Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression.
Identifieur interne : 005322 ( Main/Exploration ); précédent : 005321; suivant : 005323Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression.
Auteurs : Yuchun Nie [République populaire de Chine] ; Peigang Wang ; Xuanling Shi ; Guangwen Wang ; Jian Chen ; Aihua Zheng ; Wei Wang ; Zai Wang ; Xiuxia Qu ; Min Luo ; Lei Tan ; Xijun Song ; Xiaolei Yin ; Jianguo Chen ; Mingxiao Ding ; Hongkui DengSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- ADN recombiné (génétique), Animaux, Assemblage viral, Carboxypeptidases (génétique), Carboxypeptidases (physiologie), Cellules Vero, Chimère (génétique), Concentration en ions d'hydrogène, Expression des gènes, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Gènes viraux, Humains, Lignée cellulaire, Peptidyl-Dipeptidase A, Protéines de l'enveloppe virale (génétique), Récepteurs viraux (génétique), Récepteurs viraux (physiologie), Souris, Séquence nucléotidique, VIH (Virus de l'Immunodéficience Humaine) (génétique), Virulence (génétique), Virulence (physiologie), Virus du SRAS (génétique), Virus du SRAS (pathogénicité), Virus du SRAS (physiologie).
- MESH :
- génétique : ADN recombiné, Carboxypeptidases, Chimère, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Récepteurs viraux, VIH (Virus de l'Immunodéficience Humaine), Virulence, Virus du SRAS.
- pathogénicité : Virus du SRAS.
- physiologie : Carboxypeptidases, Récepteurs viraux, Virulence, Virus du SRAS.
- Animaux, Assemblage viral, Cellules Vero, Concentration en ions d'hydrogène, Expression des gènes, Glycoprotéine de spicule des coronavirus, Gènes viraux, Humains, Lignée cellulaire, Peptidyl-Dipeptidase A, Souris, Séquence nucléotidique.
English descriptors
- KwdEn :
- Animals, Base Sequence, Carboxypeptidases (genetics), Carboxypeptidases (physiology), Cell Line, Chimera (genetics), Chlorocebus aethiops, DNA, Recombinant (genetics), Gene Expression, Genes, Viral, HIV (genetics), Humans, Hydrogen-Ion Concentration, Membrane Glycoproteins (genetics), Mice, Peptidyl-Dipeptidase A, Receptors, Virus (genetics), Receptors, Virus (physiology), SARS Virus (genetics), SARS Virus (pathogenicity), SARS Virus (physiology), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (genetics), Virulence (genetics), Virulence (physiology), Virus Assembly.
- MESH :
- chemical , genetics : Carboxypeptidases, DNA, Recombinant, Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins.
- chemical , physiology : Carboxypeptidases, Receptors, Virus.
- genetics : Chimera, HIV, SARS Virus, Virulence.
- pathogenicity : SARS Virus.
- physiology : SARS Virus, Virulence.
- Animals, Base Sequence, Cell Line, Chlorocebus aethiops, Gene Expression, Genes, Viral, Humans, Hydrogen-Ion Concentration, Mice, Peptidyl-Dipeptidase A, Spike Glycoprotein, Coronavirus, Vero Cells, Virus Assembly.
Abstract
Studies of SARS coronavirus (SARS-CoV)-the causative agent of severe acute respiratory syndrome (SARS)-have been hampered by its high transmission rate and the pathogenicity of this virus. To permit analysis of the host range and entry mechanism of SARS-CoV, we incorporated the humanized SARS-CoV spike (S) glycoprotein into HIV particles to generate a highly infectious SARS-CoV pseudotyped virus. The infection on Vero E6-a permissive cell line to SARS-CoV-could be neutralized by sera from convalescent SARS patients, and the entry was a pH-dependent process. With these highly infectious SARS-CoV pseudotypes, several cell lines derived from various tissues were revealed as susceptible to SARS-CoV, which were highly corresponding to the expression pattern of virus's receptor angiotensin-converting enzyme 2 (ACE2). In addition, we also demonstrated angiotensin 1 converting enzyme (ACE)-the homologue of ACE2 could not function as a receptor for SARS-CoV.
DOI: 10.1016/j.bbrc.2004.07.060
PubMed: 15358126
Affiliations:
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cell tropism and its correlation with receptor expression.</title><author><name sortKey="Nie, Yuchun" sort="Nie, Yuchun" uniqKey="Nie Y" first="Yuchun" last="Nie">Yuchun Nie</name><affiliation wicri:level="4"><nlm:affiliation>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Cell Biology and Genetics, College of Life Sciences, Peking University, Beijing 100871</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName><orgName type="university">Université de Pékin</orgName><placeName><settlement type="city">Pékin</settlement><region type="capitale">Pékin</region></placeName></affiliation></author><author><name sortKey="Wang, Peigang" sort="Wang, Peigang" uniqKey="Wang P" first="Peigang" last="Wang">Peigang Wang</name></author><author><name sortKey="Shi, Xuanling" sort="Shi, Xuanling" uniqKey="Shi X" first="Xuanling" last="Shi">Xuanling Shi</name></author><author><name sortKey="Wang, Guangwen" sort="Wang, Guangwen" uniqKey="Wang G" first="Guangwen" last="Wang">Guangwen Wang</name></author><author><name sortKey="Chen, Jian" sort="Chen, Jian" uniqKey="Chen J" first="Jian" last="Chen">Jian Chen</name></author><author><name sortKey="Zheng, Aihua" sort="Zheng, Aihua" uniqKey="Zheng A" first="Aihua" last="Zheng">Aihua Zheng</name></author><author><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name></author><author><name sortKey="Wang, Zai" sort="Wang, Zai" uniqKey="Wang Z" first="Zai" last="Wang">Zai Wang</name></author><author><name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name></author><author><name sortKey="Luo, Min" sort="Luo, Min" uniqKey="Luo M" first="Min" last="Luo">Min Luo</name></author><author><name sortKey="Tan, Lei" sort="Tan, Lei" uniqKey="Tan L" first="Lei" 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xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Carboxypeptidases (genetics)</term><term>Carboxypeptidases (physiology)</term><term>Cell Line</term><term>Chimera (genetics)</term><term>Chlorocebus aethiops</term><term>DNA, Recombinant (genetics)</term><term>Gene Expression</term><term>Genes, Viral</term><term>HIV (genetics)</term><term>Humans</term><term>Hydrogen-Ion Concentration</term><term>Membrane Glycoproteins (genetics)</term><term>Mice</term><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus (genetics)</term><term>Receptors, Virus (physiology)</term><term>SARS Virus (genetics)</term><term>SARS Virus (pathogenicity)</term><term>SARS Virus (physiology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term><term>Viral Envelope Proteins (genetics)</term><term>Virulence (genetics)</term><term>Virulence (physiology)</term><term>Virus Assembly</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN recombiné (génétique)</term><term>Animaux</term><term>Assemblage viral</term><term>Carboxypeptidases (génétique)</term><term>Carboxypeptidases (physiologie)</term><term>Cellules Vero</term><term>Chimère (génétique)</term><term>Concentration en ions d'hydrogène</term><term>Expression des gènes</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (génétique)</term><term>Gènes viraux</term><term>Humains</term><term>Lignée cellulaire</term><term>Peptidyl-Dipeptidase A</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Récepteurs viraux (génétique)</term><term>Récepteurs viraux (physiologie)</term><term>Souris</term><term>Séquence nucléotidique</term><term>VIH (Virus de l'Immunodéficience Humaine) (génétique)</term><term>Virulence (génétique)</term><term>Virulence (physiologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (pathogénicité)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carboxypeptidases</term><term>DNA, Recombinant</term><term>Membrane Glycoproteins</term><term>Receptors, Virus</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Carboxypeptidases</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chimera</term><term>HIV</term><term>SARS Virus</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN recombiné</term><term>Carboxypeptidases</term><term>Chimère</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Récepteurs viraux</term><term>VIH (Virus de l'Immunodéficience Humaine)</term><term>Virulence</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Carboxypeptidases</term><term>Récepteurs viraux</term><term>Virulence</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Gene Expression</term><term>Genes, Viral</term><term>Humans</term><term>Hydrogen-Ion Concentration</term><term>Mice</term><term>Peptidyl-Dipeptidase A</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term><term>Virus Assembly</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Assemblage viral</term><term>Cellules Vero</term><term>Concentration en ions d'hydrogène</term><term>Expression des gènes</term><term>Glycoprotéine de spicule des coronavirus</term><term>Gènes viraux</term><term>Humains</term><term>Lignée cellulaire</term><term>Peptidyl-Dipeptidase A</term><term>Souris</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Studies of SARS coronavirus (SARS-CoV)-the causative agent of severe acute respiratory syndrome (SARS)-have been hampered by its high transmission rate and the pathogenicity of this virus. To permit analysis of the host range and entry mechanism of SARS-CoV, we incorporated the humanized SARS-CoV spike (S) glycoprotein into HIV particles to generate a highly infectious SARS-CoV pseudotyped virus. The infection on Vero E6-a permissive cell line to SARS-CoV-could be neutralized by sera from convalescent SARS patients, and the entry was a pH-dependent process. With these highly infectious SARS-CoV pseudotypes, several cell lines derived from various tissues were revealed as susceptible to SARS-CoV, which were highly corresponding to the expression pattern of virus's receptor angiotensin-converting enzyme 2 (ACE2). In addition, we also demonstrated angiotensin 1 converting enzyme (ACE)-the homologue of ACE2 could not function as a receptor for SARS-CoV.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Pékin</li></region><settlement><li>Pékin</li></settlement><orgName><li>Université de Pékin</li></orgName></list><tree><noCountry><name sortKey="Chen, Jian" sort="Chen, Jian" uniqKey="Chen J" first="Jian" last="Chen">Jian Chen</name><name sortKey="Chen, Jianguo" sort="Chen, Jianguo" uniqKey="Chen J" first="Jianguo" last="Chen">Jianguo Chen</name><name sortKey="Deng, Hongkui" sort="Deng, Hongkui" uniqKey="Deng H" first="Hongkui" last="Deng">Hongkui Deng</name><name sortKey="Ding, Mingxiao" sort="Ding, Mingxiao" uniqKey="Ding M" first="Mingxiao" last="Ding">Mingxiao Ding</name><name sortKey="Luo, Min" sort="Luo, Min" uniqKey="Luo M" first="Min" last="Luo">Min Luo</name><name sortKey="Qu, Xiuxia" sort="Qu, Xiuxia" uniqKey="Qu X" first="Xiuxia" last="Qu">Xiuxia Qu</name><name sortKey="Shi, Xuanling" sort="Shi, Xuanling" uniqKey="Shi X" first="Xuanling" last="Shi">Xuanling Shi</name><name sortKey="Song, Xijun" sort="Song, Xijun" uniqKey="Song X" first="Xijun" last="Song">Xijun Song</name><name sortKey="Tan, Lei" sort="Tan, Lei" uniqKey="Tan L" first="Lei" last="Tan">Lei Tan</name><name sortKey="Wang, Guangwen" sort="Wang, Guangwen" uniqKey="Wang G" first="Guangwen" last="Wang">Guangwen Wang</name><name sortKey="Wang, Peigang" sort="Wang, Peigang" uniqKey="Wang P" first="Peigang" last="Wang">Peigang Wang</name><name sortKey="Wang, Wei" sort="Wang, Wei" uniqKey="Wang W" first="Wei" last="Wang">Wei Wang</name><name sortKey="Wang, Zai" sort="Wang, Zai" uniqKey="Wang Z" first="Zai" last="Wang">Zai Wang</name><name sortKey="Yin, Xiaolei" sort="Yin, Xiaolei" uniqKey="Yin X" first="Xiaolei" last="Yin">Xiaolei Yin</name><name sortKey="Zheng, Aihua" sort="Zheng, Aihua" uniqKey="Zheng A" first="Aihua" last="Zheng">Aihua Zheng</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Nie, Yuchun" sort="Nie, Yuchun" uniqKey="Nie Y" first="Yuchun" last="Nie">Yuchun Nie</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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