Validation of assays to monitor immune responses in the Syrian golden hamster (Mesocricetus auratus).
Identifieur interne : 001405 ( PubMed/Checkpoint ); précédent : 001404; suivant : 001406Validation of assays to monitor immune responses in the Syrian golden hamster (Mesocricetus auratus).
Auteurs : Marko Zivcec [États-Unis] ; David Safronetz ; Elaine Haddock ; Heinz Feldmann ; Hideki EbiharaSource :
- Journal of immunological methods [ 1872-7905 ] ; 2011.
Descripteurs français
- KwdFr :
- ARN (), ARN (génétique), Animaux, Cricetinae, Immunité innée (génétique), Immunité innée (immunologie), Lipopolysaccharides (pharmacologie), Mesocricetus (génétique), Mesocricetus (immunologie), RT-PCR (), RT-PCR (médecine vétérinaire), RT-PCR (normes), Virus de la stomatite vésiculeuse de type Indiana (immunologie).
- MESH :
- génétique : ARN, Immunité innée, Mesocricetus.
- immunologie : Immunité innée, Mesocricetus, Virus de la stomatite vésiculeuse de type Indiana.
- médecine vétérinaire : RT-PCR.
- normes : RT-PCR.
- pharmacologie : Lipopolysaccharides.
- ARN, Animaux, Cricetinae, RT-PCR.
English descriptors
- KwdEn :
- Animals, Cricetinae, Immunity, Innate (genetics), Immunity, Innate (immunology), Lipopolysaccharides (pharmacology), Mesocricetus (genetics), Mesocricetus (immunology), RNA (chemistry), RNA (genetics), Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (standards), Reverse Transcriptase Polymerase Chain Reaction (veterinary), Vesicular stomatitis Indiana virus (immunology).
- MESH :
- chemical , chemistry : RNA.
- chemical , genetics : RNA.
- chemical , pharmacology : Lipopolysaccharides.
- genetics : Immunity, Innate, Mesocricetus.
- immunology : Immunity, Innate, Mesocricetus, Vesicular stomatitis Indiana virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- standards : Reverse Transcriptase Polymerase Chain Reaction.
- veterinary : Reverse Transcriptase Polymerase Chain Reaction.
- Animals, Cricetinae.
Abstract
The Syrian golden hamster (Mesocricetus auratus) is a valuable but under-utilized animal model for studies of human viral pathogens such as bunyaviruses, arenaviruses, flaviviruses, henipaviruses, and SARS-coronavirus. A lack of suitable reagents and specific assays for monitoring host responses has limited the use of this animal model to clinical observations, pathology and humoral immune responses. The objective of this study was to establish and validate assays to monitor host immune responses in the hamster including important pro-inflammatory, anti-inflammatory and innate immune responses, as well as markers of apoptosis, cell proliferation, cell junction integrity and coagulation. Commercially available mouse and rat ELISA and luminex panels were screened for potential cross-reactivity, but were found to be of limited value for studying host responses in hamsters. Subsequently, quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays for the detection of 51 immune-related and four internal reference genes were developed. To validate the immune-related assays, hamsters were infected with vesicular stomatitis virus (VSV), Indiana species, or treated with lipopolysaccharide (LPS) and host immune responses were monitored in selected organs. Ribosomal protein L18 was identified as the most stable internal reference gene. In conclusion, these new assays will greatly improve the use of the hamster as an important small animal model in infectious disease research.
DOI: 10.1016/j.jim.2011.02.004
PubMed: 21334343
Affiliations:
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qualifier="chemistry" xml:lang="en"><term>RNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Lipopolysaccharides</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Immunity, Innate</term><term>Mesocricetus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN</term><term>Immunité innée</term><term>Mesocricetus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Immunité innée</term><term>Mesocricetus</term><term>Virus de la stomatite vésiculeuse de type Indiana</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Immunity, Innate</term><term>Mesocricetus</term><term>Vesicular stomatitis Indiana virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>RT-PCR</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>RT-PCR</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Lipopolysaccharides</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cricetinae</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN</term><term>Animaux</term><term>Cricetinae</term><term>RT-PCR</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The Syrian golden hamster (Mesocricetus auratus) is a valuable but under-utilized animal model for studies of human viral pathogens such as bunyaviruses, arenaviruses, flaviviruses, henipaviruses, and SARS-coronavirus. A lack of suitable reagents and specific assays for monitoring host responses has limited the use of this animal model to clinical observations, pathology and humoral immune responses. The objective of this study was to establish and validate assays to monitor host immune responses in the hamster including important pro-inflammatory, anti-inflammatory and innate immune responses, as well as markers of apoptosis, cell proliferation, cell junction integrity and coagulation. Commercially available mouse and rat ELISA and luminex panels were screened for potential cross-reactivity, but were found to be of limited value for studying host responses in hamsters. Subsequently, quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays for the detection of 51 immune-related and four internal reference genes were developed. To validate the immune-related assays, hamsters were infected with vesicular stomatitis virus (VSV), Indiana species, or treated with lipopolysaccharide (LPS) and host immune responses were monitored in selected organs. Ribosomal protein L18 was identified as the most stable internal reference gene. In conclusion, these new assays will greatly improve the use of the hamster as an important small animal model in infectious disease research.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21334343</PMID><DateCompleted><Year>2011</Year><Month>07</Month><Day>05</Day></DateCompleted><DateRevised><Year>2020</Year><Month>04</Month><Day>07</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-7905</ISSN><JournalIssue CitedMedium="Internet"><Volume>368</Volume><Issue>1-2</Issue><PubDate><Year>2011</Year><Month>May</Month><Day>31</Day></PubDate></JournalIssue><Title>Journal of immunological methods</Title><ISOAbbreviation>J. Immunol. 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The objective of this study was to establish and validate assays to monitor host immune responses in the hamster including important pro-inflammatory, anti-inflammatory and innate immune responses, as well as markers of apoptosis, cell proliferation, cell junction integrity and coagulation. Commercially available mouse and rat ELISA and luminex panels were screened for potential cross-reactivity, but were found to be of limited value for studying host responses in hamsters. Subsequently, quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) assays for the detection of 51 immune-related and four internal reference genes were developed. To validate the immune-related assays, hamsters were infected with vesicular stomatitis virus (VSV), Indiana species, or treated with lipopolysaccharide (LPS) and host immune responses were monitored in selected organs. Ribosomal protein L18 was identified as the most stable internal reference gene. 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IdType="mid">NIHMS275579</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Am J Trop Med Hyg. 2003 Sep;69(3):269-76</Citation><ArticleIdList><ArticleId IdType="pubmed">14628943</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Am J Trop Med Hyg. 1980 Sep;29(5):779-84</Citation><ArticleIdList><ArticleId IdType="pubmed">6254382</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Biotechniques. 2004 Jul;37(1):112-4, 116, 118-9</Citation><ArticleIdList><ArticleId IdType="pubmed">15283208</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect Dis. 2001 May 15;183(10):1431-6</Citation><ArticleIdList><ArticleId IdType="pubmed">11319679</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Gen Virol. 1996 Aug;77 ( Pt 8):1925-34</Citation><ArticleIdList><ArticleId IdType="pubmed">8760444</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Acta Pathol Microbiol Immunol Scand C. 1984 Aug;92(4):197-200</Citation><ArticleIdList><ArticleId IdType="pubmed">6507105</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Endocr Rev. 1997 Feb;18(1):4-25</Citation><ArticleIdList><ArticleId IdType="pubmed">9034784</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect Dis. 2004 Jun 1;189(11):2072-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15143475</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Vet Pathol. 1991 Sep;28(5):410-8</Citation><ArticleIdList><ArticleId IdType="pubmed">1750167</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Infect Immun. 1980 Nov;30(2):397-401</Citation><ArticleIdList><ArticleId IdType="pubmed">6254888</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Cancer Gene Ther. 2009 Dec;16(12):912-22</Citation><ArticleIdList><ArticleId IdType="pubmed">19478829</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Br J Exp Pathol. 1972 Feb;53(1):59-77</Citation><ArticleIdList><ArticleId IdType="pubmed">5014245</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Parasitol. 2006 Oct;92(5):1104-7</Citation><ArticleIdList><ArticleId IdType="pubmed">17152960</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Infect Immun. 2006 Jul;74(7):4172-9</Citation><ArticleIdList><ArticleId IdType="pubmed">16790792</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Infect Dis. 2002 Nov 15;186(10):1390-5</Citation><ArticleIdList><ArticleId IdType="pubmed">12404153</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2004 May;78(10):5458-65</Citation><ArticleIdList><ArticleId IdType="pubmed">15113924</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Am J Trop Med Hyg. 2006 Jun;74(6):1096-102</Citation><ArticleIdList><ArticleId IdType="pubmed">16760527</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>BMC Genomics. 2007 Jun 25;8:190</Citation><ArticleIdList><ArticleId IdType="pubmed">17592630</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Virology. 2001 Oct 10;289(1):6-14</Citation><ArticleIdList><ArticleId IdType="pubmed">11601912</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2001 Apr 24;98(9):5093-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11309484</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Crit Care. 1999;3(1):11-16</Citation><ArticleIdList><ArticleId IdType="pubmed">11056717</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Clin Endocrinol Metab. 2001 Jan;86(1):396-404</Citation><ArticleIdList><ArticleId IdType="pubmed">11232031</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 1999 Aug;73(8):6245-50</Citation><ArticleIdList><ArticleId IdType="pubmed">10400714</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Immunol. 2001 Feb 1;166(3):1912-20</Citation><ArticleIdList><ArticleId IdType="pubmed">11160239</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>BMC Musculoskelet Disord. 2010 Jun 29;11:136</Citation><ArticleIdList><ArticleId IdType="pubmed">20587038</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Infect Immun. 1981 Nov;34(2):540-9</Citation><ArticleIdList><ArticleId IdType="pubmed">6273320</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>BMC Genomics. 2010 Apr 19;11:251</Citation><ArticleIdList><ArticleId IdType="pubmed">20403183</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Emerg Infect Dis. 2001 Jul-Aug;7(4):714-21</Citation><ArticleIdList><ArticleId IdType="pubmed">11585537</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Infect Immun. 1981 Jun;32(3):1007-13</Citation><ArticleIdList><ArticleId IdType="pubmed">6265369</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Curr Protoc Microbiol. 2006 Sep;Chapter 12:Unit 12E.2</Citation><ArticleIdList><ArticleId IdType="pubmed">18770576</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Antiviral Res. 1993 Jan;20(1):57-70</Citation><ArticleIdList><ArticleId IdType="pubmed">8384433</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>J Virol. 2005 Jan;79(1):503-11</Citation><ArticleIdList><ArticleId IdType="pubmed">15596843</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Am J Pathol. 2003 Nov;163(5):2127-37</Citation><ArticleIdList><ArticleId IdType="pubmed">14578210</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 1995 May 9;92(10):4477-81</Citation><ArticleIdList><ArticleId IdType="pubmed">7753828</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Am J Trop Med Hyg. 2007 Feb;76(2):299-306</Citation><ArticleIdList><ArticleId IdType="pubmed">17297039</ArticleId></ArticleIdList></Reference></ReferenceList><ReferenceList><Reference><Citation>Infect Immun. 1998 May;66(5):2135-42</Citation><ArticleIdList><ArticleId IdType="pubmed">9573100</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Montana</li></region></list><tree><noCountry><name sortKey="Ebihara, Hideki" sort="Ebihara, Hideki" uniqKey="Ebihara H" first="Hideki" last="Ebihara">Hideki Ebihara</name><name sortKey="Feldmann, Heinz" sort="Feldmann, Heinz" uniqKey="Feldmann H" first="Heinz" last="Feldmann">Heinz Feldmann</name><name sortKey="Haddock, Elaine" sort="Haddock, Elaine" uniqKey="Haddock E" first="Elaine" last="Haddock">Elaine Haddock</name><name sortKey="Safronetz, David" sort="Safronetz, David" uniqKey="Safronetz D" first="David" last="Safronetz">David Safronetz</name></noCountry><country name="États-Unis"><region name="Montana"><name sortKey="Zivcec, Marko" sort="Zivcec, Marko" uniqKey="Zivcec M" first="Marko" last="Zivcec">Marko Zivcec</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |