Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis.
Identifieur interne : 000948 ( Ncbi/Checkpoint ); précédent : 000947; suivant : 000949Proteomic analysis of SARS associated coronavirus using two-dimensional liquid chromatography mass spectrometry and one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by mass spectroemtric analysis.
Auteurs : Rong Zeng [République populaire de Chine] ; Hong-Qiang Ruan ; Xiao-Sheng Jiang ; Hu Zhou ; Lv Shi ; Lei Zhang ; Quan-Hu Sheng ; Qiang Tu ; Qi-Chang Xia ; Jia-Rui WuSource :
- Journal of proteome research [ 1535-3893 ]
Descripteurs français
- KwdFr :
- Chromatographie en phase liquide, Données de séquences moléculaires, Nucléocapside (métabolisme), Peptides (métabolisme), Phosphorylation, Protéines de l'enveloppe virale (métabolisme), Protéines de la matrice virale (métabolisme), Spectrométrie de masse, Séquence d'acides aminés, Virus du SRAS (métabolisme), Électrophorèse sur gel de polyacrylamide.
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Peptides, Viral Envelope Proteins, Viral Matrix Proteins.
- metabolism : Nucleocapsid, SARS Virus.
- Amino Acid Sequence, Chromatography, Liquid, Electrophoresis, Polyacrylamide Gel, Mass Spectrometry, Molecular Sequence Data, Phosphorylation.
Abstract
The proteomes of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and its infected Vero E6 cells were detected in the present study. The cytosol and nucleus fractions of virus-infected cells as well as the crude virions were analyzed either by one-dimensional electrophoresis followed by ESI-MS/MS identification or by shotgun strategy with two-dimensional liquid chromatography-ESI-MS/MS. For the first time, all of the four predicted structural proteins of SARS-CoV were identified, including S (Spike), M (Membrane), N (Nucleocapsid), and E (Envolope) proteins. In addition, a novel phosphorylated site of M protein was observed. The combination of these gel-base and non-gel methods provides fast and complimentary approaches to SARS-CoV proteome and can be widely used in the analysis of other viruses.
DOI: 10.1021/pr034111j
PubMed: 15253436
Affiliations:
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pubmed:15253436Le document en format XML
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<term>Mass Spectrometry</term>
<term>Molecular Sequence Data</term>
<term>Nucleocapsid (metabolism)</term>
<term>Peptides (metabolism)</term>
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<term>SARS Virus</term>
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<front><div type="abstract" xml:lang="en">The proteomes of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) and its infected Vero E6 cells were detected in the present study. The cytosol and nucleus fractions of virus-infected cells as well as the crude virions were analyzed either by one-dimensional electrophoresis followed by ESI-MS/MS identification or by shotgun strategy with two-dimensional liquid chromatography-ESI-MS/MS. For the first time, all of the four predicted structural proteins of SARS-CoV were identified, including S (Spike), M (Membrane), N (Nucleocapsid), and E (Envolope) proteins. In addition, a novel phosphorylated site of M protein was observed. The combination of these gel-base and non-gel methods provides fast and complimentary approaches to SARS-CoV proteome and can be widely used in the analysis of other viruses.</div>
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<tree><noCountry><name sortKey="Jiang, Xiao Sheng" sort="Jiang, Xiao Sheng" uniqKey="Jiang X" first="Xiao-Sheng" last="Jiang">Xiao-Sheng Jiang</name>
<name sortKey="Ruan, Hong Qiang" sort="Ruan, Hong Qiang" uniqKey="Ruan H" first="Hong-Qiang" last="Ruan">Hong-Qiang Ruan</name>
<name sortKey="Sheng, Quan Hu" sort="Sheng, Quan Hu" uniqKey="Sheng Q" first="Quan-Hu" last="Sheng">Quan-Hu Sheng</name>
<name sortKey="Shi, Lv" sort="Shi, Lv" uniqKey="Shi L" first="Lv" last="Shi">Lv Shi</name>
<name sortKey="Tu, Qiang" sort="Tu, Qiang" uniqKey="Tu Q" first="Qiang" last="Tu">Qiang Tu</name>
<name sortKey="Wu, Jia Rui" sort="Wu, Jia Rui" uniqKey="Wu J" first="Jia-Rui" last="Wu">Jia-Rui Wu</name>
<name sortKey="Xia, Qi Chang" sort="Xia, Qi Chang" uniqKey="Xia Q" first="Qi-Chang" last="Xia">Qi-Chang Xia</name>
<name sortKey="Zhang, Lei" sort="Zhang, Lei" uniqKey="Zhang L" first="Lei" last="Zhang">Lei Zhang</name>
<name sortKey="Zhou, Hu" sort="Zhou, Hu" uniqKey="Zhou H" first="Hu" last="Zhou">Hu Zhou</name>
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<country name="République populaire de Chine"><noRegion><name sortKey="Zeng, Rong" sort="Zeng, Rong" uniqKey="Zeng R" first="Rong" last="Zeng">Rong Zeng</name>
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