Interaction of severe acute respiratory syndrome- associated coronavirus with dendritic cells
Identifieur interne : 004436 ( Main/Exploration ); précédent : 004435; suivant : 004437Interaction of severe acute respiratory syndrome- associated coronavirus with dendritic cells
Auteurs : Martin Spiegel [Allemagne] ; Kerstin Schneider [Allemagne] ; Friedemann Weber [Allemagne] ; Manfred Weidmann [Allemagne] ; Frank T. Hufert [Allemagne]Source :
- Journal of general virology [ 0022-1317 ] ; 2006.
Descripteurs français
- KwdFr :
- ADN viral (génétique), Antigènes de surface (métabolisme), Apoptose, Cellules dendritiques (cytologie), Cellules dendritiques (immunologie), Cellules dendritiques (virologie), Différenciation cellulaire, Humains, Immunité innée, Interféron alpha (biosynthèse), Phénotype, Réplication virale, Séquence nucléotidique, Techniques in vitro, Virus du SRAS (génétique), Virus du SRAS (pathogénicité), Virus du SRAS (physiologie).
- MESH :
- biosynthèse : Interféron alpha.
- cytologie : Cellules dendritiques.
- génétique : ADN viral, Virus du SRAS.
- immunologie : Cellules dendritiques.
- métabolisme : Antigènes de surface.
- pathogénicité : Virus du SRAS.
- physiologie : Virus du SRAS.
- virologie : Cellules dendritiques.
- Pascal (Inist)
English descriptors
- KwdEn :
- Antigens, Surface (metabolism), Apoptosis, Base Sequence, Cell Differentiation, Coronavirus, DNA, Viral (genetics), Dendritic Cells (cytology), Dendritic Cells (immunology), Dendritic Cells (virology), Dendritic cell, Humans, Immunity, Innate, In Vitro Techniques, Interferon-alpha (biosynthesis), Microbiology, Phenotype, SARS Virus (genetics), SARS Virus (pathogenicity), SARS Virus (physiology), Severe acute respiratory syndrome, Virology, Virus Replication.
- MESH :
- chemical , biosynthesis : Interferon-alpha.
- chemical , genetics : DNA, Viral.
- chemical , metabolism : Antigens, Surface.
- cytology : Dendritic Cells.
- genetics : SARS Virus.
- immunology : Dendritic Cells.
- pathogenicity : SARS Virus.
- physiology : SARS Virus.
- virology : Dendritic Cells.
- Apoptosis, Base Sequence, Cell Differentiation, Humans, Immunity, Innate, In Vitro Techniques, Phenotype, Virus Replication.
Abstract
Severe acute respiratory syndrome (SARS) of humans is caused by a novel coronavirus of zoonotic origin termed SARS-associated coronavirus (SARS-CoV). The virus induces severe injury of lung tissue, as well as lymphopenia and destruction of the architecture of lymphatic tissue by as-yet-unknown mechanisms. In this study, the interaction of SARS-CoV with dendritic cells (DCs), the key regulators of immune responses, was analysed. Monocyte-derived DCs were infected with SARS-CoV and analysed for viability, surface-marker expression and alpha interferon (IFN-α) induction. SARS-CoV infection was monitored by quantitative RT-PCR, immunofluorescence analysis and recovery experiments. SARS-CoV infected both immature and mature DCs, although replication efficiency was low. Immature DCs were activated by SARS-CoV infection and by UV-inactivated SARS-CoV. Infected DCs were still viable on day 6 post-infection, but major histocompatibility complex class I upregulation was missing, indicating that DC function was impaired. Additionally, SARS-CoV infection induced a delayed activation of IFN-a expression. Therefore, it is concluded that SARS-CoV has the ability to circumvent both the innate and the adaptive immune systems.
Affiliations:
- Allemagne
- Bade-Wurtemberg, Basse-Saxe, District de Fribourg-en-Brisgau
- Fribourg-en-Brisgau, Göttingen
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Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigens, Surface (metabolism)</term>
<term>Apoptosis</term>
<term>Base Sequence</term>
<term>Cell Differentiation</term>
<term>Coronavirus</term>
<term>DNA, Viral (genetics)</term>
<term>Dendritic Cells (cytology)</term>
<term>Dendritic Cells (immunology)</term>
<term>Dendritic Cells (virology)</term>
<term>Dendritic cell</term>
<term>Humans</term>
<term>Immunity, Innate</term>
<term>In Vitro Techniques</term>
<term>Interferon-alpha (biosynthesis)</term>
<term>Microbiology</term>
<term>Phenotype</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (pathogenicity)</term>
<term>SARS Virus (physiology)</term>
<term>Severe acute respiratory syndrome</term>
<term>Virology</term>
<term>Virus Replication</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term>
<term>Antigènes de surface (métabolisme)</term>
<term>Apoptose</term>
<term>Cellules dendritiques (cytologie)</term>
<term>Cellules dendritiques (immunologie)</term>
<term>Cellules dendritiques (virologie)</term>
<term>Différenciation cellulaire</term>
<term>Humains</term>
<term>Immunité innée</term>
<term>Interféron alpha (biosynthèse)</term>
<term>Phénotype</term>
<term>Réplication virale</term>
<term>Séquence nucléotidique</term>
<term>Techniques in vitro</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (pathogénicité)</term>
<term>Virus du SRAS (physiologie)</term>
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<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Interféron alpha</term>
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<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Dendritic Cells</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term>
<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes de surface</term>
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<term>Base Sequence</term>
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<front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) of humans is caused by a novel coronavirus of zoonotic origin termed SARS-associated coronavirus (SARS-CoV). The virus induces severe injury of lung tissue, as well as lymphopenia and destruction of the architecture of lymphatic tissue by as-yet-unknown mechanisms. In this study, the interaction of SARS-CoV with dendritic cells (DCs), the key regulators of immune responses, was analysed. Monocyte-derived DCs were infected with SARS-CoV and analysed for viability, surface-marker expression and alpha interferon (IFN-α) induction. SARS-CoV infection was monitored by quantitative RT-PCR, immunofluorescence analysis and recovery experiments. SARS-CoV infected both immature and mature DCs, although replication efficiency was low. Immature DCs were activated by SARS-CoV infection and by UV-inactivated SARS-CoV. Infected DCs were still viable on day 6 post-infection, but major histocompatibility complex class I upregulation was missing, indicating that DC function was impaired. Additionally, SARS-CoV infection induced a delayed activation of IFN-a expression. Therefore, it is concluded that SARS-CoV has the ability to circumvent both the innate and the adaptive immune systems.</div>
</front>
</TEI>
<affiliations><list><country><li>Allemagne</li>
</country>
<region><li>Bade-Wurtemberg</li>
<li>Basse-Saxe</li>
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<tree><country name="Allemagne"><region name="Basse-Saxe"><name sortKey="Spiegel, Martin" sort="Spiegel, Martin" uniqKey="Spiegel M" first="Martin" last="Spiegel">Martin Spiegel</name>
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<name sortKey="Hufert, Frank T" sort="Hufert, Frank T" uniqKey="Hufert F" first="Frank T." last="Hufert">Frank T. Hufert</name>
<name sortKey="Schneider, Kerstin" sort="Schneider, Kerstin" uniqKey="Schneider K" first="Kerstin" last="Schneider">Kerstin Schneider</name>
<name sortKey="Weber, Friedemann" sort="Weber, Friedemann" uniqKey="Weber F" first="Friedemann" last="Weber">Friedemann Weber</name>
<name sortKey="Weidmann, Manfred" sort="Weidmann, Manfred" uniqKey="Weidmann M" first="Manfred" last="Weidmann">Manfred Weidmann</name>
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