Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs.
Identifieur interne : 002835 ( PubMed/Corpus ); précédent : 002834; suivant : 002836Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs.
Auteurs : Yi Shi ; De Hua Yang ; Jie Xiong ; Jie Jia ; Bing Huang ; You Xin JinSource :
- Cell research [ 1001-0602 ] ; 2005.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Cloning, Molecular, Gene Expression Regulation, Viral, Green Fluorescent Proteins (metabolism), Nucleocapsid Proteins (antagonists & inhibitors), Nucleocapsid Proteins (biosynthesis), Nucleocapsid Proteins (genetics), RNA Interference, RNA, Small Interfering (pharmacology), SARS Virus (drug effects), SARS Virus (genetics), SARS Virus (metabolism), Vero Cells, Viral Envelope Proteins (antagonists & inhibitors), Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (genetics), Viral Matrix Proteins (antagonists & inhibitors), Viral Matrix Proteins (biosynthesis), Viral Matrix Proteins (genetics), Viral Structural Proteins (antagonists & inhibitors), Viral Structural Proteins (biosynthesis), Viral Structural Proteins (genetics).
- MESH :
- chemical , antagonists & inhibitors : Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Viral Structural Proteins.
- chemical , biosynthesis : Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Viral Structural Proteins.
- chemical , genetics : Nucleocapsid Proteins, Viral Envelope Proteins, Viral Matrix Proteins, Viral Structural Proteins.
- chemical , metabolism : Green Fluorescent Proteins.
- chemical , pharmacology : RNA, Small Interfering.
- drug effects : SARS Virus.
- genetics : SARS Virus.
- metabolism : SARS Virus.
- Animals, Chlorocebus aethiops, Cloning, Molecular, Gene Expression Regulation, Viral, RNA Interference, Vero Cells.
Abstract
RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNA-mediated RNAi can be used in a wide variety of eucaryotes to induce the sequence-specific inhibition of gene expression. Synthetic 21-23 nucleotide (nt) small interfering RNA (siRNA) with 2 nt 3' overhangs was recently found to mediate efficient sequence-specific mRNA degradation in mammalian cells. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Among total 26 siRNA duplexes, we obtained 3 siRNA duplexes which could sequence-specifically reduce target genes expression over 80% at the concentration of 60 nM in Vero E6 cells. The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0 approximately 60 nM. Our results also showed that many inactive siRNA duplexes may be brought to life simply by unpairing the 5'end of the antisense strands. Results suggest that siRNA is capable of inhibiting SARS coronavirus genes expression and thus may be a new therapeutic strategy for treatment of SARS.
DOI: 10.1038/sj.cr.7290286
PubMed: 15780182
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pubmed:15780182Le document en format XML
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Huang</name></author><author><name sortKey="Jin, You Xin" sort="Jin, You Xin" uniqKey="Jin Y" first="You Xin" last="Jin">You Xin Jin</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15780182</idno><idno type="pmid">15780182</idno><idno type="doi">10.1038/sj.cr.7290286</idno><idno type="wicri:Area/PubMed/Corpus">002835</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002835</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs.</title><author><name sortKey="Shi, Yi" sort="Shi, Yi" uniqKey="Shi Y" first="Yi" last="Shi">Yi Shi</name><affiliation><nlm:affiliation>State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yang, De Hua" sort="Yang, De Hua" uniqKey="Yang D" first="De Hua" last="Yang">De Hua Yang</name></author><author><name sortKey="Xiong, Jie" sort="Xiong, Jie" uniqKey="Xiong J" first="Jie" last="Xiong">Jie Xiong</name></author><author><name sortKey="Jia, Jie" sort="Jia, Jie" uniqKey="Jia J" first="Jie" last="Jia">Jie Jia</name></author><author><name sortKey="Huang, Bing" sort="Huang, Bing" uniqKey="Huang B" first="Bing" last="Huang">Bing Huang</name></author><author><name sortKey="Jin, You Xin" sort="Jin, You Xin" uniqKey="Jin Y" first="You Xin" last="Jin">You Xin Jin</name></author></analytic><series><title level="j">Cell research</title><idno type="ISSN">1001-0602</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Cloning, Molecular</term><term>Gene Expression Regulation, Viral</term><term>Green Fluorescent Proteins (metabolism)</term><term>Nucleocapsid Proteins (antagonists & inhibitors)</term><term>Nucleocapsid Proteins (biosynthesis)</term><term>Nucleocapsid Proteins (genetics)</term><term>RNA Interference</term><term>RNA, Small Interfering (pharmacology)</term><term>SARS Virus (drug effects)</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>Vero Cells</term><term>Viral Envelope Proteins (antagonists & inhibitors)</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Matrix Proteins (antagonists & inhibitors)</term><term>Viral Matrix Proteins (biosynthesis)</term><term>Viral Matrix Proteins (genetics)</term><term>Viral Structural Proteins (antagonists & inhibitors)</term><term>Viral Structural Proteins (biosynthesis)</term><term>Viral Structural Proteins (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Matrix Proteins</term><term>Viral Structural Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Green Fluorescent Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>RNA, Small Interfering</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Cloning, Molecular</term><term>Gene Expression Regulation, Viral</term><term>RNA Interference</term><term>Vero Cells</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNA-mediated RNAi can be used in a wide variety of eucaryotes to induce the sequence-specific inhibition of gene expression. Synthetic 21-23 nucleotide (nt) small interfering RNA (siRNA) with 2 nt 3' overhangs was recently found to mediate efficient sequence-specific mRNA degradation in mammalian cells. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Among total 26 siRNA duplexes, we obtained 3 siRNA duplexes which could sequence-specifically reduce target genes expression over 80% at the concentration of 60 nM in Vero E6 cells. The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0 approximately 60 nM. Our results also showed that many inactive siRNA duplexes may be brought to life simply by unpairing the 5'end of the antisense strands. Results suggest that siRNA is capable of inhibiting SARS coronavirus genes expression and thus may be a new therapeutic strategy for treatment of SARS.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15780182</PMID><DateCompleted><Year>2005</Year><Month>12</Month><Day>09</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1001-0602</ISSN><JournalIssue CitedMedium="Print"><Volume>15</Volume><Issue>3</Issue><PubDate><Year>2005</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Cell research</Title><ISOAbbreviation>Cell Res.</ISOAbbreviation></Journal><ArticleTitle>Inhibition of genes expression of SARS coronavirus by synthetic small interfering RNAs.</ArticleTitle><Pagination><MedlinePgn>193-200</MedlinePgn></Pagination><Abstract><AbstractText>RNA interference (RNAi) is triggered by the presence of a double-stranded RNA (dsRNA), and results in the silencing of homologous gene expression through the specific degradation of an mRNA containing the same sequence. dsRNA-mediated RNAi can be used in a wide variety of eucaryotes to induce the sequence-specific inhibition of gene expression. Synthetic 21-23 nucleotide (nt) small interfering RNA (siRNA) with 2 nt 3' overhangs was recently found to mediate efficient sequence-specific mRNA degradation in mammalian cells. Here, we studied the effects of synthetic siRNA duplexes targeted to SARS coronavirus structural proteins E, M, and N in a cell culture system. Among total 26 siRNA duplexes, we obtained 3 siRNA duplexes which could sequence-specifically reduce target genes expression over 80% at the concentration of 60 nM in Vero E6 cells. The downregulation effect was in correlation with the concentrations of the siRNA duplexes in a range of 0 approximately 60 nM. Our results also showed that many inactive siRNA duplexes may be brought to life simply by unpairing the 5'end of the antisense strands. Results suggest that siRNA is capable of inhibiting SARS coronavirus genes expression and thus may be a new therapeutic strategy for treatment of SARS.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Yi</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>De Hua</ForeName><Initials>DH</Initials></Author><Author ValidYN="Y"><LastName>Xiong</LastName><ForeName>Jie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Jia</LastName><ForeName>Jie</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Bing</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>You Xin</ForeName><Initials>YX</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Cell Res</MedlineTA><NlmUniqueID>9425763</NlmUniqueID><ISSNLinking>1001-0602</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C501689">E protein, SARS coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D034741">RNA, Small Interfering</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014759">Viral Envelope 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