Molecular cloning and expression of a spike protein of neurovirulent murine coronavirus JHMV variant cl-2.
Identifieur interne : 006604 ( Main/Exploration ); précédent : 006603; suivant : 006605Molecular cloning and expression of a spike protein of neurovirulent murine coronavirus JHMV variant cl-2.
Auteurs : F. Taguchi [Japon] ; T. Ikeda ; H. ShidaSource :
- The Journal of general virology [ 0022-1317 ] ; 1992.
Descripteurs français
- KwdFr :
- Animaux, Anticorps monoclonaux, Cellules géantes, Clonage moléculaire, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines (biosynthèse), Glycoprotéines (génétique), Glycoprotéines (immunologie), Glycoprotéines membranaires, Protéines de l'enveloppe virale (biosynthèse), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Souris, Séquence d'acides aminés, Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (immunologie), Virus de l'hépatite murine (pathogénicité).
- MESH :
- biosynthèse : Glycoprotéines, Protéines de l'enveloppe virale.
- génétique : Glycoprotéines, Protéines de l'enveloppe virale, Virus de l'hépatite murine.
- immunologie : Glycoprotéines, Protéines de l'enveloppe virale, Virus de l'hépatite murine.
- pathogénicité : Virus de l'hépatite murine.
- Animaux, Anticorps monoclonaux, Cellules géantes, Clonage moléculaire, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Souris, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Monoclonal, Cloning, Molecular, Giant Cells, Glycoproteins (biosynthesis), Glycoproteins (genetics), Glycoproteins (immunology), Membrane Glycoproteins, Mice, Molecular Sequence Data, Murine hepatitis virus (genetics), Murine hepatitis virus (immunology), Murine hepatitis virus (pathogenicity), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , biosynthesis : Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Glycoproteins, Viral Envelope Proteins.
- chemical : Antibodies, Monoclonal, Membrane Glycoproteins, Spike Glycoprotein, Coronavirus.
- genetics : Murine hepatitis virus.
- immunology : Murine hepatitis virus.
- pathogenicity : Murine hepatitis virus.
- Amino Acid Sequence, Animals, Cloning, Molecular, Giant Cells, Mice, Molecular Sequence Data.
Abstract
A cDNA encoding the spike (S) protein of the neurovirulent murine coronavirus JHMV variant cl-2 was isolated and sequenced. Analysis of the cDNA revealed that the S protein consists of 1376 amino acids, as does the S protein of mouse hepatitis virus 4. We inserted the cDNA into the genome of vaccinia virus to obtain a recombinant vaccinia virus (rVV). The S protein expressed in RK13 cells infected by the rVV was shown to be electrophoretically and immunologically indistinguishable from the S protein produced in DBT cells infected with cl-2 virus. RVV infection of rats and mice induced S protein-specific antibody production detectable by immunofluorescence and neutralization. Moreover, the S protein expressed by the rVV induced syncytium formation not only in mouse DBT and L cells, which are susceptible to cl-2 virus infection, but also in rabbit RK13 cells, which are not susceptible to cl-2 virus infection. This result suggests the possibility that RK13 cells have binding sites for the cl-2 virus S protein.
DOI: 10.1099/0022-1317-73-5-1065
PubMed: 1316938
Affiliations:
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Le document en format XML
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Taguchi</name><affiliation wicri:level="3"><nlm:affiliation>National Institute of Neuroscience, Tokyo, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>National Institute of Neuroscience, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ikeda, T" sort="Ikeda, T" uniqKey="Ikeda T" first="T" last="Ikeda">T. Ikeda</name></author><author><name sortKey="Shida, H" sort="Shida, H" uniqKey="Shida H" first="H" last="Shida">H. Shida</name></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Monoclonal</term><term>Cloning, Molecular</term><term>Giant Cells</term><term>Glycoproteins (biosynthesis)</term><term>Glycoproteins (genetics)</term><term>Glycoproteins (immunology)</term><term>Membrane Glycoproteins</term><term>Mice</term><term>Molecular Sequence Data</term><term>Murine hepatitis virus (genetics)</term><term>Murine hepatitis virus (immunology)</term><term>Murine hepatitis virus (pathogenicity)</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (biosynthesis)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux</term><term>Cellules géantes</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines (biosynthèse)</term><term>Glycoprotéines (génétique)</term><term>Glycoprotéines (immunologie)</term><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale (biosynthèse)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Souris</term><term>Séquence d'acides aminés</term><term>Virus de l'hépatite murine (génétique)</term><term>Virus de l'hépatite murine (immunologie)</term><term>Virus de l'hépatite murine (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Glycoproteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Membrane Glycoproteins</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Glycoprotéines</term><term>Protéines de l'enveloppe virale</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines</term><term>Protéines de l'enveloppe virale</term><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines</term><term>Protéines de l'enveloppe virale</term><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Murine hepatitis virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cloning, Molecular</term><term>Giant Cells</term><term>Mice</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux</term><term>Cellules géantes</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Souris</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A cDNA encoding the spike (S) protein of the neurovirulent murine coronavirus JHMV variant cl-2 was isolated and sequenced. Analysis of the cDNA revealed that the S protein consists of 1376 amino acids, as does the S protein of mouse hepatitis virus 4. We inserted the cDNA into the genome of vaccinia virus to obtain a recombinant vaccinia virus (rVV). The S protein expressed in RK13 cells infected by the rVV was shown to be electrophoretically and immunologically indistinguishable from the S protein produced in DBT cells infected with cl-2 virus. RVV infection of rats and mice induced S protein-specific antibody production detectable by immunofluorescence and neutralization. Moreover, the S protein expressed by the rVV induced syncytium formation not only in mouse DBT and L cells, which are susceptible to cl-2 virus infection, but also in rabbit RK13 cells, which are not susceptible to cl-2 virus infection. This result suggests the possibility that RK13 cells have binding sites for the cl-2 virus S protein.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Ikeda, T" sort="Ikeda, T" uniqKey="Ikeda T" first="T" last="Ikeda">T. Ikeda</name><name sortKey="Shida, H" sort="Shida, H" uniqKey="Shida H" first="H" last="Shida">H. Shida</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Taguchi, F" sort="Taguchi, F" uniqKey="Taguchi F" first="F" last="Taguchi">F. Taguchi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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