfSubstance UI="D012254">Ribavirin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.-</RegistryNumber><NameOfSubstance UI="D008780">Methyltransferases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.1.1.56</RegistryNumber><NameOfSubstance UI="C525596">nsp14 protein, SARS coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.-</RegistryNumber><NameOfSubstance UI="D005095">Exoribonucleases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000998" MajorTopicYN="N">Antiviral Agents</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017934" MajorTopicYN="N">Coronavirus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018360" MajorTopicYN="N">Crystallography, X-Ray</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005095" MajorTopicYN="N">Exoribonucleases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008780" MajorTopicYN="N">Methyltransferases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008958" MajorTopicYN="N">Models, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000072417" MajorTopicYN="N">Protein Domains</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012254" MajorTopicYN="N">Ribavirin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017361" MajorTopicYN="N">Viral Nonstructural Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="Y">Virus Replication</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">RNA virus</Keyword><Keyword MajorTopicYN="Y">fold evolution</Keyword><Keyword MajorTopicYN="Y">proofreading</Keyword><Keyword MajorTopicYN="Y">ribavirin</Keyword><Keyword MajorTopicYN="Y">virus replication</Keyword></KeywordList><CoiStatement>The authors declare no conflict of interest.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>12</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>7</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>12</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29279395</ArticleId><ArticleId IdType="pii">1718806115</ArticleId><ArticleId IdType="doi">10.1073/pnas.1718806115</ArticleId><ArticleId IdType="pmc">PMC5777078</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nat Rev Microbiol. 2003 Dec;1(3):209-18</Citation><ArticleIdList><ArticleId IdType="pubmed">15035025</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2006 Apr;117(1):17-37</Citation><ArticleIdList><ArticleId IdType="pubmed">16503362</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2010 May 06;6(5):e1000896</Citation><ArticleIdList><ArticleId IdType="pubmed">20463816</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1993 May 1;90(9):4171-5</Citation><ArticleIdList><ArticleId IdType="pubmed">8387212</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2006 Sep;80(18):9192-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16940530</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2003 Aug 29;331(5):991-1004</Citation><ArticleIdList><ArticleId IdType="pubmed">12927536</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 1998 Nov 19;339(21):1485-92</Citation><ArticleIdList><ArticleId IdType="pubmed">9819446</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2006 Aug;80(16):7894-901</Citation><ArticleIdList><ArticleId IdType="pubmed">16873246</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2012 Nov 8;367 (19):1814-20</Citation><ArticleIdList><ArticleId IdType="pubmed">23075143</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Med. 2000 Dec;6(12):1375-9</Citation><ArticleIdList><ArticleId IdType="pubmed">11100123</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Exp Med Biol. 2012;725:159-83</Citation><ArticleIdList><ArticleId IdType="pubmed">22399324</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):12-21</Citation><ArticleIdList><ArticleId IdType="pubmed">20057044</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2015 Jul 28;112(30):9436-41</Citation><ArticleIdList><ArticleId IdType="pubmed">26159422</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Transl Med. 2017 Jun 28;9(396):null</Citation><ArticleIdList><ArticleId IdType="pubmed">28659436</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2014 Sep 16;111(37):E3900-9</Citation><ArticleIdList><ArticleId IdType="pubmed">25197083</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Rev Microbiol. 2009 Jun;7(6):439-50</Citation><ArticleIdList><ArticleId IdType="pubmed">19430490</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1988 Apr 8;240(4849):199-201</Citation><ArticleIdList><ArticleId IdType="pubmed">2832946</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Nov;81(22):12135-44</Citation><ArticleIdList><ArticleId IdType="pubmed">17804504</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 2000 Aug;6(2):349-60</Citation><ArticleIdList><ArticleId IdType="pubmed">10983982</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 1973 May 15;76(2):241-56</Citation><ArticleIdList><ArticleId IdType="pubmed">4737475</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2010 Oct;84(19):9733-48</Citation><ArticleIdList><ArticleId IdType="pubmed">20660197</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1002-11</Citation><ArticleIdList><ArticleId IdType="pubmed">16929101</ArticleId></ArticleIdList></Reference><Reference><Citation>Expert Rev Anti Infect Ther. 2017 Mar;15(3):269-275</Citation><ArticleIdList><ArticleId IdType="pubmed">27937060</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5108-13</Citation><ArticleIdList><ArticleId IdType="pubmed">16549795</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2011 May;7(5):e1002059</Citation><ArticleIdList><ArticleId IdType="pubmed">21637813</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2006 Aug 8;103(32):11892-7</Citation><ArticleIdList><ArticleId IdType="pubmed">16882730</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2013 Aug;9(8):e1003565</Citation><ArticleIdList><ArticleId IdType="pubmed">23966862</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2005 Feb;107(2):165-71</Citation><ArticleIdList><ArticleId IdType="pubmed">15649562</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2010 Apr 22;6(4):e1000863</Citation><ArticleIdList><ArticleId IdType="pubmed">20421945</ArticleId></ArticleIdList></Reference><Reference><Citation>J Comput Chem. 2004 Oct;25(13):1605-12</Citation><ArticleIdList><ArticleId IdType="pubmed">15264254</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2012 Jun 12;109(24):9372-7</Citation><ArticleIdList><ArticleId IdType="pubmed">22635272</ArticleId></ArticleIdList></Reference><Reference><Citation>Nucleic Acids Res. 2014 Oct;42(18):11642-56</Citation><ArticleIdList><ArticleId IdType="pubmed">25209234</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2006 Aug;80(16):7902-8</Citation><ArticleIdList><ArticleId IdType="pubmed">16873247</ArticleId></ArticleIdList></Reference><Reference><Citation>Genetics. 1998 Apr;148(4):1667-86</Citation><ArticleIdList><ArticleId IdType="pubmed">9560386</ArticleId></ArticleIdList></Reference><Reference><Citation>Virus Res. 2008 May;133(2):136-48</Citation><ArticleIdList><ArticleId IdType="pubmed">18255185</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2017 Feb 3;13(2):e1006195</Citation><ArticleIdList><ArticleId IdType="pubmed">28158275</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Med. 2006 Sep;3(9):e343</Citation><ArticleIdList><ArticleId IdType="pubmed">16968120</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 1972 Aug 25;177(4050):705-6</Citation><ArticleIdList><ArticleId IdType="pubmed">4340949</ArticleId></ArticleIdList></Reference><Reference><Citation>Adv Virus Res. 2016;96:59-126</Citation><ArticleIdList><ArticleId IdType="pubmed">27712628</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Ecol Evol. 2008 Apr;23(4):188-93</Citation><ArticleIdList><ArticleId IdType="pubmed">18295930</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2011 Jan 21;286(3):2067-77</Citation><ArticleIdList><ArticleId IdType="pubmed">21078673</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Mol Biol Educ. 2015 May-Jun;43(3):206-9</Citation><ArticleIdList><ArticleId IdType="pubmed">25704928</ArticleId></ArticleIdList></Reference><Reference><Citation>Structure. 2007 Apr;15(4):417-28</Citation><ArticleIdList><ArticleId IdType="pubmed">17437714</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 1986 Jan 2;314(1):20-6</Citation><ArticleIdList><ArticleId IdType="pubmed">3940312</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2014 Sep 12;289(37):25783-96</Citation><ArticleIdList><ArticleId IdType="pubmed">25074927</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2013;9(7):e1003500</Citation><ArticleIdList><ArticleId IdType="pubmed">23874204</ArticleId></ArticleIdList></Reference><Reference><Citation>J Mol Biol. 2004 Oct 15;343(2):305-12</Citation><ArticleIdList><ArticleId IdType="pubmed">15451662</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2210-21</Citation><ArticleIdList><ArticleId IdType="pubmed">15572774</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):293-302</Citation><ArticleIdList><ArticleId IdType="pubmed">21460447</ArticleId></ArticleIdList></Reference><Reference><Citation>JAMA. 1983 May 20;249(19):2666-70</Citation><ArticleIdList><ArticleId IdType="pubmed">6341640</ArticleId></ArticleIdList></Reference><Reference><Citation>Nat Methods. 2013 Nov;10(11):1102-4</Citation><ArticleIdList><ArticleId IdType="pubmed">24076763</ArticleId></ArticleIdList></Reference><Reference><Citation>Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32</Citation><ArticleIdList><ArticleId IdType="pubmed">15572765</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2010 Dec 9;468(7325):779-83</Citation><ArticleIdList><ArticleId IdType="pubmed">21085117</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2013 Jun;87(11):6296-305</Citation><ArticleIdList><ArticleId IdType="pubmed">23536667</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2009 Mar 3;106(9):3484-9</Citation><ArticleIdList><ArticleId IdType="pubmed">19208801</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2007 Dec;81(24):13587-97</Citation><ArticleIdList><ArticleId IdType="pubmed">17898055</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2016 Mar 17;531(7594):381-5</Citation><ArticleIdList><ArticleId IdType="pubmed">26934220</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2011 Oct;7(10):e1002294</Citation><ArticleIdList><ArticleId IdType="pubmed">22022266</ArticleId></ArticleIdList></Reference><Reference><Citation>J Virol. 2016 May 12;90(11):5399-414</Citation><ArticleIdList><ArticleId IdType="pubmed">27009949</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2007;364:215-30</Citation><ArticleIdList><ArticleId IdType="pubmed">17172768</ArticleId></ArticleIdList></Reference><Reference><Citation>RNA Biol. 2014;11(12):1597-607</Citation><ArticleIdList><ArticleId 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