Detecting respiratory viral RNA using expanded genetic alphabets and self-avoiding DNA.
Identifieur interne : 001334 ( Main/Exploration ); précédent : 001333; suivant : 001335Detecting respiratory viral RNA using expanded genetic alphabets and self-avoiding DNA.
Auteurs : Lyudmyla G. Glushakova [États-Unis] ; Nidhi Sharma [États-Unis] ; Shuichi Hoshika [États-Unis] ; Andrea C. Bradley [États-Unis] ; Kevin M. Bradley [États-Unis] ; Zunyi Yang [États-Unis] ; Steven A. Benner [États-Unis]Source :
- Analytical biochemistry [ 1096-0309 ] ; 2015.
Descripteurs français
- KwdFr :
- ADN (métabolisme), ADN simple brin (métabolisme), ARN viral (isolement et purification), ARN viral (métabolisme), Acides nucléiques immobilisés (métabolisme), Biologie synthétique (), Colorants fluorescents (), Coronaviridae (), Coronaviridae (isolement et purification), Coronaviridae (métabolisme), DNA-directed RNA polymerases (métabolisme), Désoxyribonucléosides (métabolisme), Hybridation d'acides nucléiques (), Hétéroduplexes d'acides nucléiques, Infections de l'appareil respiratoire (diagnostic), Infections de l'appareil respiratoire (virologie), Liaison hydrogène, Limite de détection, Microsphères, Orthomyxoviridae (), Orthomyxoviridae (isolement et purification), Orthomyxoviridae (métabolisme), Phycoérythrine (), Protéines virales (métabolisme), Pyridones (métabolisme), RT-PCR, Réaction de polymérisation en chaine multiplex (), Triazines (métabolisme), Typage moléculaire (), Virus respiratoires syncytiaux (), Virus respiratoires syncytiaux (isolement et purification), Virus respiratoires syncytiaux (métabolisme).
- MESH :
- diagnostic : Infections de l'appareil respiratoire.
- isolement et purification : ARN viral, Coronaviridae, Orthomyxoviridae, Virus respiratoires syncytiaux.
- métabolisme : ADN, ADN simple brin, ARN viral, Acides nucléiques immobilisés, Coronaviridae, DNA-directed RNA polymerases, Désoxyribonucléosides, Orthomyxoviridae, Protéines virales, Pyridones, Triazines, Virus respiratoires syncytiaux.
- virologie : Infections de l'appareil respiratoire.
- Biologie synthétique, Colorants fluorescents, Coronaviridae, Hybridation d'acides nucléiques, Hétéroduplexes d'acides nucléiques, Liaison hydrogène, Limite de détection, Microsphères, Orthomyxoviridae, Phycoérythrine, RT-PCR, Réaction de polymérisation en chaine multiplex, Typage moléculaire, Virus respiratoires syncytiaux.
English descriptors
- KwdEn :
- Coronaviridae (classification), Coronaviridae (isolation & purification), Coronaviridae (metabolism), DNA (metabolism), DNA, Single-Stranded (metabolism), DNA-Directed RNA Polymerases (metabolism), Deoxyribonucleosides (metabolism), Fluorescent Dyes (chemistry), Hydrogen Bonding, Immobilized Nucleic Acids (metabolism), Limit of Detection, Microspheres, Molecular Typing (methods), Multiplex Polymerase Chain Reaction (methods), Nucleic Acid Heteroduplexes, Nucleic Acid Hybridization (methods), Orthomyxoviridae (classification), Orthomyxoviridae (isolation & purification), Orthomyxoviridae (metabolism), Phycoerythrin (chemistry), Pyridones (metabolism), RNA, Viral (isolation & purification), RNA, Viral (metabolism), Respiratory Syncytial Viruses (classification), Respiratory Syncytial Viruses (isolation & purification), Respiratory Syncytial Viruses (metabolism), Respiratory Tract Infections (diagnosis), Respiratory Tract Infections (virology), Reverse Transcriptase Polymerase Chain Reaction, Synthetic Biology (methods), Triazines (metabolism), Viral Proteins (metabolism).
- MESH :
- chemical , chemistry : Fluorescent Dyes, Phycoerythrin.
- chemical , isolation & purification : RNA, Viral.
- chemical , metabolism : DNA, DNA, Single-Stranded, DNA-Directed RNA Polymerases, Deoxyribonucleosides, Immobilized Nucleic Acids, Pyridones, RNA, Viral, Triazines, Viral Proteins.
- classification : Coronaviridae, Orthomyxoviridae, Respiratory Syncytial Viruses.
- diagnosis : Respiratory Tract Infections.
- isolation & purification : Coronaviridae, Orthomyxoviridae, Respiratory Syncytial Viruses.
- metabolism : Coronaviridae, Orthomyxoviridae, Respiratory Syncytial Viruses.
- methods : Molecular Typing, Multiplex Polymerase Chain Reaction, Nucleic Acid Hybridization, Synthetic Biology.
- virology : Respiratory Tract Infections.
- Hydrogen Bonding, Limit of Detection, Microspheres, Nucleic Acid Heteroduplexes, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Nucleic acid (NA)-targeted tests detect and quantify viral DNA and RNA (collectively xNA) to support epidemiological surveillance and, in individual patients, to guide therapy. They commonly use polymerase chain reaction (PCR) and reverse transcription PCR. Although these all have rapid turnaround, they are expensive to run. Multiplexing would allow their cost to be spread over multiple targets, but often only with lower sensitivity and accuracy, noise, false positives, and false negatives; these arise by interactions between the multiple nucleic acid primers and probes in a multiplexed kit. Here we offer a multiplexed assay for a panel of respiratory viruses that mitigates these problems by combining several nucleic acid analogs from the emerging field of synthetic biology: (i) self-avoiding molecular recognition systems (SAMRSs), which facilitate multiplexing, and (ii) artificially expanded genetic information systems (AEGISs), which enable low-noise PCR. These are supplemented by "transliteration" technology, which converts standard nucleotides in a target to AEGIS nucleotides in a product, improving hybridization. The combination supports a multiplexed Luminex-based respiratory panel that potentially differentiates influenza viruses A and B, respiratory syncytial virus, severe acute respiratory syndrome coronavirus (SARS), and Middle East respiratory syndrome (MERS) coronavirus, detecting as few as 10 MERS virions in a 20-μl sample.
DOI: 10.1016/j.ab.2015.08.015
PubMed: 26299645
Affiliations:
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Le document en format XML
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<term>Coronaviridae (metabolism)</term>
<term>DNA (metabolism)</term>
<term>DNA, Single-Stranded (metabolism)</term>
<term>DNA-Directed RNA Polymerases (metabolism)</term>
<term>Deoxyribonucleosides (metabolism)</term>
<term>Fluorescent Dyes (chemistry)</term>
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<term>Multiplex Polymerase Chain Reaction (methods)</term>
<term>Nucleic Acid Heteroduplexes</term>
<term>Nucleic Acid Hybridization (methods)</term>
<term>Orthomyxoviridae (classification)</term>
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<term>Orthomyxoviridae (metabolism)</term>
<term>Phycoerythrin (chemistry)</term>
<term>Pyridones (metabolism)</term>
<term>RNA, Viral (isolation & purification)</term>
<term>RNA, Viral (metabolism)</term>
<term>Respiratory Syncytial Viruses (classification)</term>
<term>Respiratory Syncytial Viruses (isolation & purification)</term>
<term>Respiratory Syncytial Viruses (metabolism)</term>
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<term>Respiratory Tract Infections (virology)</term>
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<term>Infections de l'appareil respiratoire (virologie)</term>
<term>Liaison hydrogène</term>
<term>Limite de détection</term>
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<front><div type="abstract" xml:lang="en">Nucleic acid (NA)-targeted tests detect and quantify viral DNA and RNA (collectively xNA) to support epidemiological surveillance and, in individual patients, to guide therapy. They commonly use polymerase chain reaction (PCR) and reverse transcription PCR. Although these all have rapid turnaround, they are expensive to run. Multiplexing would allow their cost to be spread over multiple targets, but often only with lower sensitivity and accuracy, noise, false positives, and false negatives; these arise by interactions between the multiple nucleic acid primers and probes in a multiplexed kit. Here we offer a multiplexed assay for a panel of respiratory viruses that mitigates these problems by combining several nucleic acid analogs from the emerging field of synthetic biology: (i) self-avoiding molecular recognition systems (SAMRSs), which facilitate multiplexing, and (ii) artificially expanded genetic information systems (AEGISs), which enable low-noise PCR. These are supplemented by "transliteration" technology, which converts standard nucleotides in a target to AEGIS nucleotides in a product, improving hybridization. The combination supports a multiplexed Luminex-based respiratory panel that potentially differentiates influenza viruses A and B, respiratory syncytial virus, severe acute respiratory syndrome coronavirus (SARS), and Middle East respiratory syndrome (MERS) coronavirus, detecting as few as 10 MERS virions in a 20-μl sample. </div>
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<name sortKey="Sharma, Nidhi" sort="Sharma, Nidhi" uniqKey="Sharma N" first="Nidhi" last="Sharma">Nidhi Sharma</name>
<name sortKey="Yang, Zunyi" sort="Yang, Zunyi" uniqKey="Yang Z" first="Zunyi" last="Yang">Zunyi Yang</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
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HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001334 | SxmlIndent | more
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Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:26299645 |texte= Detecting respiratory viral RNA using expanded genetic alphabets and self-avoiding DNA. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26299645" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
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