Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis
Identifieur interne : 004D75 ( Main/Curation ); précédent : 004D74; suivant : 004D76Real-time reverse transcription PCR (qRT-PCR) and its potential use in clinical diagnosis
Auteurs : Stephen A. Bustin [Royaume-Uni] ; Reinhold Mueller [États-Unis]Source :
- Clinical science : (1979) [ 0143-5221 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- biosynthèse : ADN complémentaire.
- diagnostic : Maladies virales, Tumeurs.
- isolement et purification : Retroviridae, Virus à ARN.
- normes : RT-PCR.
- Pascal (Inist)
- Wicri :
English descriptors
- KwdEn :
- Colon, DNA, Complementary (biosynthesis), Detection, Diagnosis, Disease, Genetic Markers, Gut, Humans, Leukemia, Malignant tumor, Medicine, Neoplasms (diagnosis), Pathogenic, Polymerase chain reaction, Quality Control, RNA Viruses (isolation & purification), Real time, Retroviridae (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (standards), Reverse transcription polymerase chain reaction, Use, Virus, Virus Diseases (diagnosis).
- MESH :
- chemical , biosynthesis : DNA, Complementary.
- chemical : Genetic Markers.
- diagnosis : Neoplasms, Virus Diseases.
- isolation & purification : RNA Viruses, Retroviridae.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- standards : Reverse Transcriptase Polymerase Chain Reaction.
- Humans, Quality Control.
Abstract
qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection and quantification of RNA targets and is being utilized increasingly in novel clinical diagnostic assays. Quantitative results obtained by this technology are not only more informative than qualitative data, but simplify assay standardization and quality management. qRT-PCR assays are most established for the detection of viral load and therapy monitoring, and the development of SARS (severe acute respiratory syndrome)-associated coronavirus qRT-PCR assays provide a textbook example of the value of this technology for clinical diagnostics. The widespread use of qRT-PCR assays for diagnosis and the detection of disease-specific prognostic markers in leukaemia patients provide further examples of their usefulness. Their value for the detection of disease-associated mRNA expressed by circulating tumour cells in patients with solid malignancies is far less apparent, and the clinical significance of results obtained from such tests remains unclear. This is because of conceptual reservations as well as technical limitations that can interfere with the diagnostic specificity of qRT-PCR assays. Therefore, although it is evident that qRT-PCR assay has become a useful and important technology in the clinical diagnostic laboratory, it must be used appropriately and it is essential to be aware of its limitations if it is to fulfil its potential.
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Pascal:05-0441675Le document en format XML
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<front><div type="abstract" xml:lang="en">qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection and quantification of RNA targets and is being utilized increasingly in novel clinical diagnostic assays. Quantitative results obtained by this technology are not only more informative than qualitative data, but simplify assay standardization and quality management. qRT-PCR assays are most established for the detection of viral load and therapy monitoring, and the development of SARS (severe acute respiratory syndrome)-associated coronavirus qRT-PCR assays provide a textbook example of the value of this technology for clinical diagnostics. The widespread use of qRT-PCR assays for diagnosis and the detection of disease-specific prognostic markers in leukaemia patients provide further examples of their usefulness. Their value for the detection of disease-associated mRNA expressed by circulating tumour cells in patients with solid malignancies is far less apparent, and the clinical significance of results obtained from such tests remains unclear. This is because of conceptual reservations as well as technical limitations that can interfere with the diagnostic specificity of qRT-PCR assays. Therefore, although it is evident that qRT-PCR assay has become a useful and important technology in the clinical diagnostic laboratory, it must be used appropriately and it is essential to be aware of its limitations if it is to fulfil its potential.</div>
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<front><div type="abstract" xml:lang="en">qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection and quantification of RNA targets and is being utilized increasingly in novel clinical diagnostic assays. Quantitative results obtained by this technology are not only more informative than qualitative data, but simplify assay standardization and quality management. qRT-PCR assays are most established for the detection of viral load and therapy monitoring, and the development of SARS (severe acute respiratory syndrome)-associated coronavirus qRT-PCR assays provide a textbook example of the value of this technology for clinical diagnostics. The widespread use of qRT-PCR assays for diagnosis and the detection of disease-specific prognostic markers in leukaemia patients provide further examples of their usefulness. Their value for the detection of disease-associated mRNA expressed by circulating tumour cells in patients with solid malignancies is far less apparent, and the clinical significance of results obtained from such tests remains unclear. This is because of conceptual reservations as well as technical limitations that can interfere with the diagnostic specificity of qRT-PCR assays. Therefore, although it is evident that qRT-PCR assay has become a useful and important technology in the clinical diagnostic laboratory, it must be used appropriately and it is essential to be aware of its limitations if it is to fulfil its potential.</div>
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<term>RNA Viruses (isolation & purification)</term>
<term>Retroviridae (isolation & purification)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (standards)</term>
<term>Virus Diseases (diagnosis)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (biosynthèse)</term>
<term>Contrôle de qualité</term>
<term>Humains</term>
<term>Maladies virales (diagnostic)</term>
<term>Marqueurs génétiques</term>
<term>RT-PCR ()</term>
<term>RT-PCR (normes)</term>
<term>Retroviridae (isolement et purification)</term>
<term>Tumeurs (diagnostic)</term>
<term>Virus à ARN (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>DNA, Complementary</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Genetic Markers</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ADN complémentaire</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Neoplasms</term>
<term>Virus Diseases</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Maladies virales</term>
<term>Tumeurs</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>RNA Viruses</term>
<term>Retroviridae</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Retroviridae</term>
<term>Virus à ARN</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>RT-PCR</term>
</keywords>
<keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Humans</term>
<term>Quality Control</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Contrôle de qualité</term>
<term>Humains</term>
<term>Marqueurs génétiques</term>
<term>RT-PCR</term>
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<front><div type="abstract" xml:lang="en">qRT-PCR (real-time reverse transcription-PCR) has become the benchmark for the detection and quantification of RNA targets and is being utilized increasingly in novel clinical diagnostic assays. Quantitative results obtained by this technology are not only more informative than qualitative data, but simplify assay standardization and quality management. qRT-PCR assays are most established for the detection of viral load and therapy monitoring, and the development of SARS (severe acute respiratory syndrome)-associated coronavirus qRT-PCR assays provide a textbook example of the value of this technology for clinical diagnostics. The widespread use of qRT-PCR assays for diagnosis and the detection of disease-specific prognostic markers in leukaemia patients provide further examples of their usefulness. Their value for the detection of disease-associated mRNA expressed by circulating tumour cells in patients with solid malignancies is far less apparent, and the clinical significance of results obtained from such tests remains unclear. This is because of conceptual reservations as well as technical limitations that can interfere with the diagnostic specificity of qRT-PCR assays. Therefore, although it is evident that qRT-PCR assay has become a useful and important technology in the clinical diagnostic laboratory, it must be used appropriately and it is essential to be aware of its limitations if it is to fulfil its potential.</div>
</front>
</TEI>
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