Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs.
Identifieur interne : 004783 ( Main/Exploration ); précédent : 004782; suivant : 004784Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs.
Auteurs : Yi Shi [République populaire de Chine] ; De Hua Yang ; Jie Xiong ; Jie Jia ; Bing Huang ; You Xin JinSource :
- Cell research [ 1001-0602 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Clonage moléculaire, Interférence par ARN, Petit ARN interférent (pharmacologie), Protéines de l'enveloppe virale (antagonistes et inhibiteurs), Protéines de l'enveloppe virale (biosynthèse), Protéines de l'enveloppe virale (génétique), Protéines de la matrice virale (antagonistes et inhibiteurs), Protéines de la matrice virale (biosynthèse), Protéines de la matrice virale (génétique), Protéines nucléocapside (antagonistes et inhibiteurs), Protéines nucléocapside (biosynthèse), Protéines nucléocapside (génétique), Protéines virales structurales (antagonistes et inhibiteurs), Protéines virales structurales (biosynthèse), Protéines virales structurales (génétique), Protéines à fluorescence verte (métabolisme), Régulation de l'expression des gènes viraux, Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
- antagonistes et inhibiteurs : Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines nucléocapside, Protéines virales structurales.
- biosynthèse : Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines nucléocapside, Protéines virales structurales.
- génétique : Protéines de l'enveloppe virale, Protéines de la matrice virale, Protéines nucléocapside, Protéines virales structurales, Virus du SRAS.
- métabolisme : Protéines à fluorescence verte, Virus du SRAS.
- pharmacologie : Petit ARN interférent.
- Animaux, Cellules Vero, Clonage moléculaire, Interférence par ARN, Régulation de l'expression des gènes viraux, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Cloning, Molecular, Gene Expression Regulation, Viral, Green Fluorescent Proteins (metabolism), Nucleocapsid Proteins (antagonists & inhibitors), Nucleocapsid Proteins (biosynthesis), Nucleocapsid Proteins (genetics), RNA Interference, RNA, Small Interfering (pharmacology), SARS Virus (drug effects), SARS Virus (genetics), SARS Virus (metabolism), Vero Cells, Viral Envelope Proteins (antagonists & inhibitors), Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (genetics), Viral Matrix Proteins (antagonists & inhibitors), Viral Matrix Proteins (biosynthesis), Viral Matrix Proteins (genetics), Viral Structural Proteins (antagonists & inhibitors), Viral Structural Proteins (biosynthesis), Viral Structural Proteins (genetics).
- MESH :
- chemical , antagonists & inhibitors : Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Viral Structural Proteins.
- chemical , biosynthesis : Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Viral Structural Proteins.
- chemical , genetics : Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Viral Structural Proteins.
- chemical , metabolism : Green Fluorescent Proteins.
- chemical , pharmacology : RNA, Small Interfering.
- drug effects : SARS Virus.
- genetics : SARS Virus.
- metabolism : SARS Virus.
- Animals, Chlorocebus aethiops, Cloning, Molecular, Gene Expression Regulation, Viral, RNA Interference, Vero Cells.
Abstract
RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNA-mediated RNAi can be used in a wide variety of eucaryotes to induce the sequence-specific inhibition of gene expression. Synthetic 21-23 nucleotide (nt) small interfering RNA (siRNA) with 2 nt 3' overhangs was recently found to mediate efficient sequence-specific mRNA degradation in mammalian cells. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Among total 26 siRNA duplexes, we obtained 3 siRNA duplexes which could sequence-specifically reduce target genes expression over 80% at the concentration of 60 nM in Vero E6 cells. The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0 approximately 60 nM. Our results also showed that many inactive siRNA duplexes may be brought to life simply by unpairing the 5'end of the antisense strands. Results suggest that siRNA is capable of inhibiting SARS coronavirus genes expression and thus may be a new therapeutic strategy for treatment of SARS.
DOI: 10.1038/sj.cr.7290286
PubMed: 15780182
Affiliations:
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Le document en format XML
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sortKey="Xiong, Jie" sort="Xiong, Jie" uniqKey="Xiong J" first="Jie" last="Xiong">Jie Xiong</name></author><author><name sortKey="Jia, Jie" sort="Jia, Jie" uniqKey="Jia J" first="Jie" last="Jia">Jie Jia</name></author><author><name sortKey="Huang, Bing" sort="Huang, Bing" uniqKey="Huang B" first="Bing" last="Huang">Bing Huang</name></author><author><name sortKey="Jin, You Xin" sort="Jin, You Xin" uniqKey="Jin Y" first="You Xin" last="Jin">You Xin Jin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15780182</idno><idno type="pmid">15780182</idno><idno type="doi">10.1038/sj.cr.7290286</idno><idno type="wicri:Area/PubMed/Corpus">002835</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002835</idno><idno type="wicri:Area/PubMed/Curation">002835</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002835</idno><idno 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Jin</name></author></analytic><series><title level="j">Cell research</title><idno type="ISSN">1001-0602</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Cloning, Molecular</term><term>Gene Expression Regulation, Viral</term><term>Green Fluorescent Proteins (metabolism)</term><term>Nucleocapsid Proteins (antagonists & inhibitors)</term><term>Nucleocapsid Proteins (biosynthesis)</term><term>Nucleocapsid Proteins (genetics)</term><term>RNA Interference</term><term>RNA, Small Interfering (pharmacology)</term><term>SARS Virus (drug effects)</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>Vero Cells</term><term>Viral Envelope Proteins (antagonists & inhibitors)</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Matrix Proteins (antagonists & inhibitors)</term><term>Viral Matrix Proteins (biosynthesis)</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Structural Proteins (antagonists & inhibitors)</term><term>Viral Structural Proteins (biosynthesis)</term><term>Viral Structural Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Clonage moléculaire</term><term>Interférence par ARN</term><term>Petit ARN interférent (pharmacologie)</term><term>Protéines de l'enveloppe virale (antagonistes et inhibiteurs)</term><term>Protéines de l'enveloppe virale (biosynthèse)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de la matrice virale (antagonistes et inhibiteurs)</term><term>Protéines de la matrice virale (biosynthèse)</term><term>Protéines de la matrice virale (génétique)</term><term>Protéines nucléocapside (antagonistes et inhibiteurs)</term><term>Protéines nucléocapside (biosynthèse)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines virales structurales (antagonistes et inhibiteurs)</term><term>Protéines virales structurales (biosynthèse)</term><term>Protéines virales structurales (génétique)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Régulation de l'expression des gènes viraux</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines nucléocapside</term><term>Protéines virales structurales</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines nucléocapside</term><term>Protéines virales structurales</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de l'enveloppe virale</term><term>Protéines de la matrice virale</term><term>Protéines nucléocapside</term><term>Protéines virales structurales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines à fluorescence verte</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Petit ARN interférent</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Cloning, Molecular</term><term>Gene Expression Regulation, Viral</term><term>RNA Interference</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Clonage moléculaire</term><term>Interférence par ARN</term><term>Régulation de l'expression des gènes viraux</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNA-mediated RNAi can be used in a wide variety of eucaryotes to induce the sequence-specific inhibition of gene expression. Synthetic 21-23 nucleotide (nt) small interfering RNA (siRNA) with 2 nt 3' overhangs was recently found to mediate efficient sequence-specific mRNA degradation in mammalian cells. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Among total 26 siRNA duplexes, we obtained 3 siRNA duplexes which could sequence-specifically reduce target genes expression over 80% at the concentration of 60 nM in Vero E6 cells. The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0 approximately 60 nM. Our results also showed that many inactive siRNA duplexes may be brought to life simply by unpairing the 5'end of the antisense strands. Results suggest that siRNA is capable of inhibiting SARS coronavirus genes expression and thus may be a new therapeutic strategy for treatment of SARS.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Huang, Bing" sort="Huang, Bing" uniqKey="Huang B" first="Bing" last="Huang">Bing Huang</name><name sortKey="Jia, Jie" sort="Jia, Jie" uniqKey="Jia J" first="Jie" last="Jia">Jie Jia</name><name sortKey="Jin, You Xin" sort="Jin, You Xin" uniqKey="Jin Y" first="You Xin" last="Jin">You Xin Jin</name><name sortKey="Xiong, Jie" sort="Xiong, Jie" uniqKey="Xiong J" first="Jie" last="Xiong">Jie Xiong</name><name sortKey="Yang, De Hua" sort="Yang, De Hua" uniqKey="Yang D" first="De Hua" last="Yang">De Hua Yang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Shi, Yi" sort="Shi, Yi" uniqKey="Shi Y" first="Yi" last="Shi">Yi Shi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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