Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis
Identifieur interne : 004D75 ( Main/Exploration ); précédent : 004D74; suivant : 004D76Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis
Auteurs : Stephen A. Bustin [Royaume-Uni] ; Reinhold Mueller [États-Unis]Source :
- Clinical science : (1979) [ 0143-5221 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : ADN complémentaire.
- diagnostic : Maladies virales, Tumeurs.
- isolement et purification : Retroviridae, Virus à ARN.
- normes : RT-PCR.
- Pascal (Inist)
- Wicri :
English descriptors
- KwdEn :
- Colon, DNA, Complementary (biosynthesis), Detection, Diagnosis, Disease, Genetic Markers, Gut, Humans, Leukemia, Malignant tumor, Medicine, Neoplasms (diagnosis), Pathogenic, Polymerase chain reaction, Quality Control, RNA Viruses (isolation & purification), Real time, Retroviridae (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (standards), Reverse transcription polymerase chain reaction, Use, Virus, Virus Diseases (diagnosis).
- MESH :
- chemical , biosynthesis : DNA, Complementary.
- chemical : Genetic Markers.
- diagnosis : Neoplasms, Virus Diseases.
- isolation & purification : RNA Viruses, Retroviridae.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- standards : Reverse Transcriptase Polymerase Chain Reaction.
- Humans, Quality Control.
Abstract
qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection and quantification of RNA targets and is being utilized increasingly in novel clinical diagnostic assays. Quantitative results obtained by this technology are not only more informative than qualitative data, but simplify assay standardization and quality management. qRT-PCR assays are most established for the detection of viral load and therapy monitoring, and the development of SARS (severe acute respiratory syndrome)-associated coronavirus qRT-PCR assays provide a textbook example of the value of this technology for clinical diagnostics. The widespread use of qRT-PCR assays for diagnosis and the detection of disease-specific prognostic markers in leukaemia patients provide further examples of their usefulness. Their value for the detection of disease-associated mRNA expressed by circulating tumour cells in patients with solid malignancies is far less apparent, and the clinical significance of results obtained from such tests remains unclear. This is because of conceptual reservations as well as technical limitations that can interfere with the diagnostic specificity of qRT-PCR assays. Therefore, although it is evident that qRT-PCR assay has become a useful and important technology in the clinical diagnostic laboratory, it must be used appropriately and it is essential to be aware of its limitations if it is to fulfil its potential.
Affiliations:
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Le document en format XML
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Bustin</name><affiliation wicri:level="4"><inist:fA14 i1="01"><s1>Centre for Academic Surgery, Institute of Cell and Molecular Science, Barts and The London, Queen Mary's School of Medicine and Dentistry, University of London</s1><s2>London El IBB</s2><s3>GBR</s3><sZ>1 aut.</sZ></inist:fA14><country>Royaume-Uni</country><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName><orgName type="university">Université de Londres</orgName></affiliation></author><author><name sortKey="Mueller, Reinhold" sort="Mueller, Reinhold" uniqKey="Mueller R" first="Reinhold" last="Mueller">Reinhold Mueller</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Stratagene Inc., II0II N. Torrey Pines Road</s1><s2>La Jolla, CA 92037</s2><s3>USA</s3><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Californie</region></placeName></affiliation></author></analytic><series><title level="j" type="main">Clinical science : (1979)</title><title level="j" type="abbreviated">Clin. sci. : (1979)</title><idno type="ISSN">0143-5221</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Clinical science : (1979)</title><title level="j" type="abbreviated">Clin. sci. : (1979)</title><idno type="ISSN">0143-5221</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Colon</term><term>DNA, Complementary (biosynthesis)</term><term>Detection</term><term>Diagnosis</term><term>Disease</term><term>Genetic Markers</term><term>Gut</term><term>Humans</term><term>Leukemia</term><term>Malignant tumor</term><term>Medicine</term><term>Neoplasms (diagnosis)</term><term>Pathogenic</term><term>Polymerase chain reaction</term><term>Quality Control</term><term>RNA Viruses (isolation & purification)</term><term>Real time</term><term>Retroviridae (isolation & purification)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>Reverse Transcriptase Polymerase Chain Reaction (standards)</term><term>Reverse transcription polymerase chain reaction</term><term>Use</term><term>Virus</term><term>Virus Diseases (diagnosis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (biosynthèse)</term><term>Contrôle de qualité</term><term>Humains</term><term>Maladies virales (diagnostic)</term><term>Marqueurs génétiques</term><term>RT-PCR ()</term><term>RT-PCR (normes)</term><term>Retroviridae (isolement et purification)</term><term>Tumeurs (diagnostic)</term><term>Virus à ARN (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Genetic Markers</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ADN complémentaire</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Neoplasms</term><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Maladies virales</term><term>Tumeurs</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>RNA Viruses</term><term>Retroviridae</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Retroviridae</term><term>Virus à ARN</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>RT-PCR</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Quality Control</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Contrôle de qualité</term><term>Humains</term><term>Marqueurs génétiques</term><term>RT-PCR</term><term>Tumeur maligne</term><term>Temps réel</term><term>Réaction chaîne polymérase RT</term><term>Leucémie</term><term>Réaction chaîne polymérase</term><term>Utilisation</term><term>Diagnostic</term><term>Côlon</term><term>Intestin</term><term>Maladie</term><term>Pathogène</term><term>Virus</term><term>Détection</term><term>Médecine</term><term>Cancer</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Maladie</term><term>Médecine</term><term>Cancer</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection and quantification of RNA targets and is being utilized increasingly in novel clinical diagnostic assays. Quantitative results obtained by this technology are not only more informative than qualitative data, but simplify assay standardization and quality management. qRT-PCR assays are most established for the detection of viral load and therapy monitoring, and the development of SARS (severe acute respiratory syndrome)-associated coronavirus qRT-PCR assays provide a textbook example of the value of this technology for clinical diagnostics. The widespread use of qRT-PCR assays for diagnosis and the detection of disease-specific prognostic markers in leukaemia patients provide further examples of their usefulness. Their value for the detection of disease-associated mRNA expressed by circulating tumour cells in patients with solid malignancies is far less apparent, and the clinical significance of results obtained from such tests remains unclear. This is because of conceptual reservations as well as technical limitations that can interfere with the diagnostic specificity of qRT-PCR assays. Therefore, although it is evident that qRT-PCR assay has become a useful and important technology in the clinical diagnostic laboratory, it must be used appropriately and it is essential to be aware of its limitations if it is to fulfil its potential.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Angleterre</li><li>Californie</li><li>Grand Londres</li></region><settlement><li>Londres</li></settlement><orgName><li>Université de Londres</li></orgName></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Bustin, Stephen A" sort="Bustin, Stephen A" uniqKey="Bustin S" first="Stephen A." last="Bustin">Stephen A. Bustin</name></region></country><country name="États-Unis"><region name="Californie"><name sortKey="Mueller, Reinhold" sort="Mueller, Reinhold" uniqKey="Mueller R" first="Reinhold" last="Mueller">Reinhold Mueller</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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