Complex formation between the spike protein and the membrane protein during mouse hepatitis virus assembly.
Identifieur interne : 006595 ( Main/Exploration ); précédent : 006594; suivant : 006596Complex formation between the spike protein and the membrane protein during mouse hepatitis virus assembly.
Auteurs : D J Opstelten [Pays-Bas] ; M C Horzinek ; P J RottierSource :
- Advances in experimental medicine and biology [ 0065-2598 ] ; 1993.
Descripteurs français
- KwdFr :
- Animaux, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (métabolisme), Liaison aux protéines, Lignée cellulaire, Morphogenèse, Protéines de l'enveloppe virale (métabolisme), Protéines de la matrice virale (métabolisme), Virion (métabolisme), Virus de l'hépatite murine (métabolisme), Virus de l'hépatite murine (ultrastructure).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Membrane Glycoproteins, Viral Envelope Proteins, Viral Matrix Proteins.
- metabolism : Murine hepatitis virus, Virion.
- ultrastructure : Murine hepatitis virus.
- Animals, Cell Line, Morphogenesis, Protein Binding, Spike Glycoprotein, Coronavirus.
Abstract
Using different approaches we have demonstrated the formation of a complex between the S protein and the M protein in the process of mouse hepatitis virus (MHV) assembly. Preservation of the M/S heterocomplexes was critically dependent on the solubilization conditions. Pulse-chase labeling of MHV-infected cells followed by a co-immunoprecipitation assay revealed that newly synthesized S and M engage in complex formation with different kinetics, the S protein reacting much slower. Sedimentation experiments showed the M/S heteromultimer complexes to be very large. A model for the role of the complex formation in MHV assembly is presented.
DOI: 10.1007/978-1-4615-2996-5_30
PubMed: 8209729
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 003548
- to stream PubMed, to step Curation: 003548
- to stream PubMed, to step Checkpoint: 003438
- to stream Ncbi, to step Merge: 003C20
- to stream Ncbi, to step Curation: 003C20
- to stream Ncbi, to step Checkpoint: 003C20
- to stream Main, to step Merge: 006B13
- to stream Main, to step Curation: 006595
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Complex formation between the spike protein and the membrane protein during mouse hepatitis virus assembly.</title>
<author><name sortKey="Opstelten, D J" sort="Opstelten, D J" uniqKey="Opstelten D" first="D J" last="Opstelten">D J Opstelten</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht</wicri:regionArea>
<wicri:noRegion>University of Utrecht</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Horzinek, M C" sort="Horzinek, M C" uniqKey="Horzinek M" first="M C" last="Horzinek">M C Horzinek</name>
</author>
<author><name sortKey="Rottier, P J" sort="Rottier, P J" uniqKey="Rottier P" first="P J" last="Rottier">P J Rottier</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="1993">1993</date>
<idno type="RBID">pubmed:8209729</idno>
<idno type="pmid">8209729</idno>
<idno type="doi">10.1007/978-1-4615-2996-5_30</idno>
<idno type="wicri:Area/PubMed/Corpus">003548</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003548</idno>
<idno type="wicri:Area/PubMed/Curation">003548</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003548</idno>
<idno type="wicri:Area/PubMed/Checkpoint">003438</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003438</idno>
<idno type="wicri:Area/Ncbi/Merge">003C20</idno>
<idno type="wicri:Area/Ncbi/Curation">003C20</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">003C20</idno>
<idno type="wicri:doubleKey">0065-2598:1993:Opstelten D:complex:formation:between</idno>
<idno type="wicri:Area/Main/Merge">006B13</idno>
<idno type="wicri:Area/Main/Curation">006595</idno>
<idno type="wicri:Area/Main/Exploration">006595</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Complex formation between the spike protein and the membrane protein during mouse hepatitis virus assembly.</title>
<author><name sortKey="Opstelten, D J" sort="Opstelten, D J" uniqKey="Opstelten D" first="D J" last="Opstelten">D J Opstelten</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht</wicri:regionArea>
<wicri:noRegion>University of Utrecht</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Horzinek, M C" sort="Horzinek, M C" uniqKey="Horzinek M" first="M C" last="Horzinek">M C Horzinek</name>
</author>
<author><name sortKey="Rottier, P J" sort="Rottier, P J" uniqKey="Rottier P" first="P J" last="Rottier">P J Rottier</name>
</author>
</analytic>
<series><title level="j">Advances in experimental medicine and biology</title>
<idno type="ISSN">0065-2598</idno>
<imprint><date when="1993" type="published">1993</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Morphogenesis</term>
<term>Murine hepatitis virus (metabolism)</term>
<term>Murine hepatitis virus (ultrastructure)</term>
<term>Protein Binding</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Viral Matrix Proteins (metabolism)</term>
<term>Virion (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Liaison aux protéines</term>
<term>Lignée cellulaire</term>
<term>Morphogenèse</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Protéines de la matrice virale (métabolisme)</term>
<term>Virion (métabolisme)</term>
<term>Virus de l'hépatite murine (métabolisme)</term>
<term>Virus de l'hépatite murine (ultrastructure)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Murine hepatitis virus</term>
<term>Virion</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
<term>Virion</term>
<term>Virus de l'hépatite murine</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Murine hepatitis virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Morphogenesis</term>
<term>Protein Binding</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="fr"><term>Animaux</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Liaison aux protéines</term>
<term>Lignée cellulaire</term>
<term>Morphogenèse</term>
<term>Virus de l'hépatite murine</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Using different approaches we have demonstrated the formation of a complex between the S protein and the M protein in the process of mouse hepatitis virus (MHV) assembly. Preservation of the M/S heterocomplexes was critically dependent on the solubilization conditions. Pulse-chase labeling of MHV-infected cells followed by a co-immunoprecipitation assay revealed that newly synthesized S and M engage in complex formation with different kinetics, the S protein reacting much slower. Sedimentation experiments showed the M/S heteromultimer complexes to be very large. A model for the role of the complex formation in MHV assembly is presented.</div>
</front>
</TEI>
<affiliations><list><country><li>Pays-Bas</li>
</country>
</list>
<tree><noCountry><name sortKey="Horzinek, M C" sort="Horzinek, M C" uniqKey="Horzinek M" first="M C" last="Horzinek">M C Horzinek</name>
<name sortKey="Rottier, P J" sort="Rottier, P J" uniqKey="Rottier P" first="P J" last="Rottier">P J Rottier</name>
</noCountry>
<country name="Pays-Bas"><noRegion><name sortKey="Opstelten, D J" sort="Opstelten, D J" uniqKey="Opstelten D" first="D J" last="Opstelten">D J Opstelten</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006595 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 006595 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Main |étape= Exploration |type= RBID |clé= pubmed:8209729 |texte= Complex formation between the spike protein and the membrane protein during mouse hepatitis virus assembly. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:8209729" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |