The 3a Accessory Protein of SARS Coronavirus Specifically Interacts with the 5‘UTR of Its Genomic RNA, Using a Unique 75 Amino Acid Interaction Domain†
Identifieur interne : 000034 ( Istex/Curation ); précédent : 000033; suivant : 000035The 3a Accessory Protein of SARS Coronavirus Specifically Interacts with the 5‘UTR of Its Genomic RNA, Using a Unique 75 Amino Acid Interaction Domain†
Auteurs : Kulbhushan Sharma [Singapour] ; Milan Surjit [Singapour] ; Namita Satija [Singapour] ; Boping Liu [Singapour] ; Vincent T. K. Chow [Singapour] ; Sunil K. Lal [Singapour, Inde]Source :
- Biochemistry [ 0006-2960 ] ; 2007.
Abstract
More than four years have passed since the outbreak of the severe acute respiratory syndrome (SARS) epidemic, and still very little is known about the molecular biology and pathogenesis of this deadly virus. Among the accessory proteins of the SARS coronavirus (SARS-CoV), the 3a protein has been shown to interact with the spike, envelope, and membrane glycoprotein and has recently been established to be a structural component of capsid. Recent studies suggest that the 3a protein may function as an ion channel and may promote virus release. In order to further characterize the functional properties of this protein, we initiated studies to check its RNA binding activity. Using the yeast three-hybrid system, electrophoretic mobility shift assay (EMSA), and ultraviolet (UV) cross-linking techniques, we have shown that the 3a protein is capable of binding specifically to the 5‘ untranslated region (5‘UTR) of the SARS virus genomic RNA. Further, we have mapped the interaction domain of the 3a protein responsible for this RNA−protein interaction using a series of deletion mutants and defined it to the central 75 amino acid region. This RNA binding motif of 3a does not share homology with any other known RNA binding protein and may have an important role in viral capsid assembly and pathogenesis.
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DOI: 10.1021/bi062057p
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5‘UTR of Its Genomic RNA, Using a Unique 75 Amino Acid Interaction Domain<ref type="bib" target="#bi062057pAF2">†</ref></title><author><name sortKey="Sharma, Kulbhushan" sort="Sharma, Kulbhushan" uniqKey="Sharma K" first="Kulbhushan" last="Sharma">Kulbhushan Sharma</name><affiliation wicri:level="1"><mods:affiliation>Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Road, New Delhi 110067,India, and Microbiology Department, National University of Singapore, Singapore</mods:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Road, New Delhi 110067,India, and Microbiology Department, National University of Singapore</wicri:regionArea></affiliation><affiliation><mods:affiliation> International Centre for Genetic Engineering & Biotechnology.</mods:affiliation></affiliation></author><author><name sortKey="Surjit, Milan" sort="Surjit, Milan" uniqKey="Surjit M" first="Milan" last="Surjit">Milan Surjit</name><affiliation wicri:level="1"><mods:affiliation>Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Road, New Delhi 110067,India, and Microbiology Department, National University of Singapore, Singapore</mods:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Road, New Delhi 110067,India, and Microbiology Department, National University of Singapore</wicri:regionArea></affiliation><affiliation><mods:affiliation> International Centre for Genetic Engineering & Biotechnology.</mods:affiliation></affiliation></author><author><name sortKey="Satija, Namita" sort="Satija, Namita" uniqKey="Satija N" first="Namita" last="Satija">Namita Satija</name><affiliation wicri:level="1"><mods:affiliation>Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Road, New Delhi 110067,India, and Microbiology Department, National University of Singapore, Singapore</mods:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Road, New Delhi 110067,India, and Microbiology Department, National University of Singapore</wicri:regionArea></affiliation><affiliation><mods:affiliation> International Centre for Genetic Engineering & Biotechnology.</mods:affiliation></affiliation></author><author><name sortKey="Liu, Boping" sort="Liu, Boping" uniqKey="Liu B" first="Boping" last="Liu">Boping Liu</name><affiliation wicri:level="1"><mods:affiliation>Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Road, New Delhi 110067,India, and Microbiology Department, National University of Singapore, Singapore</mods:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Road, New Delhi 110067,India, and Microbiology Department, National University of Singapore</wicri:regionArea></affiliation><affiliation><mods:affiliation> National University of Singapore.</mods:affiliation></affiliation></author><author><name sortKey="Chow, Vincent T K" sort="Chow, Vincent T K" uniqKey="Chow V" first="Vincent T. K." last="Chow">Vincent T. K. Chow</name><affiliation wicri:level="1"><mods:affiliation>Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Road, New Delhi 110067,India, and Microbiology Department, National University of Singapore, Singapore</mods:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Road, New Delhi 110067,India, and Microbiology Department, National University of Singapore</wicri:regionArea></affiliation><affiliation><mods:affiliation> National University of Singapore.</mods:affiliation></affiliation></author><author><name sortKey="Lal, Sunil K" sort="Lal, Sunil K" uniqKey="Lal S" first="Sunil K." last="Lal">Sunil K. Lal</name><affiliation wicri:level="1"><mods:affiliation>Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Road, New Delhi 110067,India, and Microbiology Department, National University of Singapore, Singapore</mods:affiliation><country xml:lang="fr">Singapour</country><wicri:regionArea>Virology Group, International Centre for Genetic Engineering & Biotechnology, Aruna Asaf Ali Road, New Delhi 110067,India, and Microbiology Department, National University of Singapore</wicri:regionArea></affiliation><affiliation><mods:affiliation> International Centre for Genetic Engineering & Biotechnology.</mods:affiliation></affiliation><affiliation wicri:level="1"><mods:affiliation> Corresponding author. E-mail address: sunillal@icgeb.res.in.</mods:affiliation><country wicri:rule="url">Inde</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Biochemistry</title><title level="j" type="abbrev">Biochemistry</title><idno type="ISSN">0006-2960</idno><idno type="eISSN">1520-4995</idno><imprint><publisher>American Chemical Society</publisher><date type="e-published">2007</date><date type="published">2007</date><biblScope unit="vol">46</biblScope><biblScope unit="issue">22</biblScope><biblScope unit="page" from="6488">6488</biblScope><biblScope unit="page" to="6499">6499</biblScope></imprint><idno type="ISSN">0006-2960</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-2960</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">More than four years have passed since the outbreak of the severe acute respiratory syndrome (SARS) epidemic, and still very little is known about the molecular biology and pathogenesis of this deadly virus. Among the accessory proteins of the SARS coronavirus (SARS-CoV), the 3a protein has been shown to interact with the spike, envelope, and membrane glycoprotein and has recently been established to be a structural component of capsid. Recent studies suggest that the 3a protein may function as an ion channel and may promote virus release. In order to further characterize the functional properties of this protein, we initiated studies to check its RNA binding activity. Using the yeast three-hybrid system, electrophoretic mobility shift assay (EMSA), and ultraviolet (UV) cross-linking techniques, we have shown that the 3a protein is capable of binding specifically to the 5‘ untranslated region (5‘UTR) of the SARS virus genomic RNA. Further, we have mapped the interaction domain of the 3a protein responsible for this RNA−protein interaction using a series of deletion mutants and defined it to the central 75 amino acid region. This RNA binding motif of 3a does not share homology with any other known RNA binding protein and may have an important role in viral capsid assembly and pathogenesis.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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