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Complex formation between the spike protein and the membrane protein during mouse hepatitis virus assembly.

Identifieur interne : 003C20 ( Ncbi/Curation ); précédent : 003C19; suivant : 003C21

Complex formation between the spike protein and the membrane protein during mouse hepatitis virus assembly.

Auteurs : D J Opstelten [Pays-Bas] ; M C Horzinek ; P J Rottier

Source :

Advances in experimental medicine and biology [ 0065-2598 ] ; 1993.

RBID : pubmed:8209729

Descripteurs français

English descriptors

KwdEn :
- Animals, Cell Line, Membrane Glycoproteins (metabolism), Morphogenesis, Murine hepatitis virus (metabolism), Murine hepatitis virus (ultrastructure), Protein Binding, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (metabolism), Viral Matrix Proteins (metabolism), Virion (metabolism).
MESH :
- chemical , metabolism : Membrane Glycoproteins, Viral Envelope Proteins, Viral Matrix Proteins.
- metabolism : Murine hepatitis virus, Virion.
- ultrastructure : Murine hepatitis virus.
- Animals, Cell Line, Morphogenesis, Protein Binding, Spike Glycoprotein, Coronavirus.

Abstract

Using different approaches we have demonstrated the formation of a complex between the S protein and the M protein in the process of mouse hepatitis virus (MHV) assembly. Preservation of the M/S heterocomplexes was critically dependent on the solubilization conditions. Pulse-chase labeling of MHV-infected cells followed by a co-immunoprecipitation assay revealed that newly synthesized S and M engage in complex formation with different kinetics, the S protein reacting much slower. Sedimentation experiments showed the M/S heteromultimer complexes to be very large. A model for the role of the complex formation in MHV assembly is presented.

DOI: 10.1007/978-1-4615-2996-5_30
PubMed: 8209729

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pubmed:8209729

Le document en format XML

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<author><name sortKey="Opstelten, D J" sort="Opstelten, D J" uniqKey="Opstelten D" first="D J" last="Opstelten">D J Opstelten</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht</wicri:regionArea>
<wicri:noRegion>University of Utrecht</wicri:noRegion>
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<author><name sortKey="Horzinek, M C" sort="Horzinek, M C" uniqKey="Horzinek M" first="M C" last="Horzinek">M C Horzinek</name>
</author>
<author><name sortKey="Rottier, P J" sort="Rottier, P J" uniqKey="Rottier P" first="P J" last="Rottier">P J Rottier</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Complex formation between the spike protein and the membrane protein during mouse hepatitis virus assembly.</title>
<author><name sortKey="Opstelten, D J" sort="Opstelten, D J" uniqKey="Opstelten D" first="D J" last="Opstelten">D J Opstelten</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, University of Utrecht, The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
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<author><name sortKey="Horzinek, M C" sort="Horzinek, M C" uniqKey="Horzinek M" first="M C" last="Horzinek">M C Horzinek</name>
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<author><name sortKey="Rottier, P J" sort="Rottier, P J" uniqKey="Rottier P" first="P J" last="Rottier">P J Rottier</name>
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<series><title level="j">Advances in experimental medicine and biology</title>
<idno type="ISSN">0065-2598</idno>
<imprint><date when="1993" type="published">1993</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Morphogenesis</term>
<term>Murine hepatitis virus (metabolism)</term>
<term>Murine hepatitis virus (ultrastructure)</term>
<term>Protein Binding</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (metabolism)</term>
<term>Viral Matrix Proteins (metabolism)</term>
<term>Virion (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Liaison aux protéines</term>
<term>Lignée cellulaire</term>
<term>Morphogenèse</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Protéines de la matrice virale (métabolisme)</term>
<term>Virion (métabolisme)</term>
<term>Virus de l'hépatite murine (métabolisme)</term>
<term>Virus de l'hépatite murine (ultrastructure)</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Matrix Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Murine hepatitis virus</term>
<term>Virion</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines de la matrice virale</term>
<term>Virion</term>
<term>Virus de l'hépatite murine</term>
</keywords>
<keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Murine hepatitis virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Morphogenesis</term>
<term>Protein Binding</term>
<term>Spike Glycoprotein, Coronavirus</term>
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<term>Glycoprotéine de spicule des coronavirus</term>
<term>Liaison aux protéines</term>
<term>Lignée cellulaire</term>
<term>Morphogenèse</term>
<term>Virus de l'hépatite murine</term>
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<front><div type="abstract" xml:lang="en">Using different approaches we have demonstrated the formation of a complex between the S protein and the M protein in the process of mouse hepatitis virus (MHV) assembly. Preservation of the M/S heterocomplexes was critically dependent on the solubilization conditions. Pulse-chase labeling of MHV-infected cells followed by a co-immunoprecipitation assay revealed that newly synthesized S and M engage in complex formation with different kinetics, the S protein reacting much slower. Sedimentation experiments showed the M/S heteromultimer complexes to be very large. A model for the role of the complex formation in MHV assembly is presented.</div>
</front>
</TEI>
</record>

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Complex formation between the spike protein and the membrane protein during mouse hepatitis virus assembly.

Complex formation between the spike protein and the membrane protein during mouse hepatitis virus assembly.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki