Severe acute respiratory syndrome coronavirus elicits a weak interferon response compared to traditional interferon-inducing viruses.
Identifieur interne : 003021 ( Main/Exploration ); précédent : 003020; suivant : 003022Severe acute respiratory syndrome coronavirus elicits a weak interferon response compared to traditional interferon-inducing viruses.
Auteurs : Carolina Scagnolari [Italie] ; Simona Trombetti ; Simona Cicetti ; Silvia Antonelli ; Carla Selvaggi ; Lorena Perrone ; Michela Visca ; Sara Romano ; Guido AntonelliSource :
- Intervirology [ 1423-0100 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : Interférons.
- immunologie : Agranulocytes, Interférons, Vesiculovirus, Virus de la maladie de Newcastle, Virus du SRAS.
- virologie : Agranulocytes.
- Analyse de profil d'expression de gènes, Cellules cultivées, Humains.
English descriptors
- KwdEn :
- MESH :
- chemical , biosynthesis : Interferons.
- chemical , immunology : Interferons.
- immunology : Leukocytes, Mononuclear, Newcastle disease virus, SARS Virus, Vesiculovirus.
- virology : Leukocytes, Mononuclear.
- Cells, Cultured, Gene Expression Profiling, Humans.
Abstract
The aim of the present study is to investigate changes of interferon (IFN) production occurring over the first 48 h after infection of peripheral blood mononuclear cells (PBMCs) with severe acute respiratory syndrome (SARS) coronavirus (CoV) and to compare these changes to those induced by well-established IFN-inducing viruses, such as vesicular stomatitis (VSV) and Newcastle viruses (NDV). Experiments have been carried out using PBMCs of 10 different healthy donors. The results showed that the antiviral activity of IFN contained in the supernatant of SARS-CoV-infected PBMCs was lower than those induced by VSV and NDV. Consequently, SARS-CoV induces a lower synthesis of IFN-alpha, -beta and -gamma compared to VSV and NDV. Characterization of the profile of IFN-alpha subtypes genes expression in SARS-CoV-infected PBMCs demonstrated that the level of IFN-alpha2 and -6 subtypes were higher compared to other IFN-alpha subtypes namely, IFN-alpha5, -8, -10, -13/1, -17, and -21. In conclusion, SARS-CoV induces IFNs to a less extent compared to VSV and NDV, thus suggesting that the IFN system does play a limited role in early host defense against SARS-CoV infection.
DOI: 10.1159/000154258
PubMed: 18781076
Affiliations:
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Virology Section, Sapienza University of Rome, Rome, Italy. carolina.scagnolari@uniroma1.it</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Experimental Medicine - Virology Section, Sapienza University of Rome, Rome</wicri:regionArea><placeName><settlement type="city">Rome</settlement><region nuts="2">Latium</region></placeName></affiliation></author><author><name sortKey="Trombetti, Simona" sort="Trombetti, Simona" uniqKey="Trombetti S" first="Simona" last="Trombetti">Simona Trombetti</name></author><author><name sortKey="Cicetti, Simona" sort="Cicetti, Simona" uniqKey="Cicetti S" first="Simona" last="Cicetti">Simona Cicetti</name></author><author><name sortKey="Antonelli, Silvia" sort="Antonelli, Silvia" uniqKey="Antonelli S" first="Silvia" last="Antonelli">Silvia Antonelli</name></author><author><name sortKey="Selvaggi, Carla" sort="Selvaggi, Carla" uniqKey="Selvaggi C" first="Carla" last="Selvaggi">Carla Selvaggi</name></author><author><name sortKey="Perrone, Lorena" sort="Perrone, Lorena" uniqKey="Perrone L" first="Lorena" last="Perrone">Lorena Perrone</name></author><author><name sortKey="Visca, Michela" sort="Visca, Michela" uniqKey="Visca M" first="Michela" last="Visca">Michela Visca</name></author><author><name sortKey="Romano, Sara" sort="Romano, Sara" uniqKey="Romano S" first="Sara" last="Romano">Sara Romano</name></author><author><name sortKey="Antonelli, Guido" sort="Antonelli, Guido" uniqKey="Antonelli G" first="Guido" last="Antonelli">Guido Antonelli</name></author></analytic><series><title level="j">Intervirology</title><idno type="eISSN">1423-0100</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cells, Cultured</term><term>Gene Expression Profiling</term><term>Humans</term><term>Interferons (biosynthesis)</term><term>Interferons (immunology)</term><term>Leukocytes, Mononuclear (immunology)</term><term>Leukocytes, Mononuclear (virology)</term><term>Newcastle disease virus (immunology)</term><term>SARS Virus (immunology)</term><term>Vesiculovirus (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agranulocytes (immunologie)</term><term>Agranulocytes (virologie)</term><term>Analyse de profil d'expression de gènes</term><term>Cellules cultivées</term><term>Humains</term><term>Interférons (biosynthèse)</term><term>Interférons (immunologie)</term><term>Vesiculovirus (immunologie)</term><term>Virus de la maladie de Newcastle (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Interferons</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Interferons</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Interférons</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Agranulocytes</term><term>Interférons</term><term>Vesiculovirus</term><term>Virus de la maladie de Newcastle</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Leukocytes, Mononuclear</term><term>Newcastle disease virus</term><term>SARS Virus</term><term>Vesiculovirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Agranulocytes</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Leukocytes, Mononuclear</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>Gene Expression Profiling</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term><term>Cellules cultivées</term><term>Humains</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of the present study is to investigate changes of interferon (IFN) production occurring over the first 48 h after infection of peripheral blood mononuclear cells (PBMCs) with severe acute respiratory syndrome (SARS) coronavirus (CoV) and to compare these changes to those induced by well-established IFN-inducing viruses, such as vesicular stomatitis (VSV) and Newcastle viruses (NDV). Experiments have been carried out using PBMCs of 10 different healthy donors. The results showed that the antiviral activity of IFN contained in the supernatant of SARS-CoV-infected PBMCs was lower than those induced by VSV and NDV. Consequently, SARS-CoV induces a lower synthesis of IFN-alpha, -beta and -gamma compared to VSV and NDV. Characterization of the profile of IFN-alpha subtypes genes expression in SARS-CoV-infected PBMCs demonstrated that the level of IFN-alpha2 and -6 subtypes were higher compared to other IFN-alpha subtypes namely, IFN-alpha5, -8, -10, -13/1, -17, and -21. In conclusion, SARS-CoV induces IFNs to a less extent compared to VSV and NDV, thus suggesting that the IFN system does play a limited role in early host defense against SARS-CoV infection.</div></front></TEI><affiliations><list><country><li>Italie</li></country><region><li>Latium</li></region><settlement><li>Rome</li></settlement></list><tree><noCountry><name sortKey="Antonelli, Guido" sort="Antonelli, Guido" uniqKey="Antonelli G" first="Guido" last="Antonelli">Guido Antonelli</name><name sortKey="Antonelli, Silvia" sort="Antonelli, Silvia" uniqKey="Antonelli S" first="Silvia" last="Antonelli">Silvia Antonelli</name><name sortKey="Cicetti, Simona" sort="Cicetti, Simona" uniqKey="Cicetti S" first="Simona" last="Cicetti">Simona Cicetti</name><name sortKey="Perrone, Lorena" sort="Perrone, Lorena" uniqKey="Perrone L" first="Lorena" last="Perrone">Lorena Perrone</name><name sortKey="Romano, Sara" sort="Romano, Sara" uniqKey="Romano S" first="Sara" last="Romano">Sara Romano</name><name sortKey="Selvaggi, Carla" sort="Selvaggi, Carla" uniqKey="Selvaggi C" first="Carla" last="Selvaggi">Carla Selvaggi</name><name sortKey="Trombetti, Simona" sort="Trombetti, Simona" uniqKey="Trombetti S" first="Simona" last="Trombetti">Simona Trombetti</name><name sortKey="Visca, Michela" sort="Visca, Michela" uniqKey="Visca M" first="Michela" last="Visca">Michela Visca</name></noCountry><country name="Italie"><region name="Latium"><name sortKey="Scagnolari, Carolina" sort="Scagnolari, Carolina" uniqKey="Scagnolari C" first="Carolina" last="Scagnolari">Carolina Scagnolari</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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