The 3a Accessory Protein of SARS Coronavirus Specifically Interacts with the 5‘UTR of Its Genomic RNA, Using a Unique 75 Amino Acid Interaction Domain†
Identifieur interne : 003795 ( Main/Exploration ); précédent : 003794; suivant : 003796The 3a Accessory Protein of SARS Coronavirus Specifically Interacts with the 5‘UTR of Its Genomic RNA, Using a Unique 75 Amino Acid Interaction Domain†
Auteurs : Kulbhushan Sharma [Singapour] ; Milan Surjit [Singapour] ; Namita Satija [Singapour] ; Boping Liu [Singapour] ; Vincent T. K. Chow [Singapour] ; Sunil K. Lal [Singapour, Inde]Source :
- Biochemistry [ 0006-2960 ] ; 2007.
Descripteurs français
- KwdFr :
- ARN viral (), ARN viral (métabolisme), Délétion de gène, Génome, Liaison aux protéines, Motifs d'acides aminés, Protéines de liaison à l'ARN (), Protéines de liaison à l'ARN (génétique), Protéines de liaison à l'ARN (métabolisme), Protéines nucléocapside (), Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Rayons ultraviolets, Régions non traduites (), Régions non traduites (génétique), Régions non traduites (métabolisme), Régulation de l'expression des gènes viraux, Saccharomyces cerevisiae (), Saccharomyces cerevisiae (génétique), Saccharomyces cerevisiae (métabolisme), Séquence d'acides aminés, Techniques de double hybride, Test de retard de migration électrophorétique, Virus du SRAS (), Virus du SRAS (génétique).
- MESH :
- génétique : Protéines de liaison à l'ARN, Protéines nucléocapside, Régions non traduites, Saccharomyces cerevisiae, Virus du SRAS.
- métabolisme : ARN viral, Protéines de liaison à l'ARN, Protéines nucléocapside, Régions non traduites, Saccharomyces cerevisiae.
- ARN viral, Délétion de gène, Génome, Liaison aux protéines, Motifs d'acides aminés, Protéines de liaison à l'ARN, Protéines nucléocapside, Rayons ultraviolets, Régions non traduites, Régulation de l'expression des gènes viraux, Saccharomyces cerevisiae, Séquence d'acides aminés, Techniques de double hybride, Test de retard de migration électrophorétique, Virus du SRAS.
English descriptors
- KwdEn :
- Amino Acid Motifs, Amino Acid Sequence, Electrophoretic Mobility Shift Assay, Gene Deletion, Gene Expression Regulation, Viral, Genome, Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), Protein Binding, RNA, Viral (chemistry), RNA, Viral (metabolism), RNA-Binding Proteins (chemistry), RNA-Binding Proteins (genetics), RNA-Binding Proteins (metabolism), SARS Virus (chemistry), SARS Virus (genetics), Saccharomyces cerevisiae (chemistry), Saccharomyces cerevisiae (genetics), Saccharomyces cerevisiae (metabolism), Two-Hybrid System Techniques, Ultraviolet Rays, Untranslated Regions (chemistry), Untranslated Regions (genetics), Untranslated Regions (metabolism).
- MESH :
- chemical , chemistry : Nucleocapsid Proteins, RNA, Viral, RNA-Binding Proteins, Untranslated Regions.
- chemical , genetics : Nucleocapsid Proteins, RNA-Binding Proteins, Untranslated Regions.
- chemical , metabolism : Nucleocapsid Proteins, RNA, Viral, RNA-Binding Proteins, Untranslated Regions.
- chemistry : SARS Virus, Saccharomyces cerevisiae.
- genetics : SARS Virus, Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- Amino Acid Motifs, Amino Acid Sequence, Electrophoretic Mobility Shift Assay, Gene Deletion, Gene Expression Regulation, Viral, Genome, Protein Binding, Two-Hybrid System Techniques, Ultraviolet Rays.
Abstract
More than four years have passed since the outbreak of the severe acute respiratory syndrome (SARS) epidemic, and still very little is known about the molecular biology and pathogenesis of this deadly virus. Among the accessory proteins of the SARS coronavirus (SARS-CoV), the 3a protein has been shown to interact with the spike, envelope, and membrane glycoprotein and has recently been established to be a structural component of capsid. Recent studies suggest that the 3a protein may function as an ion channel and may promote virus release. In order to further characterize the functional properties of this protein, we initiated studies to check its RNA binding activity. Using the yeast three-hybrid system, electrophoretic mobility shift assay (EMSA), and ultraviolet (UV) cross-linking techniques, we have shown that the 3a protein is capable of binding specifically to the 5‘ untranslated region (5‘UTR) of the SARS virus genomic RNA. Further, we have mapped the interaction domain of the 3a protein responsible for this RNA−protein interaction using a series of deletion mutants and defined it to the central 75 amino acid region. This RNA binding motif of 3a does not share homology with any other known RNA binding protein and may have an important role in viral capsid assembly and pathogenesis.
Url:
DOI: 10.1021/bi062057p
Affiliations:
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cerevisiae (metabolism)</term><term>Two-Hybrid System Techniques</term><term>Ultraviolet Rays</term><term>Untranslated Regions (chemistry)</term><term>Untranslated Regions (genetics)</term><term>Untranslated Regions (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral ()</term><term>ARN viral (métabolisme)</term><term>Délétion de gène</term><term>Génome</term><term>Liaison aux protéines</term><term>Motifs d'acides aminés</term><term>Protéines de liaison à l'ARN ()</term><term>Protéines de liaison à l'ARN (génétique)</term><term>Protéines de liaison à l'ARN (métabolisme)</term><term>Protéines nucléocapside ()</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (métabolisme)</term><term>Rayons ultraviolets</term><term>Régions non traduites ()</term><term>Régions non traduites (génétique)</term><term>Régions non traduites (métabolisme)</term><term>Régulation de l'expression des gènes viraux</term><term>Saccharomyces cerevisiae ()</term><term>Saccharomyces cerevisiae (génétique)</term><term>Saccharomyces cerevisiae (métabolisme)</term><term>Séquence d'acides aminés</term><term>Techniques de double hybride</term><term>Test de retard de migration électrophorétique</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Nucleocapsid Proteins</term><term>RNA, Viral</term><term>RNA-Binding Proteins</term><term>Untranslated Regions</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>RNA-Binding Proteins</term><term>Untranslated Regions</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term><term>RNA, Viral</term><term>RNA-Binding Proteins</term><term>Untranslated Regions</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de liaison à l'ARN</term><term>Protéines nucléocapside</term><term>Régions non traduites</term><term>Saccharomyces cerevisiae</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN viral</term><term>Protéines de liaison à l'ARN</term><term>Protéines nucléocapside</term><term>Régions non traduites</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Motifs</term><term>Amino Acid Sequence</term><term>Electrophoretic Mobility Shift Assay</term><term>Gene Deletion</term><term>Gene Expression Regulation, Viral</term><term>Genome</term><term>Protein Binding</term><term>Two-Hybrid System Techniques</term><term>Ultraviolet Rays</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN viral</term><term>Délétion de gène</term><term>Génome</term><term>Liaison aux protéines</term><term>Motifs d'acides aminés</term><term>Protéines de liaison à l'ARN</term><term>Protéines nucléocapside</term><term>Rayons ultraviolets</term><term>Régions non traduites</term><term>Régulation de l'expression des gènes viraux</term><term>Saccharomyces cerevisiae</term><term>Séquence d'acides aminés</term><term>Techniques de double hybride</term><term>Test de retard de migration électrophorétique</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract">More than four years have passed since the outbreak of the severe acute respiratory syndrome (SARS) epidemic, and still very little is known about the molecular biology and pathogenesis of this deadly virus. Among the accessory proteins of the SARS coronavirus (SARS-CoV), the 3a protein has been shown to interact with the spike, envelope, and membrane glycoprotein and has recently been established to be a structural component of capsid. Recent studies suggest that the 3a protein may function as an ion channel and may promote virus release. In order to further characterize the functional properties of this protein, we initiated studies to check its RNA binding activity. Using the yeast three-hybrid system, electrophoretic mobility shift assay (EMSA), and ultraviolet (UV) cross-linking techniques, we have shown that the 3a protein is capable of binding specifically to the 5‘ untranslated region (5‘UTR) of the SARS virus genomic RNA. Further, we have mapped the interaction domain of the 3a protein responsible for this RNA−protein interaction using a series of deletion mutants and defined it to the central 75 amino acid region. This RNA binding motif of 3a does not share homology with any other known RNA binding protein and may have an important role in viral capsid assembly and pathogenesis.</div></front></TEI><affiliations><list><country><li>Inde</li><li>Singapour</li></country><orgName><li>Université nationale de Singapour</li></orgName></list><tree><country name="Singapour"><noRegion><name sortKey="Sharma, Kulbhushan" sort="Sharma, Kulbhushan" uniqKey="Sharma K" first="Kulbhushan" last="Sharma">Kulbhushan Sharma</name></noRegion><name sortKey="Chow, Vincent T K" sort="Chow, Vincent T K" uniqKey="Chow V" first="Vincent T. K." last="Chow">Vincent T. K. Chow</name><name sortKey="Lal, Sunil K" sort="Lal, Sunil K" uniqKey="Lal S" first="Sunil K." last="Lal">Sunil K. Lal</name><name sortKey="Liu, Boping" sort="Liu, Boping" uniqKey="Liu B" first="Boping" last="Liu">Boping Liu</name><name sortKey="Satija, Namita" sort="Satija, Namita" uniqKey="Satija N" first="Namita" last="Satija">Namita Satija</name><name sortKey="Surjit, Milan" sort="Surjit, Milan" uniqKey="Surjit M" first="Milan" last="Surjit">Milan Surjit</name></country><country name="Inde"><noRegion><name sortKey="Lal, Sunil K" sort="Lal, Sunil K" uniqKey="Lal S" first="Sunil K." last="Lal">Sunil K. Lal</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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