Identification and characterization of the putative fusion peptide of the severe acute respiratory syndrome-associated coronavirus spike protein
Identifieur interne : 004E18 ( Main/Exploration ); précédent : 004E17; suivant : 004E19Identification and characterization of the putative fusion peptide of the severe acute respiratory syndrome-associated coronavirus spike protein
Auteurs : Bruno Jr Sainz [États-Unis] ; Joshua M. Rausch [États-Unis] ; William R. Gallaher [États-Unis] ; Robert F. Garry [États-Unis] ; William C. Wimley [États-Unis]Source :
- Journal of virology [ 0022-538X ] ; 2005.
Descripteurs français
- KwdFr :
- Dichroïsme circulaire, Données de séquences moléculaires, Fragments peptidiques (), Fragments peptidiques (génétique), Fragments peptidiques (physiologie), Fusion membranaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (physiologie), Lipides membranaires (), Liposomes, Modèles moléculaires, Protéines de fusion virale (), Protéines de fusion virale (génétique), Protéines de fusion virale (physiologie), Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (physiologie), Sous-unités de protéines, Structure secondaire des protéines, Séquence d'acides aminés, Techniques in vitro, Virus du SRAS (génétique), Virus du SRAS (physiologie).
- MESH :
- Pascal (Inist)
- Coronavirus, Dichroïsme circulaire, Données de séquences moléculaires, Fragments peptidiques, Fusion membranaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Identification, Lipides membranaires, Liposomes, Modèles moléculaires, Peptide, Protéine, Microbiologie, Protéines de fusion virale, Protéines de l'enveloppe virale, Sous-unités de protéines, Structure secondaire des protéines, Séquence d'acides aminés, Techniques in vitro, Virologie, Syndrome respiratoire aigu sévère.
English descriptors
- KwdEn :
- Amino Acid Sequence, Circular Dichroism, Coronavirus, Identification, In Vitro Techniques, Liposomes, Membrane Fusion, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (physiology), Membrane Lipids (chemistry), Microbiology, Models, Molecular, Molecular Sequence Data, Peptide Fragments (chemistry), Peptide Fragments (genetics), Peptide Fragments (physiology), Peptides, Protein, Protein Structure, Secondary, Protein Subunits, SARS Virus (genetics), SARS Virus (physiology), Severe acute respiratory syndrome, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (physiology), Viral Fusion Proteins (chemistry), Viral Fusion Proteins (genetics), Viral Fusion Proteins (physiology), Virology.
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Membrane Lipids, Peptide Fragments, Viral Envelope Proteins, Viral Fusion Proteins.
- chemical , genetics : Membrane Glycoproteins, Peptide Fragments, Viral Envelope Proteins, Viral Fusion Proteins.
- chemical , physiology : Membrane Glycoproteins, Peptide Fragments, Viral Envelope Proteins, Viral Fusion Proteins.
- chemical : Liposomes, Protein Subunits, Spike Glycoprotein, Coronavirus.
- genetics : SARS Virus.
- physiology : SARS Virus.
- Amino Acid Sequence, Circular Dichroism, In Vitro Techniques, Membrane Fusion, Models, Molecular, Molecular Sequence Data, Protein Structure, Secondary.
Abstract
Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is a newly identified member of the family Coronaviridae and poses a serious public health threat. Recent studies indicated that the SARS-CoV viral spike glycoprotein is a class I viral fusion protein. A fusion peptide present at the N-terminal region of class I viral fusion proteins is believed to initiate viral and cell membrane interactions and subsequent fusion. Although the SARS-CoV fusion protein heptad repeats have been well characterized, the fusion peptide has yet to be identified. Based on the conserved features of known viral fusion peptides and using Wimley and White interfacial hydrophobicity plots, we have identified two putative fusion peptides (SAItSWW-I and SARSWW-II) at the N terminus of the SARS-CoV S2 subunit. Both peptides are hydrophobic and rich in alanine, glycine, and/or phenylalanine residues and contain a canonical fusion tripeptide along with a central proline residue. Only the SARSWW-I peptide strongly partitioned into the membranes of large unilamellar vesicles (LUV), adopting a β-sheet structure. Likewise, only SA========Rgr;SWW-I induced the fusion of LUV and caused membrane leakage of vesicle contents at peptide/lipid ratios of 1:50 and 1:100, respectively. The activity of this synthetic peptide appeared to be dependent on its amino acid (aa) sequence, as scrambling the peptide rendered it unable to partition into LUV, assume a defined secondary structure, or induce both fusion and leakage of LUV. Based on the activity of SA========Rgr;SWW-I, we propose that the hydrophobic stretch of 19 aa corresponding to residues 770 to 788 is a fusion peptide of the SARS-CoV S2 subunit.
Affiliations:
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Le document en format XML
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Rausch</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biochemistry, Tulane University Health Sciences Center</s1><s2>New Orleans, Louisiana 70112</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New Orleans, Louisiana 70112</wicri:noRegion></affiliation></author><author><name sortKey="Gallaher, William R" sort="Gallaher, William R" uniqKey="Gallaher W" first="William R." last="Gallaher">William R. Gallaher</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Microbiology, Immunology and Parasitology, Louisiana State University Health Sciences Center</s1><s2>New Orleans, Louisiana 70112</s2><s3>USA</s3><sZ>3 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New Orleans, Louisiana 70112</wicri:noRegion></affiliation></author><author><name sortKey="Garry, Robert F" sort="Garry, Robert F" uniqKey="Garry R" first="Robert F." last="Garry">Robert F. Garry</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Microbiology and Immunology, Program in Molecular Pathogenesis and Immunity, Tulane University Health Sciences Center</s1><s2>New Orleans, Louisiana 70112</s2><s3>USA</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New Orleans, Louisiana 70112</wicri:noRegion></affiliation></author><author><name sortKey="Wimley, William C" sort="Wimley, William C" uniqKey="Wimley W" first="William C." last="Wimley">William C. Wimley</name><affiliation wicri:level="1"><inist:fA14 i1="02"><s1>Department of Biochemistry, Tulane University Health Sciences Center</s1><s2>New Orleans, Louisiana 70112</s2><s3>USA</s3><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>New Orleans, Louisiana 70112</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of virology</title><title level="j" type="abbreviated">J. virol.</title><idno type="ISSN">0022-538X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Circular Dichroism</term><term>Coronavirus</term><term>Identification</term><term>In Vitro Techniques</term><term>Liposomes</term><term>Membrane Fusion</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (physiology)</term><term>Membrane Lipids (chemistry)</term><term>Microbiology</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Peptide Fragments (chemistry)</term><term>Peptide Fragments (genetics)</term><term>Peptide Fragments (physiology)</term><term>Peptides</term><term>Protein</term><term>Protein Structure, Secondary</term><term>Protein Subunits</term><term>SARS Virus (genetics)</term><term>SARS Virus (physiology)</term><term>Severe acute respiratory syndrome</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (physiology)</term><term>Viral Fusion Proteins (chemistry)</term><term>Viral Fusion Proteins (genetics)</term><term>Viral Fusion Proteins (physiology)</term><term>Virology</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Dichroïsme circulaire</term><term>Données de séquences moléculaires</term><term>Fragments peptidiques ()</term><term>Fragments peptidiques (génétique)</term><term>Fragments peptidiques (physiologie)</term><term>Fusion membranaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (physiologie)</term><term>Lipides membranaires ()</term><term>Liposomes</term><term>Modèles moléculaires</term><term>Protéines de fusion virale ()</term><term>Protéines de fusion virale (génétique)</term><term>Protéines de fusion virale (physiologie)</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (physiologie)</term><term>Sous-unités de protéines</term><term>Structure secondaire des protéines</term><term>Séquence d'acides aminés</term><term>Techniques in vitro</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term><term>Membrane Lipids</term><term>Peptide Fragments</term><term>Viral Envelope Proteins</term><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Peptide Fragments</term><term>Viral Envelope Proteins</term><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Membrane Glycoproteins</term><term>Peptide Fragments</term><term>Viral Envelope Proteins</term><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Liposomes</term><term>Protein Subunits</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Fragments peptidiques</term><term>Glycoprotéines membranaires</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Fragments peptidiques</term><term>Glycoprotéines membranaires</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Circular Dichroism</term><term>In Vitro Techniques</term><term>Membrane Fusion</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Protein Structure, Secondary</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Coronavirus</term><term>Dichroïsme circulaire</term><term>Données de séquences moléculaires</term><term>Fragments peptidiques</term><term>Fusion membranaire</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Identification</term><term>Lipides membranaires</term><term>Liposomes</term><term>Modèles moléculaires</term><term>Peptide</term><term>Protéine</term><term>Microbiologie</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Sous-unités de protéines</term><term>Structure secondaire des protéines</term><term>Séquence d'acides aminés</term><term>Techniques in vitro</term><term>Virologie</term><term>Syndrome respiratoire aigu sévère</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is a newly identified member of the family Coronaviridae and poses a serious public health threat. Recent studies indicated that the SARS-CoV viral spike glycoprotein is a class I viral fusion protein. A fusion peptide present at the N-terminal region of class I viral fusion proteins is believed to initiate viral and cell membrane interactions and subsequent fusion. Although the SARS-CoV fusion protein heptad repeats have been well characterized, the fusion peptide has yet to be identified. Based on the conserved features of known viral fusion peptides and using Wimley and White interfacial hydrophobicity plots, we have identified two putative fusion peptides (SAItS<sub>WW-I</sub> and SARS<sub>WW-II</sub>) at the N terminus of the SARS-CoV S2 subunit. Both peptides are hydrophobic and rich in alanine, glycine, and/or phenylalanine residues and contain a canonical fusion tripeptide along with a central proline residue. Only the SARS<sub>WW-I</sub> peptide strongly partitioned into the membranes of large unilamellar vesicles (LUV), adopting a β-sheet structure. Likewise, only SA========Rgr;S<sub>WW-I</sub> induced the fusion of LUV and caused membrane leakage of vesicle contents at peptide/lipid ratios of 1:50 and 1:100, respectively. The activity of this synthetic peptide appeared to be dependent on its amino acid (aa) sequence, as scrambling the peptide rendered it unable to partition into LUV, assume a defined secondary structure, or induce both fusion and leakage of LUV. Based on the activity of SA========Rgr;S<sub>WW-I</sub>, we propose that the hydrophobic stretch of 19 aa corresponding to residues 770 to 788 is a fusion peptide of the SARS-CoV S2 subunit.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Sainz, Bruno Jr" sort="Sainz, Bruno Jr" uniqKey="Sainz B" first="Bruno Jr" last="Sainz">Bruno Jr Sainz</name></noRegion><name sortKey="Gallaher, William R" sort="Gallaher, William R" uniqKey="Gallaher W" first="William R." last="Gallaher">William R. Gallaher</name><name sortKey="Garry, Robert F" sort="Garry, Robert F" uniqKey="Garry R" first="Robert F." last="Garry">Robert F. Garry</name><name sortKey="Rausch, Joshua M" sort="Rausch, Joshua M" uniqKey="Rausch J" first="Joshua M." last="Rausch">Joshua M. Rausch</name><name sortKey="Wimley, William C" sort="Wimley, William C" uniqKey="Wimley W" first="William C." last="Wimley">William C. Wimley</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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