A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice
Identifieur interne : 005A01 ( Main/Exploration ); précédent : 005A00; suivant : 005A02A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice
Auteurs : Naomi Takasuka ; Hideki Fujii ; Yoshimasa Takahashi ; Masataka Kasai ; Shigeru Morikawa ; Shigeyuki Itamura ; Koji Ishii ; Masahiro Sakaguchi ; Kazuo Ohnishi ; Masamichi Ohshima ; Shu-Ichi Hashimoto ; Takato Odagiri ; Masato Tashiro ; Hiroshi Yoshikura [Japon] ; Toshitada Takemori ; Yasuko Tsunetsugu-YokotaSource :
- International Immunology [ 0953-8178 ] ; 2004.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques (administration et posologie), Alun (administration et posologie), Animaux, Immunoglobuline G (sang), Injections sous-cutanées, Production d'anticorps, Protéines virales (immunologie), Souris, Spécificité des anticorps, Syndrome respiratoire aigu sévère (), Technique de Western, Vaccins antiviraux (administration et posologie), Vaccins inactivés (administration et posologie), Virion (immunologie), Virus du SRAS (immunologie).
- MESH :
- administration et posologie : Adjuvants immunologiques, Alun, Vaccins antiviraux, Vaccins inactivés.
- immunologie : Protéines virales, Virion, Virus du SRAS.
- sang : Immunoglobuline G.
- Animaux, Injections sous-cutanées, Production d'anticorps, Souris, Spécificité des anticorps, Syndrome respiratoire aigu sévère, Technique de Western.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (administration & dosage), Alum Compounds (administration & dosage), Animals, Antibody Formation, Antibody Specificity, Blotting, Western, Immunoglobulin G (blood), Injections, Subcutaneous, Mice, SARS Virus (immunology), Severe Acute Respiratory Syndrome (prevention & control), Vaccines, Inactivated (administration & dosage), Viral Proteins (immunology), Viral Vaccines (administration & dosage), Virion (immunology).
- MESH :
- chemical , administration & dosage : Adjuvants, Immunologic, Alum Compounds, Vaccines, Inactivated, Viral Vaccines.
- chemical , blood : Immunoglobulin G.
- immunology : SARS Virus, Viral Proteins, Virion.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Antibody Formation, Antibody Specificity, Blotting, Western, Injections, Subcutaneous, Mice.
Abstract
The recent emergence of severe acute respiratory syndrome (SARS) was caused by a novel coronavirus, SARS-CoV. It spread rapidly to many countries and developing a SARS vaccine is now urgently required. In order to study the immunogenicity of UV-inactivated purified SARS-CoV virion as a vaccine candidate, we subcutaneously immunized mice with UV-inactivated SARS-CoV with or without an adjuvant. We chose aluminum hydroxide gel (alum) as an adjuvant, because of its long safety history for human use. We observed that the UV-inactivated SARS-CoV virion elicited a high level of humoral immunity, resulting in the generation of long-term antibody secreting and memory B cells. With the addition of alum to the vaccine formula, serum IgG production was augmented and reached a level similar to that found in hyper-immunized mice, though it was still insufficient to elicit serum IgA antibodies. Notably, the SARS-CoV virion itself was able to induce long-term antibody production even without an adjuvant. Anti-SARS-CoV antibodies elicited in mice recognized both the spike and nucleocapsid proteins of the virus and were able to neutralize the virus. Furthermore, the UV-inactivated virion induced regional lymph node T-cell proliferation and significant levels of cytokine production (IL-2, IL-4, IL-5, IFN-γ and TNF-α) upon restimulation with inactivated SARS-CoV virion in vitro. Thus, a whole killed virion could serve as a candidate antigen for a SARS vaccine to elicit both humoral and cellular immunity.
Url:
- https://api.istex.fr/ark:/67375/HXZ-PDNM495K-Q/fulltext.pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108621
DOI: 10.1093/intimm/dxh143
Affiliations:
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Le document en format XML
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<term>Alum Compounds (administration & dosage)</term>
<term>Animals</term>
<term>Antibody Formation</term>
<term>Antibody Specificity</term>
<term>Blotting, Western</term>
<term>Immunoglobulin G (blood)</term>
<term>Injections, Subcutaneous</term>
<term>Mice</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (prevention & control)</term>
<term>Vaccines, Inactivated (administration & dosage)</term>
<term>Viral Proteins (immunology)</term>
<term>Viral Vaccines (administration & dosage)</term>
<term>Virion (immunology)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvants immunologiques (administration et posologie)</term>
<term>Alun (administration et posologie)</term>
<term>Animaux</term>
<term>Immunoglobuline G (sang)</term>
<term>Injections sous-cutanées</term>
<term>Production d'anticorps</term>
<term>Protéines virales (immunologie)</term>
<term>Souris</term>
<term>Spécificité des anticorps</term>
<term>Syndrome respiratoire aigu sévère ()</term>
<term>Technique de Western</term>
<term>Vaccins antiviraux (administration et posologie)</term>
<term>Vaccins inactivés (administration et posologie)</term>
<term>Virion (immunologie)</term>
<term>Virus du SRAS (immunologie)</term>
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<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Adjuvants, Immunologic</term>
<term>Alum Compounds</term>
<term>Vaccines, Inactivated</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Immunoglobulin G</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Adjuvants immunologiques</term>
<term>Alun</term>
<term>Vaccins antiviraux</term>
<term>Vaccins inactivés</term>
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<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines virales</term>
<term>Virion</term>
<term>Virus du SRAS</term>
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<term>Antibody Specificity</term>
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<front><div type="abstract" xml:lang="en">The recent emergence of severe acute respiratory syndrome (SARS) was caused by a novel coronavirus, SARS-CoV. It spread rapidly to many countries and developing a SARS vaccine is now urgently required. In order to study the immunogenicity of UV-inactivated purified SARS-CoV virion as a vaccine candidate, we subcutaneously immunized mice with UV-inactivated SARS-CoV with or without an adjuvant. We chose aluminum hydroxide gel (alum) as an adjuvant, because of its long safety history for human use. We observed that the UV-inactivated SARS-CoV virion elicited a high level of humoral immunity, resulting in the generation of long-term antibody secreting and memory B cells. With the addition of alum to the vaccine formula, serum IgG production was augmented and reached a level similar to that found in hyper-immunized mice, though it was still insufficient to elicit serum IgA antibodies. Notably, the SARS-CoV virion itself was able to induce long-term antibody production even without an adjuvant. Anti-SARS-CoV antibodies elicited in mice recognized both the spike and nucleocapsid proteins of the virus and were able to neutralize the virus. Furthermore, the UV-inactivated virion induced regional lymph node T-cell proliferation and significant levels of cytokine production (IL-2, IL-4, IL-5, IFN-γ and TNF-α) upon restimulation with inactivated SARS-CoV virion in vitro. Thus, a whole killed virion could serve as a candidate antigen for a SARS vaccine to elicit both humoral and cellular immunity.</div>
</front>
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<name sortKey="Itamura, Shigeyuki" sort="Itamura, Shigeyuki" uniqKey="Itamura S" first="Shigeyuki" last="Itamura">Shigeyuki Itamura</name>
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<name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
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<country name="Japon"><region name="Région de Kantō"><name sortKey="Yoshikura, Hiroshi" sort="Yoshikura, Hiroshi" uniqKey="Yoshikura H" first="Hiroshi" last="Yoshikura">Hiroshi Yoshikura</name>
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