Activation of NF-kappaB by the full-length nucleocapsid protein of the SARS coronavirus.
Identifieur interne : 001148 ( Ncbi/Curation ); précédent : 001147; suivant : 001149Activation of NF-kappaB by the full-length nucleocapsid protein of the SARS coronavirus.
Auteurs : Qing-Jiao Liao [République populaire de Chine] ; Lin-Bai Ye ; Khalid Amine Timani ; Ying-Chun Zeng ; Ying-Long She ; Li Ye ; Zheng-Hui WuSource :
- Acta biochimica et biophysica Sinica [ 1672-9145 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cellules HeLa, Cellules Vero, Facteur de transcription NF-kappa B (physiologie), Humains, Mutation, Protéines nucléocapside (), Protéines nucléocapside (génétique), Protéines nucléocapside (pharmacologie), Protéines nucléocapside (physiologie), Réplication virale, Signaux de localisation nucléaire, Virus du SRAS (), Virus du SRAS (physiologie).
- MESH :
- génétique : Protéines nucléocapside.
- pharmacologie : Protéines nucléocapside.
- physiologie : Facteur de transcription NF-kappa B, Protéines nucléocapside, Virus du SRAS.
- Animaux, Cellules HeLa, Cellules Vero, Humains, Mutation, Protéines nucléocapside, Réplication virale, Signaux de localisation nucléaire, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, HeLa Cells, Humans, Mutation, NF-kappa B (physiology), Nuclear Localization Signals, Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (pharmacology), Nucleocapsid Proteins (physiology), SARS Virus (chemistry), SARS Virus (physiology), Vero Cells, Virus Replication.
- MESH :
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , genetics : Nucleocapsid Proteins.
- chemical , pharmacology : Nucleocapsid Proteins.
- chemical , physiology : NF-kappa B, Nucleocapsid Proteins.
- chemistry : SARS Virus.
- physiology : SARS Virus.
- Animals, Chlorocebus aethiops, HeLa Cells, Humans, Mutation, Nuclear Localization Signals, Vero Cells, Virus Replication.
Abstract
The severe acute respiratory syndrome coronavirus (SARS-CoV) is the major causative agent for the worldwide outbreak of SARS in 2003. The mechanism by which SARS-CoV causes atypical pneumonia remains unclear. The nuclear factor kappa B (NF-kappaB) is a key transcription factor that activates numerous genes involved in cellular immune response and inflammation. Many studies have shown that NF-kappaB plays an important role in the pathogenesis of lung diseases. In this study, we investigated the possible regulatory interaction between the SARS-CoV nucleocapsid (N) protein and NF-kappaB by luciferase activity assay. Our results showed that the SARS-CoV N protein can significantly activate NF-kappaB only in Vero E6 cells, which are susceptible to SARS-CoV infection, but not in Vero or HeLa cells. This suggests that NF-kappaB activation is cell-specific. Furthermore, NF-kappaB activation in Vero E6 cells expressing the N protein is dose-dependent. Further experiments showed that there is more than one function domain in the N protein responsible for NF-kappaB activation. Our data indicated the possible role of the N protein in the pathogenesis of SARS.
DOI: 10.1111/j.1745-7270.2005.00082.x
PubMed: 16143815
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pubmed:16143815Le document en format XML
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<term>NF-kappa B (physiology)</term>
<term>Nuclear Localization Signals</term>
<term>Nucleocapsid Proteins (chemistry)</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (pharmacology)</term>
<term>Nucleocapsid Proteins (physiology)</term>
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<term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (pharmacologie)</term>
<term>Protéines nucléocapside (physiologie)</term>
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<front><div type="abstract" xml:lang="en">The severe acute respiratory syndrome coronavirus (SARS-CoV) is the major causative agent for the worldwide outbreak of SARS in 2003. The mechanism by which SARS-CoV causes atypical pneumonia remains unclear. The nuclear factor kappa B (NF-kappaB) is a key transcription factor that activates numerous genes involved in cellular immune response and inflammation. Many studies have shown that NF-kappaB plays an important role in the pathogenesis of lung diseases. In this study, we investigated the possible regulatory interaction between the SARS-CoV nucleocapsid (N) protein and NF-kappaB by luciferase activity assay. Our results showed that the SARS-CoV N protein can significantly activate NF-kappaB only in Vero E6 cells, which are susceptible to SARS-CoV infection, but not in Vero or HeLa cells. This suggests that NF-kappaB activation is cell-specific. Furthermore, NF-kappaB activation in Vero E6 cells expressing the N protein is dose-dependent. Further experiments showed that there is more than one function domain in the N protein responsible for NF-kappaB activation. Our data indicated the possible role of the N protein in the pathogenesis of SARS.</div>
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