SrasV1, PubMed, Checkpoint, bibRecord, 003441

Molecular cloning and expression of a spike protein of neurovirulent murine coronavirus JHMV variant cl-2.

Identifieur interne : 003441 ( PubMed/Checkpoint ); précédent : 003440; suivant : 003442

Molecular cloning and expression of a spike protein of neurovirulent murine coronavirus JHMV variant cl-2.

Auteurs : F. Taguchi [Japon] ; T. Ikeda ; H. Shida

Source :

The Journal of general virology [ 0022-1317 ] ; 1992.

RBID : pubmed:1316938

Descripteurs français

KwdFr :
- Animaux, Anticorps monoclonaux, Cellules géantes, Clonage moléculaire, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines (biosynthèse), Glycoprotéines (génétique), Glycoprotéines (immunologie), Glycoprotéines membranaires, Protéines de l'enveloppe virale (biosynthèse), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Souris, Séquence d'acides aminés, Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (immunologie), Virus de l'hépatite murine (pathogénicité).
MESH :
- biosynthèse : Glycoprotéines, Protéines de l'enveloppe virale.
- génétique : Glycoprotéines, Protéines de l'enveloppe virale, Virus de l'hépatite murine.
- immunologie : Glycoprotéines, Protéines de l'enveloppe virale, Virus de l'hépatite murine.
- pathogénicité : Virus de l'hépatite murine.
- Animaux, Anticorps monoclonaux, Cellules géantes, Clonage moléculaire, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Souris, Séquence d'acides aminés.

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Monoclonal, Cloning, Molecular, Giant Cells, Glycoproteins (biosynthesis), Glycoproteins (genetics), Glycoproteins (immunology), Membrane Glycoproteins, Mice, Molecular Sequence Data, Murine hepatitis virus (genetics), Murine hepatitis virus (immunology), Murine hepatitis virus (pathogenicity), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (biosynthesis), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
MESH :
- chemical , biosynthesis : Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Glycoproteins, Viral Envelope Proteins.
- chemical : Antibodies, Monoclonal, Membrane Glycoproteins, Spike Glycoprotein, Coronavirus.
- genetics : Murine hepatitis virus.
- immunology : Murine hepatitis virus.
- pathogenicity : Murine hepatitis virus.
- Amino Acid Sequence, Animals, Cloning, Molecular, Giant Cells, Mice, Molecular Sequence Data.

Abstract

A cDNA encoding the spike (S) protein of the neurovirulent murine coronavirus JHMV variant cl-2 was isolated and sequenced. Analysis of the cDNA revealed that the S protein consists of 1376 amino acids, as does the S protein of mouse hepatitis virus 4. We inserted the cDNA into the genome of vaccinia virus to obtain a recombinant vaccinia virus (rVV). The S protein expressed in RK13 cells infected by the rVV was shown to be electrophoretically and immunologically indistinguishable from the S protein produced in DBT cells infected with cl-2 virus. RVV infection of rats and mice induced S protein-specific antibody production detectable by immunofluorescence and neutralization. Moreover, the S protein expressed by the rVV induced syncytium formation not only in mouse DBT and L cells, which are susceptible to cl-2 virus infection, but also in rabbit RK13 cells, which are not susceptible to cl-2 virus infection. This result suggests the possibility that RK13 cells have binding sites for the cl-2 virus S protein.

DOI: 10.1099/0022-1317-73-5-1065
PubMed: 1316938

Affiliations:

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pubmed:1316938

Le document en format XML

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<author><name sortKey="Taguchi, F" sort="Taguchi, F" uniqKey="Taguchi F" first="F" last="Taguchi">F. Taguchi</name>
<affiliation wicri:level="3"><nlm:affiliation>National Institute of Neuroscience, Tokyo, Japan.</nlm:affiliation>
<country xml:lang="fr">Japon</country>
<wicri:regionArea>National Institute of Neuroscience, Tokyo</wicri:regionArea>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
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<author><name sortKey="Ikeda, T" sort="Ikeda, T" uniqKey="Ikeda T" first="T" last="Ikeda">T. Ikeda</name>
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<author><name sortKey="Shida, H" sort="Shida, H" uniqKey="Shida H" first="H" last="Shida">H. Shida</name>
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<term>Giant Cells</term>
<term>Glycoproteins (biosynthesis)</term>
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<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines (biosynthèse)</term>
<term>Glycoprotéines (génétique)</term>
<term>Glycoprotéines (immunologie)</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale (biosynthèse)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
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<term>Souris</term>
<term>Séquence d'acides aminés</term>
<term>Virus de l'hépatite murine (génétique)</term>
<term>Virus de l'hépatite murine (immunologie)</term>
<term>Virus de l'hépatite murine (pathogénicité)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term>
<term>Membrane Glycoproteins</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Glycoprotéines</term>
<term>Protéines de l'enveloppe virale</term>
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<term>Protéines de l'enveloppe virale</term>
<term>Virus de l'hépatite murine</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines</term>
<term>Protéines de l'enveloppe virale</term>
<term>Virus de l'hépatite murine</term>
</keywords>
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<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus de l'hépatite murine</term>
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<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Cloning, Molecular</term>
<term>Giant Cells</term>
<term>Mice</term>
<term>Molecular Sequence Data</term>
</keywords>
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<term>Anticorps monoclonaux</term>
<term>Cellules géantes</term>
<term>Clonage moléculaire</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires</term>
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<front><div type="abstract" xml:lang="en">A cDNA encoding the spike (S) protein of the neurovirulent murine coronavirus JHMV variant cl-2 was isolated and sequenced. Analysis of the cDNA revealed that the S protein consists of 1376 amino acids, as does the S protein of mouse hepatitis virus 4. We inserted the cDNA into the genome of vaccinia virus to obtain a recombinant vaccinia virus (rVV). The S protein expressed in RK13 cells infected by the rVV was shown to be electrophoretically and immunologically indistinguishable from the S protein produced in DBT cells infected with cl-2 virus. RVV infection of rats and mice induced S protein-specific antibody production detectable by immunofluorescence and neutralization. Moreover, the S protein expressed by the rVV induced syncytium formation not only in mouse DBT and L cells, which are susceptible to cl-2 virus infection, but also in rabbit RK13 cells, which are not susceptible to cl-2 virus infection. This result suggests the possibility that RK13 cells have binding sites for the cl-2 virus S protein.</div>
</front>
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<DateRevised><Year>2020</Year>
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<Abstract><AbstractText>A cDNA encoding the spike (S) protein of the neurovirulent murine coronavirus JHMV variant cl-2 was isolated and sequenced. Analysis of the cDNA revealed that the S protein consists of 1376 amino acids, as does the S protein of mouse hepatitis virus 4. We inserted the cDNA into the genome of vaccinia virus to obtain a recombinant vaccinia virus (rVV). The S protein expressed in RK13 cells infected by the rVV was shown to be electrophoretically and immunologically indistinguishable from the S protein produced in DBT cells infected with cl-2 virus. RVV infection of rats and mice induced S protein-specific antibody production detectable by immunofluorescence and neutralization. Moreover, the S protein expressed by the rVV induced syncytium formation not only in mouse DBT and L cells, which are susceptible to cl-2 virus infection, but also in rabbit RK13 cells, which are not susceptible to cl-2 virus infection. This result suggests the possibility that RK13 cells have binding sites for the cl-2 virus S protein.</AbstractText>
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Molecular cloning and expression of a spike protein of neurovirulent murine coronavirus JHMV variant cl-2.

Molecular cloning and expression of a spike protein of neurovirulent murine coronavirus JHMV variant cl-2.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki