Palmitoylations on murine coronavirus spike proteins are essential for virion assembly and infectivity.
Identifieur interne : 003D93 ( Main/Exploration ); précédent : 003D92; suivant : 003D94Palmitoylations on murine coronavirus spike proteins are essential for virion assembly and infectivity.
Auteurs : Edward B. Thorp [États-Unis] ; Joseph A. Boscarino ; Hillary L. Logan ; Jeffrey T. Goletz ; Thomas M. GallagherSource :
- Journal of virology [ 0022-538X ] ; 2006.
Descripteurs français
- KwdFr :
- Acides palmitiques (), Acylation, Acyltransferases (antagonistes et inhibiteurs), Acyltransferases (métabolisme), Animaux, Antienzymes (pharmacologie), Assemblage viral, Cellules HeLa, Cricetinae, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (physiologie), Humains, Lignée cellulaire, Maturation post-traductionnelle des protéines, Palmitates (pharmacologie), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (physiologie), Protéines de la matrice virale (physiologie), Recombinaison génétique, Souris, Virulence (), Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (pathogénicité), Virus de l'hépatite murine (physiologie).
- MESH :
- antagonistes et inhibiteurs : Acyltransferases.
- génétique : Virus de l'hépatite murine.
- métabolisme : Acyltransferases.
- pathogénicité : Virus de l'hépatite murine.
- pharmacologie : Antienzymes, Palmitates.
- physiologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines de la matrice virale, Virus de l'hépatite murine.
- Acides palmitiques, Acylation, Animaux, Assemblage viral, Cellules HeLa, Cricetinae, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Lignée cellulaire, Maturation post-traductionnelle des protéines, Protéines de l'enveloppe virale, Recombinaison génétique, Souris, Virulence.
English descriptors
- KwdEn :
- Acylation, Acyltransferases (antagonists & inhibitors), Acyltransferases (metabolism), Animals, Cell Line, Cricetinae, Enzyme Inhibitors (pharmacology), HeLa Cells, Humans, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (physiology), Mice, Murine hepatitis virus (genetics), Murine hepatitis virus (pathogenicity), Murine hepatitis virus (physiology), Palmitates (pharmacology), Palmitic Acids (chemistry), Protein Processing, Post-Translational, Recombination, Genetic, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (physiology), Viral Matrix Proteins (physiology), Virulence (drug effects), Virus Assembly.
- MESH :
- chemical , antagonists & inhibitors : Acyltransferases.
- chemical , chemistry : Membrane Glycoproteins, Palmitic Acids, Viral Envelope Proteins.
- chemical , metabolism : Acyltransferases.
- chemical , pharmacology : Enzyme Inhibitors, Palmitates.
- chemical , physiology : Membrane Glycoproteins, Viral Envelope Proteins, Viral Matrix Proteins.
- drug effects : Virulence.
- genetics : Murine hepatitis virus.
- pathogenicity : Murine hepatitis virus.
- physiology : Murine hepatitis virus.
- Acylation, Animals, Cell Line, Cricetinae, HeLa Cells, Humans, Mice, Protein Processing, Post-Translational, Recombination, Genetic, Spike Glycoprotein, Coronavirus, Virus Assembly.
Abstract
Coronavirus spike (S) proteins are palmitoylated at several cysteine residues clustered near their transmembrane-spanning domains. This is achieved by cellular palmitoyl acyltransferases (PATs), which can modify newly synthesized S proteins before they are assembled into virion envelopes at the intermediate compartment of the exocytic pathway. To address the importance of these fatty acylations to coronavirus infection, we exposed infected cells to 2-bromopalmitate (2-BP), a specific PAT inhibitor. 2-BP profoundly reduced the specific infectivities of murine coronaviruses at very low, nontoxic doses that were inert to alphavirus and rhabdovirus infections. 2-BP effected only two- to fivefold reductions in S palmitoylation, yet this correlated with reduced S complexing with virion membrane (M) proteins and consequent exclusion of S from virions. At defined 2-BP doses, underpalmitoylated S proteins instead trafficked to infected cell surfaces and elicited cell-cell membrane fusions, suggesting that the acyl chain adducts are more critical to virion assembly than to S-induced syncytial developments. These studies involving pharmacologic inhibition of S protein palmitoylation were complemented with molecular genetic analyses in which cysteine acylation substrates were mutated. Notably, some mutations (C1347F and C1348S) did not interfere with S incorporation into virions, indicating that only a subset of the cysteine-rich region provides the essential S-assembly functions. However, the C1347F/C1348S mutant viruses exhibited relatively low specific infectivities, similar to virions secreted from 2-BP-treated cultures. Our collective results indicate that the palmitate adducts on coronavirus S proteins are necessary in assembly and also in positioning the assembled envelope proteins for maximal infectivity.
DOI: 10.1128/JVI.80.3.1280-1289.2006
PubMed: 16415005
Affiliations:
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Le document en format XML
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acylation</term>
<term>Acyltransferases (antagonists & inhibitors)</term>
<term>Acyltransferases (metabolism)</term>
<term>Animals</term>
<term>Cell Line</term>
<term>Cricetinae</term>
<term>Enzyme Inhibitors (pharmacology)</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (physiology)</term>
<term>Mice</term>
<term>Murine hepatitis virus (genetics)</term>
<term>Murine hepatitis virus (pathogenicity)</term>
<term>Murine hepatitis virus (physiology)</term>
<term>Palmitates (pharmacology)</term>
<term>Palmitic Acids (chemistry)</term>
<term>Protein Processing, Post-Translational</term>
<term>Recombination, Genetic</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (physiology)</term>
<term>Viral Matrix Proteins (physiology)</term>
<term>Virulence (drug effects)</term>
<term>Virus Assembly</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acides palmitiques ()</term>
<term>Acylation</term>
<term>Acyltransferases (antagonistes et inhibiteurs)</term>
<term>Acyltransferases (métabolisme)</term>
<term>Animaux</term>
<term>Antienzymes (pharmacologie)</term>
<term>Assemblage viral</term>
<term>Cellules HeLa</term>
<term>Cricetinae</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires ()</term>
<term>Glycoprotéines membranaires (physiologie)</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Maturation post-traductionnelle des protéines</term>
<term>Palmitates (pharmacologie)</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Protéines de l'enveloppe virale (physiologie)</term>
<term>Protéines de la matrice virale (physiologie)</term>
<term>Recombinaison génétique</term>
<term>Souris</term>
<term>Virulence ()</term>
<term>Virus de l'hépatite murine (génétique)</term>
<term>Virus de l'hépatite murine (pathogénicité)</term>
<term>Virus de l'hépatite murine (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Acyltransferases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Palmitic Acids</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Acyltransferases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Enzyme Inhibitors</term>
<term>Palmitates</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Acyltransferases</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Virulence</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Murine hepatitis virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus de l'hépatite murine</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acyltransferases</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Murine hepatitis virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de l'hépatite murine</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antienzymes</term>
<term>Palmitates</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
<term>Virus de l'hépatite murine</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Murine hepatitis virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Acylation</term>
<term>Animals</term>
<term>Cell Line</term>
<term>Cricetinae</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Mice</term>
<term>Protein Processing, Post-Translational</term>
<term>Recombination, Genetic</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Virus Assembly</term>
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<keywords scheme="MESH" xml:lang="fr"><term>Acides palmitiques</term>
<term>Acylation</term>
<term>Animaux</term>
<term>Assemblage viral</term>
<term>Cellules HeLa</term>
<term>Cricetinae</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Maturation post-traductionnelle des protéines</term>
<term>Protéines de l'enveloppe virale</term>
<term>Recombinaison génétique</term>
<term>Souris</term>
<term>Virulence</term>
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<front><div type="abstract" xml:lang="en">Coronavirus spike (S) proteins are palmitoylated at several cysteine residues clustered near their transmembrane-spanning domains. This is achieved by cellular palmitoyl acyltransferases (PATs), which can modify newly synthesized S proteins before they are assembled into virion envelopes at the intermediate compartment of the exocytic pathway. To address the importance of these fatty acylations to coronavirus infection, we exposed infected cells to 2-bromopalmitate (2-BP), a specific PAT inhibitor. 2-BP profoundly reduced the specific infectivities of murine coronaviruses at very low, nontoxic doses that were inert to alphavirus and rhabdovirus infections. 2-BP effected only two- to fivefold reductions in S palmitoylation, yet this correlated with reduced S complexing with virion membrane (M) proteins and consequent exclusion of S from virions. At defined 2-BP doses, underpalmitoylated S proteins instead trafficked to infected cell surfaces and elicited cell-cell membrane fusions, suggesting that the acyl chain adducts are more critical to virion assembly than to S-induced syncytial developments. These studies involving pharmacologic inhibition of S protein palmitoylation were complemented with molecular genetic analyses in which cysteine acylation substrates were mutated. Notably, some mutations (C1347F and C1348S) did not interfere with S incorporation into virions, indicating that only a subset of the cysteine-rich region provides the essential S-assembly functions. However, the C1347F/C1348S mutant viruses exhibited relatively low specific infectivities, similar to virions secreted from 2-BP-treated cultures. Our collective results indicate that the palmitate adducts on coronavirus S proteins are necessary in assembly and also in positioning the assembled envelope proteins for maximal infectivity.</div>
</front>
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<region><li>Illinois</li>
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<tree><noCountry><name sortKey="Boscarino, Joseph A" sort="Boscarino, Joseph A" uniqKey="Boscarino J" first="Joseph A" last="Boscarino">Joseph A. Boscarino</name>
<name sortKey="Gallagher, Thomas M" sort="Gallagher, Thomas M" uniqKey="Gallagher T" first="Thomas M" last="Gallagher">Thomas M. Gallagher</name>
<name sortKey="Goletz, Jeffrey T" sort="Goletz, Jeffrey T" uniqKey="Goletz J" first="Jeffrey T" last="Goletz">Jeffrey T. Goletz</name>
<name sortKey="Logan, Hillary L" sort="Logan, Hillary L" uniqKey="Logan H" first="Hillary L" last="Logan">Hillary L. Logan</name>
</noCountry>
<country name="États-Unis"><region name="Illinois"><name sortKey="Thorp, Edward B" sort="Thorp, Edward B" uniqKey="Thorp E" first="Edward B" last="Thorp">Edward B. Thorp</name>
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