Activation of NF-kappaB by the full-length nucleocapsid protein of the SARS coronavirus.
Identifieur interne : 004941 ( Main/Exploration ); précédent : 004940; suivant : 004942Activation of NF-kappaB by the full-length nucleocapsid protein of the SARS coronavirus.
Auteurs : Qing-Jiao Liao [République populaire de Chine] ; Lin-Bai Ye ; Khalid Amine Timani ; Ying-Chun Zeng ; Ying-Long She ; Li Ye ; Zheng-Hui WuSource :
- Acta biochimica et biophysica Sinica [ 1672-9145 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cellules HeLa, Cellules Vero, Facteur de transcription NF-kappa B (physiologie), Humains, Mutation, Protéines nucléocapside (), Protéines nucléocapside (génétique), Protéines nucléocapside (pharmacologie), Protéines nucléocapside (physiologie), Réplication virale, Signaux de localisation nucléaire, Virus du SRAS (), Virus du SRAS (physiologie).
- MESH :
- génétique : Protéines nucléocapside.
- pharmacologie : Protéines nucléocapside.
- physiologie : Facteur de transcription NF-kappa B, Protéines nucléocapside, Virus du SRAS.
- Animaux, Cellules HeLa, Cellules Vero, Humains, Mutation, Protéines nucléocapside, Réplication virale, Signaux de localisation nucléaire, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, HeLa Cells, Humans, Mutation, NF-kappa B (physiology), Nuclear Localization Signals, Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (pharmacology), Nucleocapsid Proteins (physiology), SARS Virus (chemistry), SARS Virus (physiology), Vero Cells, Virus Replication.
- MESH :
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , genetics : Nucleocapsid Proteins.
- chemical , pharmacology : Nucleocapsid Proteins.
- chemical , physiology : NF-kappa B, Nucleocapsid Proteins.
- chemistry : SARS Virus.
- physiology : SARS Virus.
- Animals, Chlorocebus aethiops, HeLa Cells, Humans, Mutation, Nuclear Localization Signals, Vero Cells, Virus Replication.
Abstract
The severe acute respiratory syndrome coronavirus (SARS-CoV) is the major causative agent for the worldwide outbreak of SARS in 2003. The mechanism by which SARS-CoV causes atypical pneumonia remains unclear. The nuclear factor kappa B (NF-kappaB) is a key transcription factor that activates numerous genes involved in cellular immune response and inflammation. Many studies have shown that NF-kappaB plays an important role in the pathogenesis of lung diseases. In this study, we investigated the possible regulatory interaction between the SARS-CoV nucleocapsid (N) protein and NF-kappaB by luciferase activity assay. Our results showed that the SARS-CoV N protein can significantly activate NF-kappaB only in Vero E6 cells, which are susceptible to SARS-CoV infection, but not in Vero or HeLa cells. This suggests that NF-kappaB activation is cell-specific. Furthermore, NF-kappaB activation in Vero E6 cells expressing the N protein is dose-dependent. Further experiments showed that there is more than one function domain in the N protein responsible for NF-kappaB activation. Our data indicated the possible role of the N protein in the pathogenesis of SARS.
DOI: 10.1111/j.1745-7270.2005.00082.x
PubMed: 16143815
Affiliations:
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Le document en format XML
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last="Timani">Khalid Amine Timani</name></author><author><name sortKey="Zeng, Ying Chun" sort="Zeng, Ying Chun" uniqKey="Zeng Y" first="Ying-Chun" last="Zeng">Ying-Chun Zeng</name></author><author><name sortKey="She, Ying Long" sort="She, Ying Long" uniqKey="She Y" first="Ying-Long" last="She">Ying-Long She</name></author><author><name sortKey="Ye, Li" sort="Ye, Li" uniqKey="Ye L" first="Li" last="Ye">Li Ye</name></author><author><name sortKey="Wu, Zheng Hui" sort="Wu, Zheng Hui" uniqKey="Wu Z" first="Zheng-Hui" last="Wu">Zheng-Hui Wu</name></author></analytic><series><title level="j">Acta biochimica et biophysica Sinica</title><idno type="ISSN">1672-9145</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>HeLa Cells</term><term>Humans</term><term>Mutation</term><term>NF-kappa B (physiology)</term><term>Nuclear Localization Signals</term><term>Nucleocapsid Proteins (chemistry)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (pharmacology)</term><term>Nucleocapsid Proteins (physiology)</term><term>SARS Virus (chemistry)</term><term>SARS Virus (physiology)</term><term>Vero Cells</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules HeLa</term><term>Cellules Vero</term><term>Facteur de transcription NF-kappa B (physiologie)</term><term>Humains</term><term>Mutation</term><term>Protéines nucléocapside ()</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (pharmacologie)</term><term>Protéines nucléocapside (physiologie)</term><term>Réplication virale</term><term>Signaux de localisation nucléaire</term><term>Virus du SRAS ()</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>NF-kappa B</term><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Facteur de transcription NF-kappa B</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>HeLa Cells</term><term>Humans</term><term>Mutation</term><term>Nuclear Localization Signals</term><term>Vero Cells</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules HeLa</term><term>Cellules Vero</term><term>Humains</term><term>Mutation</term><term>Protéines nucléocapside</term><term>Réplication virale</term><term>Signaux de localisation nucléaire</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome coronavirus (SARS-CoV) is the major causative agent for the worldwide outbreak of SARS in 2003. The mechanism by which SARS-CoV causes atypical pneumonia remains unclear. The nuclear factor kappa B (NF-kappaB) is a key transcription factor that activates numerous genes involved in cellular immune response and inflammation. Many studies have shown that NF-kappaB plays an important role in the pathogenesis of lung diseases. In this study, we investigated the possible regulatory interaction between the SARS-CoV nucleocapsid (N) protein and NF-kappaB by luciferase activity assay. Our results showed that the SARS-CoV N protein can significantly activate NF-kappaB only in Vero E6 cells, which are susceptible to SARS-CoV infection, but not in Vero or HeLa cells. This suggests that NF-kappaB activation is cell-specific. Furthermore, NF-kappaB activation in Vero E6 cells expressing the N protein is dose-dependent. Further experiments showed that there is more than one function domain in the N protein responsible for NF-kappaB activation. Our data indicated the possible role of the N protein in the pathogenesis of SARS.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><noCountry><name sortKey="She, Ying Long" sort="She, Ying Long" uniqKey="She Y" first="Ying-Long" last="She">Ying-Long She</name><name sortKey="Timani, Khalid Amine" sort="Timani, Khalid Amine" uniqKey="Timani K" first="Khalid Amine" last="Timani">Khalid Amine Timani</name><name sortKey="Wu, Zheng Hui" sort="Wu, Zheng Hui" uniqKey="Wu Z" first="Zheng-Hui" last="Wu">Zheng-Hui Wu</name><name sortKey="Ye, Li" sort="Ye, Li" uniqKey="Ye L" first="Li" last="Ye">Li Ye</name><name sortKey="Ye, Lin Bai" sort="Ye, Lin Bai" uniqKey="Ye L" first="Lin-Bai" last="Ye">Lin-Bai Ye</name><name sortKey="Zeng, Ying Chun" sort="Zeng, Ying Chun" uniqKey="Zeng Y" first="Ying-Chun" last="Zeng">Ying-Chun Zeng</name></noCountry><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Liao, Qing Jiao" sort="Liao, Qing Jiao" uniqKey="Liao Q" first="Qing-Jiao" last="Liao">Qing-Jiao Liao</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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