Validation of assays to monitor immune responses in the Syrian golden hamster (Mesocricetus auratus).
Identifieur interne : 002013 ( Main/Exploration ); précédent : 002012; suivant : 002014Validation of assays to monitor immune responses in the Syrian golden hamster (Mesocricetus auratus).
Auteurs : Marko Zivcec [États-Unis] ; David Safronetz ; Elaine Haddock ; Heinz Feldmann ; Hideki EbiharaSource :
- Journal of immunological methods [ 1872-7905 ] ; 2011.
Descripteurs français
- KwdFr :
- ARN (), ARN (génétique), Animaux, Cricetinae, Immunité innée (génétique), Immunité innée (immunologie), Lipopolysaccharides (pharmacologie), Mesocricetus (génétique), Mesocricetus (immunologie), RT-PCR (), RT-PCR (médecine vétérinaire), RT-PCR (normes), Virus de la stomatite vésiculeuse de type Indiana (immunologie).
- MESH :
- génétique : ARN, Immunité innée, Mesocricetus.
- immunologie : Immunité innée, Mesocricetus, Virus de la stomatite vésiculeuse de type Indiana.
- médecine vétérinaire : RT-PCR.
- normes : RT-PCR.
- pharmacologie : Lipopolysaccharides.
- ARN, Animaux, Cricetinae, RT-PCR.
English descriptors
- KwdEn :
- Animals, Cricetinae, Immunity, Innate (genetics), Immunity, Innate (immunology), Lipopolysaccharides (pharmacology), Mesocricetus (genetics), Mesocricetus (immunology), RNA (chemistry), RNA (genetics), Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (standards), Reverse Transcriptase Polymerase Chain Reaction (veterinary), Vesicular stomatitis Indiana virus (immunology).
- MESH :
- chemical , chemistry : RNA.
- chemical , genetics : RNA.
- chemical , pharmacology : Lipopolysaccharides.
- genetics : Immunity, Innate, Mesocricetus.
- immunology : Immunity, Innate, Mesocricetus, Vesicular stomatitis Indiana virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- standards : Reverse Transcriptase Polymerase Chain Reaction.
- veterinary : Reverse Transcriptase Polymerase Chain Reaction.
- Animals, Cricetinae.
Abstract
The Syrian golden hamster (Mesocricetus auratus) is a valuable but under-utilized animal model for studies of human viral pathogens such as bunyaviruses, arenaviruses, flaviviruses, henipaviruses, and SARS-coronavirus. A lack of suitable reagents and specific assays for monitoring host responses has limited the use of this animal model to clinical observations, pathology and humoral immune responses. The objective of this study was to establish and validate assays to monitor host immune responses in the hamster including important pro-inflammatory, anti-inflammatory and innate immune responses, as well as markers of apoptosis, cell proliferation, cell junction integrity and coagulation. Commercially available mouse and rat ELISA and luminex panels were screened for potential cross-reactivity, but were found to be of limited value for studying host responses in hamsters. Subsequently, quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays for the detection of 51 immune-related and four internal reference genes were developed. To validate the immune-related assays, hamsters were infected with vesicular stomatitis virus (VSV), Indiana species, or treated with lipopolysaccharide (LPS) and host immune responses were monitored in selected organs. Ribosomal protein L18 was identified as the most stable internal reference gene. In conclusion, these new assays will greatly improve the use of the hamster as an important small animal model in infectious disease research.
DOI: 10.1016/j.jim.2011.02.004
PubMed: 21334343
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001546
- to stream PubMed, to step Curation: 001546
- to stream PubMed, to step Checkpoint: 001405
- to stream Ncbi, to step Merge: 002297
- to stream Ncbi, to step Curation: 002297
- to stream Ncbi, to step Checkpoint: 002297
- to stream Main, to step Merge: 002037
- to stream Main, to step Curation: 002013
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Validation of assays to monitor immune responses in the Syrian golden hamster (Mesocricetus auratus).</title><author><name sortKey="Zivcec, Marko" sort="Zivcec, Marko" uniqKey="Zivcec M" first="Marko" last="Zivcec">Marko Zivcec</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Safronetz, David" sort="Safronetz, David" uniqKey="Safronetz D" first="David" last="Safronetz">David Safronetz</name></author><author><name sortKey="Haddock, Elaine" sort="Haddock, Elaine" uniqKey="Haddock E" first="Elaine" last="Haddock">Elaine Haddock</name></author><author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name></author><author><name sortKey="Ebihara, Hideki" sort="Ebihara, Hideki" uniqKey="Ebihara H" first="Hideki" last="Ebihara">Hideki Ebihara</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21334343</idno><idno type="pmid">21334343</idno><idno type="doi">10.1016/j.jim.2011.02.004</idno><idno type="wicri:Area/PubMed/Corpus">001546</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001546</idno><idno type="wicri:Area/PubMed/Curation">001546</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001546</idno><idno type="wicri:Area/PubMed/Checkpoint">001405</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001405</idno><idno type="wicri:Area/Ncbi/Merge">002297</idno><idno type="wicri:Area/Ncbi/Curation">002297</idno><idno type="wicri:Area/Ncbi/Checkpoint">002297</idno><idno type="wicri:Area/Main/Merge">002037</idno><idno type="wicri:Area/Main/Curation">002013</idno><idno type="wicri:Area/Main/Exploration">002013</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Validation of assays to monitor immune responses in the Syrian golden hamster (Mesocricetus auratus).</title><author><name sortKey="Zivcec, Marko" sort="Zivcec, Marko" uniqKey="Zivcec M" first="Marko" last="Zivcec">Marko Zivcec</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT</wicri:regionArea><placeName><region type="state">Montana</region></placeName></affiliation></author><author><name sortKey="Safronetz, David" sort="Safronetz, David" uniqKey="Safronetz D" first="David" last="Safronetz">David Safronetz</name></author><author><name sortKey="Haddock, Elaine" sort="Haddock, Elaine" uniqKey="Haddock E" first="Elaine" last="Haddock">Elaine Haddock</name></author><author><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name></author><author><name sortKey="Ebihara, Hideki" sort="Ebihara, Hideki" uniqKey="Ebihara H" first="Hideki" last="Ebihara">Hideki Ebihara</name></author></analytic><series><title level="j">Journal of immunological methods</title><idno type="eISSN">1872-7905</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Cricetinae</term><term>Immunity, Innate (genetics)</term><term>Immunity, Innate (immunology)</term><term>Lipopolysaccharides (pharmacology)</term><term>Mesocricetus (genetics)</term><term>Mesocricetus (immunology)</term><term>RNA (chemistry)</term><term>RNA (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>Reverse Transcriptase Polymerase Chain Reaction (standards)</term><term>Reverse Transcriptase Polymerase Chain Reaction (veterinary)</term><term>Vesicular stomatitis Indiana virus (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN ()</term><term>ARN (génétique)</term><term>Animaux</term><term>Cricetinae</term><term>Immunité innée (génétique)</term><term>Immunité innée (immunologie)</term><term>Lipopolysaccharides (pharmacologie)</term><term>Mesocricetus (génétique)</term><term>Mesocricetus (immunologie)</term><term>RT-PCR ()</term><term>RT-PCR (médecine vétérinaire)</term><term>RT-PCR (normes)</term><term>Virus de la stomatite vésiculeuse de type Indiana (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>RNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Lipopolysaccharides</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Immunity, Innate</term><term>Mesocricetus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN</term><term>Immunité innée</term><term>Mesocricetus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Immunité innée</term><term>Mesocricetus</term><term>Virus de la stomatite vésiculeuse de type Indiana</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Immunity, Innate</term><term>Mesocricetus</term><term>Vesicular stomatitis Indiana virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>RT-PCR</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>RT-PCR</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Lipopolysaccharides</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cricetinae</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN</term><term>Animaux</term><term>Cricetinae</term><term>RT-PCR</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Syrian golden hamster (Mesocricetus auratus) is a valuable but under-utilized animal model for studies of human viral pathogens such as bunyaviruses, arenaviruses, flaviviruses, henipaviruses, and SARS-coronavirus. A lack of suitable reagents and specific assays for monitoring host responses has limited the use of this animal model to clinical observations, pathology and humoral immune responses. The objective of this study was to establish and validate assays to monitor host immune responses in the hamster including important pro-inflammatory, anti-inflammatory and innate immune responses, as well as markers of apoptosis, cell proliferation, cell junction integrity and coagulation. Commercially available mouse and rat ELISA and luminex panels were screened for potential cross-reactivity, but were found to be of limited value for studying host responses in hamsters. Subsequently, quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays for the detection of 51 immune-related and four internal reference genes were developed. To validate the immune-related assays, hamsters were infected with vesicular stomatitis virus (VSV), Indiana species, or treated with lipopolysaccharide (LPS) and host immune responses were monitored in selected organs. Ribosomal protein L18 was identified as the most stable internal reference gene. In conclusion, these new assays will greatly improve the use of the hamster as an important small animal model in infectious disease research.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Montana</li></region></list><tree><noCountry><name sortKey="Ebihara, Hideki" sort="Ebihara, Hideki" uniqKey="Ebihara H" first="Hideki" last="Ebihara">Hideki Ebihara</name><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name><name sortKey="Haddock, Elaine" sort="Haddock, Elaine" uniqKey="Haddock E" first="Elaine" last="Haddock">Elaine Haddock</name><name sortKey="Safronetz, David" sort="Safronetz, David" uniqKey="Safronetz D" first="David" last="Safronetz">David Safronetz</name></noCountry><country name="États-Unis"><region name="Montana"><name sortKey="Zivcec, Marko" sort="Zivcec, Marko" uniqKey="Zivcec M" first="Marko" last="Zivcec">Marko Zivcec</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002013 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002013 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21334343
|texte= Validation of assays to monitor immune responses in the Syrian golden hamster (Mesocricetus auratus).
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21334343" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |