A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice
Identifieur interne : 000A09 ( Ncbi/Merge ); précédent : 000A08; suivant : 000A10A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice
Auteurs : Naomi Takasuka ; Hideki Fujii ; Yoshimasa Takahashi ; Masataka Kasai ; Shigeru Morikawa ; Shigeyuki Itamura ; Koji Ishii ; Masahiro Sakaguchi ; Kazuo Ohnishi ; Masamichi Ohshima ; Shu-Ichi Hashimoto ; Takato Odagiri ; Masato Tashiro ; Hiroshi Yoshikura ; Toshitada Takemori ; Yasuko Tsunetsugu-YokotaSource :
- International Immunology [ 0953-8178 ] ; 2004.
Descripteurs français
- KwdFr :
- Adjuvants immunologiques (administration et posologie), Alun (administration et posologie), Animaux, Immunoglobuline G (sang), Injections sous-cutanées, Production d'anticorps, Protéines virales (immunologie), Souris, Spécificité des anticorps, Syndrome respiratoire aigu sévère (), Technique de Western, Vaccins antiviraux (administration et posologie), Vaccins inactivés (administration et posologie), Virion (immunologie), Virus du SRAS (immunologie).
- MESH :
- administration et posologie : Adjuvants immunologiques, Alun, Vaccins antiviraux, Vaccins inactivés.
- immunologie : Protéines virales, Virion, Virus du SRAS.
- sang : Immunoglobuline G.
- Animaux, Injections sous-cutanées, Production d'anticorps, Souris, Spécificité des anticorps, Syndrome respiratoire aigu sévère, Technique de Western.
English descriptors
- KwdEn :
- Adjuvants, Immunologic (administration & dosage), Alum Compounds (administration & dosage), Animals, Antibody Formation, Antibody Specificity, Blotting, Western, Immunoglobulin G (blood), Injections, Subcutaneous, Mice, SARS Virus (immunology), Severe Acute Respiratory Syndrome (prevention & control), Vaccines, Inactivated (administration & dosage), Viral Proteins (immunology), Viral Vaccines (administration & dosage), Virion (immunology).
- MESH :
- chemical , administration & dosage : Adjuvants, Immunologic, Alum Compounds, Vaccines, Inactivated, Viral Vaccines.
- chemical , blood : Immunoglobulin G.
- immunology : SARS Virus, Viral Proteins, Virion.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Antibody Formation, Antibody Specificity, Blotting, Western, Injections, Subcutaneous, Mice.
Abstract
The recent emergence of severe acute respiratory syndrome (SARS) was caused by a novel coronavirus, SARS-CoV. It spread rapidly to many countries and developing a SARS vaccine is now urgently required. In order to study the immunogenicity of UV-inactivated purified SARS-CoV virion as a vaccine candidate, we subcutaneously immunized mice with UV-inactivated SARS-CoV with or without an adjuvant. We chose aluminum hydroxide gel (alum) as an adjuvant, because of its long safety history for human use. We observed that the UV-inactivated SARS-CoV virion elicited a high level of humoral immunity, resulting in the generation of long-term antibody secreting and memory B cells. With the addition of alum to the vaccine formula, serum IgG production was augmented and reached a level similar to that found in hyper-immunized mice, though it was still insufficient to elicit serum IgA antibodies. Notably, the SARS-CoV virion itself was able to induce long-term antibody production even without an adjuvant. Anti-SARS-CoV antibodies elicited in mice recognized both the spike and nucleocapsid proteins of the virus and were able to neutralize the virus. Furthermore, the UV-inactivated virion induced regional lymph node T-cell proliferation and significant levels of cytokine production (IL-2, IL-4, IL-5, IFN-γ and TNF-α) upon restimulation with inactivated SARS-CoV virion
Url:
DOI: 10.1093/intimm/dxh143
PubMed: 15314040
PubMed Central: 7108621
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000B67
- to stream Pmc, to step Curation: 000B67
- to stream Pmc, to step Checkpoint: 001740
- to stream PubMed, to step Corpus: 002C13
- to stream PubMed, to step Curation: 002C13
- to stream PubMed, to step Checkpoint: 002E52
Links to Exploration step
PMC:7108621Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice</title>
<author><name sortKey="Takasuka, Naomi" sort="Takasuka, Naomi" uniqKey="Takasuka N" first="Naomi" last="Takasuka">Naomi Takasuka</name>
</author>
<author><name sortKey="Fujii, Hideki" sort="Fujii, Hideki" uniqKey="Fujii H" first="Hideki" last="Fujii">Hideki Fujii</name>
</author>
<author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name>
</author>
<author><name sortKey="Kasai, Masataka" sort="Kasai, Masataka" uniqKey="Kasai M" first="Masataka" last="Kasai">Masataka Kasai</name>
</author>
<author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name>
</author>
<author><name sortKey="Itamura, Shigeyuki" sort="Itamura, Shigeyuki" uniqKey="Itamura S" first="Shigeyuki" last="Itamura">Shigeyuki Itamura</name>
</author>
<author><name sortKey="Ishii, Koji" sort="Ishii, Koji" uniqKey="Ishii K" first="Koji" last="Ishii">Koji Ishii</name>
</author>
<author><name sortKey="Sakaguchi, Masahiro" sort="Sakaguchi, Masahiro" uniqKey="Sakaguchi M" first="Masahiro" last="Sakaguchi">Masahiro Sakaguchi</name>
</author>
<author><name sortKey="Ohnishi, Kazuo" sort="Ohnishi, Kazuo" uniqKey="Ohnishi K" first="Kazuo" last="Ohnishi">Kazuo Ohnishi</name>
</author>
<author><name sortKey="Ohshima, Masamichi" sort="Ohshima, Masamichi" uniqKey="Ohshima M" first="Masamichi" last="Ohshima">Masamichi Ohshima</name>
</author>
<author><name sortKey="Hashimoto, Shu Ichi" sort="Hashimoto, Shu Ichi" uniqKey="Hashimoto S" first="Shu-Ichi" last="Hashimoto">Shu-Ichi Hashimoto</name>
</author>
<author><name sortKey="Odagiri, Takato" sort="Odagiri, Takato" uniqKey="Odagiri T" first="Takato" last="Odagiri">Takato Odagiri</name>
</author>
<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
</author>
<author><name sortKey="Yoshikura, Hiroshi" sort="Yoshikura, Hiroshi" uniqKey="Yoshikura H" first="Hiroshi" last="Yoshikura">Hiroshi Yoshikura</name>
</author>
<author><name sortKey="Takemori, Toshitada" sort="Takemori, Toshitada" uniqKey="Takemori T" first="Toshitada" last="Takemori">Toshitada Takemori</name>
</author>
<author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">15314040</idno>
<idno type="pmc">7108621</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108621</idno>
<idno type="RBID">PMC:7108621</idno>
<idno type="doi">10.1093/intimm/dxh143</idno>
<date when="2004">2004</date>
<idno type="wicri:Area/Pmc/Corpus">000B67</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000B67</idno>
<idno type="wicri:Area/Pmc/Curation">000B67</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000B67</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001740</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001740</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:15314040</idno>
<idno type="wicri:Area/PubMed/Corpus">002C13</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002C13</idno>
<idno type="wicri:Area/PubMed/Curation">002C13</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002C13</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002E52</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002E52</idno>
<idno type="wicri:Area/Ncbi/Merge">000A09</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice</title>
<author><name sortKey="Takasuka, Naomi" sort="Takasuka, Naomi" uniqKey="Takasuka N" first="Naomi" last="Takasuka">Naomi Takasuka</name>
</author>
<author><name sortKey="Fujii, Hideki" sort="Fujii, Hideki" uniqKey="Fujii H" first="Hideki" last="Fujii">Hideki Fujii</name>
</author>
<author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name>
</author>
<author><name sortKey="Kasai, Masataka" sort="Kasai, Masataka" uniqKey="Kasai M" first="Masataka" last="Kasai">Masataka Kasai</name>
</author>
<author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name>
</author>
<author><name sortKey="Itamura, Shigeyuki" sort="Itamura, Shigeyuki" uniqKey="Itamura S" first="Shigeyuki" last="Itamura">Shigeyuki Itamura</name>
</author>
<author><name sortKey="Ishii, Koji" sort="Ishii, Koji" uniqKey="Ishii K" first="Koji" last="Ishii">Koji Ishii</name>
</author>
<author><name sortKey="Sakaguchi, Masahiro" sort="Sakaguchi, Masahiro" uniqKey="Sakaguchi M" first="Masahiro" last="Sakaguchi">Masahiro Sakaguchi</name>
</author>
<author><name sortKey="Ohnishi, Kazuo" sort="Ohnishi, Kazuo" uniqKey="Ohnishi K" first="Kazuo" last="Ohnishi">Kazuo Ohnishi</name>
</author>
<author><name sortKey="Ohshima, Masamichi" sort="Ohshima, Masamichi" uniqKey="Ohshima M" first="Masamichi" last="Ohshima">Masamichi Ohshima</name>
</author>
<author><name sortKey="Hashimoto, Shu Ichi" sort="Hashimoto, Shu Ichi" uniqKey="Hashimoto S" first="Shu-Ichi" last="Hashimoto">Shu-Ichi Hashimoto</name>
</author>
<author><name sortKey="Odagiri, Takato" sort="Odagiri, Takato" uniqKey="Odagiri T" first="Takato" last="Odagiri">Takato Odagiri</name>
</author>
<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
</author>
<author><name sortKey="Yoshikura, Hiroshi" sort="Yoshikura, Hiroshi" uniqKey="Yoshikura H" first="Hiroshi" last="Yoshikura">Hiroshi Yoshikura</name>
</author>
<author><name sortKey="Takemori, Toshitada" sort="Takemori, Toshitada" uniqKey="Takemori T" first="Toshitada" last="Takemori">Toshitada Takemori</name>
</author>
<author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
</author>
</analytic>
<series><title level="j">International Immunology</title>
<idno type="ISSN">0953-8178</idno>
<idno type="eISSN">1460-2377</idno>
<imprint><date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic (administration & dosage)</term>
<term>Alum Compounds (administration & dosage)</term>
<term>Animals</term>
<term>Antibody Formation</term>
<term>Antibody Specificity</term>
<term>Blotting, Western</term>
<term>Immunoglobulin G (blood)</term>
<term>Injections, Subcutaneous</term>
<term>Mice</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (prevention & control)</term>
<term>Vaccines, Inactivated (administration & dosage)</term>
<term>Viral Proteins (immunology)</term>
<term>Viral Vaccines (administration & dosage)</term>
<term>Virion (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvants immunologiques (administration et posologie)</term>
<term>Alun (administration et posologie)</term>
<term>Animaux</term>
<term>Immunoglobuline G (sang)</term>
<term>Injections sous-cutanées</term>
<term>Production d'anticorps</term>
<term>Protéines virales (immunologie)</term>
<term>Souris</term>
<term>Spécificité des anticorps</term>
<term>Syndrome respiratoire aigu sévère ()</term>
<term>Technique de Western</term>
<term>Vaccins antiviraux (administration et posologie)</term>
<term>Vaccins inactivés (administration et posologie)</term>
<term>Virion (immunologie)</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Adjuvants, Immunologic</term>
<term>Alum Compounds</term>
<term>Vaccines, Inactivated</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Immunoglobulin G</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Adjuvants immunologiques</term>
<term>Alun</term>
<term>Vaccins antiviraux</term>
<term>Vaccins inactivés</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines virales</term>
<term>Virion</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
<term>Viral Proteins</term>
<term>Virion</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Immunoglobuline G</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Antibody Formation</term>
<term>Antibody Specificity</term>
<term>Blotting, Western</term>
<term>Injections, Subcutaneous</term>
<term>Mice</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Injections sous-cutanées</term>
<term>Production d'anticorps</term>
<term>Souris</term>
<term>Spécificité des anticorps</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Technique de Western</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p>The recent emergence of severe acute respiratory syndrome (SARS) was caused by a novel coronavirus, SARS-CoV. It spread rapidly to many countries and developing a SARS vaccine is now urgently required. In order to study the immunogenicity of UV-inactivated purified SARS-CoV virion as a vaccine candidate, we subcutaneously immunized mice with UV-inactivated SARS-CoV with or without an adjuvant. We chose aluminum hydroxide gel (alum) as an adjuvant, because of its long safety history for human use. We observed that the UV-inactivated SARS-CoV virion elicited a high level of humoral immunity, resulting in the generation of long-term antibody secreting and memory B cells. With the addition of alum to the vaccine formula, serum IgG production was augmented and reached a level similar to that found in hyper-immunized mice, though it was still insufficient to elicit serum IgA antibodies. Notably, the SARS-CoV virion itself was able to induce long-term antibody production even without an adjuvant. Anti-SARS-CoV antibodies elicited in mice recognized both the spike and nucleocapsid proteins of the virus and were able to neutralize the virus. Furthermore, the UV-inactivated virion induced regional lymph node T-cell proliferation and significant levels of cytokine production (IL-2, IL-4, IL-5, IFN-γ and TNF-α) upon restimulation with inactivated SARS-CoV virion <italic>in vitro</italic>
. Thus, a whole killed virion could serve as a candidate antigen for a SARS vaccine to elicit both humoral and cellular immunity.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
<double pmid="15314040"><pmc><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice</title>
<author><name sortKey="Takasuka, Naomi" sort="Takasuka, Naomi" uniqKey="Takasuka N" first="Naomi" last="Takasuka">Naomi Takasuka</name>
</author>
<author><name sortKey="Fujii, Hideki" sort="Fujii, Hideki" uniqKey="Fujii H" first="Hideki" last="Fujii">Hideki Fujii</name>
</author>
<author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name>
</author>
<author><name sortKey="Kasai, Masataka" sort="Kasai, Masataka" uniqKey="Kasai M" first="Masataka" last="Kasai">Masataka Kasai</name>
</author>
<author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name>
</author>
<author><name sortKey="Itamura, Shigeyuki" sort="Itamura, Shigeyuki" uniqKey="Itamura S" first="Shigeyuki" last="Itamura">Shigeyuki Itamura</name>
</author>
<author><name sortKey="Ishii, Koji" sort="Ishii, Koji" uniqKey="Ishii K" first="Koji" last="Ishii">Koji Ishii</name>
</author>
<author><name sortKey="Sakaguchi, Masahiro" sort="Sakaguchi, Masahiro" uniqKey="Sakaguchi M" first="Masahiro" last="Sakaguchi">Masahiro Sakaguchi</name>
</author>
<author><name sortKey="Ohnishi, Kazuo" sort="Ohnishi, Kazuo" uniqKey="Ohnishi K" first="Kazuo" last="Ohnishi">Kazuo Ohnishi</name>
</author>
<author><name sortKey="Ohshima, Masamichi" sort="Ohshima, Masamichi" uniqKey="Ohshima M" first="Masamichi" last="Ohshima">Masamichi Ohshima</name>
</author>
<author><name sortKey="Hashimoto, Shu Ichi" sort="Hashimoto, Shu Ichi" uniqKey="Hashimoto S" first="Shu-Ichi" last="Hashimoto">Shu-Ichi Hashimoto</name>
</author>
<author><name sortKey="Odagiri, Takato" sort="Odagiri, Takato" uniqKey="Odagiri T" first="Takato" last="Odagiri">Takato Odagiri</name>
</author>
<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
</author>
<author><name sortKey="Yoshikura, Hiroshi" sort="Yoshikura, Hiroshi" uniqKey="Yoshikura H" first="Hiroshi" last="Yoshikura">Hiroshi Yoshikura</name>
</author>
<author><name sortKey="Takemori, Toshitada" sort="Takemori, Toshitada" uniqKey="Takemori T" first="Toshitada" last="Takemori">Toshitada Takemori</name>
</author>
<author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">15314040</idno>
<idno type="pmc">7108621</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108621</idno>
<idno type="RBID">PMC:7108621</idno>
<idno type="doi">10.1093/intimm/dxh143</idno>
<date when="2004">2004</date>
<idno type="wicri:Area/Pmc/Corpus">000B67</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000B67</idno>
<idno type="wicri:Area/Pmc/Curation">000B67</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000B67</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001740</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001740</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice</title>
<author><name sortKey="Takasuka, Naomi" sort="Takasuka, Naomi" uniqKey="Takasuka N" first="Naomi" last="Takasuka">Naomi Takasuka</name>
</author>
<author><name sortKey="Fujii, Hideki" sort="Fujii, Hideki" uniqKey="Fujii H" first="Hideki" last="Fujii">Hideki Fujii</name>
</author>
<author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name>
</author>
<author><name sortKey="Kasai, Masataka" sort="Kasai, Masataka" uniqKey="Kasai M" first="Masataka" last="Kasai">Masataka Kasai</name>
</author>
<author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name>
</author>
<author><name sortKey="Itamura, Shigeyuki" sort="Itamura, Shigeyuki" uniqKey="Itamura S" first="Shigeyuki" last="Itamura">Shigeyuki Itamura</name>
</author>
<author><name sortKey="Ishii, Koji" sort="Ishii, Koji" uniqKey="Ishii K" first="Koji" last="Ishii">Koji Ishii</name>
</author>
<author><name sortKey="Sakaguchi, Masahiro" sort="Sakaguchi, Masahiro" uniqKey="Sakaguchi M" first="Masahiro" last="Sakaguchi">Masahiro Sakaguchi</name>
</author>
<author><name sortKey="Ohnishi, Kazuo" sort="Ohnishi, Kazuo" uniqKey="Ohnishi K" first="Kazuo" last="Ohnishi">Kazuo Ohnishi</name>
</author>
<author><name sortKey="Ohshima, Masamichi" sort="Ohshima, Masamichi" uniqKey="Ohshima M" first="Masamichi" last="Ohshima">Masamichi Ohshima</name>
</author>
<author><name sortKey="Hashimoto, Shu Ichi" sort="Hashimoto, Shu Ichi" uniqKey="Hashimoto S" first="Shu-Ichi" last="Hashimoto">Shu-Ichi Hashimoto</name>
</author>
<author><name sortKey="Odagiri, Takato" sort="Odagiri, Takato" uniqKey="Odagiri T" first="Takato" last="Odagiri">Takato Odagiri</name>
</author>
<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
</author>
<author><name sortKey="Yoshikura, Hiroshi" sort="Yoshikura, Hiroshi" uniqKey="Yoshikura H" first="Hiroshi" last="Yoshikura">Hiroshi Yoshikura</name>
</author>
<author><name sortKey="Takemori, Toshitada" sort="Takemori, Toshitada" uniqKey="Takemori T" first="Toshitada" last="Takemori">Toshitada Takemori</name>
</author>
<author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
</author>
</analytic>
<series><title level="j">International Immunology</title>
<idno type="ISSN">0953-8178</idno>
<idno type="eISSN">1460-2377</idno>
<imprint><date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p>The recent emergence of severe acute respiratory syndrome (SARS) was caused by a novel coronavirus, SARS-CoV. It spread rapidly to many countries and developing a SARS vaccine is now urgently required. In order to study the immunogenicity of UV-inactivated purified SARS-CoV virion as a vaccine candidate, we subcutaneously immunized mice with UV-inactivated SARS-CoV with or without an adjuvant. We chose aluminum hydroxide gel (alum) as an adjuvant, because of its long safety history for human use. We observed that the UV-inactivated SARS-CoV virion elicited a high level of humoral immunity, resulting in the generation of long-term antibody secreting and memory B cells. With the addition of alum to the vaccine formula, serum IgG production was augmented and reached a level similar to that found in hyper-immunized mice, though it was still insufficient to elicit serum IgA antibodies. Notably, the SARS-CoV virion itself was able to induce long-term antibody production even without an adjuvant. Anti-SARS-CoV antibodies elicited in mice recognized both the spike and nucleocapsid proteins of the virus and were able to neutralize the virus. Furthermore, the UV-inactivated virion induced regional lymph node T-cell proliferation and significant levels of cytokine production (IL-2, IL-4, IL-5, IFN-γ and TNF-α) upon restimulation with inactivated SARS-CoV virion <italic>in vitro</italic>
. Thus, a whole killed virion could serve as a candidate antigen for a SARS vaccine to elicit both humoral and cellular immunity.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</pmc>
<pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice.</title>
<author><name sortKey="Takasuka, Naomi" sort="Takasuka, Naomi" uniqKey="Takasuka N" first="Naomi" last="Takasuka">Naomi Takasuka</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Fujii, Hideki" sort="Fujii, Hideki" uniqKey="Fujii H" first="Hideki" last="Fujii">Hideki Fujii</name>
</author>
<author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name>
</author>
<author><name sortKey="Kasai, Masataka" sort="Kasai, Masataka" uniqKey="Kasai M" first="Masataka" last="Kasai">Masataka Kasai</name>
</author>
<author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name>
</author>
<author><name sortKey="Itamura, Shigeyuki" sort="Itamura, Shigeyuki" uniqKey="Itamura S" first="Shigeyuki" last="Itamura">Shigeyuki Itamura</name>
</author>
<author><name sortKey="Ishii, Koji" sort="Ishii, Koji" uniqKey="Ishii K" first="Koji" last="Ishii">Koji Ishii</name>
</author>
<author><name sortKey="Sakaguchi, Masahiro" sort="Sakaguchi, Masahiro" uniqKey="Sakaguchi M" first="Masahiro" last="Sakaguchi">Masahiro Sakaguchi</name>
</author>
<author><name sortKey="Ohnishi, Kazuo" sort="Ohnishi, Kazuo" uniqKey="Ohnishi K" first="Kazuo" last="Ohnishi">Kazuo Ohnishi</name>
</author>
<author><name sortKey="Ohshima, Masamichi" sort="Ohshima, Masamichi" uniqKey="Ohshima M" first="Masamichi" last="Ohshima">Masamichi Ohshima</name>
</author>
<author><name sortKey="Hashimoto, Shu Ichi" sort="Hashimoto, Shu Ichi" uniqKey="Hashimoto S" first="Shu-Ichi" last="Hashimoto">Shu-Ichi Hashimoto</name>
</author>
<author><name sortKey="Odagiri, Takato" sort="Odagiri, Takato" uniqKey="Odagiri T" first="Takato" last="Odagiri">Takato Odagiri</name>
</author>
<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
</author>
<author><name sortKey="Yoshikura, Hiroshi" sort="Yoshikura, Hiroshi" uniqKey="Yoshikura H" first="Hiroshi" last="Yoshikura">Hiroshi Yoshikura</name>
</author>
<author><name sortKey="Takemori, Toshitada" sort="Takemori, Toshitada" uniqKey="Takemori T" first="Toshitada" last="Takemori">Toshitada Takemori</name>
</author>
<author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2004">2004</date>
<idno type="RBID">pubmed:15314040</idno>
<idno type="pmid">15314040</idno>
<idno type="doi">10.1093/intimm/dxh143</idno>
<idno type="wicri:Area/PubMed/Corpus">002C13</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002C13</idno>
<idno type="wicri:Area/PubMed/Curation">002C13</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002C13</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002E52</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002E52</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice.</title>
<author><name sortKey="Takasuka, Naomi" sort="Takasuka, Naomi" uniqKey="Takasuka N" first="Naomi" last="Takasuka">Naomi Takasuka</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Immunology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>Department of Immunology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Fujii, Hideki" sort="Fujii, Hideki" uniqKey="Fujii H" first="Hideki" last="Fujii">Hideki Fujii</name>
</author>
<author><name sortKey="Takahashi, Yoshimasa" sort="Takahashi, Yoshimasa" uniqKey="Takahashi Y" first="Yoshimasa" last="Takahashi">Yoshimasa Takahashi</name>
</author>
<author><name sortKey="Kasai, Masataka" sort="Kasai, Masataka" uniqKey="Kasai M" first="Masataka" last="Kasai">Masataka Kasai</name>
</author>
<author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name>
</author>
<author><name sortKey="Itamura, Shigeyuki" sort="Itamura, Shigeyuki" uniqKey="Itamura S" first="Shigeyuki" last="Itamura">Shigeyuki Itamura</name>
</author>
<author><name sortKey="Ishii, Koji" sort="Ishii, Koji" uniqKey="Ishii K" first="Koji" last="Ishii">Koji Ishii</name>
</author>
<author><name sortKey="Sakaguchi, Masahiro" sort="Sakaguchi, Masahiro" uniqKey="Sakaguchi M" first="Masahiro" last="Sakaguchi">Masahiro Sakaguchi</name>
</author>
<author><name sortKey="Ohnishi, Kazuo" sort="Ohnishi, Kazuo" uniqKey="Ohnishi K" first="Kazuo" last="Ohnishi">Kazuo Ohnishi</name>
</author>
<author><name sortKey="Ohshima, Masamichi" sort="Ohshima, Masamichi" uniqKey="Ohshima M" first="Masamichi" last="Ohshima">Masamichi Ohshima</name>
</author>
<author><name sortKey="Hashimoto, Shu Ichi" sort="Hashimoto, Shu Ichi" uniqKey="Hashimoto S" first="Shu-Ichi" last="Hashimoto">Shu-Ichi Hashimoto</name>
</author>
<author><name sortKey="Odagiri, Takato" sort="Odagiri, Takato" uniqKey="Odagiri T" first="Takato" last="Odagiri">Takato Odagiri</name>
</author>
<author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name>
</author>
<author><name sortKey="Yoshikura, Hiroshi" sort="Yoshikura, Hiroshi" uniqKey="Yoshikura H" first="Hiroshi" last="Yoshikura">Hiroshi Yoshikura</name>
</author>
<author><name sortKey="Takemori, Toshitada" sort="Takemori, Toshitada" uniqKey="Takemori T" first="Toshitada" last="Takemori">Toshitada Takemori</name>
</author>
<author><name sortKey="Tsunetsugu Yokota, Yasuko" sort="Tsunetsugu Yokota, Yasuko" uniqKey="Tsunetsugu Yokota Y" first="Yasuko" last="Tsunetsugu-Yokota">Yasuko Tsunetsugu-Yokota</name>
</author>
</analytic>
<series><title level="j">International immunology</title>
<idno type="ISSN">0953-8178</idno>
<imprint><date when="2004" type="published">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adjuvants, Immunologic (administration & dosage)</term>
<term>Alum Compounds (administration & dosage)</term>
<term>Animals</term>
<term>Antibody Formation</term>
<term>Antibody Specificity</term>
<term>Blotting, Western</term>
<term>Immunoglobulin G (blood)</term>
<term>Injections, Subcutaneous</term>
<term>Mice</term>
<term>SARS Virus (immunology)</term>
<term>Severe Acute Respiratory Syndrome (prevention & control)</term>
<term>Vaccines, Inactivated (administration & dosage)</term>
<term>Viral Proteins (immunology)</term>
<term>Viral Vaccines (administration & dosage)</term>
<term>Virion (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Adjuvants immunologiques (administration et posologie)</term>
<term>Alun (administration et posologie)</term>
<term>Animaux</term>
<term>Immunoglobuline G (sang)</term>
<term>Injections sous-cutanées</term>
<term>Production d'anticorps</term>
<term>Protéines virales (immunologie)</term>
<term>Souris</term>
<term>Spécificité des anticorps</term>
<term>Syndrome respiratoire aigu sévère ()</term>
<term>Technique de Western</term>
<term>Vaccins antiviraux (administration et posologie)</term>
<term>Vaccins inactivés (administration et posologie)</term>
<term>Virion (immunologie)</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Adjuvants, Immunologic</term>
<term>Alum Compounds</term>
<term>Vaccines, Inactivated</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Immunoglobulin G</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Adjuvants immunologiques</term>
<term>Alun</term>
<term>Vaccins antiviraux</term>
<term>Vaccins inactivés</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines virales</term>
<term>Virion</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
<term>Viral Proteins</term>
<term>Virion</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Immunoglobuline G</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Antibody Formation</term>
<term>Antibody Specificity</term>
<term>Blotting, Western</term>
<term>Injections, Subcutaneous</term>
<term>Mice</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Injections sous-cutanées</term>
<term>Production d'anticorps</term>
<term>Souris</term>
<term>Spécificité des anticorps</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Technique de Western</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The recent emergence of severe acute respiratory syndrome (SARS) was caused by a novel coronavirus, SARS-CoV. It spread rapidly to many countries and developing a SARS vaccine is now urgently required. In order to study the immunogenicity of UV-inactivated purified SARS-CoV virion as a vaccine candidate, we subcutaneously immunized mice with UV-inactivated SARS-CoV with or without an adjuvant. We chose aluminum hydroxide gel (alum) as an adjuvant, because of its long safety history for human use. We observed that the UV-inactivated SARS-CoV virion elicited a high level of humoral immunity, resulting in the generation of long-term antibody secreting and memory B cells. With the addition of alum to the vaccine formula, serum IgG production was augmented and reached a level similar to that found in hyper-immunized mice, though it was still insufficient to elicit serum IgA antibodies. Notably, the SARS-CoV virion itself was able to induce long-term antibody production even without an adjuvant. Anti-SARS-CoV antibodies elicited in mice recognized both the spike and nucleocapsid proteins of the virus and were able to neutralize the virus. Furthermore, the UV-inactivated virion induced regional lymph node T-cell proliferation and significant levels of cytokine production (IL-2, IL-4, IL-5, IFN-gamma and TNF-alpha) upon restimulation with inactivated SARS-CoV virion in vitro. Thus, a whole killed virion could serve as a candidate antigen for a SARS vaccine to elicit both humoral and cellular immunity.</div>
</front>
</TEI>
</pubmed>
</double>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A09 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000A09 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Ncbi |étape= Merge |type= RBID |clé= PMC:7108621 |texte= A subcutaneously injected UV-inactivated SARS coronavirus vaccine elicits systemic humoral immunity in mice }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:15314040" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |