SrasV1, Main, Exploration, bibRecord, 006717

Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis.

Identifieur interne : 006717 ( Main/Exploration ); précédent : 006716; suivant : 006718

Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis.

Auteurs : Rong Zeng [République populaire de Chine] ; Hong-Qiang Ruan ; Xiao-Sheng Jiang ; Hu Zhou ; Lv Shi ; Lei Zhang ; Quan-Hu Sheng ; Qiang Tu ; Qi-Chang Xia ; Jia-Rui Wu

Source :

Journal of proteome research [ 1535-3893 ]

RBID : pubmed:15253436

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Mass Spectrometry, Molecular Sequence Data, Nucleocapsid (metabolism), Peptides (metabolism), Phosphorylation, SARS Virus (metabolism), Viral Envelope Proteins (metabolism), Viral Matrix Proteins (metabolism).
MESH :
- chemical , metabolism : Peptides, Viral Envelope Proteins, Viral Matrix Proteins.
- metabolism : Nucleocapsid, SARS Virus.
- Amino Acid Sequence, Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Mass Spectrometry, Molecular Sequence Data, Phosphorylation.

Abstract

The proteomes of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and its infected Vero E6 cells were detected in the present study. The cytosol and nucleus fractions of virus-infected cells as well as the crude virions were analyzed either by one-dimensional electrophoresis followed by ESI-MS/MS identification or by shotgun strategy with two-dimensional liquid chromatography-ESI-MS/MS. For the first time, all of the four predicted structural proteins of SARS-CoV were identified, including S (Spike), M (Membrane), N (Nucleocapsid), and E (Envolope) proteins. In addition, a novel phosphorylated site of M protein was observed. The combination of these gel-base and non-gel methods provides fast and complimentary approaches to SARS-CoV proteome and can be widely used in the analysis of other viruses.

DOI: 10.1021/pr034111j
PubMed: 15253436

Affiliations:

République populaire de Chine

Le document en format XML

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<author><name sortKey="Sheng, Quan Hu" sort="Sheng, Quan Hu" uniqKey="Sheng Q" first="Quan-Hu" last="Sheng">Quan-Hu Sheng</name>
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<author><name sortKey="Tu, Qiang" sort="Tu, Qiang" uniqKey="Tu Q" first="Qiang" last="Tu">Qiang Tu</name>
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<term>Chromatography, Liquid</term>
<term>Electrophoresis, Polyacrylamide Gel</term>
<term>Mass Spectrometry</term>
<term>Molecular Sequence Data</term>
<term>Nucleocapsid (metabolism)</term>
<term>Peptides (metabolism)</term>
<term>Phosphorylation</term>
<term>SARS Virus (metabolism)</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Viral Matrix Proteins (metabolism)</term>
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<term>Peptides (métabolisme)</term>
<term>Phosphorylation</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Protéines de la matrice virale (métabolisme)</term>
<term>Spectrométrie de masse</term>
<term>Séquence d'acides aminés</term>
<term>Virus du SRAS (métabolisme)</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
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<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
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<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid</term>
<term>SARS Virus</term>
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<term>Peptides</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
<term>Virus du SRAS</term>
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<term>Molecular Sequence Data</term>
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<front><div type="abstract" xml:lang="en">The proteomes of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and its infected Vero E6 cells were detected in the present study. The cytosol and nucleus fractions of virus-infected cells as well as the crude virions were analyzed either by one-dimensional electrophoresis followed by ESI-MS/MS identification or by shotgun strategy with two-dimensional liquid chromatography-ESI-MS/MS. For the first time, all of the four predicted structural proteins of SARS-CoV were identified, including S (Spike), M (Membrane), N (Nucleocapsid), and E (Envolope) proteins. In addition, a novel phosphorylated site of M protein was observed. The combination of these gel-base and non-gel methods provides fast and complimentary approaches to SARS-CoV proteome and can be widely used in the analysis of other viruses.</div>
</front>
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<tree><noCountry><name sortKey="Jiang, Xiao Sheng" sort="Jiang, Xiao Sheng" uniqKey="Jiang X" first="Xiao-Sheng" last="Jiang">Xiao-Sheng Jiang</name>
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<name sortKey="Xia, Qi Chang" sort="Xia, Qi Chang" uniqKey="Xia Q" first="Qi-Chang" last="Xia">Qi-Chang Xia</name>
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<country name="République populaire de Chine"><noRegion><name sortKey="Zeng, Rong" sort="Zeng, Rong" uniqKey="Zeng R" first="Rong" last="Zeng">Rong Zeng</name>
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Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis.

Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

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